WAT1-related protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 914 |
| Acc65I | GGTACC | 1 cut(s) | 1035 |
| AccB1I | GGYRCC | 1 cut(s) | 1035 |
| AccIII | TCCGGA | 1 cut(s) | 376 |
| AclWI | GGATC | 2 cut(s) | 10, 231 |
| AcoI | YGGCCR | 2 cut(s) | 114, 390 |
| AcsI | RAATTY | 3 cut(s) | 1018, 1055, 1100 |
| AfaI | GTAC | 2 cut(s) | 543, 1037 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 177, 307, 942 |
| AflII | CTTAAG | 1 cut(s) | 509 |
| AgsI | TTSAA | 1 cut(s) | 1295 |
| AjnI | CCWGG | 3 cut(s) | 116, 826, 1083 |
| AluBI | AGCT | 4 cut(s) | 63, 280, 508, 820 |
| AluI | AGCT | 4 cut(s) | 63, 280, 508, 820 |
| AlwI | GGATC | 2 cut(s) | 10, 231 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 58, 1226 |
| Aor13HI | TCCGGA | 1 cut(s) | 376 |
| AoxI | GGCC | 5 cut(s) | 97, 114, 390, 921, 925 |
| ApeKI | GCWGC | 3 cut(s) | 63, 280, 987 |
| ApoI | RAATTY | 3 cut(s) | 1018, 1055, 1100 |
| Asp718I | GGTACC | 1 cut(s) | 1035 |
| AspS9I | GGNCC | 4 cut(s) | 98, 200, 921, 1118 |
| AsuC2I | CCSGG | 1 cut(s) | 101 |
| AsuHPI | GGTGA | 2 cut(s) | 730, 890 |
| AvaII | GGWCC | 2 cut(s) | 200, 1118 |
| BalI | TGGCCA | 2 cut(s) | 116, 392 |
| BanI | GGYRCC | 1 cut(s) | 1035 |
| BanII | GRGCYC | 1 cut(s) | 314 |
| BbvI | GCAGC | 3 cut(s) | 50, 267, 974 |
| BccI | CCATC | 4 cut(s) | 175, 445, 590, 1083 |
| BciT130I | CCWGG | 3 cut(s) | 118, 828, 1085 |
| BcnI | CCSGG | 1 cut(s) | 101 |
| BfmI | CTRYAG | 3 cut(s) | 672, 1107, 1218 |
| BfrI | CTTAAG | 1 cut(s) | 509 |
| BisI | GCNGC | 3 cut(s) | 64, 281, 988 |
| BlpI | GCTNAGC | 1 cut(s) | 59 |
| BlsI | GCNGC | 3 cut(s) | 65, 282, 989 |
| Bme1390I | CCNGG | 4 cut(s) | 101, 118, 828, 1085 |
| Bme18I | GGWCC | 2 cut(s) | 200, 1118 |
| BmgT120I | GGNCC | 4 cut(s) | 98, 200, 921, 1118 |
| BmiI | GGNNCC | 3 cut(s) | 187, 202, 1037 |
| BmrFI | CCNGG | 4 cut(s) | 101, 118, 828, 1085 |
| BmrI | ACTGGG | 1 cut(s) | 265 |
| BmsI | GCATC | 4 cut(s) | 894, 949, 1061, 1256 |
| BmuI | ACTGGG | 1 cut(s) | 265 |
| BpmI | CTGGAG | 2 cut(s) | 849, 1067 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 59 |
| BpuEI | CTTGAG | 1 cut(s) | 521 |
| BpuMI | CCSGG | 1 cut(s) | 101 |
| Bsa29I | ATCGAT | 1 cut(s) | 1069 |
| BsaJI | CCNNGG | 5 cut(s) | 35, 393, 844, 1084, 1114 |
| BsaWI | WCCGGW | 1 cut(s) | 376 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1080, 1110 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 177, 307, 942 |
