RchiOBHm_Chr7g0224561

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000337
Physical Location & Seq
Reverse (-)
46767644 .. 46773868
6225 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20108

Sequence Viewer

Length: 1269 bp
ATGGGGTCTCTCTCTAAACACTGCATAGAGCTGAGGAATCGGAGGGGCTGCGAGTGTATCTGTAATTTGTTAAATGGGTTGAAACCAGTGCTGGGAATGCTGTTGGTAAGTGTTTTAGGTGCAGTGTTGAACATATTCTACAAACTGGTCGCTATTGATGGAATGAACTTGAGGGTCTTCGTCGCCTACCGCGTCATCTTGGCCACTGTCTTCTTGGTGCCTCTCGCTCACGTAGTTGAAAGGAACAAAAGACCAAAACTTACGTGGATTATACTACTGCAAGCATTTCTCTGTGGGTTATTTGGGGTAATACTGGCTCTAAACCTATACCTGGAGAGCATAGTATTGGTATCTGCTACATTCGCTGCCGCGATGAATAATCTGTTACCCGCCATCACCTTCATAATGGCGGTATGTTTGAGGATGGAGAAATTAGTCATAAGAAGCGTTCCAGGGGCGGCGAAAGTTGTCGGCTCACTGGTCGGGATCGGAGGGGCGATGATCTTCACTCTCTACAAAGGCACAATTATACAAATCTGGTCTACACATCCTCACCTTTTTCATATGAACAAAAACACTTCTTCATCACAGGCAAATCACTCTGCCAATCCAGCCTTGGGCTCTGTTTTAGCTGTAGTCAGCTGTTTATGCGCTGCAACTTGGTACATAATTCAGGGTAAAATGAGTAAAACATACCCATGCGTATTGTCCTGCACAGCTCTAGTCTCTGTAATGGCAACAATCCAATCTGTCATTTTGGCCTTATGTATGGAAAGGGACTGGAGTAAATGGAAGTTGGGTTGGAATATTAGACTTCTTGGAGTTGTTTATTCGGGAATAGTGAGTTCAGGACTTGCAATGTGCTTGACTTTATGGTGTATGAATATGAGAGGGCCGTTGTTTGTATCAAGTTTTAACCCTGTAACGCTCATAGTCGTAGCCATTTTCGGCTCTTTGCTGCTCCAAGAGAAGTTGTACCTAGGAAGCATACTTGGAGCATCGCTAATTATATGTGGATTATACGTACTATTGTGGGGTAAAAGCAAGGAAATGAAGAAGCCGATGAGAGTCGAACCGGTACTTGAAACCCCGCGACCGTCCGAAACTCAAGCCATTGAAATTGTTACTTCAACATCTCCTATAGAGCCGAGCGTTAACACCAGTACTGAGAATGATAATAACAACAACACAAATGATAGTTTAATTGCTGTTGGACACTTGGACACCATGAACTTGGAAATAGAGATCGACTTCAGACTACGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

422

Amino Acids

46.15

Weight (kDa)

