Rh1AG392800

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000337
Physical Location & Seq
Forward (+)
62139466 .. 62141510
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG392800.1

Sequence Viewer

Length: 1107 bp
ATGGTGCAGAATCATGAGGCTTCTTCTAATTTGAAGCCGGTGATTATGATGGTGGTTACCCAGATTTTGTATTCTGTGATGAACATAATGTACAAACTAGTTGCAGTAACAGGGATGAACTTGAATGTTTTCGTTGCCTACCGACTGATGTTTTCAGCGGCCTTTATGGTTCCACTGGCTCTGATACTTGAGAGGAAGAGAAGGCCAAAACTTACTTGGATTGTGTTGTTTCAAGCTTTTCTCTGCGGCCTAATTGGGGCTGCCGCGACACAAAACTTATATATTATGAGTCTGGCGTTAACATCACCCACATTTCTTGCAGCCATCTCCAATCTCCTCCCAGCCATTACCTTCGTGGTTGCTCTATGTTTTCGGCAGGAGAAGTTAACATTGGGATATTCCGGCATAGCCAAAATTGTTGGGACCGTGGTTGGGATAGGAGGAGCAATGCTTTTTACTTTCTACCGAGGACCTGTATTTACCATTTGGTCCACTCATATTGACCTTCTACGGCAATATCACAACACTAGTAGCGTCTCATCCTCAAGCCACAAGAGCAATCCTGCGTTGGGCTTCCTGGCAGGCTTCGGCAGCATCTTGTGCTTCGGAATATGGCTCACAGTTCAGGCGAAGATGAGTAAGAGATATCCCTGTCCTTACTCGAGCACGGCTTTGATGTGCATCATGGGATCCATCCAGTCCGTTATTTTTGCGTTTTGCGTGGAGAGAGATTGGAATCAATGGAAGATGGGTTGGGATATTAGGCTGTGGACTGCTGCATATTCGGGAATCGTCGTTTCTGGAGTGGCAGTGGTTCTGATGTCTTGGTGCGTACAAAAGCGAGGGGCACTTTTTGTGTCAATTTTCGCTCCACTGTTACTGTTGATGGTAGCCCTTGCTAGTTCCTTGTTGTTGGAGGAAAAATTTAGCCTCGGACGTGTATTAGGTGCAGTTCTAATTGTGTGCGGGCTATACGTGGTGCTGTGGGGAAAAAGCAAAGAAGAACCATCATTGGTTGTATTACCCTCATCGACAATAGCTCCCCGAGATTCCAAGTCCATAGATAGAGTTCCAGTTCCAGTTCCAGTTCTAGCGAGTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

368

Amino Acids

40.27

Weight (kDa)