| Bse118I | RCCGGY | 1 cut(s) | 923 |
| Bse1I | ACTGG | 2 cut(s) | 260, 750 |
| BseAI | TCCGGA | 1 cut(s) | 376 |
| BseBI | CCWGG | 3 cut(s) | 118, 828, 1085 |
| BseCI | ATCGAT | 1 cut(s) | 1069 |
| BseDI | CCNNGG | 5 cut(s) | 35, 393, 844, 1084, 1114 |
| BseGI | GGATG | 3 cut(s) | 186, 373, 952 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 177, 307, 942 |
| BseMII | CTCAG | 7 cut(s) | 50, 120, 241, 438, 525, 1218, 1242 |
| BseNI | ACTGG | 2 cut(s) | 260, 750 |
| BseRI | GAGGAG | 2 cut(s) | 58, 518 |
| BseXI | GCAGC | 3 cut(s) | 50, 267, 974 |
| BshFI | GGCC | 5 cut(s) | 99, 116, 392, 923, 927 |
| BshNI | GGYRCC | 1 cut(s) | 1035 |
| BshVI | ATCGAT | 1 cut(s) | 1069 |
| BsiSI | CCGG | 5 cut(s) | 101, 155, 377, 924, 1272 |
| BslFI | GGGAC | 3 cut(s) | 229, 515, 1283 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 177, 307, 942 |
| BsmFI | GGGAC | 3 cut(s) | 229, 515, 1283 |
| BsnI | GGCC | 5 cut(s) | 99, 116, 392, 923, 927 |
| Bsp1286I | GDGCHC | 1 cut(s) | 314 |
| Bsp13I | TCCGGA | 1 cut(s) | 376 |
| Bsp1407I | TGTACA | 1 cut(s) | 541 |
| Bsp143I | GATC | 3 cut(s) | 15, 223, 1066 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 59 |
| BspANI | GGCC | 5 cut(s) | 99, 116, 392, 923, 927 |
| BspCNI | CTCAG | 7 cut(s) | 51, 119, 240, 439, 526, 1219, 1243 |
| BspDI | ATCGAT | 1 cut(s) | 1069 |
| BspEI | TCCGGA | 1 cut(s) | 376 |
| BspLI | GGNNCC | 3 cut(s) | 187, 202, 1037 |
| BspMAI | CTGCAG | 2 cut(s) | 1111, 1222 |
| BspPI | GGATC | 2 cut(s) | 10, 231 |
| BspT107I | GGYRCC | 1 cut(s) | 1035 |
| BspTI | CTTAAG | 1 cut(s) | 509 |
| BsrFI | RCCGGY | 1 cut(s) | 923 |
| BsrGI | TGTACA | 1 cut(s) | 541 |
| BsrI | ACTGG | 2 cut(s) | 260, 750 |
| BssAI | RCCGGY | 1 cut(s) | 923 |
| BssECI | CCNNGG | 5 cut(s) | 35, 393, 844, 1084, 1114 |
| BssMI | GATC | 3 cut(s) | 15, 223, 1066 |
| BssT1I | CCWWGG | 2 cut(s) | 393, 1114 |
| Bst2UI | CCWGG | 3 cut(s) | 118, 828, 1085 |
| BstAFI | CTTAAG | 1 cut(s) | 509 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 891 |
| BstAUI | TGTACA | 1 cut(s) | 541 |
| BstC8I | GCNNGC | 2 cut(s) | 925, 1218 |
| BstDEI | CTNAG | 8 cut(s) | 59, 106, 227, 447, 534, 821, 1227, 1251 |
| BstF5I | GGATG | 3 cut(s) | 186, 373, 952 |
| BstKTI | GATC | 3 cut(s) | 18, 226, 1069 |
| BstMBI | GATC | 3 cut(s) | 15, 223, 1066 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 160, 891 |
