Rroxscaffold_4G00316910

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000337
Physical Location & Seq
Reverse (-)
41875813 .. 41877318
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00316910.1

Sequence Viewer

Length: 891 bp
ATGGGGAAGCAGAGTAGTGTAGTTGATGGCTTAATAAAGCCAGTGATAACGATGGTGATCGCTCAGATTGCATATGCAGCAACGAGTATATTCTACAAAGTTGCAGCAGACTCTGGAATGAATCTCAGGATTCTCGTTGCCTACCGTCTCATGTTTTCATCTGCCATTATCGTTCCACTTGCTCTTAGACTCGAACGGAACAGCAGGCCCAAACTCACTCTAGTCGTACTCTTTCAAGCTTTTCTCTGCGGATTATTTGGGGGATCATTGTCAGAAAATTTATTCATAGAGGGATTAGTCTTAACATCACCAACCTTTGTTGCAGCCACTGCAAATCTGATTCCAGCCGTCACCTTCATCATGGCCATCTCTTTCAGGTTGGAGAAGTTGGCACTAGGAAGCCATGCGGGGAAGGCAAAGCTAGTGGGGACAGTGGTGGGTATGGGTGGTGCTATGATCTTTACCTTCTTCAAAGGACCAGAGTTCTCCATCTGGTCAATTCACGTTGACCTTCTTCGAGGACATCAGGCGGCAACATCACCGTCATCGTCCTCCTCCCACAGAAGCACCGGTAGTCACCTGCTGGGCTCCTTCTTGGCACTCGGCTCCGTCGTTTCCTATGTAATGTGGCTCATAGCTCAGGCAAAAATGAGCAAGAGATATCCATGCCATTACTCGAGCACGGCTCTATTGTCGGTAATGGGATCGATTCAGTCTGTAGCTTTTGCTCTCTGCGTGGAGAAGGATTGGAGCCAGTGGAAATTGGGTTGGAACATCAAGCTTTTTACAGCTGCATATGGAGGAGATTATTCAAGGGAAATCAAACAGCAATGCATTTTTCCAAAGTGGGACAGTGGCTGTGCAGGGAAGAGAATTTTCAGTCATACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

296

Amino Acids

32.09

Weight (kDa)

