Rroxscaffold_3G00234560

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000337
Physical Location & Seq
Forward (+)
21031546 .. 21033267
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00234560.1

Sequence Viewer

Length: 990 bp
ATGGGGAAGCAGAGTAGTGTAGTTGATGGCTTAATGAATCCAGTGATAACGATGGTGATCGCTCAGATTGCATATGCAGCAACGAGTATATTCTACAAAGTTGCAGCAGACTCTGGAATGAATCTCAGGATTCTCGTTGCCTACCGTCTCATGTTTTCATCTGCCATTATCGTTCCACTTGCTCTTAGACTCGAAAGGAACAGCAGGCCCAAACTCACTCTAGTCGTACTCTTTCAAGCTTTTCTCTGCGGATTATTTGGGGGATCATTGTCAGAAAATTTATTCATACAGGGATTAGTCGTAACATCACCAACCTTTGTTGCAGCCACTGCAAATCTGATTCCAGCCGTCACCTTCATCATGGCCATCTCTTTCAGGTTGGAGAAGTTGGCACTAGGAAGCCATGCAGGGAAGGCAAAGCTAGTGGGGACAGTGGCGGGTATTGGTGGTGCTATGATCTTTACCTTCTTCAAAGGACCAGAGTTCTCCATCTGGTCAATTCACGTTGACCTTCTTCGAGGACATCAGGCGGCAACATCACCGTCATCGTCCTCCTCCCACAGAATCACCGGTAGTCACCTGCTGGGCTCCTTCTTGGCACTCGGCTCCGTCGTTTCCTATGCAATGTGGCTCATAGTTCAGGCAAAAATGAGCAAGAGATATCCATGCCATTACTCGAGCACGGCTCTATTGTCGGTGATGGGATCGATTCAGTCTGTAGCTTTTGCTCTCTGCGTGGAGAGGGATTGGAGCCAGTGGAAATTGGGTTGGAACATCAAGCTTTTTACAGCTGCATATGCGGGAATTGTGACTTCGGGGATGGTGGTGGTTTTGATCTCATGGTGTGTACGCAAGCGAGGTCCATTGTTTGTGTCAGTTTTCACTCCGCTTTCCCTAGTGATCGTGGCCATTGCTAGTTCTTTATTTCTCAATGATAAGTTAAGCCTAGGAAATGCTCTGGGGGAAGAGCGAAGAGATGAAGACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

329

Amino Acids

35.45

Weight (kDa)

