Rh7AG378400

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000337
Physical Location & Seq
Forward (+)
49292530 .. 49329264
36735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG378400.1

Sequence Viewer

Length: 1065 bp
ATGGGGAAGCAGAGTAGTGAGAGTAGTGTAGTTGATGGCTTAATGAAGCCAGTGATAACGATGGTGATCGCTCAGATTGCATATGCAGCAACGAGTATATTCTACAAGGTTGCAGCAGACTCTGGAATGAATCTCAGGATTCTCGTTGCCTACCGTCTCATGTTTTCATCTGCCATTATCGTTCCTCTTGCTCTTATACTCGAACGGAACAGCAGGCCCAAACTCACTCTAGTCGTACTCTTTCAAGCTTTTCTCTGCGGATTATTTGGGGGATCATTGTCACAAAATTTATTCATAGAGGGACTGGCCTTAACATCACCGACATTTGTTGCAGCCACTGCAAATCTGATTCCGGCGGTTACCTTGATCATGGCCATCTGTTTTAGGTTGGAGAAGTTGACACTAGGAAGCCATGCAGGGAAGGCAAAGCTTGTGGGGACAGTGGTAGGTATTGGTGGTGCTATGATCTTTACCTTCTTCAAAGGACCAGAGCTCTCCATCTGGTCAACTCACGTTGACCTTCTTCGAGGACACCACGCGGCACCATCACCGTCATCGGTCTCCTTACACAGAAGCACCGGTAGTCACTTGCTGGGCTCCTTCTCGGCTCTCGGCAGCGCCGTTTCCTATGCAATGTGGCTCACAGTTCAGGCAAAAATGAGCAAGAGATATCCATGCCCTTACTCGAGCACAGCTCTAATGTCAGCGATGGGATCGATTCAGTCGGTAGCTTTTGCACTCTGCGTGGAGAGGGATTGGAGCCAGTGGAAATTGGGTTGGAACATCAAGCTTCTCACAGCTGCATATACGGGAATTGTGTCTTCGGGGCTGGTGGTGGTTCTTATCTCGTGGTGTATACGCAAGAGAGGTCCATTGTTCGTGTCAGCTTTCACTCCGCTTTGCCTAGTGATTGTGGCCATTGCTAGTTCTTTGTTGATCAATGAAAAGTTAAGCCTAGGAAGCATATTAGGAGGGATTCTAATTGTATGTGGGTTATATATGGTGCTATGGGGAAAGAGCAAGGAGATGAAGTCGATTAATAATTTAGACAGTGATCAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

354

Amino Acids

37.89

Weight (kDa)

