Rw1G034300

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000337
Physical Location & Seq
Reverse (-)
62235074 .. 62236802
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034300.1

Sequence Viewer

Length: 1095 bp
ATGGTGCAGAATCATGAGGCTTCTTCTAATTTGAAGCCGGTGATTATGATGGTGGTTACCCAGATTTTGTATTCTGTGATGAACATAATGTACAAACTAGTTGCAGTAACAGGGATGAACTTGAATGTTTTCGTTGCCTACCGACTGATGTTTTCAGCGGCCTTTATGGTTCCACTGGCTCTGATACTTGAGAGGAAGAGAAGGCCAAAACTTACTTGGGTTGTGTTGTTTCAAGCTTTTCTCTGCGGCCTAATTGGGGCTGCCGCGACACAAAACTTATATATTGTGAGTCTAGCGCTGACGTCACCCACATTTCTTGCAGCCATCTCCAATCTCCTCCCAGCCATTACCTTCGTGGTTGCTCTATGTTTTCGCCAGGAGAAGTTGACATTGGGATATTCCGGCATAGCCAAAATTGTTGGGACCGTGGTTGGGATAGGAGGAGCAATGCTTTTCACTTTCTACCGAGGACCTGTATTTACCATTTGGTCCACTCATATTGACCTTCTACGGCAATATCACAACACTAATAGCGTCTCATCCTCAAGCCACAAGAACAATCATGCGTTGGGGTTCCTGGCAGGCTTCGGCAGCATCTTGTGCTTCGGAATATGGCTCACAGTTCAGGCGAAGATGAGCAAGAGATATCCCTGTCCTTACTCGAGCACGGCTTTGATGTGCATCATGGGATCCATCCAGTCCGTTATTTTTGCGCTTTGCATGGAGAGAGATTGGAATCAATGGAAGATGGGTTGGGATATTAGGTTGTGGACTGCTGCATACTCGGGAATCATGGTTTCTGGAGTGGCAGTGGTTCTGATGTCTTGGTGTGTACAAAAGCGAGGGGCACTTTTTGTGTCAATTTTCGCTCCACTGGTACTGTTGATGGTAGCCCTTGCTAGTTCCTTGTTGTTGGAGGAAAAATCTAGCCTCGGAAGTGTATTGGGAGGAGTACTAATTGTGTGCGGGCTATACATGGTGCTGTGGGGAAAAAGCAAAGAAGAACCAACATCACTCGTACTACCCTCATCGACGATAGCTCCCCGAAATTCCAAGTCCATAGATAGCGTTCCAGTTCCAATGAGTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

364

Amino Acids

39.81

Weight (kDa)