9.69

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 15 - 154 2.5e-10 EamA-like transporter family
EamA PF00892 195 - 321 4.6e-10 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 588
Acc36I ACCTGC 1 cut(s) 588
AciI CCGC 5 cut(s) 249, 437, 530, 800, 887
AclWI GGATC 2 cut(s) 271, 712
AcoI YGGCCR 2 cut(s) 363, 906
AcsI RAATTY 1 cut(s) 277
AfaI GTAC 2 cut(s) 228, 849
AgeI ACCGGT 1 cut(s) 569
AgsI TTSAA 2 cut(s) 236, 472
AluBI AGCT 5 cut(s) 239, 421, 722, 781, 791
AluI AGCT 5 cut(s) 239, 421, 722, 781, 791
Alw21I GWGCWC 1 cut(s) 683
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 2 cut(s) 271, 712
AlwNI CAGNNNCTG 2 cut(s) 113, 329
Ama87I CYCGRG 1 cut(s) 676
AoxI GGCC 3 cut(s) 206, 363, 906
ApeKI GCWGC 4 cut(s) 77, 104, 323, 791
ApoI RAATTY 1 cut(s) 277
AsiGI ACCGGT 1 cut(s) 569
AspA2I CCTAGG 1 cut(s) 946
AspS9I GGNCC 3 cut(s) 207, 476, 860
AsuHPI GGTGA 7 cut(s) 67, 300, 343, 531, 559, 569, 709
AvaI CYCGRG 1 cut(s) 676
AvaII GGWCC 2 cut(s) 476, 860
AvrII CCTAGG 1 cut(s) 946
BalI TGGCCA 2 cut(s) 365, 908
BanII GRGCYC 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 683
BbvI GCAGC 4 cut(s) 89, 116, 335, 778
BccI CCATC 6 cut(s) 20, 46, 374, 497, 694, 814
BceAI ACGGC 2 cut(s) 332, 699
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 6 cut(s) 221, 395, 422, 896, 915, 947
BfmI CTRYAG 1 cut(s) 717
BfuAI ACCTGC 1 cut(s) 588
BisI GCNGC 5 cut(s) 78, 105, 324, 531, 792
BlnI CCTAGG 1 cut(s) 946
BlsI GCNGC 5 cut(s) 79, 106, 325, 532, 793
Bme18I GGWCC 2 cut(s) 476, 860
BmeT110I CYCGRG 1 cut(s) 676
BmgT120I GGNCC 3 cut(s) 207, 476, 860
BmiI GGNNCC 3 cut(s) 589, 607, 752
BplI GAGNNNNNCTC 2 cut(s) 670, 702
Bsa29I ATCGAT 1 cut(s) 707
BsaBI GATNNNNATC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 946
BsaWI WCCGGW 1 cut(s) 569
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 2 cut(s) 41, 754
Bse3DI GCAATG 2 cut(s) 630, 909
Bse8I GATNNNNATC 1 cut(s) 56
BseCI ATCGAT 1 cut(s) 707
BseDI CCNNGG 1 cut(s) 946
BseGI GGATG 1 cut(s) 825
BseJI GATNNNNATC 1 cut(s) 56
BseMI GCAATG 2 cut(s) 630, 909
BseMII CTCAG 2 cut(s) 77, 139
BseNI ACTGG 2 cut(s) 41, 754
BseRI GAGGAG 1 cut(s) 544
BseXI GCAGC 4 cut(s) 89, 116, 335, 778
BseYI CCCAGC 1 cut(s) 583
BshFI GGCC 3 cut(s) 208, 365, 908
BshTI ACCGGT 1 cut(s) 569
BshVI ATCGAT 1 cut(s) 707
BsiHKAI GWGCWC 1 cut(s) 683
BsiHKCI CYCGRG 1 cut(s) 676
BsiSI CCGG 1 cut(s) 570
BslFI GGGAC 1 cut(s) 442
BsmAI GTCTC 1 cut(s) 152
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 3 cut(s) 208, 365, 908
BsoBI CYCGRG 1 cut(s) 676
Bsp1286I GDGCHC 2 cut(s) 590, 683
Bsp143I GATC 6 cut(s) 57, 263, 456, 704, 834, 900
BspACI CCGC 5 cut(s) 249, 437, 530, 800, 887
BspANI GGCC 3 cut(s) 208, 365, 908
BspCNI CTCAG 2 cut(s) 76, 138
BspDI ATCGAT 1 cut(s) 707
BspLI GGNNCC 3 cut(s) 589, 607, 752
BspMI ACCTGC 1 cut(s) 588
BspPI GGATC 2 cut(s) 271, 712
BspQI GCTCTTC 1 cut(s) 960
BsrDI GCAATG 2 cut(s) 630, 909
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 2 cut(s) 41, 754
