RchiOBHm_Chr7g0224611

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000337
Physical Location & Seq
Reverse (-)
46880430 .. 46882432
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20113

Sequence Viewer

Length: 1104 bp
ATGAAAAAATTTCAGGTTGAAGGGTATCTCTCTGTCTCTCTCTGTCTCTCTCTCTCTCTCTGCATTTTGAAGCCAGTAATACAAATGGGGCAGCAGGGTGGTATTGAGATTTCTAGCAGTCCTAGTAGTGTAGTGGATGGATTAATGAAGCCAGTGATAACGATGGTGGCAGCTCAGATTGCATATGCAGCAACGGGTATTTTCTACAAACTTGCAGCAGACTCTGGAATGAACCTCAATATTCTCGTTGCCTACCGTCTCATGTTTTCCTCTGCCATTATCGTTCCTCTTGCTCTTATACTCGAAAGGGGATCATTGTCACAAAATTTATTCATAGAGGGACTGGCCTTAACATCACCGACATTTGTTGCAGCCACTGCAAATCTGATTCCGGCGGTTACCTTGATCATGGCCATCTGTTTTAGGTTGGAGAAGTTGACACTAGGAAGCCATGCAGGGAAGGCAAAGCTTGTGGGGACAGTGGTAGGTATTGGTGGTGCTATGATCTTTACCTTCTTCAAAGGACCAGAGCTCTCCATCTGGTCAACTCACGTTGACCTTCTTCGAGGACACCACGCGGCACCATCACCGTCATCGGTCTCCTTACACAGAAGCACCGGTAGTCACTTGCTGGGCTCCTTCTCGGCTCTCGGCAGCGCCGTTTCCTATGCAATGTGGCTCACAGTTCAGGCAAAAATGAGCAAGAGATATCCATGCCCTTACTCGAGCACAGCTCTAATGTCAGTGATGGGATCGATTCAGTCGGTAGCTTTTGCACTCTGCTTGGAGAGGGATTGGAGCCAGTGGAAATTGGGTTGGAACATCAAGCTTCTCACAGCTGCATATACGGGAATTGTGTCTTCGGGGCTGGTGGTGGTTCTTATCTCGTGGTGTATACGCAAGAGAGGTCCATTGTTCGTGTCAGCTTTCACTCCGCTTTGCCTCGTGATTGTGGCCATTGCTAGTTCTTTGTTGATCAATGAAAAGTTAAGCCTAGGAAGCATATTAGGAGGGATTCTAATTGTATGTGGGTTATATATGGTGCTATGGGGAAAGAGCAAGGAGATGAAGTCGATTAATAATTTAGACAGTGATCAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

367

Amino Acids

39.17

Weight (kDa)

