MD08G1224400.v1.1

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000337
Physical Location & Seq
Forward (+)
28924771 .. 28925894
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1224400.v1.1.491

Sequence Viewer

Length: 693 bp
ATGAAGTTAGCCATTGGGACACGCACGGGAAGGGCAAAAGTTGTGGGTACATTAGTTTGTGTAGGAGGCACAATGATCTTCACTTTCTACAAAGGCCACGAGATTGTCATTTGGTTGACGGACGTAAACCTTTTACGTAAATATTCCCACCAAAACAGCCACGTCTTGCACAGAAACACTCGCACTCAGTTGCTGGGCTCCGTCTTAGCACTCTGCAGTAGCATCTCTTTTGCAATGTGGCTGATATTTCAGAAGAAAATGACTAGGGATTACCCTTGCCAGTACTCAAGTACGGCCCTGATGTCTATCATGGCATCAATCCAGTCCACTGCTTTTGGCCTCTGCAAGGAGAGGGACTGGAGTGAGTGGAAGTTGGGCCGGGACATCAAACTTTTCACGGCAGTGTATTCGGGAGTCATTACTTCGGGATTGGTCCTTACTCTGACCGCATGGTGTGTGCGCATGCGAGGGCCTTTGTTTGTGGCTATTTTCAACCCTCTTGGCCTTCTGCTGGTGGGCTTAGCTGCGACGTTGTTGTTAGATGAGAAGTTACACTTAGGAAACATATTAGGAGAGTTTTTAATCATCTGTGGGTTATATATGGTTTTGTGGAGTAAAAGAGAGGAACTGAAGACGATGACAGAACAGCCACCATCACTACTTGAAGAGTCCCATTCAGAGAGGTTCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

231

Amino Acids

25.9

Weight (kDa)

