RLG00000001876

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000337
Physical Location & Seq
Forward (+)
22346009 .. 22347690
1682 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001876

Sequence Viewer

Length: 984 bp
ATGGGGCAGCAGGGTGGTATTGAGATTTCTAGCAGTCCTAGTAGTGTAGTGGATGGATTAATGAAGCCAGTGATAACGATGGTGGCAGCTCAGATTGCATATGCAGCAACGGGTATTTTCTACAAACTTGCAGCAGACTCTGGAATGAACCTCAATATTCTCGTTGCCTACCGTCTCATGTTTTCCTCTGCCATTATCGTTCCTCTTGCTCTTATACTCGAAAGGAACAGCAGGCCCAAACTTACTCTAGTCGTACTCTTTCAAGCTTTTCTCTGCGGATTATTTGGGGGATCATTGTCACAAAATTTATTCATAGAGGGACTGGCCTTAACATCACCGACATTTGTTGCAGCCACTGCAAATCTGATTCCGGCGGTTACCTTGATCATGGCCATCTGTTTTAGGTTGGAGAAGTTGACACTAGGAAGCCATGCAGGGAAGGCAAAGCTTGTGGGGACAGTGGTAGGTATTGGTGGTGCTATGATCTTTACCTTCTTCAAAGGACCAGAGCTCTCCATCTGGTCAACTCACGTTGACCTTCTTCGAGGACACCACGCGGCACCATCACCTTCATCGGTCTCCTTACACAGAAGCACCAGTAGTCACTTGCTGGGCTCCTTCTCGGCTCTCGGCAGCGCCGTTTCCTATGCAATGTGGCTCACAGTTCAGGCAAAAATGAGCAAGAGATATCCATGCCCTTACTCGAGCACAGCTCTAATGTCAGTGATGGGATCGATTCAGTCGGTAGCTTTTGCACTCTGCGTGGAGAGGGATTGGAGCCAGTGGAAATTGGGTTGGAACATCAAGCTTCTCACAGCTGCATATACGGGAATTGTGTCTTCGGGGCTGGTGGTGGTTCTTATCTCGTGGTGTATACGCAAGAGAGGTCCATTGTTCGTGTCAGCTTTCACTCCGCTTTGCCTAGTGATTGTGGCCATTGCTAGTTCTTTGTTGCTCAATGAAAAGTTATGCCTAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

328

Amino Acids

34.81

Weight (kDa)