| BstNI | CCWGG | 3 cut(s) | 118, 828, 1085 |
| BstNSI | RCATGY | 2 cut(s) | 238, 1288 |
| BstSCI | CCNGG | 4 cut(s) | 99, 116, 826, 1083 |
| BstSFI | CTRYAG | 3 cut(s) | 672, 1107, 1218 |
| BstV1I | GCAGC | 3 cut(s) | 50, 267, 974 |
| BstX2I | RGATCY | 1 cut(s) | 223 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 197 |
| BstYI | RGATCY | 1 cut(s) | 223 |
| Bsu15I | ATCGAT | 1 cut(s) | 1069 |
| BsuRI | GGCC | 5 cut(s) | 99, 116, 392, 923, 927 |
| BsuTUI | ATCGAT | 1 cut(s) | 1069 |
| BtsCI | GGATG | 3 cut(s) | 186, 373, 952 |
| BtsIMutI | CAGTG | 1 cut(s) | 235 |
| Cac8I | GCNNGC | 2 cut(s) | 925, 1218 |
| CaiI | CAGNNNCTG | 2 cut(s) | 58, 1226 |
| Cfr10I | RCCGGY | 1 cut(s) | 923 |
| Cfr13I | GGNCC | 4 cut(s) | 98, 200, 921, 1118 |
| ClaI | ATCGAT | 1 cut(s) | 1069 |
| Csp6I | GTAC | 2 cut(s) | 542, 1036 |
| CviAII | CATG | 7 cut(s) | 191, 235, 646, 706, 863, 895, 1285 |
| CviQI | GTAC | 2 cut(s) | 542, 1036 |
| DdeI | CTNAG | 8 cut(s) | 59, 106, 227, 447, 534, 821, 1227, 1251 |
| DpnI | GATC | 3 cut(s) | 17, 225, 1068 |
| DpnII | GATC | 3 cut(s) | 15, 223, 1066 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 914 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 914 |
| EaeI | YGGCCR | 2 cut(s) | 114, 390 |
| Eco130I | CCWWGG | 2 cut(s) | 393, 1114 |
| Eco24I | GRGCYC | 1 cut(s) | 314 |
| Eco47I | GGWCC | 2 cut(s) | 200, 1118 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1118 |
| EcoRII | CCWGG | 3 cut(s) | 116, 826, 1083 |
| EcoT14I | CCWWGG | 2 cut(s) | 393, 1114 |
| EcoT38I | GRGCYC | 1 cut(s) | 314 |
| ErhI | CCWWGG | 2 cut(s) | 393, 1114 |
| FaeI | CATG | 7 cut(s) | 194, 238, 649, 709, 866, 898, 1288 |
| FaqI | GGGAC | 3 cut(s) | 229, 515, 1283 |
| FatI | CATG | 7 cut(s) | 190, 234, 645, 705, 862, 894, 1284 |
| Fnu4HI | GCNGC | 3 cut(s) | 64, 281, 988 |
| FokI | GGATG | 3 cut(s) | 193, 360, 959 |
| FriOI | GRGCYC | 1 cut(s) | 314 |
| FseI | GGCCGGCC | 1 cut(s) | 927 |
| Fsp4HI | GCNGC | 3 cut(s) | 64, 281, 988 |
| GluI | GCNGC | 3 cut(s) | 64, 281, 988 |
| GsuI | CTGGAG | 2 cut(s) | 849, 1067 |
| HaeIII | GGCC | 5 cut(s) | 99, 116, 392, 923, 927 |
| HapII | CCGG | 5 cut(s) | 101, 155, 377, 924, 1272 |
| Hin1II | CATG | 7 cut(s) | 194, 238, 649, 709, 866, 898, 1288 |
| HincII | GTYRAC | 1 cut(s) | 729 |
| HindII | GTYRAC | 1 cut(s) | 729 |
| HindIII | AAGCTT | 1 cut(s) | 818 |