9.23

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 65 - 203 2.6e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 461
AciI CCGC 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 650
AfaI GTAC 3 cut(s) 49, 284, 292
AfiI CCNNNNNNNGG 2 cut(s) 346, 511
AgsI TTSAA 2 cut(s) 493, 665
AjiI CACGTC 1 cut(s) 163
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 1 cut(s) 524
AluI AGCT 1 cut(s) 524
AlwNI CAGNNNCTG 1 cut(s) 193
AoxI GGCC 6 cut(s) 94, 294, 337, 376, 470, 502
ApeKI GCWGC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 4 cut(s) 295, 376, 433, 470
AsuC2I CCSGG 1 cut(s) 380
AvaII GGWCC 1 cut(s) 433
BaeI ACNNNNGTAYC 2 cut(s) 39, 72
BanII GRGCYC 1 cut(s) 200
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 638
BbvI GCAGC 1 cut(s) 511
BccI CCATC 1 cut(s) 661
BceAI ACGGC 2 cut(s) 309, 414
BcgI CGANNNNNNTGC 2 cut(s) 390, 424
BcnI CCSGG 1 cut(s) 380
BfaI CTAG 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 214
BisI GCNGC 1 cut(s) 525
BlpI GCTNAGC 1 cut(s) 520
BlsI GCNGC 1 cut(s) 526
BmcAI AGTACT 1 cut(s) 284
Bme1390I CCNGG 1 cut(s) 380
Bme18I GGWCC 1 cut(s) 433
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 4 cut(s) 295, 376, 433, 470
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 380
BmsI GCATC 2 cut(s) 231, 323
BpiI GAAGAC 1 cut(s) 638
BpmI CTGGAG 1 cut(s) 379
Bpu1102I GCTNAGC 1 cut(s) 520
BpuEI CTTGAG 1 cut(s) 271
BpuMI CCSGG 1 cut(s) 380
BsaAI YACGTR 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 305
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 511
Bse1I ACTGG 3 cut(s) 280, 322, 362
Bse3DI GCAATG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 305
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 2 cut(s) 346, 511
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 3 cut(s) 280, 322, 362
BseXI GCAGC 1 cut(s) 511
BseYI CCCAGC 1 cut(s) 193
BshFI GGCC 6 cut(s) 96, 296, 339, 378, 472, 504
BsiSI CCGG 1 cut(s) 379
BslFI GGGAC 4 cut(s) 31, 368, 395, 655
BslI CCNNNNNNNGG 2 cut(s) 346, 511
BsmFI GGGAC 4 cut(s) 31, 368, 395, 655
BsnI GGCC 6 cut(s) 96, 296, 339, 378, 472, 504
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 1 cut(s) 75
Bsp1720I GCTNAGC 1 cut(s) 520
BspACI CCGC 1 cut(s) 447
BspANI GGCC 6 cut(s) 96, 296, 339, 378, 472, 504
BspCNI CTCAG 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 199
BspMAI CTGCAG 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 240
BsrI ACTGG 3 cut(s) 280, 322, 362
BssMI GATC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 98
Bst2BI CACGAG 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 660
BstBAI YACGTR 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 464
BstDEI CTNAG 4 cut(s) 186, 205, 520, 556
BstENI CCTNNNNNAGG 1 cut(s) 344
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 1 cut(s) 78
BstMBI GATC 1 cut(s) 75
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 1 cut(s) 378
BstSFI CTRYAG 1 cut(s) 214
BstSNI TACGTA 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 1 cut(s) 638
BsuRI GGCC 6 cut(s) 96, 296, 339, 378, 472, 504
BtrI CACGTC 1 cut(s) 163
BtsI GCAGTG 2 cut(s) 327, 408
BtsIMutI CAGTG 2 cut(s) 327, 408
Cac8I GCNNGC 1 cut(s) 464
CaiI CAGNNNCTG 1 cut(s) 193
CfoI GCGC 1 cut(s) 462
Cfr13I GGNCC 4 cut(s) 295, 376, 433, 470
Csp6I GTAC 3 cut(s) 48, 283, 291
CspCI CAANNNNNGTGG 4 cut(s) 24, 59, 316, 351
CviAII CATG 3 cut(s) 310, 450, 463
CviQI GTAC 3 cut(s) 48, 283, 291
DdeI CTNAG 4 cut(s) 186, 205, 520, 556
DpnI GATC 1 cut(s) 77
DpnII GATC 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco105I TACGTA 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 650
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 470
EcoT38I GRGCYC 1 cut(s) 200
FaeI CATG 3 cut(s) 313, 453, 466
FaiI YATR 7 cut(s) 311, 451, 464, 566, 598, 600, 602
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FaqI GGGAC 4 cut(s) 31, 368, 395, 655
FatI CATG 3 cut(s) 309, 449, 462
Fnu4HI GCNGC 1 cut(s) 525
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 1 cut(s) 525
FspAI RTGCGCAY 1 cut(s) 461
FspBI CTAG 1 cut(s) 264
FspI TGCGCA 1 cut(s) 461
GlaI GCGC 1 cut(s) 461
GluI GCNGC 1 cut(s) 525
GsaI CCCAGC 1 cut(s) 197
GsuI CTGGAG 1 cut(s) 379
HaeIII GGCC 6 cut(s) 96, 296, 339, 378, 472, 504
HapII CCGG 1 cut(s) 379
HhaI GCGC 1 cut(s) 462
Hin1II CATG 3 cut(s) 313, 453, 466
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HinfI GANTC 2 cut(s) 414, 668
HpaII CCGG 1 cut(s) 379
Hpy166II GTNNAC 3 cut(s) 117, 127, 327
Hpy188I TCNGA 3 cut(s) 252, 444, 679
Hpy188III TCNNGA 2 cut(s) 411, 426
Hpy8I GTNNAC 3 cut(s) 117, 127, 327
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 2 cut(s) 24, 515
HpyCH4IV ACGT 4 cut(s) 123, 136, 162, 530
HpyCH4V TGCA 4 cut(s) 169, 216, 233, 345
HpyF3I CTNAG 4 cut(s) 186, 205, 520, 556
HpySE526I ACGT 4 cut(s) 123, 136, 162, 530
Hsp92II CATG 3 cut(s) 313, 453, 466
HspAI GCGC 1 cut(s) 460
Kzo9I GATC 1 cut(s) 75
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 7 cut(s) 179, 293, 311, 335, 343, 392, 497
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 2 cut(s) 231, 323
MaeI CTAG 1 cut(s) 264
MaeII ACGT 4 cut(s) 123, 136, 162, 530
MaeIII GTNAC 1 cut(s) 549
MalI GATC 1 cut(s) 77
MboI GATC 1 cut(s) 75
MboII GAAGA 4 cut(s) 70, 265, 643, 677
MhlI GDGCHC 1 cut(s) 200
MlyI GAGTC 2 cut(s) 423, 677
MnlI CCTC 7 cut(s) 59, 345, 350, 461, 507, 616, 675
MseI TTAA 2 cut(s) 581, 691
MslI CAYNNNNRTG 1 cut(s) 401
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 1 cut(s) 380
NciI CCSGG 1 cut(s) 380
NdeII GATC 1 cut(s) 75
NlaIII CATG 3 cut(s) 313, 453, 466
NlaIV GGNNCC 1 cut(s) 199
NsbI TGCGCA 1 cut(s) 461
NspI RCATGY 1 cut(s) 466
OliI CACNNNNGTG 1 cut(s) 401
PaeI GCATGC 1 cut(s) 466
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 2 cut(s) 422, 676
PpsI GAGTC 2 cut(s) 422, 676
Ppu21I YACGTR 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 199
PspPI GGNCC 4 cut(s) 295, 376, 433, 470
PstI CTGCAG 1 cut(s) 218
PstNI CAGNNNCTG 1 cut(s) 193
RsaI GTAC 3 cut(s) 49, 284, 292
RsaNI GTAC 3 cut(s) 48, 283, 291
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 2 cut(s) 581, 691
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 1 cut(s) 75
Sau96I GGNCC 4 cut(s) 295, 376, 433, 470
ScaI AGTACT 1 cut(s) 284
SchI GAGTC 2 cut(s) 423, 677
ScrFI CCNGG 1 cut(s) 380
SduI GDGCHC 1 cut(s) 200
SetI ASST 7 cut(s) 126, 132, 139, 165, 526, 533, 686
SfaNI GCATC 2 cut(s) 231, 323
SfcI CTRYAG 1 cut(s) 214
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
SnaBI TACGTA 1 cut(s) 137
SphI GCATGC 1 cut(s) 466
SsiI CCGC 1 cut(s) 447
SspI AATATT 1 cut(s) 143
SspMI CTAG 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 378
TaiI ACGT 4 cut(s) 126, 139, 165, 533
TatI WGTACW 1 cut(s) 282
Tru1I TTAA 2 cut(s) 581, 691
Tru9I TTAA 2 cut(s) 581, 691
TscAI CASTG 2 cut(s) 334, 408
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 134, 190
TspRI CASTG 2 cut(s) 334, 408
VpaK11BI GGWCC 1 cut(s) 433
XagI CCTNNNNNAGG 1 cut(s) 344
XceI RCATGY 1 cut(s) 466
XspI CTAG 1 cut(s) 264
ZrmI AGTACT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.