9.61

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 13 - 153 2.5e-11 EamA-like transporter family
EamA PF00892 190 - 328 1.1e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 305
AccII CGCG 1 cut(s) 266
AciI CCGC 4 cut(s) 158, 246, 264, 966
AclWI GGATC 2 cut(s) 684, 697
AcsI RAATTY 1 cut(s) 1048
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 5 cut(s) 92, 834, 879, 954, 1020
AfeI AGCGCT 1 cut(s) 297
AfiI CCNNNNNNNGG 2 cut(s) 256, 432
AgsI TTSAA 3 cut(s) 34, 124, 233
AhlI ACTAGT 1 cut(s) 97
AjnI CCWGG 2 cut(s) 375, 576
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 2 cut(s) 236, 1040
AluI AGCT 2 cut(s) 236, 1040
Alw21I GWGCWC 1 cut(s) 668
Alw26I GTCTC 1 cut(s) 541
AlwI GGATC 2 cut(s) 684, 697
Ama87I CYCGRG 2 cut(s) 661, 784
Aor51HI AGCGCT 1 cut(s) 297
AoxI GGCC 3 cut(s) 159, 203, 247
ApeKI GCWGC 4 cut(s) 260, 320, 591, 776
ApoI RAATTY 1 cut(s) 1048
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 2 cut(s) 298, 715
AspS9I GGNCC 3 cut(s) 423, 470, 489
AsuHPI GGTGA 2 cut(s) 52, 297
AvaI CYCGRG 2 cut(s) 661, 784
AvaII GGWCC 3 cut(s) 423, 470, 489
BaeGI GKGCMC 1 cut(s) 850
BamHI GGATCC 1 cut(s) 689
Bbv12I GWGCWC 1 cut(s) 668
BbvI GCAGC 4 cut(s) 247, 332, 603, 763
BccI CCATC 5 cut(s) 43, 332, 701, 742, 880
BceAI ACGGC 2 cut(s) 527, 684
BciT130I CCWGG 2 cut(s) 377, 578
BcoDI GTCTC 1 cut(s) 541
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 4 cut(s) 98, 293, 900, 927
BfoI RGCGCY 1 cut(s) 299
BisI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
BlsI GCNGC 7 cut(s) 160, 248, 262, 265, 322, 593, 778
BmcAI AGTACT 1 cut(s) 954
Bme1390I CCNGG 2 cut(s) 377, 578
Bme18I GGWCC 3 cut(s) 423, 470, 489
BmeT110I CYCGRG 2 cut(s) 661, 784
BmgT120I GGNCC 3 cut(s) 423, 470, 489
BmiI GGNNCC 4 cut(s) 171, 424, 575, 691
BmrFI CCNGG 2 cut(s) 377, 578
BmsI GCATC 2 cut(s) 603, 690
BpmI CTGGAG 1 cut(s) 822
BpuEI CTTGAG 2 cut(s) 209, 529
BsaBI GATNNNNATC 2 cut(s) 680, 735
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 3 cut(s) 426, 466, 931
BsaXI ACNNNNNCTCC 4 cut(s) 795, 825, 1024, 1054
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 432
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 4 cut(s) 180, 697, 879, 1073
Bse3DI GCAATG 1 cut(s) 453
Bse8I GATNNNNATC 2 cut(s) 680, 735
BseBI CCWGG 2 cut(s) 377, 578
BseDI CCNNGG 3 cut(s) 426, 466, 931
BseGI GGATG 3 cut(s) 120, 539, 693
BseJI GATNNNNATC 2 cut(s) 680, 735
BseLI CCNNNNNNNGG 2 cut(s) 256, 432
BseMI GCAATG 1 cut(s) 453
BseNI ACTGG 4 cut(s) 180, 697, 879, 1073
BseRI GAGGAG 3 cut(s) 326, 456, 963
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 4 cut(s) 247, 332, 603, 763
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 3 cut(s) 161, 205, 249
BsiHKAI GWGCWC 1 cut(s) 668
BsiHKCI CYCGRG 2 cut(s) 661, 784
BsiSI CCGG 2 cut(s) 38, 402
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 2 cut(s) 256, 432
BsmAI GTCTC 1 cut(s) 541
BsmBI CGTCTC 1 cut(s) 541
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 3 cut(s) 161, 205, 249
BsoBI CYCGRG 2 cut(s) 661, 784
Bsp1286I GDGCHC 2 cut(s) 668, 850
Bsp1407I TGTACA 2 cut(s) 90, 832
Bsp143I GATC 1 cut(s) 689
BspACI CCGC 4 cut(s) 158, 246, 264, 966
BspANI GGCC 3 cut(s) 161, 205, 249
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 4 cut(s) 171, 424, 575, 691
