Rroxscaffold_3G00234590

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000337
Physical Location & Seq
Forward (+)
21094781 .. 21097820
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00234590.1

Sequence Viewer

Length: 612 bp
ATGCAGAATTGTAAGGCAATGGAGGGGATGAAGCCTGTTATGGTTATGGTGTTGGTTCAGAGTCTATTTGCAGGGATGAATGTATTGTACAAATTAGTGGCAAATGATGGCATAAATTTCAGGATTCTCATTGCCTACCGGATGGTGTTTGCTGCTGCCTTCATGACTCCTCTTGCTCTCGTTTTCGAAATAAGGGATTGGAAGAAGTGGAAGTTGGGATGGAACATTCAACTTCTTGCAATGTCTTATTCGGGTATTTTCAACTCTGGAATAGCCGTGACTCTAATGGCATGGTGCGTAAAGAAGGGAGGACCAGTGTTCGTTGCAAGTTTTCAGCCTCTCTTGCTTGTGATGGTTGCAGTTGCTGGGTCTCTGCTTCTGGATGAGAAGTTGCACCTTGGAAGTGTATTAGGTGGATTGCTGATTGTGAGTGGACTATACATGGTATTGTGGGGTAAGAGTAAGGAAATGAAGAATCAGATAACTCCATTACCGGCGCCAGCAAGCCTCCAATGTCAAGATGAACACCAATCTCCTACGATCGAAGTCATTACATCATCTTCTATGGAGGACATGAACCACATCAATGATAGAAATGTGACAAATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

203

Amino Acids

22.44

Weight (kDa)