9.31

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 211 - 349 5.6e-14 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 580
AccI GTMKAC 1 cut(s) 895
AccII CGCG 1 cut(s) 578
AciI CCGC 3 cut(s) 395, 578, 935
AclWI GGATC 2 cut(s) 319, 760
AcoI YGGCCR 2 cut(s) 411, 954
AcsI RAATTY 2 cut(s) 8, 325
AgeI ACCGGT 1 cut(s) 617
AgsI TTSAA 3 cut(s) 20, 70, 520
AluBI AGCT 8 cut(s) 173, 469, 532, 734, 770, 829, 839, 926
AluI AGCT 8 cut(s) 173, 469, 532, 734, 770, 829, 839, 926
Alw21I GWGCWC 2 cut(s) 534, 731
Alw26I GTCTC 4 cut(s) 40, 50, 263, 604
AlwI GGATC 2 cut(s) 319, 760
AlwNI CAGNNNCTG 2 cut(s) 224, 377
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 3 cut(s) 345, 411, 954
ApeKI GCWGC 7 cut(s) 91, 170, 188, 215, 371, 654, 839
ApoI RAATTY 2 cut(s) 8, 325
AseI ATTAAT 2 cut(s) 143, 1077
AsiGI ACCGGT 1 cut(s) 617
AspA2I CCTAGG 1 cut(s) 994
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 2 cut(s) 524, 908
AsuHPI GGTGA 2 cut(s) 348, 579
AvaI CYCGRG 1 cut(s) 724
AvaII GGWCC 2 cut(s) 524, 908
AvrII CCTAGG 1 cut(s) 994
BalI TGGCCA 2 cut(s) 413, 956
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 2 cut(s) 534, 638
BauI CACGAG 2 cut(s) 886, 944
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 2 cut(s) 534, 731
BbvI GCAGC 7 cut(s) 103, 182, 200, 227, 383, 666, 826
BccI CCATC 6 cut(s) 131, 157, 422, 545, 592, 742
BceAI ACGGC 1 cut(s) 644
BclI TGATCA 3 cut(s) 405, 975, 1093
BcoDI GTCTC 4 cut(s) 40, 50, 263, 604
BfaI CTAG 5 cut(s) 114, 123, 443, 963, 995
BfoI RGCGCY 1 cut(s) 660
BisI GCNGC 8 cut(s) 92, 171, 189, 216, 372, 579, 655, 840
BlnI CCTAGG 1 cut(s) 994
BlsI GCNGC 8 cut(s) 93, 172, 190, 217, 373, 580, 656, 841
Bme18I GGWCC 2 cut(s) 524, 908
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 524, 908
BmiI GGNNCC 3 cut(s) 582, 637, 800
BpiI GAAGAC 1 cut(s) 852
BplI GAGNNNNNCTC 2 cut(s) 718, 750
Bsa29I ATCGAT 1 cut(s) 755
BsaI GGTCTC 1 cut(s) 604
BsaJI CCNNGG 1 cut(s) 994
BsaWI WCCGGW 1 cut(s) 617
BsaXI ACNNNNNCTCC 2 cut(s) 1055, 1085
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 4 cut(s) 74, 152, 348, 802
Bse3DI GCAATG 2 cut(s) 678, 957
BseCI ATCGAT 1 cut(s) 755
BseDI CCNNGG 1 cut(s) 994
BseGI GGATG 1 cut(s) 142
BseMI GCAATG 2 cut(s) 678, 957
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 4 cut(s) 74, 152, 348, 802
BseXI GCAGC 7 cut(s) 103, 182, 200, 227, 383, 666, 826
BseYI CCCAGC 1 cut(s) 631
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 3 cut(s) 347, 413, 956
BshNI GGYRCC 1 cut(s) 580
BshTI ACCGGT 1 cut(s) 617
BshVI ATCGAT 1 cut(s) 755
BsiHKAI GWGCWC 2 cut(s) 534, 731
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 2 cut(s) 392, 618
BslFI GGGAC 2 cut(s) 354, 490
BsmAI GTCTC 4 cut(s) 40, 50, 263, 604
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 354, 490
BsnI GGCC 3 cut(s) 347, 413, 956
Bso31I GGTCTC 1 cut(s) 604
BsoBI CYCGRG 1 cut(s) 724
Bsp1286I GDGCHC 3 cut(s) 534, 638, 731
Bsp143I GATC 6 cut(s) 311, 405, 504, 752, 975, 1093
BspACI CCGC 3 cut(s) 395, 578, 935
BspANI GGCC 3 cut(s) 347, 413, 956
BspCNI CTCAG 1 cut(s) 187
BspDI ATCGAT 1 cut(s) 755
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 3 cut(s) 582, 637, 800
BspPI GGATC 2 cut(s) 319, 760
BspT107I GGYRCC 1 cut(s) 580
BspTNI GGTCTC 1 cut(s) 604
BsrDI GCAATG 2 cut(s) 678, 957
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 4 cut(s) 74, 152, 348, 802
