WAT1-related protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1039 |
| AccBSI | CCGCTC | 1 cut(s) | 965 |
| AccI | GTMKAC | 1 cut(s) | 738 |
| AccII | CGCG | 1 cut(s) | 872 |
| AciI | CCGC | 3 cut(s) | 105, 965, 981 |
| AclWI | GGATC | 1 cut(s) | 1081 |
| AcoI | YGGCCR | 4 cut(s) | 102, 345, 363, 603 |
| AcsI | RAATTY | 2 cut(s) | 277, 391 |
| AcuI | CTGAAG | 1 cut(s) | 133 |
| AfaI | GTAC | 5 cut(s) | 228, 284, 814, 835, 869 |
| AflIII | ACRYGT | 2 cut(s) | 830, 870 |
| AgsI | TTSAA | 6 cut(s) | 37, 194, 391, 678, 1085, 1095 |
| AjnI | CCWGG | 1 cut(s) | 798 |
| AleI | CACNNNNGTG | 1 cut(s) | 221 |
| AluBI | AGCT | 6 cut(s) | 214, 557, 609, 686, 940, 1053 |
| AluI | AGCT | 6 cut(s) | 214, 557, 609, 686, 940, 1053 |
| Alw26I | GTCTC | 1 cut(s) | 1130 |
| AlwI | GGATC | 1 cut(s) | 1081 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 747, 1076 |
| Ama87I | CYCGRG | 2 cut(s) | 219, 1101 |
| AoxI | GGCC | 7 cut(s) | 102, 206, 345, 363, 603, 860, 884 |
| ApeKI | GCWGC | 4 cut(s) | 551, 554, 683, 940 |
| ApoI | RAATTY | 2 cut(s) | 277, 391 |
| AseI | ATTAAT | 1 cut(s) | 434 |
| AspLEI | GCGC | 1 cut(s) | 683 |
| AspS9I | GGNCC | 3 cut(s) | 495, 860, 884 |
| AsuC2I | CCSGG | 1 cut(s) | 344 |
| AsuHPI | GGTGA | 2 cut(s) | 665, 1039 |
| AvaI | CYCGRG | 2 cut(s) | 219, 1101 |
| AvaII | GGWCC | 1 cut(s) | 495 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 804, 837 |
| BalI | TGGCCA | 2 cut(s) | 365, 605 |
| BanI | GGYRCC | 1 cut(s) | 1039 |
| BbsI | GAAGAC | 1 cut(s) | 584 |
| BbvI | GCAGC | 4 cut(s) | 563, 566, 695, 927 |
| BccI | CCATC | 3 cut(s) | 107, 374, 643 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 540, 574 |
| BciT130I | CCWGG | 1 cut(s) | 800 |
| BcnI | CCSGG | 1 cut(s) | 344 |
| BcoDI | GTCTC | 1 cut(s) | 1130 |
| BfaI | CTAG | 3 cut(s) | 666, 959, 1174 |
| BfmI | CTRYAG | 2 cut(s) | 1068, 1077 |
| BisI | GCNGC | 6 cut(s) | 105, 552, 555, 684, 941, 982 |
| BlsI | GCNGC | 6 cut(s) | 106, 553, 556, 685, 942, 983 |
| Bme1390I | CCNGG | 2 cut(s) | 344, 800 |
| Bme18I | GGWCC | 1 cut(s) | 495 |
| BmeT110I | CYCGRG | 2 cut(s) | 219, 1101 |
| BmgT120I | GGNCC | 3 cut(s) | 495, 860, 884 |
| BmiI | GGNNCC | 2 cut(s) | 752, 1041 |
| BmrFI | CCNGG | 2 cut(s) | 344, 800 |
| BpiI | GAAGAC | 1 cut(s) | 584 |
| BpmI | CTGGAG | 2 cut(s) | 99, 837 |
| BpuMI | CCSGG | 1 cut(s) | 344 |
| BsaI | GGTCTC | 1 cut(s) | 1130 |