9.11

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 30 - 163 6.6e-13 EamA-like transporter family
EamA PF00892 206 - 344 7.2e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccI GTMKAC 1 cut(s) 542
AccII CGCG 3 cut(s) 192, 371, 1093
AciI CCGC 6 cut(s) 190, 369, 390, 410, 458, 1091
AclWI GGATC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 1237
AfaI GTAC 5 cut(s) 665, 977, 1026, 1080, 1165
AfiI CCNNNNNNNGG 3 cut(s) 92, 331, 617
AgeI ACCGGT 1 cut(s) 1075
AgsI TTSAA 6 cut(s) 82, 130, 239, 1085, 1118, 1131
AjnI CCWGG 2 cut(s) 330, 451
AluBI AGCT 5 cut(s) 31, 632, 642, 719, 1266
AluI AGCT 5 cut(s) 31, 632, 642, 719, 1266
Alw26I GTCTC 2 cut(s) 12, 730
AlwI GGATC 1 cut(s) 494
AoxI GGCC 3 cut(s) 201, 759, 893
ApeKI GCWGC 4 cut(s) 48, 365, 653, 958
AsiGI ACCGGT 1 cut(s) 1075
Asp700I GAANNNNTTC 1 cut(s) 134
AspA2I CCTAGG 1 cut(s) 979
AspLEI GCGC 1 cut(s) 653
AspS9I GGNCC 1 cut(s) 893
AsuHPI GGTGA 2 cut(s) 388, 545
AvrII CCTAGG 1 cut(s) 979
BalI TGGCCA 1 cut(s) 203
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 623
BbsI GAAGAC 2 cut(s) 169, 202
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 4 cut(s) 35, 352, 640, 945
BccI CCATC 3 cut(s) 152, 401, 418
BceAI ACGGC 1 cut(s) 880
BciT130I CCWGG 2 cut(s) 332, 453
BcoDI GTCTC 2 cut(s) 12, 730
BfaI CTAG 2 cut(s) 722, 980
BfmI CTRYAG 2 cut(s) 633, 1140
BisI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
BlnI CCTAGG 1 cut(s) 979
BlsI GCNGC 6 cut(s) 50, 367, 370, 460, 655, 960
BmcAI AGTACT 1 cut(s) 1165
Bme1390I CCNGG 2 cut(s) 332, 453
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 2 cut(s) 332, 453
BmsI GCATC 1 cut(s) 1007
BpiI GAAGAC 2 cut(s) 169, 202
BpmI CTGGAG 2 cut(s) 353, 802
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 190, 1092
BsaAI YACGTR 3 cut(s) 232, 264, 1024
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 452, 615, 979
BsaWI WCCGGW 1 cut(s) 1075
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 331, 617
Bse118I RCCGGY 1 cut(s) 1075
Bse1I ACTGG 6 cut(s) 86, 150, 318, 483, 785, 1161
Bse3DI GCAATG 1 cut(s) 864
BseBI CCWGG 2 cut(s) 332, 453
BseDI CCNNGG 3 cut(s) 452, 615, 979
BseGI GGATG 2 cut(s) 429, 547
BseLI CCNNNNNNNGG 3 cut(s) 92, 331, 617
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 2 cut(s) 23, 1158
BseNI ACTGG 6 cut(s) 86, 150, 318, 483, 785, 1161
BseXI GCAGC 4 cut(s) 35, 352, 640, 945
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 2 cut(s) 141, 697
Bsh1236I CGCG 3 cut(s) 192, 371, 1093
Bsh1285I CGRYCG 1 cut(s) 1097
BshFI GGCC 3 cut(s) 203, 761, 895
BshNI GGYRCC 1 cut(s) 217
BshTI ACCGGT 1 cut(s) 1075
BsiEI CGRYCG 1 cut(s) 1097
BsiSI CCGG 1 cut(s) 1076
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 3 cut(s) 92, 331, 617
BsmAI GTCTC 2 cut(s) 12, 730
BsmFI GGGAC 1 cut(s) 791
BsmI GAATGC 1 cut(s) 102
BsnI GGCC 3 cut(s) 203, 761, 895
Bso31I GGTCTC 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 623
Bsp143I GATC 3 cut(s) 486, 501, 1245
BspACI CCGC 6 cut(s) 190, 369, 390, 410, 458, 1091
BspANI GGCC 3 cut(s) 203, 761, 895
BspCNI CTCAG 2 cut(s) 24, 1159
BspFNI CGCG 3 cut(s) 192, 371, 1093
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 864