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 1 cut(s) 994
BssMI GATC 6 cut(s) 311, 405, 504, 752, 975, 1093
BssNAI GTATAC 1 cut(s) 896
BssSI CACGAG 2 cut(s) 886, 944
BssT1I CCWWGG 1 cut(s) 994
Bst1107I GTATAC 1 cut(s) 896
Bst2BI CACGAG 2 cut(s) 886, 944
Bst4CI ACNGT 5 cut(s) 257, 481, 591, 685, 1091
BstAPI GCANNNNNTGC 1 cut(s) 377
BstDEI CTNAG 1 cut(s) 174
BstEII GGTNACC 1 cut(s) 397
BstF5I GGATG 1 cut(s) 142
BstFNI CGCG 1 cut(s) 578
BstH2I RGCGCY 1 cut(s) 660
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 6 cut(s) 314, 408, 507, 755, 978, 1096
BstMAI GTCTC 4 cut(s) 40, 50, 263, 604
BstMBI GATC 6 cut(s) 311, 405, 504, 752, 975, 1093
BstMWI GCNNNNNNNGC 5 cut(s) 179, 188, 377, 461, 999
BstPI GGTNACC 1 cut(s) 397
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 7 cut(s) 103, 182, 200, 227, 383, 666, 826
BstV2I GAAGAC 1 cut(s) 852
BstZ17I GTATAC 1 cut(s) 896
Bsu15I ATCGAT 1 cut(s) 755
BsuRI GGCC 3 cut(s) 347, 413, 956
BsuTUI ATCGAT 1 cut(s) 755
BtsCI GGATG 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 6 cut(s) 159, 375, 486, 750, 809, 1096
CaiI CAGNNNCTG 2 cut(s) 224, 377
CfoI GCGC 1 cut(s) 659
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 2 cut(s) 524, 908
ClaI ATCGAT 1 cut(s) 755
CspAI ACCGGT 1 cut(s) 617
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 4 cut(s) 262, 409, 452, 714
DdeI CTNAG 1 cut(s) 174
DpnI GATC 6 cut(s) 313, 407, 506, 754, 977, 1095
DpnII GATC 6 cut(s) 311, 405, 504, 752, 975, 1093
EaeI YGGCCR 2 cut(s) 411, 954
Ecl136II GAGCTC 1 cut(s) 532
Eco130I CCWWGG 1 cut(s) 994
Eco24I GRGCYC 2 cut(s) 534, 638
Eco31I GGTCTC 1 cut(s) 604
Eco32I GATATC 1 cut(s) 710
Eco47I GGWCC 2 cut(s) 524, 908
Eco53kI GAGCTC 1 cut(s) 532
Eco88I CYCGRG 1 cut(s) 724
Eco91I GGTNACC 1 cut(s) 397
EcoICRI GAGCTC 1 cut(s) 532
EcoO65I GGTNACC 1 cut(s) 397
EcoRV GATATC 1 cut(s) 710
EcoT14I CCWWGG 1 cut(s) 994
EcoT38I GRGCYC 2 cut(s) 534, 638
ErhI CCWWGG 1 cut(s) 994
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 4 cut(s) 265, 412, 455, 717
FaqI GGGAC 2 cut(s) 354, 490
FatI CATG 4 cut(s) 261, 408, 451, 713
FauNDI CATATG 1 cut(s) 184
FbaI TGATCA 3 cut(s) 405, 975, 1093
FblI GTMKAC 1 cut(s) 895
Fnu4HI GCNGC 8 cut(s) 92, 171, 189, 216, 372, 579, 655, 840
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 2 cut(s) 534, 638
Fsp4HI GCNGC 8 cut(s) 92, 171, 189, 216, 372, 579, 655, 840
FspBI CTAG 5 cut(s) 114, 123, 443, 963, 995
GlaI GCGC 1 cut(s) 658
GluI GCNGC 8 cut(s) 92, 171, 189, 216, 372, 579, 655, 840
GsaI CCCAGC 1 cut(s) 635
HaeII RGCGCY 1 cut(s) 660
HaeIII GGCC 3 cut(s) 347, 413, 956
HapII CCGG 2 cut(s) 392, 618
HhaI GCGC 1 cut(s) 659
Hin1II CATG 4 cut(s) 265, 412, 455, 717
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 3 cut(s) 438, 546, 556
HindII GTYRAC 3 cut(s) 438, 546, 556
HindIII AAGCTT 2 cut(s) 467, 827
HinfI GANTC 4 cut(s) 221, 388, 757, 1015
HpaII CCGG 2 cut(s) 392, 618
HphI GGTGA 2 cut(s) 348, 579
Hpy166II GTNNAC 4 cut(s) 438, 546, 556, 896
Hpy188I TCNGA 2 cut(s) 177, 387
Hpy188III TCNNGA 3 cut(s) 225, 946, 1097
Hpy8I GTNNAC 4 cut(s) 438, 546, 556, 896
HpyAV CCTTC 5 cut(s) 14, 454, 523, 569, 649
HpyCH4III ACNGT 5 cut(s) 257, 481, 591, 685, 1091
HpyCH4IV ACGT 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 5 cut(s) 179, 188, 377, 461, 999