9.67

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 13 - 153 1.5e-11 EamA-like transporter family
EamA PF00892 190 - 328 6.4e-15 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 266
AciI CCGC 4 cut(s) 158, 246, 264, 966
AclWI GGATC 2 cut(s) 684, 697
AcsI RAATTY 1 cut(s) 923
AfaI GTAC 2 cut(s) 92, 834
AfiI CCNNNNNNNGG 3 cut(s) 256, 432, 569
AflIII ACRYGT 1 cut(s) 937
AgsI TTSAA 3 cut(s) 34, 124, 233
AhlI ACTAGT 2 cut(s) 97, 527
AjiI CACGTC 1 cut(s) 938
AjnI CCWGG 1 cut(s) 576
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 2 cut(s) 236, 1040
AluI AGCT 2 cut(s) 236, 1040
Alw21I GWGCWC 1 cut(s) 668
Alw26I GTCTC 1 cut(s) 541
AlwI GGATC 2 cut(s) 684, 697
Ama87I CYCGRG 2 cut(s) 661, 1044
AoxI GGCC 3 cut(s) 159, 203, 247
ApeKI GCWGC 4 cut(s) 260, 320, 591, 776
ApoI RAATTY 1 cut(s) 923
Asp700I GAANNNNTTC 1 cut(s) 128
AspS9I GGNCC 3 cut(s) 423, 470, 489
AsuHPI GGTGA 2 cut(s) 52, 297
AvaI CYCGRG 2 cut(s) 661, 1044
AvaII GGWCC 3 cut(s) 423, 470, 489
BaeGI GKGCMC 1 cut(s) 850
BamHI GGATCC 1 cut(s) 689
Bbv12I GWGCWC 1 cut(s) 668
BbvI GCAGC 4 cut(s) 247, 332, 603, 763
BccI CCATC 6 cut(s) 43, 332, 701, 742, 880, 1015
BceAI ACGGC 2 cut(s) 527, 684
BciT130I CCWGG 1 cut(s) 578
BcoDI GTCTC 1 cut(s) 541
BcuI ACTAGT 2 cut(s) 97, 527
BfaI CTAG 4 cut(s) 98, 528, 900, 1091
BisI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
BlsI GCNGC 7 cut(s) 160, 248, 262, 265, 322, 593, 778
Bme1390I CCNGG 1 cut(s) 578
Bme18I GGWCC 3 cut(s) 423, 470, 489
BmeT110I CYCGRG 2 cut(s) 661, 1044
BmgBI CACGTC 1 cut(s) 938
BmgT120I GGNCC 3 cut(s) 423, 470, 489
BmiI GGNNCC 3 cut(s) 171, 424, 691
BmrFI CCNGG 1 cut(s) 578
BmsI GCATC 2 cut(s) 603, 690
BpmI CTGGAG 1 cut(s) 822
BpuEI CTTGAG 2 cut(s) 209, 529
BsaAI YACGTR 1 cut(s) 976
BsaBI GATNNNNATC 2 cut(s) 680, 735
BsaJI CCNNGG 3 cut(s) 426, 466, 931
BsaXI ACNNNNNCTCC 4 cut(s) 795, 825, 1024, 1054
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 432, 569
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 5 cut(s) 180, 697, 1073, 1079, 1085
Bse3DI GCAATG 1 cut(s) 453
Bse8I GATNNNNATC 2 cut(s) 680, 735
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 3 cut(s) 426, 466, 931
BseGI GGATG 3 cut(s) 120, 539, 693
BseJI GATNNNNATC 2 cut(s) 680, 735
BseLI CCNNNNNNNGG 3 cut(s) 256, 432, 569
BseMI GCAATG 1 cut(s) 453
BseNI ACTGG 5 cut(s) 180, 697, 1073, 1079, 1085
BseRI GAGGAG 2 cut(s) 326, 456
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 4 cut(s) 247, 332, 603, 763
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 2 cut(s) 26, 969
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 3 cut(s) 161, 205, 249
BsiHKAI GWGCWC 1 cut(s) 668
BsiHKCI CYCGRG 2 cut(s) 661, 1044
BsiSI CCGG 2 cut(s) 38, 402
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 3 cut(s) 256, 432, 569
BsmAI GTCTC 1 cut(s) 541
BsmBI CGTCTC 1 cut(s) 541
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 3 cut(s) 161, 205, 249
BsoBI CYCGRG 2 cut(s) 661, 1044
Bsp1286I GDGCHC 2 cut(s) 668, 850
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 1 cut(s) 689
BspACI CCGC 4 cut(s) 158, 246, 264, 966