BsrFI RCCGGY 1 cut(s) 1075
BsrI ACTGG 6 cut(s) 86, 150, 318, 483, 785, 1161
BssAI RCCGGY 1 cut(s) 1075
BssECI CCNNGG 3 cut(s) 452, 615, 979
BssMI GATC 3 cut(s) 486, 501, 1245
BssT1I CCWWGG 2 cut(s) 615, 979
Bst2UI CCWGG 2 cut(s) 332, 453
Bst4CI ACNGT 2 cut(s) 208, 1098
BstBAI YACGTR 3 cut(s) 232, 264, 1024
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 2 cut(s) 32, 1167
BstF5I GGATG 2 cut(s) 429, 547
BstFNI CGCG 3 cut(s) 192, 371, 1093
BstHHI GCGC 1 cut(s) 653
BstKTI GATC 3 cut(s) 489, 504, 1248
BstMAI GTCTC 2 cut(s) 12, 730
BstMBI GATC 3 cut(s) 486, 501, 1245
BstMCI CGRYCG 1 cut(s) 1097
BstMWI GCNNNNNNNGC 4 cut(s) 97, 362, 611, 648
BstNI CCWGG 2 cut(s) 332, 453
BstSCI CCNGG 2 cut(s) 330, 451
BstSFI CTRYAG 2 cut(s) 633, 1140
BstSNI TACGTA 1 cut(s) 1024
BstUI CGCG 3 cut(s) 192, 371, 1093
BstV1I GCAGC 4 cut(s) 35, 352, 640, 945
BstV2I GAAGAC 2 cut(s) 169, 202
BstXI CCANNNNNNTGG 1 cut(s) 1234
BsuRI GGCC 3 cut(s) 203, 761, 895
BtgZI GCGATG 3 cut(s) 386, 512, 984
BtsCI GGATG 2 cut(s) 429, 547
BtsI GCAGTG 2 cut(s) 19, 129
BtsIMutI CAGTG 5 cut(s) 19, 93, 129, 204, 476
Cac8I GCNNGC 1 cut(s) 282
CfoI GCGC 1 cut(s) 653
Cfr10I RCCGGY 1 cut(s) 1075
Cfr13I GGNCC 1 cut(s) 893
CseI GACGC 1 cut(s) 181
Csp6I GTAC 5 cut(s) 664, 976, 1025, 1079, 1164
CspAI ACCGGT 1 cut(s) 1075
CviAII CATG 2 cut(s) 699, 1228
CviQI GTAC 5 cut(s) 664, 976, 1025, 1079, 1164
DdeI CTNAG 2 cut(s) 32, 1167
DpnI GATC 3 cut(s) 488, 503, 1247
DpnII GATC 3 cut(s) 486, 501, 1245
EaeI YGGCCR 1 cut(s) 201
Eco105I TACGTA 1 cut(s) 1024
Eco130I CCWWGG 2 cut(s) 615, 979
Eco24I GRGCYC 1 cut(s) 623
Eco31I GGTCTC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 1237
EcoRII CCWGG 2 cut(s) 330, 451
EcoT14I CCWWGG 2 cut(s) 615, 979
EcoT38I GRGCYC 1 cut(s) 623
ErhI CCWWGG 2 cut(s) 615, 979
FaeI CATG 2 cut(s) 702, 1231
FaqI GGGAC 1 cut(s) 791
FatI CATG 2 cut(s) 698, 1227
FauI CCCGC 2 cut(s) 397, 1098
FauNDI CATATG 1 cut(s) 564
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
FokI GGATG 2 cut(s) 436, 534
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
FspBI CTAG 2 cut(s) 722, 980
GlaI GCGC 1 cut(s) 652
GluI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 2 cut(s) 353, 802
HaeIII GGCC 3 cut(s) 203, 761, 895
HapII CCGG 1 cut(s) 1076
HgaI GACGC 1 cut(s) 181
HhaI GCGC 1 cut(s) 653
Hin1II CATG 2 cut(s) 702, 1231
Hin6I GCGC 1 cut(s) 651
HinP1I GCGC 1 cut(s) 651
HincII GTYRAC 1 cut(s) 1156
HindII GTYRAC 1 cut(s) 1156
HinfI GANTC 2 cut(s) 37, 1068
HpaI GTTAAC 1 cut(s) 1156
HpaII CCGG 1 cut(s) 1076
HphI GGTGA 2 cut(s) 388, 545
Hpy166II GTNNAC 2 cut(s) 543, 1156
Hpy188I TCNGA 4 cut(s) 42, 491, 1102, 1256
Hpy188III TCNNGA 3 cut(s) 484, 834, 849
Hpy8I GTNNAC 2 cut(s) 543, 1156
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 409
HpyCH4III ACNGT 2 cut(s) 208, 1098
HpyCH4IV ACGT 3 cut(s) 231, 263, 1023
HpyCH4V TGCA 6 cut(s) 24, 122, 280, 656, 714, 857
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 362, 611, 648
HpyF3I CTNAG 2 cut(s) 32, 1167
HpySE526I ACGT 3 cut(s) 231, 263, 1023
Hsp92II CATG 2 cut(s) 702, 1231
HspAI GCGC 1 cut(s) 651
KspAI GTTAAC 1 cut(s) 1156
Kzo9I GATC 3 cut(s) 486, 501, 1245
