Rorug07G0224000

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000337
Physical Location & Seq
Forward (+)
20398523 .. 20399209
687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0224000.1

Sequence Viewer

Length: 687 bp
ATGGCCATGAGACAAATCCAAGTCGCCGACGACACGCTTGTAATCCATGAGCTCGAGGACGTTTGTGACTCGGCCACTGGCCGAGTACTCACCGGCTCATGGATTTGGGCCTCGGCTTTGGTCCTATCCAACTGGATGACCAACGAAAGCCGGACCAACTTCAGTTTTCTAGGCAAGACCGTGCTTGAGCTTGGTGCGGGAGCTGGACTCCCCGGCTTGACAGCAGCCCGGCTAGGAGCGAGCCAAGTTGTGCTCACCGACATCAAGCCGCTGCTTCCAGGTTTGCTGAAGAATGTCGAAGCCAACGGACTTGGTGAACTAGTCAATGTCAGGGAACTCATATGGGGATCAGACGAGTCATTGAGCGAAGTCGGTGAGTTTGACTTGGTTTTGATGTCAGATGTGTTCTACAACGCCGAGGAGATGGTTGGCTTGGCCAAGACGTTGAAATGTGTGTGCAACAGAAAGACGACAGTTTTGGCAGCAACTGAGGTAAGGCCCTCAACAGCCGAGTGTCTGAGCGAGTTTATTAGAGAAGGCTTTATGCTTGTTGAGTTGCCGAGTGAGTTCGCTTCTTCAGAAGGAATGGACATGGAGAGTTTGGATTCCTTTGCCATCTTCCAAGTCATACCACCGCATGAAGAAAGCCAGATGGATGGCCCTTGTAATCGAGACCACAGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

228

Amino Acids

24.84

Weight (kDa)

4.42

Isoelectric Point (pI)