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 1 cut(s) 946
BssMI GATC 6 cut(s) 57, 263, 456, 704, 834, 900
BssT1I CCWWGG 1 cut(s) 946
Bst4CI ACNGT 3 cut(s) 146, 433, 543
Bst6I CTCTTC 2 cut(s) 960, 967
BstAPI GCANNNNNTGC 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 206, 854
BstDEI CTNAG 3 cut(s) 63, 125, 185
BstF5I GGATG 1 cut(s) 825
BstKTI GATC 6 cut(s) 60, 266, 459, 707, 837, 903
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 6 cut(s) 57, 263, 456, 704, 834, 900
BstMWI GCNNNNNNNGC 5 cut(s) 68, 77, 329, 413, 797
BstSFI CTRYAG 1 cut(s) 717
BstV1I GCAGC 4 cut(s) 89, 116, 335, 778
Bsu15I ATCGAT 1 cut(s) 707
BsuRI GGCC 3 cut(s) 208, 365, 908
BsuTUI ATCGAT 1 cut(s) 707
BtsCI GGATG 1 cut(s) 825
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 4 cut(s) 48, 327, 438, 761
BveI ACCTGC 1 cut(s) 588
Cac8I GCNNGC 2 cut(s) 206, 854
CaiI CAGNNNCTG 2 cut(s) 113, 329
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 3 cut(s) 207, 476, 860
ClaI ATCGAT 1 cut(s) 707
Csp6I GTAC 2 cut(s) 227, 848
CspAI ACCGGT 1 cut(s) 569
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 5 cut(s) 151, 361, 404, 666, 840
CviQI GTAC 2 cut(s) 227, 848
DdeI CTNAG 3 cut(s) 63, 125, 185
DpnI GATC 6 cut(s) 59, 265, 458, 706, 836, 902
DpnII GATC 6 cut(s) 57, 263, 456, 704, 834, 900
EaeI YGGCCR 2 cut(s) 363, 906
Eam1104I CTCTTC 2 cut(s) 960, 967
EarI CTCTTC 2 cut(s) 960, 967
Eco130I CCWWGG 1 cut(s) 946
Eco24I GRGCYC 1 cut(s) 590
Eco32I GATATC 1 cut(s) 662
Eco47I GGWCC 2 cut(s) 476, 860
Eco88I CYCGRG 1 cut(s) 676
EcoRV GATATC 1 cut(s) 662
EcoT14I CCWWGG 1 cut(s) 946
EcoT38I GRGCYC 1 cut(s) 590
ErhI CCWWGG 1 cut(s) 946
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 5 cut(s) 154, 364, 407, 669, 843
FaqI GGGAC 1 cut(s) 442
FatI CATG 5 cut(s) 150, 360, 403, 665, 839
FauI CCCGC 2 cut(s) 430, 793
FauNDI CATATG 2 cut(s) 73, 796
Fnu4HI GCNGC 5 cut(s) 78, 105, 324, 531, 792
FokI GGATG 1 cut(s) 832
FriOI GRGCYC 1 cut(s) 590
Fsp4HI GCNGC 5 cut(s) 78, 105, 324, 531, 792
FspBI CTAG 6 cut(s) 221, 395, 422, 896, 915, 947
GluI GCNGC 5 cut(s) 78, 105, 324, 531, 792
GsaI CCCAGC 1 cut(s) 587
HaeIII GGCC 3 cut(s) 208, 365, 908
HapII CCGG 1 cut(s) 570
Hin1II CATG 5 cut(s) 154, 364, 407, 669, 843
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 2 cut(s) 237, 779
HinfI GANTC 8 cut(s) 37, 110, 121, 130, 189, 340, 564, 709
HpaII CCGG 1 cut(s) 570
HphI GGTGA 7 cut(s) 67, 300, 343, 531, 559, 569, 709
Hpy166II GTNNAC 2 cut(s) 508, 848
Hpy188I TCNGA 3 cut(s) 66, 274, 339
Hpy188III TCNNGA 2 cut(s) 114, 127
Hpy8I GTNNAC 2 cut(s) 508, 848
Hpy99I CGWCG 1 cut(s) 614
HpyAV CCTTC 5 cut(s) 364, 406, 475, 521, 601
HpyCH4III ACNGT 3 cut(s) 146, 433, 543
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 8 cut(s) 71, 77, 104, 323, 332, 407, 623, 794
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 77, 329, 413, 797
HpyF3I CTNAG 3 cut(s) 63, 125, 185
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 5 cut(s) 154, 364, 407, 669, 843
Kzo9I GATC 6 cut(s) 57, 263, 456, 704, 834, 900