| BsaJI | CCNNGG | 4 cut(s) | 612, 799, 946, 1100 |
| Bse1I | ACTGG | 4 cut(s) | 105, 712, 752, 820 |
| Bse3DI | GCAATG | 2 cut(s) | 285, 459 |
| BseBI | CCWGG | 1 cut(s) | 800 |
| BseDI | CCNNGG | 4 cut(s) | 612, 799, 946, 1100 |
| BseGI | GGATG | 1 cut(s) | 339 |
| BseMI | GCAATG | 2 cut(s) | 285, 459 |
| BseNI | ACTGG | 4 cut(s) | 105, 712, 752, 820 |
| BseRI | GAGGAG | 2 cut(s) | 221, 1073 |
| BseXI | GCAGC | 4 cut(s) | 563, 566, 695, 927 |
| BsgI | GTGCAG | 1 cut(s) | 926 |
| Bsh1236I | CGCG | 1 cut(s) | 872 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1162 |
| BshFI | GGCC | 7 cut(s) | 104, 208, 347, 365, 605, 862, 886 |
| BshNI | GGYRCC | 1 cut(s) | 1039 |
| BsiEI | CGRYCG | 1 cut(s) | 1162 |
| BsiHKCI | CYCGRG | 2 cut(s) | 219, 1101 |
| BsiSI | CCGG | 1 cut(s) | 344 |
| BslFI | GGGAC | 3 cut(s) | 700, 758, 817 |
| BsmAI | GTCTC | 1 cut(s) | 1130 |
| BsmFI | GGGAC | 3 cut(s) | 700, 758, 817 |
| BsnI | GGCC | 7 cut(s) | 104, 208, 347, 365, 605, 862, 886 |
| Bso31I | GGTCTC | 1 cut(s) | 1130 |
| BsoBI | CYCGRG | 2 cut(s) | 219, 1101 |
| Bsp1407I | TGTACA | 1 cut(s) | 282 |
| Bsp143I | GATC | 2 cut(s) | 1073, 1159 |
| BspACI | CCGC | 3 cut(s) | 105, 965, 981 |
| BspANI | GGCC | 7 cut(s) | 104, 208, 347, 365, 605, 862, 886 |
| BspFNI | CGCG | 1 cut(s) | 872 |
| BspLI | GGNNCC | 2 cut(s) | 752, 1041 |
| BspMAI | CTGCAG | 1 cut(s) | 1072 |
| BspPI | GGATC | 1 cut(s) | 1081 |
| BspT107I | GGYRCC | 1 cut(s) | 1039 |
| BspTNI | GGTCTC | 1 cut(s) | 1130 |
| BsrBI | CCGCTC | 1 cut(s) | 965 |
| BsrDI | GCAATG | 2 cut(s) | 285, 459 |
| BsrGI | TGTACA | 1 cut(s) | 282 |
| BsrI | ACTGG | 4 cut(s) | 105, 712, 752, 820 |
| BssECI | CCNNGG | 4 cut(s) | 612, 799, 946, 1100 |
| BssMI | GATC | 2 cut(s) | 1073, 1159 |
| BssT1I | CCWWGG | 1 cut(s) | 612 |
| Bst2UI | CCWGG | 1 cut(s) | 800 |
| Bst4CI | ACNGT | 4 cut(s) | 163, 790, 808, 1141 |
| Bst6I | CTCTTC | 1 cut(s) | 191 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 317 |
| BstAUI | TGTACA | 1 cut(s) | 282 |
| BstC8I | GCNNGC | 3 cut(s) | 206, 254, 607 |
| BstDEI | CTNAG | 1 cut(s) | 645 |
| BstF5I | GGATG | 1 cut(s) | 339 |
| BstFNI | CGCG | 1 cut(s) | 872 |
| BstHHI | GCGC | 1 cut(s) | 683 |
| BstKTI | GATC | 2 cut(s) | 1076, 1162 |
| BstMAI | GTCTC | 1 cut(s) | 1130 |
| BstMBI | GATC | 2 cut(s) | 1073, 1159 |
| BstMCI | CGRYCG | 1 cut(s) | 1162 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 179, 296, 317 |