| HinfI | GANTC | 5 cut(s) | 104, 194, 214, 1091, 1152 |
| HpaII | CCGG | 5 cut(s) | 101, 155, 377, 924, 1272 |
| HphI | GGTGA | 2 cut(s) | 730, 890 |
| Hpy166II | GTNNAC | 3 cut(s) | 701, 729, 1279 |
| Hpy188I | TCNGA | 8 cut(s) | 54, 75, 109, 145, 316, 848, 907, 1252 |
| Hpy188III | TCNNGA | 9 cut(s) | 198, 211, 221, 377, 500, 752, 773, 944, 1000 |
| Hpy8I | GTNNAC | 3 cut(s) | 701, 729, 1279 |
| Hpy99I | CGWCG | 1 cut(s) | 1136 |
| HpyAV | CCTTC | 1 cut(s) | 1131 |
| HpyCH4V | TGCA | 8 cut(s) | 403, 593, 885, 894, 1109, 1220, 1247, 1258 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 160, 891 |
| HpyF3I | CTNAG | 8 cut(s) | 59, 106, 227, 447, 534, 821, 1227, 1251 |
| Hsp92II | CATG | 7 cut(s) | 194, 238, 649, 709, 866, 898, 1288 |
| Kpn2I | TCCGGA | 1 cut(s) | 376 |
| KpnI | GGTACC | 1 cut(s) | 1039 |
| KroI | GCCGGC | 1 cut(s) | 923 |
| KroNI | GCCGGC | 1 cut(s) | 925 |
| Kzo9I | GATC | 3 cut(s) | 15, 223, 1066 |
| LmnI | GCTCC | 3 cut(s) | 505, 739, 848 |
| Lsp1109I | GCAGC | 3 cut(s) | 50, 267, 974 |
| LweI | GCATC | 4 cut(s) | 894, 949, 1061, 1256 |
| MaeIII | GTNAC | 4 cut(s) | 667, 878, 1027, 1135 |
| MalI | GATC | 3 cut(s) | 17, 225, 1068 |
| MboI | GATC | 3 cut(s) | 15, 223, 1066 |
| MboII | GAAGA | 3 cut(s) | 103, 614, 1070 |
| MflI | RGATCY | 1 cut(s) | 223 |
| MhlI | GDGCHC | 1 cut(s) | 314 |
| MlsI | TGGCCA | 2 cut(s) | 116, 392 |
| MluCI | AATT | 7 cut(s) | 133, 147, 755, 1018, 1055, 1100, 1168 |
| MluNI | TGGCCA | 2 cut(s) | 116, 392 |
| MlyI | GAGTC | 1 cut(s) | 98 |
| Mox20I | TGGCCA | 2 cut(s) | 116, 392 |
| MroI | TCCGGA | 1 cut(s) | 376 |
| MroNI | GCCGGC | 1 cut(s) | 923 |
| MscI | TGGCCA | 2 cut(s) | 116, 392 |
| MseI | TTAA | 3 cut(s) | 333, 510, 1016 |
| MslI | CAYNNNNRTG | 2 cut(s) | 239, 945 |
| Msp20I | TGGCCA | 2 cut(s) | 116, 392 |
| MspCI | CTTAAG | 1 cut(s) | 509 |
| MspI | CCGG | 5 cut(s) | 101, 155, 377, 924, 1272 |
| MspR9I | CCNGG | 4 cut(s) | 101, 118, 828, 1085 |
| MvaI | CCWGG | 3 cut(s) | 118, 828, 1085 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 160, 891 |
| NaeI | GCCGGC | 1 cut(s) | 925 |
| NciI | CCSGG | 1 cut(s) | 101 |
| NdeII | GATC | 3 cut(s) | 15, 223, 1066 |
| NgoMIV | GCCGGC | 1 cut(s) | 923 |
| NlaIII | CATG | 7 cut(s) | 194, 238, 649, 709, 866, 898, 1288 |
| NlaIV | GGNNCC | 3 cut(s) | 187, 202, 1037 |
| NmeAIII | GCCGAG | 2 cut(s) | 60, 196 |