LguI GCTCTTC 1 cut(s) 960
LmnI GCTCC 3 cut(s) 593, 611, 750
Lsp1109I GCAGC 4 cut(s) 89, 116, 335, 778
MaeI CTAG 6 cut(s) 221, 395, 422, 896, 915, 947
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 4 cut(s) 301, 349, 575, 808
MalI GATC 6 cut(s) 59, 265, 458, 706, 836, 902
MboI GATC 6 cut(s) 57, 263, 456, 704, 834, 900
MboII GAAGA 4 cut(s) 460, 506, 977, 984
MhlI GDGCHC 2 cut(s) 590, 683
MlsI TGGCCA 2 cut(s) 365, 908
MluCI AATT 4 cut(s) 277, 498, 761, 804
MluNI TGGCCA 2 cut(s) 365, 908
MlyI GAGTC 2 cut(s) 104, 183
MmeI TCCRAC 2 cut(s) 360, 749
MnlI CCTC 5 cut(s) 512, 562, 565, 735, 851
Mox20I TGGCCA 2 cut(s) 365, 908
MscI TGGCCA 2 cut(s) 365, 908
MseI TTAA 3 cut(s) 32, 941, 988
Msp20I TGGCCA 2 cut(s) 365, 908
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 1 cut(s) 570
MwoI GCNNNNNNNGC 5 cut(s) 68, 77, 329, 413, 797
NdeI CATATG 2 cut(s) 73, 796
NdeII GATC 6 cut(s) 57, 263, 456, 704, 834, 900
NlaIII CATG 5 cut(s) 154, 364, 407, 669, 843
NlaIV GGNNCC 3 cut(s) 589, 607, 752
NmeAIII GCCGAG 1 cut(s) 582
NmuCI GTSAC 3 cut(s) 349, 575, 808
PaeR7I CTCGAG 1 cut(s) 676
PaqCI CACCTGC 1 cut(s) 588
PciSI GCTCTTC 1 cut(s) 960
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PfeI GAWTC 6 cut(s) 37, 121, 130, 340, 564, 709
PinAI ACCGGT 1 cut(s) 569
PkrI GCNGC 5 cut(s) 79, 106, 325, 532, 793
PleI GAGTC 2 cut(s) 104, 183
PpsI GAGTC 2 cut(s) 104, 183
PspFI CCCAGC 1 cut(s) 583
PspN4I GGNNCC 3 cut(s) 589, 607, 752
PspPI GGNCC 3 cut(s) 207, 476, 860
PspXI VCTCGAGB 1 cut(s) 676
PstNI CAGNNNCTG 2 cut(s) 113, 329
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 2 cut(s) 228, 849
RsaNI GTAC 2 cut(s) 227, 848
SapI GCTCTTC 1 cut(s) 960
SaqAI TTAA 3 cut(s) 32, 941, 988
SatI GCNGC 5 cut(s) 78, 105, 324, 531, 792
Sau3AI GATC 6 cut(s) 57, 263, 456, 704, 834, 900
Sau96I GGNCC 3 cut(s) 207, 476, 860
SchI GAGTC 2 cut(s) 104, 183
SduI GDGCHC 2 cut(s) 590, 683
SfcI CTRYAG 1 cut(s) 717
Sfr274I CTCGAG 1 cut(s) 676
SinI GGWCC 2 cut(s) 476, 860
SlaI CTCGAG 1 cut(s) 676
SmlI CTYRAG 1 cut(s) 676
SmoI CTYRAG 1 cut(s) 676
Sse9I AATT 4 cut(s) 277, 498, 761, 804
SsiI CCGC 5 cut(s) 249, 437, 530, 800, 887
SspMI CTAG 6 cut(s) 221, 395, 422, 896, 915, 947
StyI CCWWGG 1 cut(s) 946
TaaI ACNGT 3 cut(s) 146, 433, 543
TaiI ACGT 1 cut(s) 507
TaqI TCGA 4 cut(s) 192, 517, 677, 707
TasI AATT 4 cut(s) 277, 498, 761, 804
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 6 cut(s) 37, 121, 130, 340, 564, 709
Tru1I TTAA 3 cut(s) 32, 941, 988
Tru9I TTAA 3 cut(s) 32, 941, 988
TscAI CASTG 4 cut(s) 48, 334, 438, 761
TseFI GTSAC 3 cut(s) 349, 575, 808
TseI GCWGC 4 cut(s) 77, 104, 323, 791
Tsp45I GTSAC 3 cut(s) 349, 575, 808
TspDTI ATGAA 5 cut(s) 50, 134, 147, 274, 346
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 4 cut(s) 48, 334, 438, 761
VpaK11BI GGWCC 2 cut(s) 476, 860
XapI RAATTY 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 676
XmaJI CCTAGG 1 cut(s) 946
XspI CTAG 6 cut(s) 221, 395, 422, 896, 915, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.