Rroxscaffold_3G00234570

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000337
Physical Location & Seq
Forward (+)
21064376 .. 21068744
4369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00234570.1

Sequence Viewer

Length: 1110 bp
ATGGCCGCCGCTCCTCTACCCTACATCACCGCACAAGCATTTGCTTGCCTTCGCCCGACTGCAGATTTCGATGGTTTGATCTACAAATCTCAAGGTCTTGACAATGGTTATATTGTTGGTAATTACCTTGAGGAGGCTGAAGATTTACCTTCAGTTTTTGCAAGTTTTTCTACAAGTGTTTCAGAGCAAGGTTTTATTGTACATGGACAACTTGCTAGTGTAGTGGAAGGGTATCTCTCTCTCTGCATTTTGAAGCCAGTAATACAAATGGGGCAGCAGGGTGGTATTGAGATTTCTAGCACTCCTAGTAGTGTAGTGGATGGATTAATGAAGCCAGTGATAACGATGGTGGCAGCTCAGATTGCATATGCAGCAACGGGTATTTTCTACAAACTTGCAGCAGACTCTGGCATGAACCTCAATATTCTCGTTGCCTACCGTCTCATGTTTTCCTCTGTCATTATCGTTCCTCTTGCTCTTATACTCGAAAGGTTGGAGAAGTTGACACTAGGAAGCCATGCAGGGAAGGCAAAGCTTGTGGGGACAGTGGTAGGTATTGGTGGTGCTATGATCTTTACCTTCTTCAAAGGACCAGAGCTCTCCATCTGGTCAACTCACGTTGACCTTCTTCGAGGACACCACCCGGCACCATCACCTTCATCGGTCTCCTTGCACAGAAGCACCGGTAGTCACTTGCTGGGCTCCTTCTCGGCTCTCGGCAGCGCCGTTTCCTATGCAGTGTGGCTCACAGTTCAGGCAAAAATGAGCAAGAGATATCCATGCCCTTACTCGAGCACAGCTCTAATGTCAGTGATGGGGTCGATTCAGTCGGTAGCTTTTGCACTCTGCGTGGAGAGGGATTGGAGCCAGTGGAGATTGGGTTGGAACATCAAGCTTCTCACAGCCGCATATACGGTAAGATCATCAATCATTAAAAAGAATAACTACATGGGTTTTATATTCGATCTACCTCTTAGGGCTCCCGCTCTGAGCCGTAAAATAAGCAAGTCTATCTACATTGTCCACCATGCGTCAAATAACAAATCTTCAGATACACCGCATTATAGATACTGTCGCACAGTGTATCCGATTGATGGATATAAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

369

Amino Acids

40.02

Weight (kDa)