| BstNI | CCWGG | 1 cut(s) | 800 |
| BstNSI | RCATGY | 1 cut(s) | 834 |
| BstSCI | CCNGG | 2 cut(s) | 342, 798 |
| BstSFI | CTRYAG | 2 cut(s) | 1068, 1077 |
| BstUI | CGCG | 1 cut(s) | 872 |
| BstV1I | GCAGC | 4 cut(s) | 563, 566, 695, 927 |
| BstV2I | GAAGAC | 1 cut(s) | 584 |
| BstX2I | RGATCY | 1 cut(s) | 1073 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 613 |
| BstYI | RGATCY | 1 cut(s) | 1073 |
| BsuRI | GGCC | 7 cut(s) | 104, 208, 347, 365, 605, 862, 886 |
| BtsCI | GGATG | 1 cut(s) | 339 |
| Cac8I | GCNNGC | 3 cut(s) | 206, 254, 607 |
| CaiI | CAGNNNCTG | 2 cut(s) | 747, 1076 |
| CfoI | GCGC | 1 cut(s) | 683 |
| Cfr13I | GGNCC | 3 cut(s) | 495, 860, 884 |
| CseI | GACGC | 2 cut(s) | 724, 861 |
| Csp6I | GTAC | 5 cut(s) | 227, 283, 813, 834, 868 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 487, 522 |
| CviAII | CATG | 7 cut(s) | 29, 503, 518, 563, 831, 853, 1129 |
| CviQI | GTAC | 5 cut(s) | 227, 283, 813, 834, 868 |
| DdeI | CTNAG | 1 cut(s) | 645 |
| DpnI | GATC | 2 cut(s) | 1075, 1161 |
| DpnII | GATC | 2 cut(s) | 1073, 1159 |
| EaeI | YGGCCR | 4 cut(s) | 102, 345, 363, 603 |
| Eam1104I | CTCTTC | 1 cut(s) | 191 |
| EarI | CTCTTC | 1 cut(s) | 191 |
| Eco130I | CCWWGG | 1 cut(s) | 612 |
| Eco31I | GGTCTC | 1 cut(s) | 1130 |
| Eco47I | GGWCC | 1 cut(s) | 495 |
| Eco57I | CTGAAG | 1 cut(s) | 133 |
| Eco88I | CYCGRG | 2 cut(s) | 219, 1101 |
| EcoO109I | RGGNCCY | 1 cut(s) | 860 |
| EcoRII | CCWGG | 1 cut(s) | 798 |
| EcoT14I | CCWWGG | 1 cut(s) | 612 |
| ErhI | CCWWGG | 1 cut(s) | 612 |
| FaeI | CATG | 7 cut(s) | 32, 506, 521, 566, 834, 856, 1132 |
| FaqI | GGGAC | 3 cut(s) | 700, 758, 817 |
| FatI | CATG | 7 cut(s) | 28, 502, 517, 562, 830, 852, 1128 |
| FblI | GTMKAC | 1 cut(s) | 738 |
| Fnu4HI | GCNGC | 6 cut(s) | 105, 552, 555, 684, 941, 982 |
| FokI | GGATG | 1 cut(s) | 326 |
| Fsp4HI | GCNGC | 6 cut(s) | 105, 552, 555, 684, 941, 982 |
| FspBI | CTAG | 3 cut(s) | 666, 959, 1174 |
| GlaI | GCGC | 1 cut(s) | 682 |
| GluI | GCNGC | 6 cut(s) | 105, 552, 555, 684, 941, 982 |
| GsuI | CTGGAG | 2 cut(s) | 99, 837 |
| HaeIII | GGCC | 7 cut(s) | 104, 208, 347, 365, 605, 862, 886 |
| HapII | CCGG | 1 cut(s) | 344 |
| HgaI | GACGC | 2 cut(s) | 724, 861 |
| HhaI | GCGC | 1 cut(s) | 683 |
| Hin1II | CATG | 7 cut(s) | 32, 506, 521, 566, 834, 856, 1132 |
| Hin6I | GCGC | 1 cut(s) | 681 |
| HinP1I | GCGC | 1 cut(s) | 681 |
