WAT1-related protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 649 |
| AccII | CGCG | 1 cut(s) | 477 |
| AciI | CCGC | 2 cut(s) | 768, 1025 |
| AfaI | GTAC | 4 cut(s) | 13, 613, 756, 780 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 649, 667 |
| AflIII | ACRYGT | 1 cut(s) | 436 |
| AgsI | TTSAA | 6 cut(s) | 34, 191, 814, 1007, 1031, 1154 |
| AhlI | ACTAGT | 3 cut(s) | 14, 97, 1156 |
| AjnI | CCWGG | 1 cut(s) | 557 |
| AleI | CACNNNNGTG | 1 cut(s) | 370 |
| AluBI | AGCT | 3 cut(s) | 228, 927, 1150 |
| AluI | AGCT | 3 cut(s) | 228, 927, 1150 |
| Alw26I | GTCTC | 1 cut(s) | 900 |
| AoxI | GGCC | 2 cut(s) | 791, 823 |
| ApeKI | GCWGC | 7 cut(s) | 158, 264, 845, 924, 1058, 1079, 1097 |
| AspS9I | GGNCC | 1 cut(s) | 791 |
| AsuHPI | GGTGA | 3 cut(s) | 115, 277, 388 |
| BanII | GRGCYC | 1 cut(s) | 521 |
| BbvI | GCAGC | 7 cut(s) | 170, 276, 857, 936, 1066, 1070, 1084 |
| BccI | CCATC | 2 cut(s) | 134, 1111 |
| BceAI | ACGGC | 2 cut(s) | 81, 630 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1079, 1113 |
| BciT130I | CCWGG | 1 cut(s) | 559 |
| BcoDI | GTCTC | 1 cut(s) | 900 |
| BcuI | ACTAGT | 3 cut(s) | 14, 97, 1156 |
| BfaI | CTAG | 4 cut(s) | 15, 98, 842, 1157 |
| BfmI | CTRYAG | 3 cut(s) | 156, 262, 922 |
| BisI | GCNGC | 7 cut(s) | 159, 265, 846, 925, 1059, 1080, 1098 |
| BlpI | GCTNAGC | 1 cut(s) | 229 |
| BlsI | GCNGC | 7 cut(s) | 160, 266, 847, 926, 1060, 1081, 1099 |
| BmcAI | AGTACT | 1 cut(s) | 13 |
| Bme1390I | CCNGG | 1 cut(s) | 559 |
| BmgT120I | GGNCC | 1 cut(s) | 791 |
| BmiI | GGNNCC | 1 cut(s) | 520 |
| BmrFI | CCNGG | 1 cut(s) | 559 |
| BmsI | GCATC | 5 cut(s) | 39, 432, 563, 644, 1070 |
| BpmI | CTGGAG | 1 cut(s) | 1050 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 229 |
| BpuEI | CTTGAG | 1 cut(s) | 592 |
| Bsa29I | ATCGAT | 1 cut(s) | 1106 |
| BsaAI | YACGTR | 1 cut(s) | 452 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 649, 667 |
| Bse1I | ACTGG | 5 cut(s) | 38, 278, 643, 751, 1067 |
| Bse3DI | GCAATG | 1 cut(s) | 485 |
| BseBI | CCWGG | 1 cut(s) | 559 |
| BseCI | ATCGAT | 1 cut(s) | 1106 |
| BseGI | GGATG | 3 cut(s) | 84, 126, 554 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 649, 667 |
| BseMI | GCAATG | 1 cut(s) | 485 |
| BseNI | ACTGG | 5 cut(s) | 38, 278, 643, 751, 1067 |
| BseRI | GAGGAG | 1 cut(s) | 1107 |
| BseXI | GCAGC | 7 cut(s) | 170, 276, 857, 936, 1066, 1070, 1084 |
| BseYI | CCCAGC | 1 cut(s) | 699 |
| BsgI | GTGCAG | 1 cut(s) | 39 |
| Bsh1236I | CGCG | 1 cut(s) | 477 |
| BshFI | GGCC | 2 cut(s) | 793, 825 |
| BshVI | ATCGAT | 1 cut(s) | 1106 |
