Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0000337

Physical Location & Seq

Genomic Coordinates Forward (+)

20657652 .. 20660972

Gene Structure 3321 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00234350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1116 bp

ATGGGTGGTAGTTGTTTTAATGCTTTGCATGGGTTGAAGCCATTGATGTTAATGCTGGTGGTTCAGTTTACATCTGCTGGAGTCAACTTGTTCTACAAACTGGCCGCTAATGATGGAATGGACATCAGGATTATTATTGCCTATCGCTTCAGTTTTGCCACAGTTTTCCTTGCTCCTATTGCTCTCTTCGTTGAAAGGGGAGCATTGTCACAAAATTTGTACATTGCAAGTTTAGCGTTAACGTCTGCAACATTTGCTCTCGCCATAAGTCAACTCATCCCGGCCATAACTTTTGTTTTGGCCATCTCCTTTGGGTACGTTGGAGAGATTGAATTTAAGGAGCGTAGGGGGAAGGCAAAGGTGTTGGGAACATTAATGGGAATAGGTGGAGCAATGCTTATGACCTTTTACAAAGGTCTGGAAATCCACTTATGGACCACCCATGTTGACCTTTTGCATAGTAGTCATCAGCAACAACCACACTTGGAGGCAGCAGCTTCGCATGAGTATGCTCATCGTCTGTTAGGTTGTCTTCTGGCTCTGGCCAGCTCCTTGGGTTACGCCTTGTGGCTAATTATTCAGGCTAAGATGGGTGAGAAATACCCCAGTTTTTATTCAAGCGCAGCTCTAATTTCACTTATGGGGTCAATCCAGTCCCTTGTTTTTGCCCTCTGCGTCGACAGGGACTGGAACCATTGGAAGTTGGGTTGGAATATTAGACTTCTAACAGTCTTATACCTGGGGACTGTGGGTACTGGAGTAAGTCTGACATGTACTACACTTTGCATAAGCATGAGAGGGCCTTTGTACGCGTCAGTTTTTGGGCCTTTGGTTCTTGTTCTTGTTGCCCTCGTTGGCTCTTTGGTGTTGGACGAGAAGCTGCACCTCGGAAGTGTGCTAGGAGCGGTTCTAATCGTTGGCGGCTTATATGCTGTTCTATGGGGAAAGAGCAAAGACATAAAGAAGGTGAATCAATCGGTGCCAACAACAAGCTATGAGGAGGAATCTGCAGGATCTGTAGTTGAAATTGTTTCAATGCCTCGGGATAATAATAAAGGCAATAACTACATGAATGAGACCGTCACAAATGGTCAACATGACCGTATAGAAACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

371

Amino Acids

40.32

Weight (kDa)

8.2

Isoelectric Point (pI)