| HincII | GTYRAC | 6 cut(s) | 85, 303, 335, 508, 739, 1154 |
| HindII | GTYRAC | 6 cut(s) | 85, 303, 335, 508, 739, 1154 |
| HinfI | GANTC | 3 cut(s) | 81, 1030, 1064 |
| HpaI | GTTAAC | 1 cut(s) | 303 |
| HpaII | CCGG | 1 cut(s) | 344 |
| HphI | GGTGA | 2 cut(s) | 665, 1039 |
| Hpy166II | GTNNAC | 7 cut(s) | 69, 85, 303, 335, 508, 739, 1154 |
| Hpy188I | TCNGA | 2 cut(s) | 828, 950 |
| Hpy188III | TCNNGA | 3 cut(s) | 127, 479, 1103 |
| Hpy8I | GTNNAC | 7 cut(s) | 69, 85, 303, 335, 508, 739, 1154 |
| Hpy99I | CGWCG | 1 cut(s) | 740 |
| HpyAV | CCTTC | 2 cut(s) | 406, 1018 |
| HpyCH4III | ACNGT | 4 cut(s) | 163, 790, 808, 1141 |
| HpyCH4IV | ACGT | 1 cut(s) | 305 |
| HpyCH4V | TGCA | 7 cut(s) | 28, 290, 311, 517, 846, 943, 1070 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 179, 296, 317 |
| HpyF3I | CTNAG | 1 cut(s) | 645 |
| HpySE526I | ACGT | 1 cut(s) | 305 |
| Hsp92II | CATG | 7 cut(s) | 32, 506, 521, 566, 834, 856, 1132 |
| HspAI | GCGC | 1 cut(s) | 681 |
| KspAI | GTTAAC | 1 cut(s) | 303 |
| Kzo9I | GATC | 2 cut(s) | 1073, 1159 |
| LmnI | GCTCC | 6 cut(s) | 178, 263, 399, 449, 614, 962 |
| Lsp1109I | GCAGC | 4 cut(s) | 563, 566, 695, 927 |
| MaeI | CTAG | 3 cut(s) | 666, 959, 1174 |
| MaeII | ACGT | 1 cut(s) | 305 |
| MaeIII | GTNAC | 3 cut(s) | 270, 617, 1141 |
| MalI | GATC | 2 cut(s) | 1075, 1161 |
| MbiI | CCGCTC | 1 cut(s) | 965 |
| MboI | GATC | 2 cut(s) | 1073, 1159 |
| MboII | GAAGA | 2 cut(s) | 178, 584 |
| MflI | RGATCY | 1 cut(s) | 1073 |
| MlsI | TGGCCA | 2 cut(s) | 365, 605 |
| MluCI | AATT | 5 cut(s) | 277, 391, 633, 690, 1086 |
| MluI | ACGCGT | 1 cut(s) | 870 |
| MluNI | TGGCCA | 2 cut(s) | 365, 605 |
| MlyI | GAGTC | 1 cut(s) | 90 |
| MmeI | TCCRAC | 3 cut(s) | 360, 749, 909 |
| MnlI | CCTC | 9 cut(s) | 242, 541, 740, 851, 920, 956, 1051, 1054, 1110 |
| Mox20I | TGGCCA | 2 cut(s) | 365, 605 |
| MscI | TGGCCA | 2 cut(s) | 365, 605 |
| MseI | TTAA | 5 cut(s) | 18, 50, 302, 395, 434 |
| MslI | CAYNNNNRTG | 3 cut(s) | 221, 567, 851 |
| Msp20I | TGGCCA | 2 cut(s) | 365, 605 |
| MspI | CCGG | 1 cut(s) | 344 |
| MspR9I | CCNGG | 2 cut(s) | 344, 800 |
| MvaI | CCWGG | 1 cut(s) | 800 |
| MvnI | CGCG | 1 cut(s) | 872 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 179, 296, 317 |
| NciI | CCSGG | 1 cut(s) | 344 |
| NdeII | GATC | 2 cut(s) | 1073, 1159 |
| NlaIII | CATG | 7 cut(s) | 32, 506, 521, 566, 834, 856, 1132 |
| NlaIV | GGNNCC | 2 cut(s) | 752, 1041 |