9.55

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 17 - 157 6.6e-13 EamA-like transporter family
EamA PF00892 198 - 336 1.1e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 541
AccI GTMKAC 1 cut(s) 856
AccII CGCG 1 cut(s) 539
AciI CCGC 4 cut(s) 258, 356, 539, 896
AclWI GGATC 2 cut(s) 280, 721
AcoI YGGCCR 2 cut(s) 372, 915
AcsI RAATTY 1 cut(s) 286
AfaI GTAC 1 cut(s) 237
AgeI ACCGGT 1 cut(s) 578
AgsI TTSAA 2 cut(s) 245, 481
AluBI AGCT 8 cut(s) 248, 430, 493, 695, 731, 790, 800, 887
AluI AGCT 8 cut(s) 248, 430, 493, 695, 731, 790, 800, 887
Alw21I GWGCWC 2 cut(s) 495, 692
Alw26I GTCTC 2 cut(s) 161, 565
AlwI GGATC 2 cut(s) 280, 721
AlwNI CAGNNNCTG 2 cut(s) 122, 338
Ama87I CYCGRG 1 cut(s) 685
AoxI GGCC 4 cut(s) 215, 306, 372, 915
ApeKI GCWGC 5 cut(s) 86, 113, 332, 615, 800
ApoI RAATTY 1 cut(s) 286
AseI ATTAAT 1 cut(s) 1038
AsiGI ACCGGT 1 cut(s) 578
AspA2I CCTAGG 1 cut(s) 955
AspLEI GCGC 1 cut(s) 620
AspS9I GGNCC 3 cut(s) 216, 485, 869
AsuHPI GGTGA 3 cut(s) 76, 309, 540
AvaI CYCGRG 1 cut(s) 685
AvaII GGWCC 2 cut(s) 485, 869
AvrII CCTAGG 1 cut(s) 955
BalI TGGCCA 2 cut(s) 374, 917
BanI GGYRCC 1 cut(s) 541
BanII GRGCYC 2 cut(s) 495, 599
BauI CACGAG 1 cut(s) 847
BbsI GAAGAC 1 cut(s) 813
Bbv12I GWGCWC 2 cut(s) 495, 692
BbvI GCAGC 5 cut(s) 98, 125, 344, 627, 787
BccI CCATC 6 cut(s) 29, 55, 383, 506, 553, 703
BceAI ACGGC 1 cut(s) 605
BclI TGATCA 3 cut(s) 366, 936, 1054
BcoDI GTCTC 2 cut(s) 161, 565
BfaI CTAG 5 cut(s) 230, 404, 905, 924, 956
BfoI RGCGCY 1 cut(s) 621
BisI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
BlnI CCTAGG 1 cut(s) 955
BlsI GCNGC 6 cut(s) 88, 115, 334, 541, 617, 802
Bme18I GGWCC 2 cut(s) 485, 869
BmeT110I CYCGRG 1 cut(s) 685
BmgT120I GGNCC 3 cut(s) 216, 485, 869
BmiI GGNNCC 3 cut(s) 543, 598, 761
BpiI GAAGAC 1 cut(s) 813
BplI GAGNNNNNCTC 2 cut(s) 679, 711
Bsa29I ATCGAT 1 cut(s) 716
BsaBI GATNNNNATC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 565
BsaJI CCNNGG 1 cut(s) 955
BsaWI WCCGGW 1 cut(s) 578
BsaXI ACNNNNNCTCC 2 cut(s) 1016, 1046
Bse118I RCCGGY 1 cut(s) 578
Bse1I ACTGG 3 cut(s) 50, 309, 763
Bse3DI GCAATG 2 cut(s) 639, 918
Bse8I GATNNNNATC 1 cut(s) 65
BseCI ATCGAT 1 cut(s) 716
BseDI CCNNGG 1 cut(s) 955
BseJI GATNNNNATC 1 cut(s) 65
BseMI GCAATG 2 cut(s) 639, 918
BseMII CTCAG 2 cut(s) 86, 148
BseNI ACTGG 3 cut(s) 50, 309, 763
BseXI GCAGC 5 cut(s) 98, 125, 344, 627, 787
BseYI CCCAGC 1 cut(s) 592
Bsh1236I CGCG 1 cut(s) 539
BshFI GGCC 4 cut(s) 217, 308, 374, 917
BshNI GGYRCC 1 cut(s) 541
BshTI ACCGGT 1 cut(s) 578
BshVI ATCGAT 1 cut(s) 716
BsiHKAI GWGCWC 2 cut(s) 495, 692
BsiHKCI CYCGRG 1 cut(s) 685
BsiSI CCGG 2 cut(s) 353, 579
BslFI GGGAC 2 cut(s) 315, 451
BsmAI GTCTC 2 cut(s) 161, 565
BsmBI CGTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 315, 451
BsnI GGCC 4 cut(s) 217, 308, 374, 917
Bso31I GGTCTC 1 cut(s) 565
BsoBI CYCGRG 1 cut(s) 685
Bsp1286I GDGCHC 3 cut(s) 495, 599, 692
Bsp143I GATC 7 cut(s) 66, 272, 366, 465, 713, 936, 1054
BspACI CCGC 4 cut(s) 258, 356, 539, 896
BspANI GGCC 4 cut(s) 217, 308, 374, 917
BspCNI CTCAG 2 cut(s) 85, 147
BspDI ATCGAT 1 cut(s) 716
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 3 cut(s) 543, 598, 761
BspPI GGATC 2 cut(s) 280, 721
BspT107I GGYRCC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 565
BsrDI GCAATG 2 cut(s) 639, 918
BsrFI RCCGGY 1 cut(s) 578
BsrI ACTGG 3 cut(s) 50, 309, 763
BssAI RCCGGY 1 cut(s) 578