9.65

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 23 - 163 2.2e-13 EamA-like transporter family
EamA PF00892 204 - 323 4.6e-10 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 559
AccI GTMKAC 1 cut(s) 874
AccII CGCG 1 cut(s) 557
AciI CCGC 4 cut(s) 276, 374, 557, 914
AclWI GGATC 2 cut(s) 298, 739
AcoI YGGCCR 2 cut(s) 390, 933
AcsI RAATTY 1 cut(s) 304
AfaI GTAC 1 cut(s) 255
AgsI TTSAA 2 cut(s) 263, 499
AluBI AGCT 9 cut(s) 89, 266, 448, 511, 713, 749, 808, 818, 905
AluI AGCT 9 cut(s) 89, 266, 448, 511, 713, 749, 808, 818, 905
Alw21I GWGCWC 2 cut(s) 513, 710
Alw26I GTCTC 2 cut(s) 179, 583
AlwI GGATC 2 cut(s) 298, 739
AlwNI CAGNNNCTG 2 cut(s) 140, 356
Ama87I CYCGRG 1 cut(s) 703
AoxI GGCC 4 cut(s) 233, 324, 390, 933
ApeKI GCWGC 7 cut(s) 7, 86, 104, 131, 350, 633, 818
ApoI RAATTY 1 cut(s) 304
AseI ATTAAT 1 cut(s) 59
AspA2I CCTAGG 1 cut(s) 973
AspLEI GCGC 1 cut(s) 638
AspS9I GGNCC 3 cut(s) 234, 503, 887
AsuHPI GGTGA 2 cut(s) 327, 558
AvaI CYCGRG 1 cut(s) 703
AvaII GGWCC 2 cut(s) 503, 887
AvrII CCTAGG 1 cut(s) 973
BalI TGGCCA 2 cut(s) 392, 935
BanI GGYRCC 1 cut(s) 559
BanII GRGCYC 2 cut(s) 513, 617
BauI CACGAG 1 cut(s) 865
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 2 cut(s) 513, 710
BbvI GCAGC 7 cut(s) 19, 98, 116, 143, 362, 645, 805
BccI CCATC 6 cut(s) 47, 73, 401, 524, 571, 721
BceAI ACGGC 1 cut(s) 623
BclI TGATCA 1 cut(s) 384
BcoDI GTCTC 2 cut(s) 179, 583
BfaI CTAG 7 cut(s) 30, 39, 248, 422, 923, 942, 974
BfoI RGCGCY 1 cut(s) 639
BisI GCNGC 8 cut(s) 8, 87, 105, 132, 351, 558, 634, 819
BlnI CCTAGG 1 cut(s) 973
BlsI GCNGC 8 cut(s) 9, 88, 106, 133, 352, 559, 635, 820
Bme18I GGWCC 2 cut(s) 503, 887
BmeT110I CYCGRG 1 cut(s) 703
BmgT120I GGNCC 3 cut(s) 234, 503, 887
BmiI GGNNCC 3 cut(s) 561, 616, 779
BpiI GAAGAC 1 cut(s) 831
BplI GAGNNNNNCTC 2 cut(s) 697, 729
Bsa29I ATCGAT 1 cut(s) 734
BsaI GGTCTC 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 973
Bse1I ACTGG 4 cut(s) 68, 327, 597, 781
Bse3DI GCAATG 2 cut(s) 657, 936
BseCI ATCGAT 1 cut(s) 734
BseDI CCNNGG 1 cut(s) 973
BseGI GGATG 1 cut(s) 58
BseMI GCAATG 2 cut(s) 657, 936
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 4 cut(s) 68, 327, 597, 781
BseXI GCAGC 7 cut(s) 19, 98, 116, 143, 362, 645, 805
BseYI CCCAGC 1 cut(s) 610
Bsh1236I CGCG 1 cut(s) 557
BshFI GGCC 4 cut(s) 235, 326, 392, 935
BshNI GGYRCC 1 cut(s) 559
BshVI ATCGAT 1 cut(s) 734
BsiHKAI GWGCWC 2 cut(s) 513, 710
BsiHKCI CYCGRG 1 cut(s) 703
BsiSI CCGG 1 cut(s) 371
BslFI GGGAC 2 cut(s) 333, 469
BsmAI GTCTC 2 cut(s) 179, 583
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 333, 469
BsnI GGCC 4 cut(s) 235, 326, 392, 935
Bso31I GGTCTC 1 cut(s) 583
BsoBI CYCGRG 1 cut(s) 703
Bsp1286I GDGCHC 3 cut(s) 513, 617, 710
Bsp143I GATC 4 cut(s) 290, 384, 483, 731
BspACI CCGC 4 cut(s) 276, 374, 557, 914
BspANI GGCC 4 cut(s) 235, 326, 392, 935
BspCNI CTCAG 1 cut(s) 103
BspDI ATCGAT 1 cut(s) 734
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 3 cut(s) 561, 616, 779
BspPI GGATC 2 cut(s) 298, 739
BspT107I GGYRCC 1 cut(s) 559
BspTNI GGTCTC 1 cut(s) 583
BsrDI GCAATG 2 cut(s) 657, 936
BsrI ACTGG 4 cut(s) 68, 327, 597, 781
BssECI CCNNGG 1 cut(s) 973