9.13

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 646
AccBSI CCGCTC 2 cut(s) 11, 986
AciI CCGC 6 cut(s) 6, 9, 30, 906, 984, 1058
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 3 cut(s) 135, 159, 1032
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 2 cut(s) 133, 1094
AgeI ACCGGT 1 cut(s) 683
AgsI TTSAA 2 cut(s) 253, 586
AluBI AGCT 6 cut(s) 356, 535, 598, 800, 836, 895
AluI AGCT 6 cut(s) 356, 535, 598, 800, 836, 895
Alw21I GWGCWC 2 cut(s) 600, 797
Alw26I GTCTC 2 cut(s) 446, 670
AlwNI CAGNNNCTG 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 790
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 5 cut(s) 274, 353, 371, 398, 720
AseI ATTAAT 1 cut(s) 326
AsiGI ACCGGT 1 cut(s) 683
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 1 cut(s) 590
AsuC2I CCSGG 1 cut(s) 644
AsuHPI GGTGA 2 cut(s) 19, 645
AvaI CYCGRG 1 cut(s) 790
AvaII GGWCC 1 cut(s) 590
BanI GGYRCC 1 cut(s) 646
BanII GRGCYC 3 cut(s) 600, 704, 982
Bbv12I GWGCWC 2 cut(s) 600, 797
BbvI GCAGC 5 cut(s) 286, 365, 383, 410, 732
BccI CCATC 7 cut(s) 65, 314, 340, 611, 658, 808, 1088
BceAI ACGGC 2 cut(s) 710, 978
BciVI GTATCC 1 cut(s) 1095
BcnI CCSGG 1 cut(s) 644
BcoDI GTCTC 2 cut(s) 446, 670
BfaI CTAG 4 cut(s) 216, 297, 306, 509
BfmI CTRYAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 726
BfuI GTATCC 1 cut(s) 1095
BisI GCNGC 8 cut(s) 6, 9, 275, 354, 372, 399, 721, 906
BlsI GCNGC 8 cut(s) 7, 10, 276, 355, 373, 400, 722, 907
Bme1390I CCNGG 1 cut(s) 644
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 1 cut(s) 790
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 4 cut(s) 648, 703, 866, 981
BmrFI CCNGG 1 cut(s) 644
BplI GAGNNNNNCTC 2 cut(s) 784, 816
BpuEI CTTGAG 2 cut(s) 75, 149
BpuMI CCSGG 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 670
BsaWI WCCGGW 1 cut(s) 683
BsaXI ACNNNNNCTCC 2 cut(s) 865, 895
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 1094
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 3 cut(s) 257, 335, 868
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 2 cut(s) 133, 1094
BseMII CTCAG 2 cut(s) 371, 980
BseNI ACTGG 3 cut(s) 257, 335, 868
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 5 cut(s) 286, 365, 383, 410, 732
BseYI CCCAGC 1 cut(s) 697
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 646
BshTI ACCGGT 1 cut(s) 683
BsiHKAI GWGCWC 2 cut(s) 600, 797
BsiHKCI CYCGRG 1 cut(s) 790
BsiSI CCGG 2 cut(s) 644, 684
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 2 cut(s) 133, 1094
BsmAI GTCTC 2 cut(s) 446, 670
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 670
BsoBI CYCGRG 1 cut(s) 790
Bsp1286I GDGCHC 4 cut(s) 600, 704, 797, 982
Bsp1407I TGTACA 1 cut(s) 199
Bsp143I GATC 4 cut(s) 78, 570, 920, 964
BspACI CCGC 6 cut(s) 6, 9, 30, 906, 984, 1058
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 370, 981
BspLI GGNNCC 4 cut(s) 648, 703, 866, 981
BspMAI CTGCAG 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 646
BspTNI GGTCTC 1 cut(s) 670
BsrBI CCGCTC 2 cut(s) 11, 986
BsrFI RCCGGY 1 cut(s) 683
BsrGI TGTACA 1 cut(s) 199
