MD15G1417500.v1.1

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000337
Physical Location & Seq
Forward (+)
51927189 .. 51931133
3945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1417500.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGTTACATGGGTTGAAACCAATGTTGGTAATGGTGTTGGTTCAAATTTTAAGTGCAGCACTGAATATAATTTTCAAACTGGTAGCAAACGATGGGATGAGTTTAAAGGTCTTCACTGCCTATCGTGTCCTTTTTGCCTCTGTCTTTATGATACCTCTTGCTCTTGTACTTGAAAGCCTATCACTAGCTCAAAATTTGTTCATGGAGAGCATTGCGTTTGCGTCAGCAACATATTGTGTGGCGATAACTAATCTGATTCCGGCAATAACGTTCCTACTGGCGGTATGCTTTCGGCAGGAGAAGCTTTCGTTAACAAGTATTCCAGGAATGGCAAAACTTGCCGGAACAGCGGTTGGGTTGGGAGGGGCAATGGTCTTCGTATTCTACAAAGGCATCACTATAAAAATTTGGTCAGTTCATGGTAACCTCATAGACACGAAGAACAAAACCACTTCATCGTCGTCGCACCACCCCACCAATTATGCCTTGGGTGCAGTGTTAGCTTTCCTGGGCTGTACGTGCAGTTCACTATGGCTCATAATTCAGGGAAAAATGACTAGAACATTCCCGTGCCCCATGTCGGCCACAGCTTTGACATCGCTAATGGCAACAATTCAATCTGTGATTTATGCCCTTTGCGCGGAGAGGGATTGGAAGCAATGGCATTTGGGCTGGAATATTAGACTACTTGGAGTTGTTTATTCGGGAATAGTGATTTCAGGAATTGCGGTGACCTTGATGGCATGGTGTATAAACATGCGAGGGCCATTGTATGCAGCGAGTTTCTTTCCTGTCCTACTTGTACTGGTTGCCATACTTGCCTCATTGTTGCTGGAAGAGAACTTGTACCTGGGAAGCTTACTAGGAGCAGTGTTCATCATAGGTGGGTTATACCTATATTTGTGGGGTAAGAGCATCGAAATAAAGAGGATGAATCGGGTAGAACCACTCGAAACCCCCCGACCTTCAGAATTTCAGTCTATTGAAATCGTTACAACATCAACATCTGCTCCGGCCATTACCACCGGGAGTAATGACAACAACATCACAAGTAATAGCAGCAGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

357

Amino Acids

38.58

Weight (kDa)