30.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 31 - 169 1.7e-28 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 197, 269, 635
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 4 cut(s) 3, 72, 79, 435
AcuI CTGAAG 3 cut(s) 145, 308, 561
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 1 cut(s) 99
AgsI TTSAA 1 cut(s) 448
AhlI ACTAGT 1 cut(s) 319
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 3 cut(s) 52, 190, 203
AluI AGCT 3 cut(s) 52, 190, 203
Alw21I GWGCWC 2 cut(s) 54, 255
Alw26I GTCTC 2 cut(s) 4, 666
AlwI GGATC 1 cut(s) 355
AlwNI CAGNNNCTG 1 cut(s) 488
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 7 cut(s) 3, 72, 79, 108, 435, 497, 658
ApeKI GCWGC 3 cut(s) 224, 271, 482
ArsI GACNNNNNNTTYG 2 cut(s) 460, 492
AspS9I GGNCC 5 cut(s) 108, 121, 153, 498, 659
AsuC2I CCSGG 2 cut(s) 213, 229
AsuHPI GGTGA 4 cut(s) 82, 247, 326, 386
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 2 cut(s) 121, 153
BalI TGGCCA 2 cut(s) 5, 437
BanII GRGCYC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 54, 255
BbvI GCAGC 3 cut(s) 236, 258, 494
BccI CCATC 4 cut(s) 418, 623, 646, 650
BciT130I CCWGG 1 cut(s) 279
BcnI CCSGG 2 cut(s) 213, 229
BcoDI GTCTC 2 cut(s) 4, 666
BcuI ACTAGT 1 cut(s) 319
BfaI CTAG 3 cut(s) 170, 233, 320
BisI GCNGC 4 cut(s) 225, 269, 272, 483
BlsI GCNGC 4 cut(s) 226, 270, 273, 484
BmcAI AGTACT 1 cut(s) 87
Bme1390I CCNGG 3 cut(s) 213, 229, 279
Bme18I GGWCC 2 cut(s) 121, 153
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 5 cut(s) 108, 121, 153, 498, 659
BmrFI CCNGG 3 cut(s) 213, 229, 279
BplI GAGNNNNNCTC 2 cut(s) 192, 224
BpuEI CTTGAG 1 cut(s) 206
BpuMI CCSGG 2 cut(s) 213, 229
BsaI GGTCTC 1 cut(s) 666
BsaJI CCNNGG 3 cut(s) 111, 211, 417
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 82, 137
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 3 cut(s) 111, 211, 417
BseGI GGATG 2 cut(s) 141, 661
BseLI CCNNNNNNNGG 1 cut(s) 99
BseMII CTCAG 2 cut(s) 480, 509
BseNI ACTGG 2 cut(s) 82, 137
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 3 cut(s) 236, 258, 494
BshFI GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 660
BsiHKAI GWGCWC 2 cut(s) 54, 255
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 4 cut(s) 93, 151, 213, 229
BslI CCNNNNNNNGG 1 cut(s) 99
BsmAI GTCTC 2 cut(s) 4, 666
BsnI GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 660
Bso31I GGTCTC 1 cut(s) 666
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 2 cut(s) 54, 255
Bsp143I GATC 1 cut(s) 347
BspACI CCGC 3 cut(s) 197, 269, 635
BspANI GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 660
BspCNI CTCAG 2 cut(s) 481, 510
BspPI GGATC 1 cut(s) 355
BspTNI GGTCTC 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 82, 137
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 3 cut(s) 111, 211, 417
BssMI GATC 1 cut(s) 347
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 2 cut(s) 181, 475
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 2 cut(s) 489, 518
BstF5I GGATG 2 cut(s) 141, 661
BstKTI GATC 1 cut(s) 350
BstMAI GTCTC 2 cut(s) 4, 666
BstMBI GATC 1 cut(s) 347
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 3 cut(s) 211, 227, 277
BstV1I GCAGC 3 cut(s) 236, 258, 494
BstXI CCANNNNNNTGG 1 cut(s) 656
BsuRI GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 660
BtsCI GGATG 2 cut(s) 141, 661
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 241
CaiI CAGNNNCTG 1 cut(s) 488
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 5 cut(s) 108, 121, 153, 498, 659
Csp6I GTAC 1 cut(s) 86
CviAII CATG 6 cut(s) 7, 47, 99, 592, 638, 684
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 2 cut(s) 489, 518
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
EaeI YGGCCR 4 cut(s) 3, 72, 79, 435