LmnI GCTCC 3 cut(s) 966, 995, 1263
Lsp1109I GCAGC 4 cut(s) 35, 352, 640, 945
LweI GCATC 1 cut(s) 1007
MaeI CTAG 2 cut(s) 722, 980
MaeII ACGT 3 cut(s) 231, 263, 1023
MaeIII GTNAC 3 cut(s) 384, 922, 1123
MalI GATC 3 cut(s) 488, 503, 1247
MboI GATC 3 cut(s) 486, 501, 1245
MboII GAAGA 5 cut(s) 169, 202, 496, 573, 1066
MhlI GDGCHC 1 cut(s) 623
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 7 cut(s) 64, 431, 525, 669, 1005, 1119, 1203
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 1 cut(s) 1077
MmeI TCCRAC 2 cut(s) 782, 1192
MnlI CCTC 8 cut(s) 27, 36, 165, 231, 414, 485, 561, 884
Mox20I TGGCCA 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 134
MscI TGGCCA 1 cut(s) 203
MseI TTAA 4 cut(s) 71, 915, 1155, 1202
MslI CAYNNNNRTG 1 cut(s) 697
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 642
MspI CCGG 1 cut(s) 1076
MspR9I CCNGG 2 cut(s) 332, 453
Mva1269I GAATGC 1 cut(s) 102
MvaI CCWGG 2 cut(s) 332, 453
MvnI CGCG 3 cut(s) 192, 371, 1093
MwoI GCNNNNNNNGC 4 cut(s) 97, 362, 611, 648
NdeI CATATG 1 cut(s) 564
NdeII GATC 3 cut(s) 486, 501, 1245
NlaIII CATG 2 cut(s) 702, 1231
NlaIV GGNNCC 1 cut(s) 219
NmeAIII GCCGAG 1 cut(s) 1173
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 37
PinAI ACCGGT 1 cut(s) 1075
PkrI GCNGC 6 cut(s) 50, 367, 370, 460, 655, 960
PleI GAGTC 1 cut(s) 1076
PpsI GAGTC 1 cut(s) 1076
Ppu21I YACGTR 3 cut(s) 232, 264, 1024
Psp6I CCWGG 2 cut(s) 330, 451
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 2 cut(s) 330, 451
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 893
PsrI GAACNNNNNNTAC 2 cut(s) 122, 154
PvuII CAGCTG 1 cut(s) 642
RsaI GTAC 5 cut(s) 665, 977, 1026, 1080, 1165
RsaNI GTAC 5 cut(s) 664, 976, 1025, 1079, 1164
RseI CAYNNNNRTG 1 cut(s) 697
SaqAI TTAA 4 cut(s) 71, 915, 1155, 1202
SatI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
Sau3AI GATC 3 cut(s) 486, 501, 1245
Sau96I GGNCC 1 cut(s) 893
ScaI AGTACT 1 cut(s) 1165
SchI GAGTC 1 cut(s) 1077
ScrFI CCNGG 2 cut(s) 332, 453
SduI GDGCHC 1 cut(s) 623
SfaNI GCATC 1 cut(s) 1007
SfcI CTRYAG 2 cut(s) 633, 1140
SmiMI CAYNNNNRTG 1 cut(s) 697
SmlI CTYRAG 2 cut(s) 169, 1107
SmoI CTYRAG 2 cut(s) 169, 1107
SnaBI TACGTA 1 cut(s) 1024
Sse9I AATT 7 cut(s) 64, 431, 525, 669, 1005, 1119, 1203
SsiI CCGC 6 cut(s) 190, 369, 390, 410, 458, 1091
SspI AATATT 1 cut(s) 808
SspMI CTAG 2 cut(s) 722, 980
StyD4I CCNGG 2 cut(s) 330, 451
StyI CCWWGG 2 cut(s) 615, 979
TaaI ACNGT 2 cut(s) 208, 1098
TaiI ACGT 3 cut(s) 234, 266, 1026
TaqI TCGA 2 cut(s) 1071, 1248
TasI AATT 7 cut(s) 64, 431, 525, 669, 1005, 1119, 1203
TatI WGTACW 1 cut(s) 1163
TauI GCSGC 2 cut(s) 371, 461
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 4 cut(s) 71, 915, 1155, 1202
Tru9I TTAA 4 cut(s) 71, 915, 1155, 1202
TscAI CASTG 5 cut(s) 26, 93, 129, 211, 483
TseI GCWGC 4 cut(s) 48, 365, 653, 958
TspDTI ATGAA 9 cut(s) 179, 389, 391, 551, 573, 581, 896, 1067, 1244
TspRI CASTG 5 cut(s) 26, 93, 129, 211, 483
XcmI CCANNNNNNNNNTGG 3 cut(s) 211, 261, 613
XmaJI CCTAGG 1 cut(s) 979
XmiI GTMKAC 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 2 cut(s) 722, 980
ZrmI AGTACT 1 cut(s) 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.