9.16

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 163 - 301 1.6e-11 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 641
AciI CCGC 4 cut(s) 281, 350, 641, 728
AclI AACGTT 1 cut(s) 269
AcoI YGGCCR 2 cut(s) 582, 1014
AcsI RAATTY 4 cut(s) 45, 193, 405, 971
AcuI CTGAAG 1 cut(s) 951
AfaI GTAC 4 cut(s) 168, 517, 804, 848
AfiI CCNNNNNNNGG 3 cut(s) 280, 580, 640
AgsI TTSAA 6 cut(s) 16, 44, 76, 173, 617, 986
AjnI CCWGG 3 cut(s) 322, 507, 849
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AluBI AGCT 5 cut(s) 188, 304, 503, 590, 858
AluI AGCT 5 cut(s) 188, 304, 503, 590, 858
AoxI GGCC 3 cut(s) 582, 764, 1014
ApeKI GCWGC 3 cut(s) 56, 776, 1059
ApoI RAATTY 4 cut(s) 45, 193, 405, 971
AspLEI GCGC 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 764
AsuC2I CCSGG 1 cut(s) 1027
AsuHPI GGTGA 1 cut(s) 742
BaeGI GKGCMC 1 cut(s) 575
BbsI GAAGAC 2 cut(s) 103, 367
BbvI GCAGC 2 cut(s) 68, 788
BccI CCATC 2 cut(s) 86, 733
BciT130I CCWGG 3 cut(s) 324, 509, 851
BcnI CCSGG 1 cut(s) 1027
BfaI CTAG 3 cut(s) 185, 558, 863
BisI GCNGC 3 cut(s) 57, 777, 1060
BlsI GCNGC 3 cut(s) 58, 778, 1061
Bme1390I CCNGG 4 cut(s) 324, 509, 851, 1027
BmgT120I GGNCC 1 cut(s) 764
BmrFI CCNGG 4 cut(s) 324, 509, 851, 1027
BmsI GCATC 2 cut(s) 402, 924
BpiI GAAGAC 2 cut(s) 103, 367
BpuMI CCSGG 1 cut(s) 1027
BsaAI YACGTR 1 cut(s) 519
BsaJI CCNNGG 3 cut(s) 486, 508, 850
BsaXI ACNNNNNCTCC 2 cut(s) 994, 1024
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 580, 640
Bse1I ACTGG 3 cut(s) 84, 282, 810
Bse3DI GCAATG 3 cut(s) 210, 375, 665
BseBI CCWGG 3 cut(s) 324, 509, 851
BseDI CCNNGG 3 cut(s) 486, 508, 850
BseGI GGATG 2 cut(s) 102, 936
BseLI CCNNNNNNNGG 3 cut(s) 280, 580, 640
BseMI GCAATG 3 cut(s) 210, 375, 665
BseNI ACTGG 3 cut(s) 84, 282, 810
BseSI GKGCMC 1 cut(s) 575
BseXI GCAGC 2 cut(s) 68, 788
BsgI GTGCAG 3 cut(s) 75, 513, 541
Bsh1236I CGCG 1 cut(s) 641
BshFI GGCC 3 cut(s) 584, 766, 1016
BsiSI CCGG 4 cut(s) 260, 342, 1013, 1026
BslI CCNNNNNNNGG 3 cut(s) 280, 580, 640
BsnI GGCC 3 cut(s) 584, 766, 1016
Bsp1286I GDGCHC 1 cut(s) 575
BspACI CCGC 4 cut(s) 281, 350, 641, 728
BspANI GGCC 3 cut(s) 584, 766, 1016
BspFNI CGCG 1 cut(s) 641
BsrDI GCAATG 3 cut(s) 210, 375, 665
BsrI ACTGG 3 cut(s) 84, 282, 810
BssECI CCNNGG 3 cut(s) 486, 508, 850
BssT1I CCWWGG 1 cut(s) 486
Bst2UI CCWGG 3 cut(s) 324, 509, 851
Bst6I CTCTTC 1 cut(s) 831
BstAPI GCANNNNNTGC 1 cut(s) 338
BstBAI YACGTR 1 cut(s) 519
BstEII GGTNACC 2 cut(s) 422, 730
BstF5I GGATG 2 cut(s) 102, 936
BstFNI CGCG 1 cut(s) 641
BstHHI GCGC 1 cut(s) 641
BstMWI GCNNNNNNNGC 8 cut(s) 301, 338, 347, 491, 500, 519, 638, 818
BstNI CCWGG 3 cut(s) 324, 509, 851
BstNSI RCATGY 1 cut(s) 760
BstPI GGTNACC 2 cut(s) 422, 730
BstSCI CCNGG 4 cut(s) 322, 507, 849, 1025
BstSLI GKGCMC 1 cut(s) 575
BstUI CGCG 1 cut(s) 641
BstV1I GCAGC 2 cut(s) 68, 788
BstV2I GAAGAC 2 cut(s) 103, 367
BsuRI GGCC 3 cut(s) 584, 766, 1016
BtgZI GCGATG 1 cut(s) 582
BtsCI GGATG 2 cut(s) 102, 936
BtsI GCAGTG 3 cut(s) 114, 501, 876
BtsIMutI CAGTG 4 cut(s) 59, 114, 501, 876
CfoI GCGC 1 cut(s) 641
Cfr13I GGNCC 1 cut(s) 764
CseI GACGC 1 cut(s) 210
Csp6I GTAC 4 cut(s) 167, 516, 803, 847
CviAII CATG 6 cut(s) 8, 202, 419, 577, 744, 757
CviQI GTAC 4 cut(s) 167, 516, 803, 847
DraI TTTAAA 1 cut(s) 105
EaeI YGGCCR 2 cut(s) 582, 1014
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
Eco130I CCWWGG 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 951
Eco91I GGTNACC 2 cut(s) 422, 730
EcoO65I GGTNACC 2 cut(s) 422, 730
EcoRII CCWGG 3 cut(s) 322, 507, 849