31.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	175 - 313	1.1e-12	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25270 AT1G68170 AT1G68170
fragaria_vesca	FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica	MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica	Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis	pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis	RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata	RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora	Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii	Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa	Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha	Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana	Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	979
AccBSI	CCGCTC	1 cut(s)	905
AccI	GTMKAC	1 cut(s)	678
AccII	CGCG	1 cut(s)	812
AciI	CCGC	3 cut(s)	105, 905, 921
AclWI	GGATC	1 cut(s)	1021
AcoI	YGGCCR	4 cut(s)	102, 282, 300, 543
AcsI	RAATTY	2 cut(s)	214, 332
AcuI	CTGAAG	1 cut(s)	133
AfaI	GTAC	5 cut(s)	221, 317, 754, 775, 809
AflIII	ACRYGT	2 cut(s)	770, 810
AgsI	TTSAA	6 cut(s)	37, 194, 332, 618, 1025, 1035
AjnI	CCWGG	1 cut(s)	738
AluBI	AGCT	5 cut(s)	497, 549, 626, 880, 993
AluI	AGCT	5 cut(s)	497, 549, 626, 880, 993
Alw26I	GTCTC	1 cut(s)	1070
AlwI	GGATC	1 cut(s)	1021
AlwNI	CAGNNNCTG	2 cut(s)	687, 1016
Ama87I	CYCGRG	1 cut(s)	1041
AoxI	GGCC	6 cut(s)	102, 282, 300, 543, 800, 824
ApeKI	GCWGC	4 cut(s)	491, 494, 623, 880
ApoI	RAATTY	2 cut(s)	214, 332
AseI	ATTAAT	1 cut(s)	374
AspLEI	GCGC	1 cut(s)	623
AspS9I	GGNCC	3 cut(s)	435, 800, 824
AsuC2I	CCSGG	1 cut(s)	281
AsuHPI	GGTGA	2 cut(s)	605, 979
AvaI	CYCGRG	1 cut(s)	1041
AvaII	GGWCC	1 cut(s)	435
BaeI	ACNNNNGTAYC	2 cut(s)	744, 777
BalI	TGGCCA	2 cut(s)	302, 545
BanI	GGYRCC	1 cut(s)	979
BbsI	GAAGAC	1 cut(s)	524
BbvI	GCAGC	4 cut(s)	503, 506, 635, 867
BccI	CCATC	3 cut(s)	107, 311, 583
BcgI	CGANNNNNNTGC	2 cut(s)	480, 514
BciT130I	CCWGG	1 cut(s)	740
BcnI	CCSGG	1 cut(s)	281
BcoDI	GTCTC	1 cut(s)	1070
BfaI	CTAG	2 cut(s)	899, 1114
BfmI	CTRYAG	2 cut(s)	1008, 1017
BisI	GCNGC	6 cut(s)	105, 492, 495, 624, 881, 922
BlsI	GCNGC	6 cut(s)	106, 493, 496, 625, 882, 923
Bme1390I	CCNGG	2 cut(s)	281, 740
Bme18I	GGWCC	1 cut(s)	435
BmeT110I	CYCGRG	1 cut(s)	1041
BmgT120I	GGNCC	3 cut(s)	435, 800, 824
BmiI	GGNNCC	2 cut(s)	692, 981
BmrFI	CCNGG	2 cut(s)	281, 740
BmrI	ACTGGG	1 cut(s)	600
BmuI	ACTGGG	1 cut(s)	600