BspPI GGATC 2 cut(s) 684, 697
BsrDI GCAATG 1 cut(s) 453
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 2 cut(s) 90, 832
BsrI ACTGG 4 cut(s) 180, 697, 879, 1073
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 426, 466, 931
BssMI GATC 1 cut(s) 689
BssNI GRCGYC 1 cut(s) 302
Bst2UI CCWGG 2 cut(s) 377, 578
Bst4CI ACNGT 3 cut(s) 427, 622, 882
Bst6I CTCTTC 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 600
BstAUI TGTACA 2 cut(s) 90, 832
BstC8I GCNNGC 2 cut(s) 583, 968
BstDSI CCRYGG 1 cut(s) 426
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 3 cut(s) 120, 539, 693
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 2 cut(s) 298, 715
BstKTI GATC 1 cut(s) 692
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 1 cut(s) 689
BstMWI GCNNNNNNNGC 2 cut(s) 591, 600
BstNI CCWGG 2 cut(s) 377, 578
BstPI GGTNACC 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 375, 576
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 4 cut(s) 247, 332, 603, 763
BstX2I RGATCY 1 cut(s) 689
BstYI RGATCY 1 cut(s) 689
BsuRI GGCC 3 cut(s) 161, 205, 249
BtgI CCRYGG 1 cut(s) 426
BtsCI GGATG 3 cut(s) 120, 539, 693
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 3 cut(s) 173, 816, 872
Cac8I GCNNGC 2 cut(s) 583, 968
CciI TCATGA 1 cut(s) 13
CfoI GCGC 2 cut(s) 298, 715
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 3 cut(s) 423, 470, 489
CseI GACGC 1 cut(s) 523
Csp6I GTAC 5 cut(s) 91, 833, 878, 953, 1019
CspCI CAANNNNNGTGG 4 cut(s) 298, 333, 481, 516
CviAII CATG 6 cut(s) 14, 563, 685, 721, 793, 976
CviQI GTAC 5 cut(s) 91, 833, 878, 953, 1019
DpnI GATC 1 cut(s) 691
DpnII GATC 1 cut(s) 689
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 647
Eco47I GGWCC 3 cut(s) 423, 470, 489
Eco47III AGCGCT 1 cut(s) 297
Eco88I CYCGRG 2 cut(s) 661, 784
Eco91I GGTNACC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 470
EcoO65I GGTNACC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 375, 576
EcoRV GATATC 1 cut(s) 647
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 6 cut(s) 17, 566, 688, 724, 796, 979
FaqI GGGAC 1 cut(s) 436
FatI CATG 6 cut(s) 13, 562, 684, 720, 792, 975
FauI CCCGC 1 cut(s) 959
Fnu4HI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
FokI GGATG 3 cut(s) 127, 526, 680
Fsp4HI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
FspBI CTAG 4 cut(s) 98, 293, 900, 927
GlaI GCGC 2 cut(s) 297, 714
GluI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
GsaI CCCAGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 3 cut(s) 161, 205, 249
HapII CCGG 2 cut(s) 38, 402
HgaI GACGC 1 cut(s) 523
HhaI GCGC 2 cut(s) 298, 715
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 6 cut(s) 17, 566, 688, 724, 796, 979
Hin6I GCGC 2 cut(s) 296, 713
HinP1I GCGC 2 cut(s) 296, 713
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HindIII AAGCTT 1 cut(s) 234
HinfI GANTC 4 cut(s) 10, 289, 736, 789
HpaII CCGG 2 cut(s) 38, 402
HphI GGTGA 2 cut(s) 52, 297
Hpy166II GTNNAC 4 cut(s) 387, 492, 771, 833
Hpy188I TCNGA 4 cut(s) 183, 608, 819, 935
Hpy188III TCNNGA 3 cut(s) 14, 786, 801
Hpy8I GTNNAC 4 cut(s) 387, 492, 771, 833
Hpy99I CGWCG 1 cut(s) 1036
HpyAV CCTTC 3 cut(s) 195, 361, 515
HpyCH4III ACNGT 3 cut(s) 427, 622, 882
