Rorug07G0224500

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000337
Physical Location & Seq
Forward (+)
20491528 .. 20492406
879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0224500.1

Sequence Viewer

Length: 675 bp
ATGAATTTCATATTAGAACTACATATATTCCTCTACATGAGCAAGAAACCAGAGGAAACAATGCACCATATTTCTTTCTTTTCAATCTCTTTATGCTGCTGCTTATTTTTCCTTATAATTCCTCATGAAAATTTCTCACCAATTACTGCAATATCTGTTGATATAATCAACCAAACCTGCAGAAGATGTGCCGATGAATCCCACGTCTTTAGCTATGACCTATGCACAACCTCTCTCCAAGCAGTTCCGGTCAGCCATGTGGCTAATCTCCAAGGACTAGCATTGATAGCAATGGAGCTAGCACTAAACAATGCAAGCAACACGCTTCAGATCATAAAAAATTATTTGTTGATGAGCAACGAAAGTCTAGATCCTTTTGTTGTGGTTTGCTTGAAGGACTGCTTGCAGCTCTATTCAGACGGGATCACAACACTGGAGGATGCTGAAGGAGCATTTCTGGGTGAGCATTATGACAACGCCAATATTTGTCTTAGTGCAGTTACGGAAGCATCGAGTACATGTGAGGAAGGCTTTAAGGAGAAGGAAGGTGAAGTATTACTGTCTCCATTGACAAATGAGAATTACAATCTTTTTCAATTATGTGATATTGCACTGTGCATAGGCCACTTGCTTACCTTGGGTTCTACCTTCTTGATGAGGTCCTCTGCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

224

Amino Acids

24.87

Weight (kDa)

4.58

Isoelectric Point (pI)