BspANI GGCC 3 cut(s) 161, 205, 249
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 3 cut(s) 171, 424, 691
BspPI GGATC 2 cut(s) 684, 697
BsrDI GCAATG 1 cut(s) 453
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 5 cut(s) 180, 697, 1073, 1079, 1085
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 3 cut(s) 426, 466, 931
BssMI GATC 1 cut(s) 689
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 4 cut(s) 427, 622, 876, 882
Bst6I CTCTTC 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 600
BstAUI TGTACA 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 976
BstC8I GCNNGC 2 cut(s) 583, 968
BstDSI CCRYGG 1 cut(s) 426
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 3 cut(s) 120, 539, 693
BstFNI CGCG 1 cut(s) 266
BstKTI GATC 1 cut(s) 692
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 1 cut(s) 689
BstMWI GCNNNNNNNGC 3 cut(s) 555, 591, 600
BstNI CCWGG 1 cut(s) 578
BstPI GGTNACC 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 576
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 4 cut(s) 247, 332, 603, 763
BstX2I RGATCY 1 cut(s) 689
BstYI RGATCY 1 cut(s) 689
BsuRI GGCC 3 cut(s) 161, 205, 249
BtgI CCRYGG 1 cut(s) 426
BtrI CACGTC 1 cut(s) 938
BtsCI GGATG 3 cut(s) 120, 539, 693
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 3 cut(s) 173, 816, 872
Cac8I GCNNGC 2 cut(s) 583, 968
CciI TCATGA 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 3 cut(s) 423, 470, 489
CseI GACGC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 91, 833
CspCI CAANNNNNGTGG 4 cut(s) 298, 333, 481, 516
CviAII CATG 2 cut(s) 14, 685
CviQI GTAC 2 cut(s) 91, 833
DpnI GATC 1 cut(s) 691
DpnII GATC 1 cut(s) 689
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 647
Eco47I GGWCC 3 cut(s) 423, 470, 489
Eco88I CYCGRG 2 cut(s) 661, 1044
Eco91I GGTNACC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 470
EcoO65I GGTNACC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 576
EcoRV GATATC 1 cut(s) 647
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 2 cut(s) 17, 688
FaqI GGGAC 1 cut(s) 436
FatI CATG 2 cut(s) 13, 684
FauI CCCGC 1 cut(s) 959
Fnu4HI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
FokI GGATG 3 cut(s) 127, 526, 680
Fsp4HI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
FspBI CTAG 4 cut(s) 98, 528, 900, 1091
GluI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
GsaI CCCAGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 822
HaeIII GGCC 3 cut(s) 161, 205, 249
HapII CCGG 2 cut(s) 38, 402
HgaI GACGC 1 cut(s) 523
Hin1II CATG 2 cut(s) 17, 688
HincII GTYRAC 2 cut(s) 300, 387
HindII GTYRAC 2 cut(s) 300, 387
HindIII AAGCTT 1 cut(s) 234
HinfI GANTC 5 cut(s) 10, 289, 736, 789, 1049
HpaI GTTAAC 2 cut(s) 300, 387
HpaII CCGG 2 cut(s) 38, 402
HphI GGTGA 2 cut(s) 52, 297
Hpy166II GTNNAC 4 cut(s) 300, 387, 492, 771
Hpy188I TCNGA 4 cut(s) 183, 608, 819, 935
Hpy188III TCNNGA 3 cut(s) 14, 786, 801
Hpy8I GTNNAC 4 cut(s) 300, 387, 492, 771
Hpy99I CGWCG 1 cut(s) 797
HpyAV CCTTC 3 cut(s) 195, 361, 515
HpyCH4III ACNGT 4 cut(s) 427, 622, 876, 882