BsrI ACTGG 3 cut(s) 257, 335, 868
BssAI RCCGGY 1 cut(s) 683
BssMI GATC 4 cut(s) 78, 570, 920, 964
Bst4CI ACNGT 6 cut(s) 440, 547, 751, 916, 1073, 1081
BstAUI TGTACA 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 3 cut(s) 357, 974, 989
BstENI CCTNNNNNAGG 1 cut(s) 131
BstF5I GGATG 1 cut(s) 325
BstH2I RGCGCY 1 cut(s) 726
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 4 cut(s) 81, 573, 923, 967
BstMAI GTCTC 2 cut(s) 446, 670
BstMBI GATC 4 cut(s) 78, 570, 920, 964
BstMWI GCNNNNNNNGC 3 cut(s) 362, 371, 527
BstSCI CCNGG 1 cut(s) 642
BstSFI CTRYAG 1 cut(s) 60
BstV1I GCAGC 5 cut(s) 286, 365, 383, 410, 732
BsuI GTATCC 1 cut(s) 1095
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 325
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 6 cut(s) 342, 552, 744, 816, 875, 1086
Cac8I GCNNGC 1 cut(s) 46
CaiI CAGNNNCTG 1 cut(s) 407
CfoI GCGC 1 cut(s) 725
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 1 cut(s) 590
CseI GACGC 1 cut(s) 1020
Csp6I GTAC 1 cut(s) 200
CspAI ACCGGT 1 cut(s) 683
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviAII CATG 7 cut(s) 203, 412, 445, 518, 780, 949, 1028
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 3 cut(s) 357, 974, 989
DpnI GATC 4 cut(s) 80, 572, 922, 966
DpnII GATC 4 cut(s) 78, 570, 920, 964
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 598
Eco24I GRGCYC 3 cut(s) 600, 704, 982
Eco31I GGTCTC 1 cut(s) 670
Eco32I GATATC 1 cut(s) 776
Eco47I GGWCC 1 cut(s) 590
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 3 cut(s) 135, 159, 1032
Eco88I CYCGRG 1 cut(s) 790
EcoICRI GAGCTC 1 cut(s) 598
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoRV GATATC 1 cut(s) 776
EcoT38I GRGCYC 3 cut(s) 600, 704, 982
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 7 cut(s) 206, 415, 448, 521, 783, 952, 1031
FaqI GGGAC 1 cut(s) 556
FatI CATG 7 cut(s) 202, 411, 444, 517, 779, 948, 1027
FauI CCCGC 1 cut(s) 991
FauNDI CATATG 1 cut(s) 367
Fnu4HI GCNGC 8 cut(s) 6, 9, 275, 354, 372, 399, 721, 906
FokI GGATG 1 cut(s) 332
FriOI GRGCYC 3 cut(s) 600, 704, 982
Fsp4HI GCNGC 8 cut(s) 6, 9, 275, 354, 372, 399, 721, 906
FspBI CTAG 4 cut(s) 216, 297, 306, 509
GlaI GCGC 1 cut(s) 724
GluI GCNGC 8 cut(s) 6, 9, 275, 354, 372, 399, 721, 906
GsaI CCCAGC 1 cut(s) 701
HaeII RGCGCY 1 cut(s) 726
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 644, 684
HgaI GACGC 1 cut(s) 1020
HhaI GCGC 1 cut(s) 725
Hin1II CATG 7 cut(s) 206, 415, 448, 521, 783, 952, 1031
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HincII GTYRAC 3 cut(s) 504, 612, 622
HindII GTYRAC 3 cut(s) 504, 612, 622
HindIII AAGCTT 2 cut(s) 533, 893
HinfI GANTC 2 cut(s) 404, 823
HpaII CCGG 2 cut(s) 644, 684
HphI GGTGA 2 cut(s) 19, 645
Hpy166II GTNNAC 4 cut(s) 504, 612, 622, 1024
Hpy188I TCNGA 5 cut(s) 184, 360, 990, 1051, 1089
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 4 cut(s) 504, 612, 622, 1024
HpyAV CCTTC 8 cut(s) 59, 159, 221, 520, 589, 635, 666, 715
HpyCH4III ACNGT 6 cut(s) 440, 547, 751, 916, 1073, 1081
HpyCH4IV ACGT 1 cut(s) 618
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 371, 527