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 6 cut(s) 7, 104, 320, 681, 720, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 591, 600
HpySE526I ACGT 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 6 cut(s) 17, 566, 688, 724, 796, 979
HspAI GCGC 2 cut(s) 296, 713
Kzo9I GATC 1 cut(s) 689
LmnI GCTCC 3 cut(s) 443, 874, 1045
Lsp1109I GCAGC 4 cut(s) 247, 332, 603, 763
LweI GCATC 2 cut(s) 603, 690
MaeI CTAG 4 cut(s) 98, 293, 900, 927
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 3 cut(s) 55, 106, 303
MalI GATC 1 cut(s) 691
MboI GATC 1 cut(s) 689
MboII GAAGA 5 cut(s) 15, 208, 643, 757, 1013
MflI RGATCY 1 cut(s) 689
MhlI GDGCHC 2 cut(s) 668, 850
MluCI AATT 6 cut(s) 28, 252, 414, 861, 957, 1048
MlyI GAGTC 1 cut(s) 298
MmeI TCCRAC 2 cut(s) 894, 1069
MroXI GAANNNNTTC 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 2 cut(s) 38, 402
MspR9I CCNGG 2 cut(s) 377, 578
MvaI CCWGG 2 cut(s) 377, 578
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 591, 600
NdeII GATC 1 cut(s) 689
NlaIII CATG 6 cut(s) 17, 566, 688, 724, 796, 979
NlaIV GGNNCC 4 cut(s) 171, 424, 575, 691
NmuCI GTSAC 1 cut(s) 303
PaeR7I CTCGAG 1 cut(s) 661
PagI TCATGA 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 3 cut(s) 10, 736, 789
PkrI GCNGC 7 cut(s) 160, 248, 262, 265, 322, 593, 778
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
PpuMI RGGWCCY 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 2 cut(s) 375, 576
PspEI GGTNACC 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 2 cut(s) 375, 576
PspN4I GGNNCC 4 cut(s) 171, 424, 575, 691
PspPI GGNCC 3 cut(s) 423, 470, 489
PspPPI RGGWCCY 1 cut(s) 470
PspXI VCTCGAGB 1 cut(s) 661
PsrI GAACNNNNNNTAC 2 cut(s) 74, 106
PsuI RGATCY 1 cut(s) 689
RsaI GTAC 5 cut(s) 92, 834, 879, 954, 1020
RsaNI GTAC 5 cut(s) 91, 833, 878, 953, 1019
SatI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
Sau3AI GATC 1 cut(s) 689
Sau96I GGNCC 3 cut(s) 423, 470, 489
ScaI AGTACT 1 cut(s) 954
SchI GAGTC 1 cut(s) 298
ScrFI CCNGG 2 cut(s) 377, 578
SduI GDGCHC 2 cut(s) 668, 850
SetI ASST 7 cut(s) 238, 305, 353, 475, 507, 767, 1042
SfaNI GCATC 2 cut(s) 603, 690
Sfr274I CTCGAG 1 cut(s) 661
SinI GGWCC 3 cut(s) 423, 470, 489
SlaI CTCGAG 1 cut(s) 661
SmlI CTYRAG 3 cut(s) 188, 544, 661
SmoI CTYRAG 3 cut(s) 188, 544, 661
SpeI ACTAGT 1 cut(s) 97
Sse9I AATT 6 cut(s) 28, 252, 414, 861, 957, 1048
SsiI CCGC 4 cut(s) 158, 246, 264, 966
SspMI CTAG 4 cut(s) 98, 293, 900, 927
StyD4I CCNGG 2 cut(s) 375, 576
TaaI ACNGT 3 cut(s) 427, 622, 882
TaiI ACGT 1 cut(s) 305
TaqI TCGA 2 cut(s) 662, 1031
TasI AATT 6 cut(s) 28, 252, 414, 861, 957, 1048
TatI WGTACW 3 cut(s) 90, 832, 952
TauI GCSGC 3 cut(s) 161, 249, 266
TfiI GAWTC 3 cut(s) 10, 736, 789
TscAI CASTG 3 cut(s) 180, 816, 879
TseFI GTSAC 1 cut(s) 303
TseI GCWGC 4 cut(s) 260, 320, 591, 776
Tsp45I GTSAC 1 cut(s) 303
TspDTI ATGAA 2 cut(s) 95, 131
TspGWI ACGGA 1 cut(s) 691
TspRI CASTG 3 cut(s) 180, 816, 879
VpaK11BI GGWCC 3 cut(s) 423, 470, 489
XapI RAATTY 1 cut(s) 1048
XcmI CCANNNNNNNNNTGG 2 cut(s) 213, 352
XhoI CTCGAG 1 cut(s) 661
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 4 cut(s) 98, 293, 900, 927
ZraI GACGTC 1 cut(s) 303
ZrmI AGTACT 1 cut(s) 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.