BpiI	GAAGAC	1 cut(s)	524
BpmI	CTGGAG	2 cut(s)	99, 777
BpuMI	CCSGG	1 cut(s)	281
BsaI	GGTCTC	1 cut(s)	1070
BsaJI	CCNNGG	4 cut(s)	552, 739, 886, 1040
Bse1I	ACTGG	5 cut(s)	105, 606, 652, 692, 760
Bse3DI	GCAATG	2 cut(s)	222, 399
BseBI	CCWGG	1 cut(s)	740
BseDI	CCNNGG	4 cut(s)	552, 739, 886, 1040
BseGI	GGATG	1 cut(s)	276
BseMI	GCAATG	2 cut(s)	222, 399
BseNI	ACTGG	5 cut(s)	105, 606, 652, 692, 760
BseRI	GAGGAG	1 cut(s)	1013
BseXI	GCAGC	4 cut(s)	503, 506, 635, 867
BsgI	GTGCAG	1 cut(s)	866
Bsh1236I	CGCG	1 cut(s)	812
BshFI	GGCC	6 cut(s)	104, 284, 302, 545, 802, 826
BshNI	GGYRCC	1 cut(s)	979
BsiHKCI	CYCGRG	1 cut(s)	1041
BsiSI	CCGG	1 cut(s)	281
BslFI	GGGAC	3 cut(s)	640, 698, 757
BsmAI	GTCTC	1 cut(s)	1070
BsmFI	GGGAC	3 cut(s)	640, 698, 757
BsnI	GGCC	6 cut(s)	104, 284, 302, 545, 802, 826
Bso31I	GGTCTC	1 cut(s)	1070
BsoBI	CYCGRG	1 cut(s)	1041
Bsp1407I	TGTACA	1 cut(s)	219
Bsp143I	GATC	1 cut(s)	1013
BspACI	CCGC	3 cut(s)	105, 905, 921
BspANI	GGCC	6 cut(s)	104, 284, 302, 545, 802, 826
BspFNI	CGCG	1 cut(s)	812
BspLI	GGNNCC	2 cut(s)	692, 981
BspMAI	CTGCAG	1 cut(s)	1012
BspPI	GGATC	1 cut(s)	1021
BspT107I	GGYRCC	1 cut(s)	979
BspTNI	GGTCTC	1 cut(s)	1070
BsrBI	CCGCTC	1 cut(s)	905
BsrDI	GCAATG	2 cut(s)	222, 399
BsrGI	TGTACA	1 cut(s)	219
BsrI	ACTGG	5 cut(s)	105, 606, 652, 692, 760
BssECI	CCNNGG	4 cut(s)	552, 739, 886, 1040
BssMI	GATC	1 cut(s)	1013
BssT1I	CCWWGG	1 cut(s)	552
Bst2UI	CCWGG	1 cut(s)	740
Bst4CI	ACNGT	5 cut(s)	163, 730, 748, 1081, 1103
Bst6I	CTCTTC	1 cut(s)	191
BstAPI	GCANNNNNTGC	1 cut(s)	254
BstAUI	TGTACA	1 cut(s)	219
BstC8I	GCNNGC	1 cut(s)	547
BstDEI	CTNAG	1 cut(s)	585
BstF5I	GGATG	1 cut(s)	276
BstFNI	CGCG	1 cut(s)	812
BstHHI	GCGC	1 cut(s)	623
BstKTI	GATC	1 cut(s)	1016
BstMAI	GTCTC	1 cut(s)	1070
BstMBI	GATC	1 cut(s)	1013
BstMWI	GCNNNNNNNGC	3 cut(s)	179, 233, 254
BstNI	CCWGG	1 cut(s)	740
BstNSI	RCATGY	1 cut(s)	774
BstSCI	CCNGG	2 cut(s)	279, 738
BstSFI	CTRYAG	2 cut(s)	1008, 1017
BstUI	CGCG	1 cut(s)	812
BstV1I	GCAGC	4 cut(s)	503, 506, 635, 867
BstV2I	GAAGAC	1 cut(s)	524
BstX2I	RGATCY	1 cut(s)	1013
BstXI	CCANNNNNNTGG	1 cut(s)	553
BstYI	RGATCY	1 cut(s)	1013
BsuRI	GGCC	6 cut(s)	104, 284, 302, 545, 802, 826
BtsCI	GGATG	1 cut(s)	276
Cac8I	GCNNGC	1 cut(s)	547
CaiI	CAGNNNCTG	2 cut(s)	687, 1016
CfoI	GCGC	1 cut(s)	623
Cfr13I	GGNCC	3 cut(s)	435, 800, 824
CseI	GACGC	2 cut(s)	664, 801
Csp6I	GTAC	5 cut(s)	220, 316, 753, 774, 808
CspCI	CAANNNNNGTGG	2 cut(s)	427, 462