| BslFI | GGGAC | 4 cut(s) | 346, 376, 397, 1024 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 649, 667 |
| BsmAI | GTCTC | 1 cut(s) | 900 |
| BsmFI | GGGAC | 4 cut(s) | 346, 376, 397, 1024 |
| BsmI | GAATGC | 1 cut(s) | 789 |
| BsnI | GGCC | 2 cut(s) | 793, 825 |
| Bsp1286I | GDGCHC | 1 cut(s) | 521 |
| Bsp143I | GATC | 2 cut(s) | 1033, 1107 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 229 |
| BspACI | CCGC | 2 cut(s) | 768, 1025 |
| BspANI | GGCC | 2 cut(s) | 793, 825 |
| BspDI | ATCGAT | 1 cut(s) | 1106 |
| BspFNI | CGCG | 1 cut(s) | 477 |
| BspLI | GGNNCC | 1 cut(s) | 520 |
| BspMAI | CTGCAG | 3 cut(s) | 160, 266, 926 |
| BspQI | GCTCTTC | 1 cut(s) | 398 |
| BsrDI | GCAATG | 1 cut(s) | 485 |
| BsrI | ACTGG | 5 cut(s) | 38, 278, 643, 751, 1067 |
| BssMI | GATC | 2 cut(s) | 1033, 1107 |
| Bst2UI | CCWGG | 1 cut(s) | 559 |
| Bst4CI | ACNGT | 4 cut(s) | 143, 473, 798, 1076 |
| Bst6I | CTCTTC | 1 cut(s) | 398 |
| BstBAI | YACGTR | 1 cut(s) | 452 |
| BstC8I | GCNNGC | 1 cut(s) | 262 |
| BstDEI | CTNAG | 2 cut(s) | 229, 585 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 665 |
| BstF5I | GGATG | 3 cut(s) | 84, 126, 554 |
| BstFNI | CGCG | 1 cut(s) | 477 |
| BstKTI | GATC | 2 cut(s) | 1036, 1110 |
| BstMAI | GTCTC | 1 cut(s) | 900 |
| BstMBI | GATC | 2 cut(s) | 1033, 1107 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 845 |
| BstNI | CCWGG | 1 cut(s) | 559 |
| BstSCI | CCNGG | 1 cut(s) | 557 |
| BstSFI | CTRYAG | 3 cut(s) | 156, 262, 922 |
| BstSNI | TACGTA | 1 cut(s) | 452 |
| BstUI | CGCG | 1 cut(s) | 477 |
| BstV1I | GCAGC | 7 cut(s) | 170, 276, 857, 936, 1066, 1070, 1084 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1054 |
| Bsu15I | ATCGAT | 1 cut(s) | 1106 |
| BsuRI | GGCC | 2 cut(s) | 793, 825 |
| BsuTUI | ATCGAT | 1 cut(s) | 1106 |
| BtsCI | GGATG | 3 cut(s) | 84, 126, 554 |
| BtsI | GCAGTG | 2 cut(s) | 27, 919 |
| BtsIMutI | CAGTG | 6 cut(s) | 27, 45, 692, 794, 919, 1081 |
| Cac8I | GCNNGC | 1 cut(s) | 262 |
| Cfr13I | GGNCC | 1 cut(s) | 791 |
| ClaI | ATCGAT | 1 cut(s) | 1106 |
| Csp6I | GTAC | 4 cut(s) | 12, 612, 755, 779 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 339, 374 |
| CviAII | CATG | 5 cut(s) | 464, 631, 771, 964, 1054 |
| CviQI | GTAC | 4 cut(s) | 12, 612, 755, 779 |
| DdeI | CTNAG | 2 cut(s) | 229, 585 |
| DpnI | GATC | 2 cut(s) | 1035, 1109 |
| DpnII | GATC | 2 cut(s) | 1033, 1107 |
| Eam1104I | CTCTTC | 1 cut(s) | 398 |
| EarI | CTCTTC | 1 cut(s) | 398 |
| Eco105I | TACGTA | 1 cut(s) | 452 |
| Eco24I | GRGCYC | 1 cut(s) | 521 |
| Eco32I | GATATC | 2 cut(s) | 706, 1093 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 665 |
| EcoRII | CCWGG | 1 cut(s) | 557 |