HpyF3I CTNAG 3 cut(s) 357, 974, 989
HpySE526I ACGT 1 cut(s) 618
Hsp92II CATG 7 cut(s) 206, 415, 448, 521, 783, 952, 1031
HspAI GCGC 1 cut(s) 723
Kzo9I GATC 4 cut(s) 78, 570, 920, 964
LmnI GCTCC 4 cut(s) 16, 707, 864, 985
Lsp1109I GCAGC 5 cut(s) 286, 365, 383, 410, 732
MaeI CTAG 4 cut(s) 216, 297, 306, 509
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 1 cut(s) 689
MalI GATC 4 cut(s) 80, 572, 922, 966
MbiI CCGCTC 2 cut(s) 11, 986
MboI GATC 4 cut(s) 78, 570, 920, 964
MboII GAAGA 4 cut(s) 152, 574, 620, 1038
MhlI GDGCHC 4 cut(s) 600, 704, 797, 982
MluCI AATT 1 cut(s) 121
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 2 cut(s) 474, 863
MnlI CCTC 9 cut(s) 24, 124, 127, 428, 463, 480, 626, 849, 981
MseI TTAA 2 cut(s) 326, 933
MspI CCGG 2 cut(s) 644, 684
MspR9I CCNGG 1 cut(s) 644
MwoI GCNNNNNNNGC 3 cut(s) 362, 371, 527
NciI CCSGG 1 cut(s) 644
NdeI CATATG 1 cut(s) 367
NdeII GATC 4 cut(s) 78, 570, 920, 964
NlaIII CATG 7 cut(s) 206, 415, 448, 521, 783, 952, 1031
NlaIV GGNNCC 4 cut(s) 648, 703, 866, 981
NmeAIII GCCGAG 2 cut(s) 689, 696
NmuCI GTSAC 1 cut(s) 689
PaeR7I CTCGAG 1 cut(s) 790
PcsI WCGNNNNNNNCGW 1 cut(s) 723
PfeI GAWTC 1 cut(s) 823
PinAI ACCGGT 1 cut(s) 683
PkrI GCNGC 8 cut(s) 7, 10, 276, 355, 373, 400, 722, 907
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
PshBI ATTAAT 1 cut(s) 326
Psp124BI GAGCTC 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 697
PspN4I GGNNCC 4 cut(s) 648, 703, 866, 981
PspPI GGNCC 1 cut(s) 590
PspXI VCTCGAGB 1 cut(s) 790
PstI CTGCAG 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 407
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 2 cut(s) 326, 933
SatI GCNGC 8 cut(s) 6, 9, 275, 354, 372, 399, 721, 906
Sau3AI GATC 4 cut(s) 78, 570, 920, 964
Sau96I GGNCC 1 cut(s) 590
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 1 cut(s) 644
SduI GDGCHC 4 cut(s) 600, 704, 797, 982
SfcI CTRYAG 1 cut(s) 60
Sfr274I CTCGAG 1 cut(s) 790
SinI GGWCC 1 cut(s) 590
SlaI CTCGAG 1 cut(s) 790
SmlI CTYRAG 3 cut(s) 90, 128, 790
SmoI CTYRAG 3 cut(s) 90, 128, 790
Sse9I AATT 1 cut(s) 121
SsiI CCGC 6 cut(s) 6, 9, 30, 906, 984, 1058
SspI AATATT 1 cut(s) 424
SspMI CTAG 4 cut(s) 216, 297, 306, 509
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 1 cut(s) 642
TaaI ACNGT 6 cut(s) 440, 547, 751, 916, 1073, 1081
TaiI ACGT 1 cut(s) 621
TaqI TCGA 6 cut(s) 69, 486, 631, 791, 821, 963
TaqII GACCGA 1 cut(s) 652
TasI AATT 1 cut(s) 121
TatI WGTACW 1 cut(s) 199
TauI GCSGC 3 cut(s) 8, 11, 908
TfiI GAWTC 1 cut(s) 823
Tru1I TTAA 2 cut(s) 326, 933
Tru9I TTAA 2 cut(s) 326, 933
TscAI CASTG 6 cut(s) 342, 552, 744, 816, 875, 1086
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 5 cut(s) 274, 353, 371, 398, 720
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 3 cut(s) 344, 428, 648
TspRI CASTG 6 cut(s) 342, 552, 744, 816, 875, 1086
VpaK11BI GGWCC 1 cut(s) 590
VspI ATTAAT 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 131
XhoI CTCGAG 1 cut(s) 790
XspI CTAG 4 cut(s) 216, 297, 306, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.