CviAII	CATG	7 cut(s)	29, 443, 503, 771, 793, 1069, 1097
CviQI	GTAC	5 cut(s)	220, 316, 753, 774, 808
DdeI	CTNAG	1 cut(s)	585
DpnI	GATC	1 cut(s)	1015
DpnII	GATC	1 cut(s)	1013
EaeI	YGGCCR	4 cut(s)	102, 282, 300, 543
Eam1104I	CTCTTC	1 cut(s)	191
EarI	CTCTTC	1 cut(s)	191
Eco130I	CCWWGG	1 cut(s)	552
Eco31I	GGTCTC	1 cut(s)	1070
Eco47I	GGWCC	1 cut(s)	435
Eco57I	CTGAAG	1 cut(s)	133
Eco88I	CYCGRG	1 cut(s)	1041
EcoO109I	RGGNCCY	1 cut(s)	800
EcoRII	CCWGG	1 cut(s)	738
EcoT14I	CCWWGG	1 cut(s)	552
ErhI	CCWWGG	1 cut(s)	552
FaeI	CATG	7 cut(s)	32, 446, 506, 774, 796, 1072, 1100
FaqI	GGGAC	3 cut(s)	640, 698, 757
FatI	CATG	7 cut(s)	28, 442, 502, 770, 792, 1068, 1096
FblI	GTMKAC	1 cut(s)	678
Fnu4HI	GCNGC	6 cut(s)	105, 492, 495, 624, 881, 922
FokI	GGATG	1 cut(s)	263
Fsp4HI	GCNGC	6 cut(s)	105, 492, 495, 624, 881, 922
FspBI	CTAG	2 cut(s)	899, 1114
GlaI	GCGC	1 cut(s)	622
GluI	GCNGC	6 cut(s)	105, 492, 495, 624, 881, 922
GsuI	CTGGAG	2 cut(s)	99, 777
HaeIII	GGCC	6 cut(s)	104, 284, 302, 545, 802, 826
HapII	CCGG	1 cut(s)	281
HgaI	GACGC	2 cut(s)	664, 801
HhaI	GCGC	1 cut(s)	623
Hin1II	CATG	7 cut(s)	32, 446, 506, 774, 796, 1072, 1100
Hin6I	GCGC	1 cut(s)	621
HinP1I	GCGC	1 cut(s)	621
HincII	GTYRAC	6 cut(s)	85, 240, 272, 448, 679, 1094
HindII	GTYRAC	6 cut(s)	85, 240, 272, 448, 679, 1094
HinfI	GANTC	3 cut(s)	81, 970, 1004
HpaI	GTTAAC	1 cut(s)	240
HpaII	CCGG	1 cut(s)	281
HphI	GGTGA	2 cut(s)	605, 979
Hpy166II	GTNNAC	7 cut(s)	69, 85, 240, 272, 448, 679, 1094
Hpy188I	TCNGA	2 cut(s)	768, 890
Hpy188III	TCNNGA	3 cut(s)	127, 419, 1043
Hpy8I	GTNNAC	7 cut(s)	69, 85, 240, 272, 448, 679, 1094
Hpy99I	CGWCG	1 cut(s)	680
HpyAV	CCTTC	2 cut(s)	346, 958
HpyCH4III	ACNGT	5 cut(s)	163, 730, 748, 1081, 1103
HpyCH4IV	ACGT	2 cut(s)	242, 318
HpyCH4V	TGCA	7 cut(s)	28, 227, 248, 457, 786, 883, 1010
HpyF10VI	GCNNNNNNNGC	3 cut(s)	179, 233, 254
HpyF3I	CTNAG	1 cut(s)	585
HpySE526I	ACGT	2 cut(s)	242, 318
Hsp92II	CATG	7 cut(s)	32, 446, 506, 774, 796, 1072, 1100
HspAI	GCGC	1 cut(s)	621
KspAI	GTTAAC	1 cut(s)	240
Kzo9I	GATC	1 cut(s)	1013
LmnI	GCTCC	6 cut(s)	178, 200, 340, 389, 554, 902
Lsp1109I	GCAGC	4 cut(s)	503, 506, 635, 867
MaeI	CTAG	2 cut(s)	899, 1114
MaeII	ACGT	2 cut(s)	242, 318
MaeIII	GTNAC	3 cut(s)	207, 557, 1081
MalI	GATC	1 cut(s)	1015
MbiI	CCGCTC	1 cut(s)	905
MboI	GATC	1 cut(s)	1013
MboII	GAAGA	2 cut(s)	178, 524
MflI	RGATCY	1 cut(s)	1013
MlsI	TGGCCA	2 cut(s)	302, 545
MluCI	AATT	5 cut(s)	214, 332, 573, 630, 1026