EcoT14I CCWWGG 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 6 cut(s) 11, 205, 422, 580, 747, 760
FatI CATG 6 cut(s) 7, 201, 418, 576, 743, 756
Fnu4HI GCNGC 3 cut(s) 57, 777, 1060
FokI GGATG 2 cut(s) 109, 943
Fsp4HI GCNGC 3 cut(s) 57, 777, 1060
FspBI CTAG 3 cut(s) 185, 558, 863
GlaI GCGC 1 cut(s) 640
GluI GCNGC 3 cut(s) 57, 777, 1060
HaeIII GGCC 3 cut(s) 584, 766, 1016
HapII CCGG 4 cut(s) 260, 342, 1013, 1026
HgaI GACGC 1 cut(s) 210
HhaI GCGC 1 cut(s) 641
Hin1II CATG 6 cut(s) 11, 205, 422, 580, 747, 760
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 2 cut(s) 302, 856
HinfI GANTC 2 cut(s) 256, 934
HpaI GTTAAC 1 cut(s) 312
HpaII CCGG 4 cut(s) 260, 342, 1013, 1026
HphI GGTGA 1 cut(s) 742
Hpy166II GTNNAC 2 cut(s) 312, 527
Hpy188I TCNGA 2 cut(s) 255, 970
Hpy188III TCNNGA 2 cut(s) 705, 720
Hpy8I GTNNAC 2 cut(s) 312, 527
Hpy99I CGWCG 2 cut(s) 463, 466
HpyAV CCTTC 1 cut(s) 975
HpyCH4IV ACGT 2 cut(s) 269, 518
HpyCH4V TGCA 4 cut(s) 56, 494, 522, 776
HpyF10VI GCNNNNNNNGC 8 cut(s) 301, 338, 347, 491, 500, 519, 638, 818
HpySE526I ACGT 2 cut(s) 269, 518
Hsp92II CATG 6 cut(s) 11, 205, 422, 580, 747, 760
HspAI GCGC 1 cut(s) 639
KspAI GTTAAC 1 cut(s) 312
LmnI GCTCC 2 cut(s) 866, 1015
Lsp1109I GCAGC 2 cut(s) 68, 788
LweI GCATC 2 cut(s) 402, 924
MaeI CTAG 3 cut(s) 185, 558, 863
MaeII ACGT 2 cut(s) 269, 518
MaeIII GTNAC 4 cut(s) 3, 422, 730, 991
MboII GAAGA 4 cut(s) 103, 367, 451, 848
MhlI GDGCHC 1 cut(s) 575
MluCI AATT 9 cut(s) 45, 69, 193, 405, 478, 540, 612, 723, 971
MnlI CCTC 9 cut(s) 148, 165, 356, 437, 639, 755, 832, 921, 1058
MseI TTAA 4 cut(s) 50, 104, 311, 1069
MslI CAYNNNNRTG 1 cut(s) 568
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 4 cut(s) 260, 342, 1013, 1026
MspR9I CCNGG 4 cut(s) 324, 509, 851, 1027
MvaI CCWGG 3 cut(s) 324, 509, 851
MvnI CGCG 1 cut(s) 641
MwoI GCNNNNNNNGC 8 cut(s) 301, 338, 347, 491, 500, 519, 638, 818
NciI CCSGG 1 cut(s) 1027
NlaIII CATG 6 cut(s) 11, 205, 422, 580, 747, 760
NmuCI GTSAC 1 cut(s) 730
NspI RCATGY 1 cut(s) 760
PfeI GAWTC 2 cut(s) 256, 934
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 3 cut(s) 58, 778, 1061
Ppu21I YACGTR 1 cut(s) 519
Psp1406I AACGTT 1 cut(s) 269
Psp6I CCWGG 3 cut(s) 322, 507, 849
PspEI GGTNACC 2 cut(s) 422, 730
PspGI CCWGG 3 cut(s) 322, 507, 849
PspPI GGNCC 1 cut(s) 764
PsrI GAACNNNNNNTAC 2 cut(s) 508, 540
RsaI GTAC 4 cut(s) 168, 517, 804, 848
RsaNI GTAC 4 cut(s) 167, 516, 803, 847
RseI CAYNNNNRTG 1 cut(s) 568
SaqAI TTAA 4 cut(s) 50, 104, 311, 1069
SatI GCNGC 3 cut(s) 57, 777, 1060
Sau96I GGNCC 1 cut(s) 764
ScrFI CCNGG 4 cut(s) 324, 509, 851, 1027
SduI GDGCHC 1 cut(s) 575
SfaNI GCATC 2 cut(s) 402, 924
SmiMI CAYNNNNRTG 1 cut(s) 568
Sse9I AATT 9 cut(s) 45, 69, 193, 405, 478, 540, 612, 723, 971
SsiI CCGC 4 cut(s) 281, 350, 641, 728
SspI AATATT 1 cut(s) 679
SspMI CTAG 3 cut(s) 185, 558, 863
StyD4I CCNGG 4 cut(s) 322, 507, 849, 1025
StyI CCWWGG 1 cut(s) 486
TaiI ACGT 2 cut(s) 272, 521
TaqI TCGA 2 cut(s) 918, 951
TasI AATT 9 cut(s) 45, 69, 193, 405, 478, 540, 612, 723, 971
TatI WGTACW 2 cut(s) 166, 802
TfiI GAWTC 2 cut(s) 256, 934
Tru1I TTAA 4 cut(s) 50, 104, 311, 1069
Tru9I TTAA 4 cut(s) 50, 104, 311, 1069
TscAI CASTG 4 cut(s) 66, 121, 501, 876
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 3 cut(s) 56, 776, 1059
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 5 cut(s) 190, 407, 444, 865, 947
TspRI CASTG 4 cut(s) 66, 121, 501, 876
XapI RAATTY 4 cut(s) 45, 193, 405, 971
XceI RCATGY 1 cut(s) 760
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XspI CTAG 3 cut(s) 185, 558, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.