| EcoRV | GATATC | 2 cut(s) | 706, 1093 |
| EcoT38I | GRGCYC | 1 cut(s) | 521 |
| FaeI | CATG | 5 cut(s) | 467, 634, 774, 967, 1057 |
| FaqI | GGGAC | 4 cut(s) | 346, 376, 397, 1024 |
| FatI | CATG | 5 cut(s) | 463, 630, 770, 963, 1053 |
| FauNDI | CATATG | 1 cut(s) | 70 |
| Fnu4HI | GCNGC | 7 cut(s) | 159, 265, 846, 925, 1059, 1080, 1098 |
| FokI | GGATG | 3 cut(s) | 91, 113, 541 |
| FriOI | GRGCYC | 1 cut(s) | 521 |
| Fsp4HI | GCNGC | 7 cut(s) | 159, 265, 846, 925, 1059, 1080, 1098 |
| FspBI | CTAG | 4 cut(s) | 15, 98, 842, 1157 |
| GluI | GCNGC | 7 cut(s) | 159, 265, 846, 925, 1059, 1080, 1098 |
| GsaI | CCCAGC | 1 cut(s) | 703 |
| GsuI | CTGGAG | 1 cut(s) | 1050 |
| HaeIII | GGCC | 2 cut(s) | 793, 825 |
| Hin1II | CATG | 5 cut(s) | 467, 634, 774, 967, 1057 |
| HincII | GTYRAC | 2 cut(s) | 432, 1072 |
| HindII | GTYRAC | 2 cut(s) | 432, 1072 |
| HinfI | GANTC | 6 cut(s) | 682, 735, 760, 940, 1003, 1131 |
| HpaI | GTTAAC | 1 cut(s) | 1072 |
| HphI | GGTGA | 3 cut(s) | 115, 277, 388 |
| Hpy166II | GTNNAC | 3 cut(s) | 432, 779, 1072 |
| Hpy188I | TCNGA | 4 cut(s) | 63, 573, 621, 1122 |
| Hpy188III | TCNNGA | 1 cut(s) | 732 |
| Hpy8I | GTNNAC | 3 cut(s) | 432, 779, 1072 |
| HpyAV | CCTTC | 4 cut(s) | 298, 409, 532, 862 |
| HpyCH4III | ACNGT | 4 cut(s) | 143, 473, 798, 1076 |
| HpyCH4IV | ACGT | 3 cut(s) | 248, 438, 451 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 845 |
| HpyF3I | CTNAG | 2 cut(s) | 229, 585 |
| HpySE526I | ACGT | 3 cut(s) | 248, 438, 451 |
| Hsp92II | CATG | 5 cut(s) | 467, 634, 774, 967, 1057 |
| KspAI | GTTAAC | 1 cut(s) | 1072 |
| Kzo9I | GATC | 2 cut(s) | 1033, 1107 |
| LguI | GCTCTTC | 1 cut(s) | 398 |
| LmnI | GCTCC | 2 cut(s) | 524, 1105 |
| Lsp1109I | GCAGC | 7 cut(s) | 170, 276, 857, 936, 1066, 1070, 1084 |
| LweI | GCATC | 5 cut(s) | 39, 432, 563, 644, 1070 |
| MaeI | CTAG | 4 cut(s) | 15, 98, 842, 1157 |
| MaeII | ACGT | 3 cut(s) | 248, 438, 451 |
| MaeIII | GTNAC | 4 cut(s) | 143, 452, 739, 1159 |
| MalI | GATC | 2 cut(s) | 1035, 1109 |
| MboI | GATC | 2 cut(s) | 1033, 1107 |
| MboII | GAAGA | 3 cut(s) | 385, 894, 1043 |
| MhlI | GDGCHC | 1 cut(s) | 521 |
| MluCI | AATT | 3 cut(s) | 312, 807, 945 |
| MlyI | GAGTC | 4 cut(s) | 691, 744, 754, 1125 |
| MmeI | TCCRAC | 1 cut(s) | 91 |
| MnlI | CCTC | 9 cut(s) | 100, 183, 312, 374, 556, 666, 782, 929, 1128 |
| MseI | TTAA | 5 cut(s) | 236, 441, 860, 890, 1071 |
| MslI | CAYNNNNRTG | 2 cut(s) | 370, 1052 |
| MspR9I | CCNGG | 1 cut(s) | 559 |
| Mva1269I | GAATGC | 1 cut(s) | 789 |
| MvaI | CCWGG | 1 cut(s) | 559 |
| MvnI | CGCG | 1 cut(s) | 477 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 845 |