| NmuCI | GTSAC | 2 cut(s) | 270, 1141 |
| NspI | RCATGY | 1 cut(s) | 834 |
| OliI | CACNNNNGTG | 1 cut(s) | 221 |
| PciI | ACATGT | 1 cut(s) | 830 |
| PfeI | GAWTC | 2 cut(s) | 1030, 1064 |
| PkrI | GCNGC | 6 cut(s) | 106, 553, 556, 685, 942, 983 |
| Ple19I | CGATCG | 1 cut(s) | 1162 |
| PleI | GAGTC | 1 cut(s) | 89 |
| PpsI | GAGTC | 1 cut(s) | 89 |
| PscI | ACATGT | 1 cut(s) | 830 |
| PshBI | ATTAAT | 1 cut(s) | 434 |
| Psp6I | CCWGG | 1 cut(s) | 798 |
| PspGI | CCWGG | 1 cut(s) | 798 |
| PspN4I | GGNNCC | 2 cut(s) | 752, 1041 |
| PspPI | GGNCC | 3 cut(s) | 495, 860, 884 |
| PstI | CTGCAG | 1 cut(s) | 1072 |
| PstNI | CAGNNNCTG | 2 cut(s) | 747, 1076 |
| PsuI | RGATCY | 1 cut(s) | 1073 |
| PvuI | CGATCG | 1 cut(s) | 1162 |
| RsaI | GTAC | 5 cut(s) | 228, 284, 814, 835, 869 |
| RsaNI | GTAC | 5 cut(s) | 227, 283, 813, 834, 868 |
| RseI | CAYNNNNRTG | 3 cut(s) | 221, 567, 851 |
| SalI | GTCGAC | 1 cut(s) | 737 |
| SaqAI | TTAA | 5 cut(s) | 18, 50, 302, 395, 434 |
| SatI | GCNGC | 6 cut(s) | 105, 552, 555, 684, 941, 982 |
| Sau3AI | GATC | 2 cut(s) | 1073, 1159 |
| Sau96I | GGNCC | 3 cut(s) | 495, 860, 884 |
| SchI | GAGTC | 1 cut(s) | 90 |
| ScrFI | CCNGG | 2 cut(s) | 344, 800 |
| SfcI | CTRYAG | 2 cut(s) | 1068, 1077 |
| SinI | GGWCC | 1 cut(s) | 495 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 221, 567, 851 |
| Sse9I | AATT | 5 cut(s) | 277, 391, 633, 690, 1086 |
| SsiI | CCGC | 3 cut(s) | 105, 965, 981 |
| SspI | AATATT | 1 cut(s) | 775 |
| SspMI | CTAG | 3 cut(s) | 666, 959, 1174 |
| StyD4I | CCNGG | 2 cut(s) | 342, 798 |
| StyI | CCWWGG | 1 cut(s) | 612 |
| TaaI | ACNGT | 4 cut(s) | 163, 790, 808, 1141 |
| TaiI | ACGT | 1 cut(s) | 308 |
| TaqI | TCGA | 1 cut(s) | 738 |
| TasI | AATT | 5 cut(s) | 277, 391, 633, 690, 1086 |
| TatI | WGTACW | 2 cut(s) | 282, 833 |
| TauI | GCSGC | 2 cut(s) | 107, 984 |
| TfiI | GAWTC | 2 cut(s) | 1030, 1064 |
| Tru1I | TTAA | 5 cut(s) | 18, 50, 302, 395, 434 |
| Tru9I | TTAA | 5 cut(s) | 18, 50, 302, 395, 434 |
| TseFI | GTSAC | 2 cut(s) | 270, 1141 |
| TseI | GCWGC | 4 cut(s) | 551, 554, 683, 940 |
| Tsp45I | GTSAC | 2 cut(s) | 270, 1141 |
| TspDTI | ATGAA | 1 cut(s) | 1145 |
| VpaK11BI | GGWCC | 1 cut(s) | 495 |
| VspI | ATTAAT | 1 cut(s) | 434 |
| XapI | RAATTY | 2 cut(s) | 277, 391 |
| XceI | RCATGY | 1 cut(s) | 834 |
| XmiI | GTMKAC | 1 cut(s) | 738 |
| XspI | CTAG | 3 cut(s) | 666, 959, 1174 |