9.75

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 15 - 154 1.5e-10 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 588
Acc36I ACCTGC 1 cut(s) 588
AciI CCGC 3 cut(s) 249, 407, 530
AclWI GGATC 2 cut(s) 271, 712
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 2 cut(s) 277, 873
AfaI GTAC 1 cut(s) 228
AgeI ACCGGT 1 cut(s) 569
AgsI TTSAA 3 cut(s) 236, 472, 813
AluBI AGCT 6 cut(s) 239, 421, 638, 722, 781, 791
AluI AGCT 6 cut(s) 239, 421, 638, 722, 781, 791
Alw21I GWGCWC 1 cut(s) 683
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 2 cut(s) 271, 712
AlwNI CAGNNNCTG 3 cut(s) 113, 329, 858
Ama87I CYCGRG 1 cut(s) 676
AoxI GGCC 2 cut(s) 206, 363
ApeKI GCWGC 4 cut(s) 77, 104, 323, 791
ApoI RAATTY 2 cut(s) 277, 873
AsiGI ACCGGT 1 cut(s) 569
AspS9I GGNCC 2 cut(s) 207, 476
AsuHPI GGTGA 5 cut(s) 67, 300, 343, 531, 569
AvaI CYCGRG 1 cut(s) 676
AvaII GGWCC 1 cut(s) 476
BalI TGGCCA 1 cut(s) 365
BanII GRGCYC 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 683
BbvI GCAGC 4 cut(s) 89, 116, 335, 778
BccI CCATC 4 cut(s) 20, 46, 374, 497
BceAI ACGGC 2 cut(s) 332, 699
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 3 cut(s) 221, 395, 422
BfmI CTRYAG 1 cut(s) 717
BfuAI ACCTGC 1 cut(s) 588
BisI GCNGC 5 cut(s) 78, 105, 324, 531, 792
BlsI GCNGC 5 cut(s) 79, 106, 325, 532, 793
Bme18I GGWCC 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 676
BmgT120I GGNCC 2 cut(s) 207, 476
BmiI GGNNCC 3 cut(s) 589, 607, 752
BplI GAGNNNNNCTC 2 cut(s) 670, 702
Bpu10I CCTNAGC 1 cut(s) 639
Bsa29I ATCGAT 1 cut(s) 707
BsaBI GATNNNNATC 1 cut(s) 56
BsaWI WCCGGW 1 cut(s) 569
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 2 cut(s) 41, 754
Bse3DI GCAATG 1 cut(s) 836
Bse8I GATNNNNATC 1 cut(s) 56
BseCI ATCGAT 1 cut(s) 707
BseJI GATNNNNATC 1 cut(s) 56
BseMI GCAATG 1 cut(s) 836
BseMII CTCAG 3 cut(s) 77, 139, 653
BseNI ACTGG 2 cut(s) 41, 754
BseRI GAGGAG 2 cut(s) 544, 816
BseXI GCAGC 4 cut(s) 89, 116, 335, 778
BseYI CCCAGC 1 cut(s) 583
BsgI GTGCAG 1 cut(s) 882
BshFI GGCC 2 cut(s) 208, 365
BshTI ACCGGT 1 cut(s) 569
BshVI ATCGAT 1 cut(s) 707
BsiHKAI GWGCWC 1 cut(s) 683
BsiHKCI CYCGRG 1 cut(s) 676
BsiSI CCGG 1 cut(s) 570
BslFI GGGAC 2 cut(s) 442, 863
BsmAI GTCTC 1 cut(s) 152
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 442, 863
BsnI GGCC 2 cut(s) 208, 365
BsoBI CYCGRG 1 cut(s) 676
Bsp1286I GDGCHC 2 cut(s) 590, 683
Bsp143I GATC 4 cut(s) 57, 263, 456, 704
BspACI CCGC 3 cut(s) 249, 407, 530
BspANI GGCC 2 cut(s) 208, 365
BspCNI CTCAG 3 cut(s) 76, 138, 652
BspDI ATCGAT 1 cut(s) 707
BspLI GGNNCC 3 cut(s) 589, 607, 752
BspMI ACCTGC 1 cut(s) 588
BspPI GGATC 2 cut(s) 271, 712
BsrDI GCAATG 1 cut(s) 836
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 2 cut(s) 41, 754
BssAI RCCGGY 1 cut(s) 569
BssMI GATC 4 cut(s) 57, 263, 456, 704
Bst4CI ACNGT 4 cut(s) 146, 433, 543, 854
Bst6I CTCTTC 1 cut(s) 863
BstAPI GCANNNNNTGC 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 206
BstDEI CTNAG 4 cut(s) 63, 125, 185, 639
BstKTI GATC 4 cut(s) 60, 266, 459, 707
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 4 cut(s) 57, 263, 456, 704
BstMWI GCNNNNNNNGC 4 cut(s) 68, 77, 329, 413
BstSFI CTRYAG 1 cut(s) 717
BstV1I GCAGC 4 cut(s) 89, 116, 335, 778
Bsu15I ATCGAT 1 cut(s) 707
BsuRI GGCC 2 cut(s) 208, 365
BsuTUI ATCGAT 1 cut(s) 707
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 5 cut(s) 48, 327, 438, 761, 859
BveI ACCTGC 1 cut(s) 588
Cac8I GCNNGC 1 cut(s) 206
CaiI CAGNNNCTG 3 cut(s) 113, 329, 858
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 2 cut(s) 207, 476
ClaI ATCGAT 1 cut(s) 707
Csp6I GTAC 1 cut(s) 227
CspAI ACCGGT 1 cut(s) 569
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 4 cut(s) 151, 361, 404, 666
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 4 cut(s) 63, 125, 185, 639
DpnI GATC 4 cut(s) 59, 265, 458, 706
DpnII GATC 4 cut(s) 57, 263, 456, 704
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
Eco24I GRGCYC 1 cut(s) 590
Eco32I GATATC 1 cut(s) 662
Eco47I GGWCC 1 cut(s) 476
Eco88I CYCGRG 1 cut(s) 676