Ecl136II GAGCTC 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 666
Eco47I GGWCC 2 cut(s) 121, 153
Eco53kI GAGCTC 1 cut(s) 52
Eco57I CTGAAG 3 cut(s) 145, 308, 561
Eco88I CYCGRG 1 cut(s) 53
EcoICRI GAGCTC 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 498
EcoRII CCWGG 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 54
FaeI CATG 6 cut(s) 10, 50, 102, 595, 641, 687
FatI CATG 6 cut(s) 6, 46, 98, 591, 637, 683
FauI CCCGC 1 cut(s) 190
FauNDI CATATG 1 cut(s) 341
Fnu4HI GCNGC 4 cut(s) 225, 269, 272, 483
FokI GGATG 2 cut(s) 148, 668
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 4 cut(s) 225, 269, 272, 483
FspBI CTAG 3 cut(s) 170, 233, 320
GluI GCNGC 4 cut(s) 225, 269, 272, 483
HaeIII GGCC 7 cut(s) 5, 74, 81, 110, 437, 499, 660
HapII CCGG 4 cut(s) 93, 151, 213, 229
Hin1II CATG 6 cut(s) 10, 50, 102, 595, 641, 687
HinfI GANTC 4 cut(s) 68, 207, 356, 605
HpaII CCGG 4 cut(s) 93, 151, 213, 229
HphI GGTGA 4 cut(s) 82, 247, 326, 386
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 4 cut(s) 352, 400, 519, 580
Hpy188III TCNNGA 1 cut(s) 671
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 530, 575
HpyCH4III ACNGT 2 cut(s) 181, 475
HpyCH4IV ACGT 2 cut(s) 60, 443
HpyCH4V TGCA 1 cut(s) 459
HpyF3I CTNAG 2 cut(s) 489, 518
HpySE526I ACGT 2 cut(s) 60, 443
Hsp92II CATG 6 cut(s) 10, 50, 102, 595, 641, 687
Kzo9I GATC 1 cut(s) 347
LmnI GCTCC 2 cut(s) 200, 236
Lsp1109I GCAGC 3 cut(s) 236, 258, 494
MaeI CTAG 3 cut(s) 170, 233, 320
MaeII ACGT 2 cut(s) 60, 443
MaeIII GTNAC 1 cut(s) 65
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 4 cut(s) 301, 567, 610, 653
MhlI GDGCHC 2 cut(s) 54, 255
MlsI TGGCCA 2 cut(s) 5, 437
MluNI TGGCCA 2 cut(s) 5, 437
MlyI GAGTC 3 cut(s) 62, 201, 365
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 5 cut(s) 49, 121, 412, 484, 511
Mox20I TGGCCA 2 cut(s) 5, 437
MscI TGGCCA 2 cut(s) 5, 437
Msp20I TGGCCA 2 cut(s) 5, 437
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 4 cut(s) 93, 151, 213, 229
MspR9I CCNGG 3 cut(s) 213, 229, 279
MvaI CCWGG 1 cut(s) 279
NciI CCSGG 2 cut(s) 213, 229
NdeI CATATG 1 cut(s) 341
NdeII GATC 1 cut(s) 347
NlaIII CATG 6 cut(s) 10, 50, 102, 595, 641, 687
NmeAIII GCCGAG 6 cut(s) 50, 92, 107, 442, 535, 585
NmuCI GTSAC 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 53
PfeI GAWTC 1 cut(s) 605
PkrI GCNGC 4 cut(s) 226, 270, 273, 484
PleI GAGTC 3 cut(s) 62, 201, 364
PpsI GAGTC 3 cut(s) 62, 201, 364
Psp124BI GAGCTC 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspPI GGNCC 5 cut(s) 108, 121, 153, 498, 659
PspXI VCTCGAGB 1 cut(s) 53
PstNI CAGNNNCTG 1 cut(s) 488
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SacI GAGCTC 1 cut(s) 54
SatI GCNGC 4 cut(s) 225, 269, 272, 483
Sau3AI GATC 1 cut(s) 347
Sau96I GGNCC 5 cut(s) 108, 121, 153, 498, 659
ScaI AGTACT 1 cut(s) 87
SchI GAGTC 3 cut(s) 62, 201, 365
ScrFI CCNGG 3 cut(s) 213, 229, 279
SduI GDGCHC 2 cut(s) 54, 255
SetI ASST 7 cut(s) 54, 63, 192, 205, 283, 446, 495
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 2 cut(s) 121, 153
SlaI CTCGAG 1 cut(s) 53
SmlI CTYRAG 2 cut(s) 53, 185
SmoI CTYRAG 2 cut(s) 53, 185
SpeI ACTAGT 1 cut(s) 319
SsiI CCGC 3 cut(s) 197, 269, 635
SspMI CTAG 3 cut(s) 170, 233, 320
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 3 cut(s) 211, 227, 277
TaaI ACNGT 2 cut(s) 181, 475
TaiI ACGT 2 cut(s) 63, 446
TaqI TCGA 3 cut(s) 54, 297, 670
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 1 cut(s) 605
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 3 cut(s) 224, 271, 482
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 654
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 1 cut(s) 82
VpaK11BI GGWCC 2 cut(s) 121, 153
XhoI CTCGAG 1 cut(s) 53
XspI CTAG 3 cut(s) 170, 233, 320
ZrmI AGTACT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.