BssMI GATC 4 cut(s) 290, 384, 483, 731
BssNAI GTATAC 1 cut(s) 875
BssSI CACGAG 1 cut(s) 865
BssT1I CCWWGG 1 cut(s) 973
Bst1107I GTATAC 1 cut(s) 875
Bst2BI CACGAG 1 cut(s) 865
Bst4CI ACNGT 3 cut(s) 173, 460, 664
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 90
BstEII GGTNACC 1 cut(s) 376
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 639
BstHHI GCGC 1 cut(s) 638
BstKTI GATC 4 cut(s) 293, 387, 486, 734
BstMAI GTCTC 2 cut(s) 179, 583
BstMBI GATC 4 cut(s) 290, 384, 483, 731
BstMWI GCNNNNNNNGC 4 cut(s) 95, 104, 356, 440
BstPI GGTNACC 1 cut(s) 376
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 7 cut(s) 19, 98, 116, 143, 362, 645, 805
BstV2I GAAGAC 1 cut(s) 831
BstZ17I GTATAC 1 cut(s) 875
Bsu15I ATCGAT 1 cut(s) 734
BsuRI GGCC 4 cut(s) 235, 326, 392, 935
BsuTUI ATCGAT 1 cut(s) 734
BtsCI GGATG 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 5 cut(s) 75, 354, 465, 729, 788
Cac8I GCNNGC 1 cut(s) 233
CaiI CAGNNNCTG 2 cut(s) 140, 356
CfoI GCGC 1 cut(s) 638
Cfr13I GGNCC 3 cut(s) 234, 503, 887
ClaI ATCGAT 1 cut(s) 734
Csp6I GTAC 1 cut(s) 254
CspCI CAANNNNNGTGG 2 cut(s) 432, 467
CviAII CATG 4 cut(s) 178, 388, 431, 693
CviQI GTAC 1 cut(s) 254
DdeI CTNAG 1 cut(s) 90
DpnI GATC 4 cut(s) 292, 386, 485, 733
DpnII GATC 4 cut(s) 290, 384, 483, 731
EaeI YGGCCR 2 cut(s) 390, 933
Ecl136II GAGCTC 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 973
Eco24I GRGCYC 2 cut(s) 513, 617
Eco31I GGTCTC 1 cut(s) 583
Eco32I GATATC 1 cut(s) 689
Eco47I GGWCC 2 cut(s) 503, 887
Eco53kI GAGCTC 1 cut(s) 511
Eco88I CYCGRG 1 cut(s) 703
Eco91I GGTNACC 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 511
EcoO65I GGTNACC 1 cut(s) 376
EcoRV GATATC 1 cut(s) 689
EcoT14I CCWWGG 1 cut(s) 973
EcoT38I GRGCYC 2 cut(s) 513, 617
ErhI CCWWGG 1 cut(s) 973
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 4 cut(s) 181, 391, 434, 696
FaqI GGGAC 2 cut(s) 333, 469
FatI CATG 4 cut(s) 177, 387, 430, 692
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 1 cut(s) 384
FblI GTMKAC 1 cut(s) 874
Fnu4HI GCNGC 8 cut(s) 8, 87, 105, 132, 351, 558, 634, 819
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 2 cut(s) 513, 617
Fsp4HI GCNGC 8 cut(s) 8, 87, 105, 132, 351, 558, 634, 819
FspBI CTAG 7 cut(s) 30, 39, 248, 422, 923, 942, 974
GlaI GCGC 1 cut(s) 637
GluI GCNGC 8 cut(s) 8, 87, 105, 132, 351, 558, 634, 819
GsaI CCCAGC 1 cut(s) 614
HaeII RGCGCY 1 cut(s) 639
HaeIII GGCC 4 cut(s) 235, 326, 392, 935
HapII CCGG 1 cut(s) 371
HhaI GCGC 1 cut(s) 638
Hin1II CATG 4 cut(s) 181, 391, 434, 696
Hin6I GCGC 1 cut(s) 636
HinP1I GCGC 1 cut(s) 636
HincII GTYRAC 3 cut(s) 417, 525, 535
HindII GTYRAC 3 cut(s) 417, 525, 535
HindIII AAGCTT 3 cut(s) 264, 446, 806
HinfI GANTC 3 cut(s) 137, 367, 736
HpaII CCGG 1 cut(s) 371
HphI GGTGA 2 cut(s) 327, 558
Hpy166II GTNNAC 4 cut(s) 417, 525, 535, 875
Hpy188I TCNGA 2 cut(s) 93, 366
Hpy188III TCNNGA 1 cut(s) 141
Hpy8I GTNNAC 4 cut(s) 417, 525, 535, 875
HpyAV CCTTC 6 cut(s) 433, 502, 548, 579, 628, 972
HpyCH4III ACNGT 3 cut(s) 173, 460, 664
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 9 cut(s) 98, 104, 131, 350, 359, 434, 650, 755, 821
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 104, 356, 440