| NdeI | CATATG | 1 cut(s) | 70 |
| NdeII | GATC | 2 cut(s) | 1033, 1107 |
| NlaIII | CATG | 5 cut(s) | 467, 634, 774, 967, 1057 |
| NlaIV | GGNNCC | 1 cut(s) | 520 |
| OliI | CACNNNNGTG | 1 cut(s) | 370 |
| PciSI | GCTCTTC | 1 cut(s) | 398 |
| PctI | GAATGC | 1 cut(s) | 789 |
| PfeI | GAWTC | 2 cut(s) | 940, 1003 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 649 |
| PkrI | GCNGC | 7 cut(s) | 160, 266, 847, 926, 1060, 1081, 1099 |
| PleI | GAGTC | 4 cut(s) | 690, 743, 754, 1125 |
| PpsI | GAGTC | 4 cut(s) | 690, 743, 754, 1125 |
| Ppu21I | YACGTR | 1 cut(s) | 452 |
| Psp6I | CCWGG | 1 cut(s) | 557 |
| PspFI | CCCAGC | 1 cut(s) | 699 |
| PspGI | CCWGG | 1 cut(s) | 557 |
| PspN4I | GGNNCC | 1 cut(s) | 520 |
| PspPI | GGNCC | 1 cut(s) | 791 |
| PstI | CTGCAG | 3 cut(s) | 160, 266, 926 |
| RsaI | GTAC | 4 cut(s) | 13, 613, 756, 780 |
| RsaNI | GTAC | 4 cut(s) | 12, 612, 755, 779 |
| RseI | CAYNNNNRTG | 2 cut(s) | 370, 1052 |
| SapI | GCTCTTC | 1 cut(s) | 398 |
| SaqAI | TTAA | 5 cut(s) | 236, 441, 860, 890, 1071 |
| SatI | GCNGC | 7 cut(s) | 159, 265, 846, 925, 1059, 1080, 1098 |
| Sau3AI | GATC | 2 cut(s) | 1033, 1107 |
| Sau96I | GGNCC | 1 cut(s) | 791 |
| ScaI | AGTACT | 1 cut(s) | 13 |
| SchI | GAGTC | 4 cut(s) | 691, 744, 754, 1125 |
| ScrFI | CCNGG | 1 cut(s) | 559 |
| SduI | GDGCHC | 1 cut(s) | 521 |
| SfaNI | GCATC | 5 cut(s) | 39, 432, 563, 644, 1070 |
| SfcI | CTRYAG | 3 cut(s) | 156, 262, 922 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 370, 1052 |
| SmlI | CTYRAG | 1 cut(s) | 607 |
| SmoI | CTYRAG | 1 cut(s) | 607 |
| SnaBI | TACGTA | 1 cut(s) | 452 |
| SpeI | ACTAGT | 3 cut(s) | 14, 97, 1156 |
| Sse9I | AATT | 3 cut(s) | 312, 807, 945 |
| SsiI | CCGC | 2 cut(s) | 768, 1025 |
| SspMI | CTAG | 4 cut(s) | 15, 98, 842, 1157 |
| StyD4I | CCNGG | 1 cut(s) | 557 |
| TaaI | ACNGT | 4 cut(s) | 143, 473, 798, 1076 |
| TaiI | ACGT | 3 cut(s) | 251, 441, 454 |
| TaqI | TCGA | 1 cut(s) | 1106 |
| TasI | AATT | 3 cut(s) | 312, 807, 945 |
| TatI | WGTACW | 1 cut(s) | 11 |
| TfiI | GAWTC | 2 cut(s) | 940, 1003 |
| Tru1I | TTAA | 5 cut(s) | 236, 441, 860, 890, 1071 |
| Tru9I | TTAA | 5 cut(s) | 236, 441, 860, 890, 1071 |
| TscAI | CASTG | 6 cut(s) | 27, 45, 692, 801, 926, 1081 |
| TseI | GCWGC | 7 cut(s) | 158, 264, 845, 924, 1058, 1079, 1097 |
| TspDTI | ATGAA | 6 cut(s) | 95, 280, 474, 480, 879, 1007 |
| TspRI | CASTG | 6 cut(s) | 27, 45, 692, 801, 926, 1081 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 649 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 665 |
| XspI | CTAG | 4 cut(s) | 15, 98, 842, 1157 |
| ZrmI | AGTACT | 1 cut(s) | 13 |