MluI	ACGCGT	1 cut(s)	810
MluNI	TGGCCA	2 cut(s)	302, 545
MlyI	GAGTC	1 cut(s)	90
MmeI	TCCRAC	3 cut(s)	301, 689, 849
MnlI	CCTC	8 cut(s)	481, 680, 791, 860, 896, 991, 994, 1050
Mox20I	TGGCCA	2 cut(s)	302, 545
MscI	TGGCCA	2 cut(s)	302, 545
MseI	TTAA	5 cut(s)	18, 50, 239, 336, 374
MslI	CAYNNNNRTG	2 cut(s)	507, 791
Msp20I	TGGCCA	2 cut(s)	302, 545
MspI	CCGG	1 cut(s)	281
MspR9I	CCNGG	2 cut(s)	281, 740
MvaI	CCWGG	1 cut(s)	740
MvnI	CGCG	1 cut(s)	812
MwoI	GCNNNNNNNGC	3 cut(s)	179, 233, 254
NciI	CCSGG	1 cut(s)	281
NdeII	GATC	1 cut(s)	1013
NlaIII	CATG	7 cut(s)	32, 446, 506, 774, 796, 1072, 1100
NlaIV	GGNNCC	2 cut(s)	692, 981
NmuCI	GTSAC	2 cut(s)	207, 1081
NspI	RCATGY	1 cut(s)	774
PciI	ACATGT	1 cut(s)	770
PfeI	GAWTC	2 cut(s)	970, 1004
PkrI	GCNGC	6 cut(s)	106, 493, 496, 625, 882, 923
PleI	GAGTC	1 cut(s)	89
PpsI	GAGTC	1 cut(s)	89
PscI	ACATGT	1 cut(s)	770
PshBI	ATTAAT	1 cut(s)	374
Psp6I	CCWGG	1 cut(s)	738
PspGI	CCWGG	1 cut(s)	738
PspN4I	GGNNCC	2 cut(s)	692, 981
PspPI	GGNCC	3 cut(s)	435, 800, 824
PstI	CTGCAG	1 cut(s)	1012
PstNI	CAGNNNCTG	2 cut(s)	687, 1016
PsuI	RGATCY	1 cut(s)	1013
RsaI	GTAC	5 cut(s)	221, 317, 754, 775, 809
RsaNI	GTAC	5 cut(s)	220, 316, 753, 774, 808
RseI	CAYNNNNRTG	2 cut(s)	507, 791
SalI	GTCGAC	1 cut(s)	677
SaqAI	TTAA	5 cut(s)	18, 50, 239, 336, 374
SatI	GCNGC	6 cut(s)	105, 492, 495, 624, 881, 922
Sau3AI	GATC	1 cut(s)	1013
Sau96I	GGNCC	3 cut(s)	435, 800, 824
SchI	GAGTC	1 cut(s)	90
ScrFI	CCNGG	2 cut(s)	281, 740
SfcI	CTRYAG	2 cut(s)	1008, 1017
SinI	GGWCC	1 cut(s)	435
SmiMI	CAYNNNNRTG	2 cut(s)	507, 791
Sse9I	AATT	5 cut(s)	214, 332, 573, 630, 1026
SsiI	CCGC	3 cut(s)	105, 905, 921
SspI	AATATT	1 cut(s)	715
SspMI	CTAG	2 cut(s)	899, 1114
StyD4I	CCNGG	2 cut(s)	279, 738
StyI	CCWWGG	1 cut(s)	552
TaaI	ACNGT	5 cut(s)	163, 730, 748, 1081, 1103
TaiI	ACGT	2 cut(s)	245, 321
TaqI	TCGA	1 cut(s)	678
TasI	AATT	5 cut(s)	214, 332, 573, 630, 1026
TatI	WGTACW	2 cut(s)	219, 773
TauI	GCSGC	2 cut(s)	107, 924
TfiI	GAWTC	2 cut(s)	970, 1004
Tru1I	TTAA	5 cut(s)	18, 50, 239, 336, 374
Tru9I	TTAA	5 cut(s)	18, 50, 239, 336, 374
TseFI	GTSAC	2 cut(s)	207, 1081
TseI	GCWGC	4 cut(s)	491, 494, 623, 880
Tsp45I	GTSAC	2 cut(s)	207, 1081
TspDTI	ATGAA	1 cut(s)	1085
VpaK11BI	GGWCC	1 cut(s)	435
VspI	ATTAAT	1 cut(s)	374
XapI	RAATTY	2 cut(s)	214, 332
XceI	RCATGY	1 cut(s)	774
XmiI	GTMKAC	1 cut(s)	678
XspI	CTAG	2 cut(s)	899, 1114

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.