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 552
Hsp92II CATG 4 cut(s) 265, 412, 455, 717
HspAI GCGC 1 cut(s) 657
Ksp22I TGATCA 3 cut(s) 405, 975, 1093
Kzo9I GATC 6 cut(s) 311, 405, 504, 752, 975, 1093
LmnI GCTCC 2 cut(s) 641, 798
Lsp1109I GCAGC 7 cut(s) 103, 182, 200, 227, 383, 666, 826
MaeI CTAG 5 cut(s) 114, 123, 443, 963, 995
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 3 cut(s) 318, 397, 623
MalI GATC 6 cut(s) 313, 407, 506, 754, 977, 1095
MboI GATC 6 cut(s) 311, 405, 504, 752, 975, 1093
MboII GAAGA 3 cut(s) 508, 554, 852
MhlI GDGCHC 3 cut(s) 534, 638, 731
MlsI TGGCCA 2 cut(s) 413, 956
MluCI AATT 6 cut(s) 8, 325, 809, 852, 1020, 1081
MluNI TGGCCA 2 cut(s) 413, 956
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 2 cut(s) 408, 797
MnlI CCTC 9 cut(s) 245, 280, 297, 331, 560, 783, 899, 953, 1004
Mox20I TGGCCA 2 cut(s) 413, 956
MscI TGGCCA 2 cut(s) 413, 956
MseI TTAA 4 cut(s) 143, 350, 989, 1077
Msp20I TGGCCA 2 cut(s) 413, 956
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 2 cut(s) 392, 618
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 5 cut(s) 179, 188, 377, 461, 999
NdeI CATATG 1 cut(s) 184
NdeII GATC 6 cut(s) 311, 405, 504, 752, 975, 1093
NlaIII CATG 4 cut(s) 265, 412, 455, 717
NlaIV GGNNCC 3 cut(s) 582, 637, 800
NmeAIII GCCGAG 2 cut(s) 623, 630
NmuCI GTSAC 2 cut(s) 318, 623
PaeR7I CTCGAG 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 657
PfeI GAWTC 3 cut(s) 388, 757, 1015
PinAI ACCGGT 1 cut(s) 617
PkrI GCNGC 8 cut(s) 93, 172, 190, 217, 373, 580, 656, 841
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
PshBI ATTAAT 2 cut(s) 143, 1077
Psp124BI GAGCTC 1 cut(s) 534
PspEI GGTNACC 1 cut(s) 397
PspFI CCCAGC 1 cut(s) 631
PspN4I GGNNCC 3 cut(s) 582, 637, 800
PspPI GGNCC 2 cut(s) 524, 908
PspXI VCTCGAGB 1 cut(s) 724
PstNI CAGNNNCTG 2 cut(s) 224, 377
PvuII CAGCTG 1 cut(s) 839
SacI GAGCTC 1 cut(s) 534
SaqAI TTAA 4 cut(s) 143, 350, 989, 1077
SatI GCNGC 8 cut(s) 92, 171, 189, 216, 372, 579, 655, 840
Sau3AI GATC 6 cut(s) 311, 405, 504, 752, 975, 1093
Sau96I GGNCC 2 cut(s) 524, 908
SchI GAGTC 1 cut(s) 215
SduI GDGCHC 3 cut(s) 534, 638, 731
Sfr274I CTCGAG 1 cut(s) 724
SinI GGWCC 2 cut(s) 524, 908
SlaI CTCGAG 1 cut(s) 724
SmlI CTYRAG 1 cut(s) 724
SmoI CTYRAG 1 cut(s) 724
Sse9I AATT 6 cut(s) 8, 325, 809, 852, 1020, 1081
SsiI CCGC 3 cut(s) 395, 578, 935
SspI AATATT 1 cut(s) 241
SspMI CTAG 5 cut(s) 114, 123, 443, 963, 995
SstI GAGCTC 1 cut(s) 534
StyI CCWWGG 1 cut(s) 994
TaaI ACNGT 5 cut(s) 257, 481, 591, 685, 1091
TaiI ACGT 1 cut(s) 555
TaqI TCGA 5 cut(s) 303, 565, 725, 755, 1073
TaqII GACCGA 1 cut(s) 586
TasI AATT 6 cut(s) 8, 325, 809, 852, 1020, 1081
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 3 cut(s) 388, 757, 1015
Tru1I TTAA 4 cut(s) 143, 350, 989, 1077
Tru9I TTAA 4 cut(s) 143, 350, 989, 1077
TscAI CASTG 6 cut(s) 159, 382, 486, 750, 809, 1096
TseFI GTSAC 2 cut(s) 318, 623
TseI GCWGC 7 cut(s) 91, 170, 188, 215, 371, 654, 839
Tsp45I GTSAC 2 cut(s) 318, 623
TspDTI ATGAA 6 cut(s) 17, 161, 245, 322, 996, 1082
TspRI CASTG 6 cut(s) 159, 382, 486, 750, 809, 1096
VpaK11BI GGWCC 2 cut(s) 524, 908
VspI ATTAAT 2 cut(s) 143, 1077
XapI RAATTY 2 cut(s) 8, 325
XhoI CTCGAG 1 cut(s) 724
XmaJI CCTAGG 1 cut(s) 994
XmiI GTMKAC 1 cut(s) 895
XspI CTAG 5 cut(s) 114, 123, 443, 963, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.