BssECI CCNNGG 1 cut(s) 955
BssMI GATC 7 cut(s) 66, 272, 366, 465, 713, 936, 1054
BssNAI GTATAC 1 cut(s) 857
BssSI CACGAG 1 cut(s) 847
BssT1I CCWWGG 1 cut(s) 955
Bst1107I GTATAC 1 cut(s) 857
Bst2BI CACGAG 1 cut(s) 847
Bst4CI ACNGT 5 cut(s) 155, 442, 552, 646, 1052
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 2 cut(s) 72, 134
BstEII GGTNACC 1 cut(s) 358
BstFNI CGCG 1 cut(s) 539
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 1 cut(s) 620
BstKTI GATC 7 cut(s) 69, 275, 369, 468, 716, 939, 1057
BstMAI GTCTC 2 cut(s) 161, 565
BstMBI GATC 7 cut(s) 66, 272, 366, 465, 713, 936, 1054
BstMWI GCNNNNNNNGC 5 cut(s) 77, 86, 338, 422, 960
BstPI GGTNACC 1 cut(s) 358
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 5 cut(s) 98, 125, 344, 627, 787
BstV2I GAAGAC 1 cut(s) 813
BstZ17I GTATAC 1 cut(s) 857
Bsu15I ATCGAT 1 cut(s) 716
BsuRI GGCC 4 cut(s) 217, 308, 374, 917
BsuTUI ATCGAT 1 cut(s) 716
BtgZI GCGATG 1 cut(s) 722
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 5 cut(s) 57, 336, 447, 770, 1057
Cac8I GCNNGC 1 cut(s) 215
CaiI CAGNNNCTG 2 cut(s) 122, 338
CfoI GCGC 1 cut(s) 620
Cfr10I RCCGGY 1 cut(s) 578
Cfr13I GGNCC 3 cut(s) 216, 485, 869
ClaI ATCGAT 1 cut(s) 716
Csp6I GTAC 1 cut(s) 236
CspAI ACCGGT 1 cut(s) 578
CspCI CAANNNNNGTGG 2 cut(s) 414, 449
CviAII CATG 4 cut(s) 160, 370, 413, 675
CviQI GTAC 1 cut(s) 236
DdeI CTNAG 2 cut(s) 72, 134
DpnI GATC 7 cut(s) 68, 274, 368, 467, 715, 938, 1056
DpnII GATC 7 cut(s) 66, 272, 366, 465, 713, 936, 1054
EaeI YGGCCR 2 cut(s) 372, 915
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 955
Eco24I GRGCYC 2 cut(s) 495, 599
Eco31I GGTCTC 1 cut(s) 565
Eco32I GATATC 1 cut(s) 671
Eco47I GGWCC 2 cut(s) 485, 869
Eco53kI GAGCTC 1 cut(s) 493
Eco88I CYCGRG 1 cut(s) 685
Eco91I GGTNACC 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 493
EcoO65I GGTNACC 1 cut(s) 358
EcoRV GATATC 1 cut(s) 671
EcoT14I CCWWGG 1 cut(s) 955
EcoT38I GRGCYC 2 cut(s) 495, 599
ErhI CCWWGG 1 cut(s) 955
Esp3I CGTCTC 1 cut(s) 161
FaeI CATG 4 cut(s) 163, 373, 416, 678
FaqI GGGAC 2 cut(s) 315, 451
FatI CATG 4 cut(s) 159, 369, 412, 674
FauNDI CATATG 1 cut(s) 82
FbaI TGATCA 3 cut(s) 366, 936, 1054
FblI GTMKAC 1 cut(s) 856
Fnu4HI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
FriOI GRGCYC 2 cut(s) 495, 599
Fsp4HI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
FspBI CTAG 5 cut(s) 230, 404, 905, 924, 956
GlaI GCGC 1 cut(s) 619
GluI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
GsaI CCCAGC 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 4 cut(s) 217, 308, 374, 917
HapII CCGG 2 cut(s) 353, 579
HhaI GCGC 1 cut(s) 620
Hin1II CATG 4 cut(s) 163, 373, 416, 678
Hin6I GCGC 1 cut(s) 618
HinP1I GCGC 1 cut(s) 618
HincII GTYRAC 3 cut(s) 399, 507, 517
HindII GTYRAC 3 cut(s) 399, 507, 517
HindIII AAGCTT 3 cut(s) 246, 428, 788
HinfI GANTC 6 cut(s) 119, 130, 139, 349, 718, 976
HpaII CCGG 2 cut(s) 353, 579
HphI GGTGA 3 cut(s) 76, 309, 540
Hpy166II GTNNAC 4 cut(s) 399, 507, 517, 857
Hpy188I TCNGA 2 cut(s) 75, 348
Hpy188III TCNNGA 3 cut(s) 123, 136, 1058
Hpy8I GTNNAC 4 cut(s) 399, 507, 517, 857
HpyAV CCTTC 4 cut(s) 415, 484, 530, 610
HpyCH4III ACNGT 5 cut(s) 155, 442, 552, 646, 1052
HpyCH4IV ACGT 1 cut(s) 513
HpyCH4V TGCA 9 cut(s) 80, 86, 113, 332, 341, 416, 632, 737, 803
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 86, 338, 422, 960
HpyF3I CTNAG 2 cut(s) 72, 134