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 531
Hsp92II CATG 4 cut(s) 181, 391, 434, 696
HspAI GCGC 1 cut(s) 636
Ksp22I TGATCA 1 cut(s) 384
Kzo9I GATC 4 cut(s) 290, 384, 483, 731
LmnI GCTCC 2 cut(s) 620, 777
Lsp1109I GCAGC 7 cut(s) 19, 98, 116, 143, 362, 645, 805
MaeI CTAG 7 cut(s) 30, 39, 248, 422, 923, 942, 974
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 3 cut(s) 297, 376, 602
MalI GATC 4 cut(s) 292, 386, 485, 733
MboI GATC 4 cut(s) 290, 384, 483, 731
MboII GAAGA 3 cut(s) 487, 533, 831
MhlI GDGCHC 3 cut(s) 513, 617, 710
MlsI TGGCCA 2 cut(s) 392, 935
MluCI AATT 3 cut(s) 304, 788, 831
MluNI TGGCCA 2 cut(s) 392, 935
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 387, 776
MnlI CCTC 7 cut(s) 161, 196, 213, 310, 539, 762, 878
Mox20I TGGCCA 2 cut(s) 392, 935
MscI TGGCCA 2 cut(s) 392, 935
MseI TTAA 2 cut(s) 59, 329
Msp20I TGGCCA 2 cut(s) 392, 935
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 1 cut(s) 371
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 4 cut(s) 95, 104, 356, 440
NdeI CATATG 1 cut(s) 100
NdeII GATC 4 cut(s) 290, 384, 483, 731
NlaIII CATG 4 cut(s) 181, 391, 434, 696
NlaIV GGNNCC 3 cut(s) 561, 616, 779
NmeAIII GCCGAG 2 cut(s) 602, 609
NmuCI GTSAC 2 cut(s) 297, 602
PaeR7I CTCGAG 1 cut(s) 703
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PfeI GAWTC 2 cut(s) 367, 736
PkrI GCNGC 8 cut(s) 9, 88, 106, 133, 352, 559, 635, 820
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 513
PspEI GGTNACC 1 cut(s) 376
PspFI CCCAGC 1 cut(s) 610
PspN4I GGNNCC 3 cut(s) 561, 616, 779
PspPI GGNCC 3 cut(s) 234, 503, 887
PspXI VCTCGAGB 1 cut(s) 703
PstNI CAGNNNCTG 2 cut(s) 140, 356
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 1 cut(s) 255
RsaNI GTAC 1 cut(s) 254
SacI GAGCTC 1 cut(s) 513
SaqAI TTAA 2 cut(s) 59, 329
SatI GCNGC 8 cut(s) 8, 87, 105, 132, 351, 558, 634, 819
Sau3AI GATC 4 cut(s) 290, 384, 483, 731
Sau96I GGNCC 3 cut(s) 234, 503, 887
SchI GAGTC 1 cut(s) 131
SduI GDGCHC 3 cut(s) 513, 617, 710
Sfr274I CTCGAG 1 cut(s) 703
SinI GGWCC 2 cut(s) 503, 887
SlaI CTCGAG 1 cut(s) 703
SmlI CTYRAG 1 cut(s) 703
SmoI CTYRAG 1 cut(s) 703
Sse9I AATT 3 cut(s) 304, 788, 831
SsiI CCGC 4 cut(s) 276, 374, 557, 914
SspI AATATT 1 cut(s) 157
SspMI CTAG 7 cut(s) 30, 39, 248, 422, 923, 942, 974
SstI GAGCTC 1 cut(s) 513
StyI CCWWGG 1 cut(s) 973
TaaI ACNGT 3 cut(s) 173, 460, 664
TaiI ACGT 1 cut(s) 534
TaqI TCGA 4 cut(s) 219, 544, 704, 734
TaqII GACCGA 1 cut(s) 565
TasI AATT 3 cut(s) 304, 788, 831
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 2 cut(s) 367, 736
Tru1I TTAA 2 cut(s) 59, 329
Tru9I TTAA 2 cut(s) 59, 329
TscAI CASTG 5 cut(s) 75, 361, 465, 729, 788
TseFI GTSAC 2 cut(s) 297, 602
TseI GCWGC 7 cut(s) 7, 86, 104, 131, 350, 633, 818
Tsp45I GTSAC 2 cut(s) 297, 602
TspDTI ATGAA 5 cut(s) 77, 161, 301, 561, 975
TspRI CASTG 5 cut(s) 75, 361, 465, 729, 788
VpaK11BI GGWCC 2 cut(s) 503, 887
VspI ATTAAT 1 cut(s) 59
XapI RAATTY 1 cut(s) 304
XhoI CTCGAG 1 cut(s) 703
XmaJI CCTAGG 1 cut(s) 973
XmiI GTMKAC 1 cut(s) 874
XspI CTAG 7 cut(s) 30, 39, 248, 422, 923, 942, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.