HpyCH4IV ACGT 2 cut(s) 937, 975
HpyCH4V TGCA 6 cut(s) 7, 104, 320, 681, 779, 950
HpyF10VI GCNNNNNNNGC 3 cut(s) 555, 591, 600
HpySE526I ACGT 2 cut(s) 937, 975
Hsp92II CATG 2 cut(s) 17, 688
KspAI GTTAAC 2 cut(s) 300, 387
Kzo9I GATC 1 cut(s) 689
LmnI GCTCC 3 cut(s) 443, 874, 1045
Lsp1109I GCAGC 4 cut(s) 247, 332, 603, 763
LweI GCATC 2 cut(s) 603, 690
MaeI CTAG 4 cut(s) 98, 528, 900, 1091
MaeII ACGT 2 cut(s) 937, 975
MaeIII GTNAC 3 cut(s) 55, 106, 876
MalI GATC 1 cut(s) 691
MboI GATC 1 cut(s) 689
MboII GAAGA 5 cut(s) 15, 208, 643, 757, 1013
MflI RGATCY 1 cut(s) 689
MhlI GDGCHC 2 cut(s) 668, 850
MluCI AATT 6 cut(s) 28, 252, 414, 861, 923, 957
MlyI GAGTC 1 cut(s) 298
MmeI TCCRAC 2 cut(s) 894, 1081
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 2 cut(s) 299, 386
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 2 cut(s) 38, 402
MspR9I CCNGG 1 cut(s) 578
MvaI CCWGG 1 cut(s) 578
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 3 cut(s) 555, 591, 600
NdeII GATC 1 cut(s) 689
NlaIII CATG 2 cut(s) 17, 688
NlaIV GGNNCC 3 cut(s) 171, 424, 691
PaeR7I CTCGAG 1 cut(s) 661
PagI TCATGA 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 4 cut(s) 10, 736, 789, 1049
PkrI GCNGC 7 cut(s) 160, 248, 262, 265, 322, 593, 778
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Ppu21I YACGTR 1 cut(s) 976
PpuMI RGGWCCY 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 576
PspEI GGTNACC 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 3 cut(s) 171, 424, 691
PspPI GGNCC 3 cut(s) 423, 470, 489
PspPPI RGGWCCY 1 cut(s) 470
PspXI VCTCGAGB 1 cut(s) 661
PsrI GAACNNNNNNTAC 2 cut(s) 74, 106
PsuI RGATCY 1 cut(s) 689
RsaI GTAC 2 cut(s) 92, 834
RsaNI GTAC 2 cut(s) 91, 833
SaqAI TTAA 2 cut(s) 299, 386
SatI GCNGC 7 cut(s) 159, 247, 261, 264, 321, 592, 777
Sau3AI GATC 1 cut(s) 689
Sau96I GGNCC 3 cut(s) 423, 470, 489
SchI GAGTC 1 cut(s) 298
ScrFI CCNGG 1 cut(s) 578
SduI GDGCHC 2 cut(s) 668, 850
SetI ASST 8 cut(s) 238, 353, 475, 507, 940, 949, 978, 1042
SfaNI GCATC 2 cut(s) 603, 690
Sfr274I CTCGAG 1 cut(s) 661
SinI GGWCC 3 cut(s) 423, 470, 489
SlaI CTCGAG 1 cut(s) 661
SmlI CTYRAG 3 cut(s) 188, 544, 661
SmoI CTYRAG 3 cut(s) 188, 544, 661
SpeI ACTAGT 2 cut(s) 97, 527
Sse9I AATT 6 cut(s) 28, 252, 414, 861, 923, 957
SsiI CCGC 4 cut(s) 158, 246, 264, 966
SspMI CTAG 4 cut(s) 98, 528, 900, 1091
StyD4I CCNGG 1 cut(s) 576
TaaI ACNGT 4 cut(s) 427, 622, 876, 882
TaiI ACGT 2 cut(s) 940, 978
TaqI TCGA 2 cut(s) 662, 1031
TasI AATT 6 cut(s) 28, 252, 414, 861, 923, 957
TatI WGTACW 1 cut(s) 90
TauI GCSGC 3 cut(s) 161, 249, 266
TfiI GAWTC 4 cut(s) 10, 736, 789, 1049
Tru1I TTAA 2 cut(s) 299, 386
Tru9I TTAA 2 cut(s) 299, 386
TscAI CASTG 3 cut(s) 180, 816, 879
TseI GCWGC 4 cut(s) 260, 320, 591, 776
TspDTI ATGAA 2 cut(s) 95, 131
TspGWI ACGGA 1 cut(s) 691
TspRI CASTG 3 cut(s) 180, 816, 879
VpaK11BI GGWCC 3 cut(s) 423, 470, 489
XapI RAATTY 1 cut(s) 923
XcmI CCANNNNNNNNNTGG 2 cut(s) 213, 352
XhoI CTCGAG 1 cut(s) 661
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 4 cut(s) 98, 528, 900, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.