53.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 55 - 207 2.4e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 116
Acc36I ACCTGC 1 cut(s) 185
AclWI GGATC 2 cut(s) 365, 431
AcsI RAATTY 2 cut(s) 4, 130
AcuI CTGAAG 2 cut(s) 311, 465
AfaI GTAC 1 cut(s) 517
AflIII ACRYGT 1 cut(s) 518
AgsI TTSAA 3 cut(s) 84, 394, 596
AjiI CACGTC 1 cut(s) 205
AluBI AGCT 3 cut(s) 213, 298, 409
AluI AGCT 3 cut(s) 213, 298, 409
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 2 cut(s) 365, 431
AoxI GGCC 1 cut(s) 622
ApeKI GCWGC 3 cut(s) 96, 99, 406
ApoI RAATTY 2 cut(s) 4, 130
AspS9I GGNCC 1 cut(s) 660
AsuHPI GGTGA 3 cut(s) 129, 473, 560
AsuNHI GCTAGC 1 cut(s) 298
AvaII GGWCC 1 cut(s) 660
BbvI GCAGC 3 cut(s) 83, 86, 418
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 3 cut(s) 278, 299, 368
BfmI CTRYAG 1 cut(s) 178
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 3 cut(s) 97, 100, 407
BlsI GCNGC 3 cut(s) 98, 101, 408
Bme18I GGWCC 1 cut(s) 660
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 660
BmsI GCATC 2 cut(s) 430, 518
BmtI GCTAGC 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 455
BsaJI CCNNGG 2 cut(s) 271, 636
BsaWI WCCGGW 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 271, 636
BseGI GGATG 1 cut(s) 445
BseMI GCAATG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 3 cut(s) 83, 86, 418
BsgI GTGCAG 1 cut(s) 516
BshFI GGCC 1 cut(s) 624
BsiSI CCGG 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 567
BsnI GGCC 1 cut(s) 624
Bsp143I GATC 3 cut(s) 330, 370, 423
BspANI GGCC 1 cut(s) 624
BspHI TCATGA 1 cut(s) 124
BspMAI CTGCAG 1 cut(s) 182
BspMI ACCTGC 1 cut(s) 185
BspOI GCTAGC 1 cut(s) 302
BspPI GGATC 2 cut(s) 365, 431
BsrDI GCAATG 1 cut(s) 297
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 2 cut(s) 271, 636
BssMI GATC 3 cut(s) 330, 370, 423
BssT1I CCWWGG 2 cut(s) 271, 636
Bst4CI ACNGT 2 cut(s) 561, 615
BstC8I GCNNGC 3 cut(s) 300, 316, 404
BstDEI CTNAG 1 cut(s) 491
BstF5I GGATG 1 cut(s) 445
BstKTI GATC 3 cut(s) 333, 373, 426
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 3 cut(s) 330, 370, 423
BstMWI GCNNNNNNNGC 2 cut(s) 287, 449
BstNSI RCATGY 1 cut(s) 522
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 3 cut(s) 83, 86, 418
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 1 cut(s) 624
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 1 cut(s) 445
BtsIMutI CAGTG 2 cut(s) 431, 611
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 300, 316, 404
CciI TCATGA 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 660
Csp6I GTAC 1 cut(s) 516
CviAII CATG 4 cut(s) 37, 125, 257, 519
CviJI RGCY 7 cut(s) 213, 255, 263, 298, 409, 531, 624
CviKI_1 RGCY 7 cut(s) 213, 255, 263, 298, 409, 531, 624
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 1 cut(s) 491
DpnI GATC 3 cut(s) 332, 372, 425
DpnII GATC 3 cut(s) 330, 370, 423
Eco130I CCWWGG 2 cut(s) 271, 636
Eco47I GGWCC 1 cut(s) 660
Eco57I CTGAAG 2 cut(s) 311, 465
EcoO109I RGGNCCY 1 cut(s) 660
EcoT14I CCWWGG 2 cut(s) 271, 636
ErhI CCWWGG 2 cut(s) 271, 636
FaeI CATG 4 cut(s) 40, 128, 260, 522
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FatI CATG 4 cut(s) 36, 124, 256, 518
Fnu4HI GCNGC 3 cut(s) 97, 100, 407
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 3 cut(s) 97, 100, 407
FspBI CTAG 3 cut(s) 278, 299, 368
GluI GCNGC 3 cut(s) 97, 100, 407
GsuI CTGGAG 1 cut(s) 455
HaeIII GGCC 1 cut(s) 624
HapII CCGG 1 cut(s) 248
Hin1II CATG 4 cut(s) 40, 128, 260, 522
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 248
HphI GGTGA 3 cut(s) 129, 473, 560
Hpy188I TCNGA 2 cut(s) 330, 418
Hpy188III TCNNGA 3 cut(s) 125, 368, 652
HpyAV CCTTC 6 cut(s) 388, 440, 521, 535, 539, 658
HpyCH4III ACNGT 2 cut(s) 561, 615
HpyCH4IV ACGT 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 449
HpyF3I CTNAG 1 cut(s) 491
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 4 cut(s) 40, 128, 260, 522
Kzo9I GATC 3 cut(s) 330, 370, 423
LmnI GCTCC 2 cut(s) 295, 449
LpnPI CCDG 5 cut(s) 63, 190, 261, 419, 443
Lsp1109I GCAGC 3 cut(s) 83, 86, 418
LweI GCATC 2 cut(s) 430, 518
MaeI CTAG 3 cut(s) 278, 299, 368
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 499
MalI GATC 3 cut(s) 332, 372, 425
MboI GATC 3 cut(s) 330, 370, 423
MboII GAAGA 1 cut(s) 195
MflI RGATCY 1 cut(s) 370
MluCI AATT 7 cut(s) 4, 117, 130, 141, 340, 580, 596
MnlI CCTC 8 cut(s) 41, 46, 132, 241, 430, 517, 651, 673
MseI TTAA 2 cut(s) 534, 673
MspI CCGG 1 cut(s) 248
MwoI GCNNNNNNNGC 2 cut(s) 287, 449
NdeII GATC 3 cut(s) 330, 370, 423
NheI GCTAGC 1 cut(s) 298
NlaIII CATG 4 cut(s) 40, 128, 260, 522
NspI RCATGY 1 cut(s) 522
PagI TCATGA 1 cut(s) 124
PciI ACATGT 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 509
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 3 cut(s) 98, 101, 408
PpuMI RGGWCCY 1 cut(s) 660
PscI ACATGT 1 cut(s) 518
PsiI TTATAA 1 cut(s) 116
Psp5II RGGWCCY 1 cut(s) 660
PspPI GGNCC 1 cut(s) 660
PspPPI RGGWCCY 1 cut(s) 660
PstI CTGCAG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
SaqAI TTAA 2 cut(s) 534, 673
SatI GCNGC 3 cut(s) 97, 100, 407
Sau3AI GATC 3 cut(s) 330, 370, 423
Sau96I GGNCC 1 cut(s) 660
SfaNI GCATC 2 cut(s) 430, 518
SfcI CTRYAG 1 cut(s) 178
SinI GGWCC 1 cut(s) 660
Sse9I AATT 7 cut(s) 4, 117, 130, 141, 340, 580, 596
SspI AATATT 1 cut(s) 484
SspMI CTAG 3 cut(s) 278, 299, 368
StyI CCWWGG 2 cut(s) 271, 636
TaaI ACNGT 2 cut(s) 561, 615
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 512
TasI AATT 7 cut(s) 4, 117, 130, 141, 340, 580, 596
TatI WGTACW 1 cut(s) 515
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 2 cut(s) 534, 673
Tru9I TTAA 2 cut(s) 534, 673
TscAI CASTG 2 cut(s) 438, 618
TseI GCWGC 3 cut(s) 96, 99, 406
TspDTI ATGAA 3 cut(s) 17, 141, 210
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 2 cut(s) 438, 618
VpaK11BI GGWCC 1 cut(s) 660
XapI RAATTY 2 cut(s) 4, 130
XbaI TCTAGA 1 cut(s) 367
XceI RCATGY 1 cut(s) 522
XspI CTAG 3 cut(s) 278, 299, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.