| NmuCI | GTSAC | 3 cut(s) | 878, 1027, 1135 |
| NspI | RCATGY | 2 cut(s) | 238, 1288 |
| PdiI | GCCGGC | 1 cut(s) | 925 |
| PfeI | GAWTC | 4 cut(s) | 194, 214, 1091, 1152 |
| PfoI | TCCNGGA | 1 cut(s) | 826 |
| PkrI | GCNGC | 3 cut(s) | 65, 282, 989 |
| PleI | GAGTC | 1 cut(s) | 98 |
| PpsI | GAGTC | 1 cut(s) | 98 |
| PpuMI | RGGWCCY | 1 cut(s) | 1118 |
| Psp5II | RGGWCCY | 1 cut(s) | 1118 |
| Psp6I | CCWGG | 3 cut(s) | 116, 826, 1083 |
| PspGI | CCWGG | 3 cut(s) | 116, 826, 1083 |
| PspN4I | GGNNCC | 3 cut(s) | 187, 202, 1037 |
| PspPI | GGNCC | 4 cut(s) | 98, 200, 921, 1118 |
| PspPPI | RGGWCCY | 1 cut(s) | 1118 |
| PstI | CTGCAG | 2 cut(s) | 1111, 1222 |
| PstNI | CAGNNNCTG | 2 cut(s) | 58, 1226 |
| PsuI | RGATCY | 1 cut(s) | 223 |
| RigI | GGCCGGCC | 1 cut(s) | 927 |
| RsaI | GTAC | 2 cut(s) | 543, 1037 |
| RsaNI | GTAC | 2 cut(s) | 542, 1036 |
| RseI | CAYNNNNRTG | 2 cut(s) | 239, 945 |
| SaqAI | TTAA | 3 cut(s) | 333, 510, 1016 |
| SatI | GCNGC | 3 cut(s) | 64, 281, 988 |
| Sau3AI | GATC | 3 cut(s) | 15, 223, 1066 |
| Sau96I | GGNCC | 4 cut(s) | 98, 200, 921, 1118 |
| SchI | GAGTC | 1 cut(s) | 98 |
| ScrFI | CCNGG | 4 cut(s) | 101, 118, 828, 1085 |
| SduI | GDGCHC | 1 cut(s) | 314 |
| SfaNI | GCATC | 4 cut(s) | 894, 949, 1061, 1256 |
| SfcI | CTRYAG | 3 cut(s) | 672, 1107, 1218 |
| SinI | GGWCC | 2 cut(s) | 200, 1118 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 239, 945 |
| SmlI | CTYRAG | 2 cut(s) | 500, 509 |
| SmoI | CTYRAG | 2 cut(s) | 500, 509 |
| Sse9I | AATT | 7 cut(s) | 133, 147, 755, 1018, 1055, 1100, 1168 |
| StyD4I | CCNGG | 4 cut(s) | 99, 116, 826, 1083 |
| StyI | CCWWGG | 2 cut(s) | 393, 1114 |
| TaqI | TCGA | 3 cut(s) | 362, 888, 1069 |
| TasI | AATT | 7 cut(s) | 133, 147, 755, 1018, 1055, 1100, 1168 |
| TatI | WGTACW | 1 cut(s) | 541 |
| TfiI | GAWTC | 4 cut(s) | 194, 214, 1091, 1152 |
| Tru1I | TTAA | 3 cut(s) | 333, 510, 1016 |
| Tru9I | TTAA | 3 cut(s) | 333, 510, 1016 |
| TscAI | CASTG | 1 cut(s) | 235 |
| TseFI | GTSAC | 3 cut(s) | 878, 1027, 1135 |
| TseI | GCWGC | 3 cut(s) | 63, 280, 987 |
| Tsp45I | GTSAC | 3 cut(s) | 878, 1027, 1135 |
| TspDTI | ATGAA | 3 cut(s) | 44, 206, 870 |
| TspRI | CASTG | 1 cut(s) | 235 |
| Vha464I | CTTAAG | 1 cut(s) | 509 |
| VpaK11BI | GGWCC | 2 cut(s) | 200, 1118 |
| XapI | RAATTY | 3 cut(s) | 1018, 1055, 1100 |
| XceI | RCATGY | 2 cut(s) | 238, 1288 |