EcoRV GATATC 1 cut(s) 662
EcoT22I ATGCAT 1 cut(s) 836
EcoT38I GRGCYC 1 cut(s) 590
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 4 cut(s) 154, 364, 407, 669
FaqI GGGAC 2 cut(s) 442, 863
FatI CATG 4 cut(s) 150, 360, 403, 665
FauI CCCGC 1 cut(s) 400
FauNDI CATATG 2 cut(s) 73, 796
Fnu4HI GCNGC 5 cut(s) 78, 105, 324, 531, 792
FriOI GRGCYC 1 cut(s) 590
Fsp4HI GCNGC 5 cut(s) 78, 105, 324, 531, 792
FspBI CTAG 3 cut(s) 221, 395, 422
GluI GCNGC 5 cut(s) 78, 105, 324, 531, 792
GsaI CCCAGC 1 cut(s) 587
HaeIII GGCC 2 cut(s) 208, 365
HapII CCGG 1 cut(s) 570
Hin1II CATG 4 cut(s) 154, 364, 407, 669
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 2 cut(s) 237, 779
HinfI GANTC 6 cut(s) 110, 121, 130, 189, 340, 709
HpaII CCGG 1 cut(s) 570
HphI GGTGA 5 cut(s) 67, 300, 343, 531, 569
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 3 cut(s) 66, 274, 339
Hpy188III TCNNGA 2 cut(s) 114, 127
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 614
HpyAV CCTTC 6 cut(s) 364, 406, 475, 521, 601, 736
HpyCH4III ACNGT 4 cut(s) 146, 433, 543, 854
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 8 cut(s) 71, 77, 104, 323, 332, 794, 834, 863
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 77, 329, 413
HpyF3I CTNAG 4 cut(s) 63, 125, 185, 639
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 4 cut(s) 154, 364, 407, 669
Kzo9I GATC 4 cut(s) 57, 263, 456, 704
LmnI GCTCC 3 cut(s) 593, 611, 750
Lsp1109I GCAGC 4 cut(s) 89, 116, 335, 778
MaeI CTAG 3 cut(s) 221, 395, 422
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 2 cut(s) 349, 575
MalI GATC 4 cut(s) 59, 265, 458, 706
MboI GATC 4 cut(s) 57, 263, 456, 704
MboII GAAGA 3 cut(s) 460, 506, 880
MhlI GDGCHC 2 cut(s) 590, 683
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 4 cut(s) 277, 498, 761, 873
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 2 cut(s) 104, 183
MmeI TCCRAC 2 cut(s) 360, 749
MnlI CCTC 5 cut(s) 283, 512, 562, 565, 794
Mox20I TGGCCA 1 cut(s) 365
Mph1103I ATGCAT 1 cut(s) 836
MscI TGGCCA 1 cut(s) 365
MseI TTAA 2 cut(s) 32, 302
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 1 cut(s) 570
MwoI GCNNNNNNNGC 4 cut(s) 68, 77, 329, 413
NdeI CATATG 2 cut(s) 73, 796
NdeII GATC 4 cut(s) 57, 263, 456, 704
NlaIII CATG 4 cut(s) 154, 364, 407, 669
NlaIV GGNNCC 3 cut(s) 589, 607, 752
NmeAIII GCCGAG 1 cut(s) 582
NmuCI GTSAC 2 cut(s) 349, 575
NsiI ATGCAT 1 cut(s) 836
PaeR7I CTCGAG 1 cut(s) 676
PaqCI CACCTGC 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PfeI GAWTC 4 cut(s) 121, 130, 340, 709
PinAI ACCGGT 1 cut(s) 569
PkrI GCNGC 5 cut(s) 79, 106, 325, 532, 793
PleI GAGTC 2 cut(s) 104, 183
PpsI GAGTC 2 cut(s) 104, 183
PspFI CCCAGC 1 cut(s) 583
PspN4I GGNNCC 3 cut(s) 589, 607, 752
PspPI GGNCC 2 cut(s) 207, 476
PspXI VCTCGAGB 1 cut(s) 676
PstNI CAGNNNCTG 3 cut(s) 113, 329, 858
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 32, 302
SatI GCNGC 5 cut(s) 78, 105, 324, 531, 792
Sau3AI GATC 4 cut(s) 57, 263, 456, 704
Sau96I GGNCC 2 cut(s) 207, 476
SchI GAGTC 2 cut(s) 104, 183
SduI GDGCHC 2 cut(s) 590, 683
SfcI CTRYAG 1 cut(s) 717
Sfr274I CTCGAG 1 cut(s) 676
SinI GGWCC 1 cut(s) 476
SlaI CTCGAG 1 cut(s) 676
SmlI CTYRAG 1 cut(s) 676
SmoI CTYRAG 1 cut(s) 676
Sse9I AATT 4 cut(s) 277, 498, 761, 873
SsiI CCGC 3 cut(s) 249, 407, 530
SspMI CTAG 3 cut(s) 221, 395, 422
TaaI ACNGT 4 cut(s) 146, 433, 543, 854
TaiI ACGT 1 cut(s) 507
TaqI TCGA 4 cut(s) 192, 517, 677, 707
TasI AATT 4 cut(s) 277, 498, 761, 873
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 4 cut(s) 121, 130, 340, 709
Tru1I TTAA 2 cut(s) 32, 302
Tru9I TTAA 2 cut(s) 32, 302
TscAI CASTG 5 cut(s) 48, 334, 438, 761, 859
TseFI GTSAC 2 cut(s) 349, 575
TseI GCWGC 4 cut(s) 77, 104, 323, 791
Tsp45I GTSAC 2 cut(s) 349, 575
TspDTI ATGAA 4 cut(s) 134, 147, 274, 346
TspGWI ACGGA 2 cut(s) 211, 598
TspRI CASTG 5 cut(s) 48, 334, 438, 761, 859
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 2 cut(s) 277, 873
XhoI CTCGAG 1 cut(s) 676
XspI CTAG 3 cut(s) 221, 395, 422
Zsp2I ATGCAT 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.