HpySE526I ACGT 1 cut(s) 513
Hsp92II CATG 4 cut(s) 163, 373, 416, 678
HspAI GCGC 1 cut(s) 618
Ksp22I TGATCA 3 cut(s) 366, 936, 1054
Kzo9I GATC 7 cut(s) 66, 272, 366, 465, 713, 936, 1054
LmnI GCTCC 2 cut(s) 602, 759
Lsp1109I GCAGC 5 cut(s) 98, 125, 344, 627, 787
MaeI CTAG 5 cut(s) 230, 404, 905, 924, 956
MaeII ACGT 1 cut(s) 513
MaeIII GTNAC 3 cut(s) 279, 358, 584
MalI GATC 7 cut(s) 68, 274, 368, 467, 715, 938, 1056
MboI GATC 7 cut(s) 66, 272, 366, 465, 713, 936, 1054
MboII GAAGA 3 cut(s) 469, 515, 813
MhlI GDGCHC 3 cut(s) 495, 599, 692
MlsI TGGCCA 2 cut(s) 374, 917
MluCI AATT 5 cut(s) 286, 770, 813, 981, 1042
MluNI TGGCCA 2 cut(s) 374, 917
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 369, 758
MnlI CCTC 6 cut(s) 195, 292, 521, 744, 860, 965
Mox20I TGGCCA 2 cut(s) 374, 917
MscI TGGCCA 2 cut(s) 374, 917
MseI TTAA 4 cut(s) 41, 311, 950, 1038
Msp20I TGGCCA 2 cut(s) 374, 917
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 2 cut(s) 353, 579
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 5 cut(s) 77, 86, 338, 422, 960
NdeI CATATG 1 cut(s) 82
NdeII GATC 7 cut(s) 66, 272, 366, 465, 713, 936, 1054
NlaIII CATG 4 cut(s) 163, 373, 416, 678
NlaIV GGNNCC 3 cut(s) 543, 598, 761
NmeAIII GCCGAG 2 cut(s) 584, 591
NmuCI GTSAC 2 cut(s) 279, 584
PaeR7I CTCGAG 1 cut(s) 685
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PfeI GAWTC 5 cut(s) 130, 139, 349, 718, 976
PinAI ACCGGT 1 cut(s) 578
PkrI GCNGC 6 cut(s) 88, 115, 334, 541, 617, 802
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PshBI ATTAAT 1 cut(s) 1038
Psp124BI GAGCTC 1 cut(s) 495
PspEI GGTNACC 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 592
PspN4I GGNNCC 3 cut(s) 543, 598, 761
PspPI GGNCC 3 cut(s) 216, 485, 869
PspXI VCTCGAGB 1 cut(s) 685
PstNI CAGNNNCTG 2 cut(s) 122, 338
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 1 cut(s) 237
RsaNI GTAC 1 cut(s) 236
SacI GAGCTC 1 cut(s) 495
SaqAI TTAA 4 cut(s) 41, 311, 950, 1038
SatI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
Sau3AI GATC 7 cut(s) 66, 272, 366, 465, 713, 936, 1054
Sau96I GGNCC 3 cut(s) 216, 485, 869
SchI GAGTC 1 cut(s) 113
SduI GDGCHC 3 cut(s) 495, 599, 692
Sfr274I CTCGAG 1 cut(s) 685
SinI GGWCC 2 cut(s) 485, 869
SlaI CTCGAG 1 cut(s) 685
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
Sse9I AATT 5 cut(s) 286, 770, 813, 981, 1042
SsiI CCGC 4 cut(s) 258, 356, 539, 896
SspMI CTAG 5 cut(s) 230, 404, 905, 924, 956
SstI GAGCTC 1 cut(s) 495
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 5 cut(s) 155, 442, 552, 646, 1052
TaiI ACGT 1 cut(s) 516
TaqI TCGA 5 cut(s) 201, 526, 686, 716, 1034
TaqII GACCGA 1 cut(s) 547
TasI AATT 5 cut(s) 286, 770, 813, 981, 1042
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 5 cut(s) 130, 139, 349, 718, 976
Tru1I TTAA 4 cut(s) 41, 311, 950, 1038
Tru9I TTAA 4 cut(s) 41, 311, 950, 1038
TscAI CASTG 5 cut(s) 57, 343, 447, 770, 1057
TseFI GTSAC 2 cut(s) 279, 584
TseI GCWGC 5 cut(s) 86, 113, 332, 615, 800
Tsp45I GTSAC 2 cut(s) 279, 584
TspDTI ATGAA 6 cut(s) 59, 143, 156, 283, 957, 1043
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 5 cut(s) 57, 343, 447, 770, 1057
VpaK11BI GGWCC 2 cut(s) 485, 869
VspI ATTAAT 1 cut(s) 1038
XapI RAATTY 1 cut(s) 286
XhoI CTCGAG 1 cut(s) 685
XmaJI CCTAGG 1 cut(s) 955
XmiI GTMKAC 1 cut(s) 856
XspI CTAG 5 cut(s) 230, 404, 905, 924, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.