8.78

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 14 - 150 2.8e-15 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 496
AcsI RAATTY 1 cut(s) 115
AcyI GRCGYC 1 cut(s) 497
AfaI GTAC 1 cut(s) 89
AgsI TTSAA 2 cut(s) 230, 262
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
Alw26I GTCTC 1 cut(s) 375
AlwNI CAGNNNCTG 1 cut(s) 365
ApeKI GCWGC 2 cut(s) 152, 155
ApoI RAATTY 1 cut(s) 115
AspLEI GCGC 1 cut(s) 499
AspS9I GGNCC 1 cut(s) 311
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 1 cut(s) 311
BanI GGYRCC 1 cut(s) 496
BbvI GCAGC 2 cut(s) 139, 142
BccI CCATC 4 cut(s) 101, 136, 213, 346
BceAI ACGGC 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 375
BfoI RGCGCY 1 cut(s) 500
BisI GCNGC 2 cut(s) 153, 156
BlsI GCNGC 2 cut(s) 154, 157
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 498
Bpu14I TTCGAA 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 497
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 397
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 1 cut(s) 314
Bse3DI GCAATG 3 cut(s) 24, 129, 246
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 5 cut(s) 33, 81, 147, 224, 388
BseMI GCAATG 3 cut(s) 24, 129, 246
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 2 cut(s) 139, 142
BseYI CCCAGC 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 543
BshNI GGYRCC 1 cut(s) 496
BsiEI CGRYCG 1 cut(s) 543
BsiSI CCGG 2 cut(s) 139, 494
BsmAI GTCTC 1 cut(s) 375
Bso31I GGTCTC 1 cut(s) 375
Bsp119I TTCGAA 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 1 cut(s) 540
BspHI TCATGA 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 498
BspT104I TTCGAA 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 496
BspTNI GGTCTC 1 cut(s) 375
BsrDI GCAATG 3 cut(s) 24, 129, 246
BsrFI RCCGGY 1 cut(s) 493
BsrGI TGTACA 1 cut(s) 87
BsrI ACTGG 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 1 cut(s) 540
BssNI GRCGYC 1 cut(s) 497
BssT1I CCWWGG 1 cut(s) 397
BstACI GRCGYC 1 cut(s) 497
BstAUI TGTACA 1 cut(s) 87
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 501, 505
BstF5I GGATG 5 cut(s) 33, 81, 147, 224, 388
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 1 cut(s) 499
BstKTI GATC 1 cut(s) 543
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 1 cut(s) 540
BstMCI CGRYCG 1 cut(s) 543
BstMWI GCNNNNNNNGC 1 cut(s) 343
BstV1I GCAGC 2 cut(s) 139, 142
BtsCI GGATG 5 cut(s) 33, 81, 147, 224, 388
BtsIMutI CAGTG 1 cut(s) 321
Cac8I GCNNGC 2 cut(s) 501, 505
CaiI CAGNNNCTG 1 cut(s) 365
CciI TCATGA 1 cut(s) 162
CfoI GCGC 1 cut(s) 499
Cfr10I RCCGGY 1 cut(s) 493
Cfr13I GGNCC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 88
CviAII CATG 4 cut(s) 163, 291, 442, 574
CviJI RGCY 4 cut(s) 34, 275, 337, 507
CviKI_1 RGCY 4 cut(s) 34, 275, 337, 507
CviQI GTAC 1 cut(s) 88
DinI GGCGCC 1 cut(s) 498
DpnI GATC 1 cut(s) 542
DpnII GATC 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 397
Eco31I GGTCTC 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 311
EcoT14I CCWWGG 1 cut(s) 397
EgeI GGCGCC 1 cut(s) 498
EheI GGCGCC 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 4 cut(s) 166, 294, 445, 577
FaiI YATR 9 cut(s) 41, 47, 113, 164, 292, 439, 443, 566, 575
FatI CATG 4 cut(s) 162, 290, 441, 573
Fnu4HI GCNGC 2 cut(s) 153, 156
FokI GGATG 5 cut(s) 40, 88, 154, 231, 395
Fsp4HI GCNGC 2 cut(s) 153, 156
GlaI GCGC 1 cut(s) 498
GluI GCNGC 2 cut(s) 153, 156
GsaI CCCAGC 1 cut(s) 369
HaeII RGCGCY 1 cut(s) 500
HapII CCGG 2 cut(s) 139, 494
HhaI GCGC 1 cut(s) 499
Hin1I GRCGYC 1 cut(s) 497
Hin1II CATG 4 cut(s) 166, 294, 445, 577
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HinfI GANTC 5 cut(s) 61, 124, 166, 280, 475
HpaII CCGG 2 cut(s) 139, 494
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 2 cut(s) 60, 480
Hpy188III TCNNGA 5 cut(s) 121, 163, 267, 380, 518
Hpy8I GTNNAC 1 cut(s) 434
HpyAV CCTTC 2 cut(s) 169, 298
HpyCH4V TGCA 6 cut(s) 4, 71, 239, 326, 359, 394
HpyF10VI GCNNNNNNNGC 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 497
Hsp92II CATG 4 cut(s) 166, 294, 445, 577
HspAI GCGC 1 cut(s) 497
KasI GGCGCC 1 cut(s) 496
Kzo9I GATC 1 cut(s) 540
Lsp1109I GCAGC 2 cut(s) 139, 142
MaeIII GTNAC 2 cut(s) 277, 598
MalI GATC 1 cut(s) 542
MboI GATC 1 cut(s) 540
MboII GAAGA 3 cut(s) 214, 484, 552
MluCI AATT 4 cut(s) 7, 92, 115, 607
Mly113I GGCGCC 1 cut(s) 497
MlyI GAGTC 3 cut(s) 70, 160, 274
MnlI CCTC 6 cut(s) 16, 180, 302, 348, 518, 562
MslI CAYNNNNRTG 1 cut(s) 585
MspI CCGG 2 cut(s) 139, 494
MwoI GCNNNNNNNGC 1 cut(s) 343
NarI GGCGCC 1 cut(s) 497
NdeII GATC 1 cut(s) 540
NlaIII CATG 4 cut(s) 166, 294, 445, 577
NlaIV GGNNCC 1 cut(s) 498
NmuCI GTSAC 2 cut(s) 277, 598
NspV TTCGAA 1 cut(s) 186
PagI TCATGA 1 cut(s) 162
PfeI GAWTC 2 cut(s) 124, 475
PkrI GCNGC 2 cut(s) 154, 157
Ple19I CGATCG 1 cut(s) 543
PleI GAGTC 3 cut(s) 69, 160, 274
PluTI GGCGCC 1 cut(s) 500
PpsI GAGTC 3 cut(s) 69, 160, 274
PspFI CCCAGC 1 cut(s) 365
PspN4I GGNNCC 1 cut(s) 498
PspPI GGNCC 1 cut(s) 311
PstNI CAGNNNCTG 1 cut(s) 365
PvuI CGATCG 1 cut(s) 543
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
RseI CAYNNNNRTG 1 cut(s) 585
SatI GCNGC 2 cut(s) 153, 156
Sau3AI GATC 1 cut(s) 540
Sau96I GGNCC 1 cut(s) 311
SchI GAGTC 3 cut(s) 70, 160, 274
SetI ASST 2 cut(s) 399, 415
SfoI GGCGCC 1 cut(s) 498
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 585
Sse9I AATT 4 cut(s) 7, 92, 115, 607
SspDI GGCGCC 1 cut(s) 496
StyI CCWWGG 1 cut(s) 397
TaqI TCGA 2 cut(s) 186, 543
TasI AATT 4 cut(s) 7, 92, 115, 607
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 2 cut(s) 124, 475
TscAI CASTG 1 cut(s) 321
TseFI GTSAC 2 cut(s) 277, 598
TseI GCWGC 2 cut(s) 152, 155
Tsp45I GTSAC 2 cut(s) 277, 598
TspDTI ATGAA 6 cut(s) 44, 92, 151, 485, 537, 590
TspRI CASTG 1 cut(s) 321
VpaK11BI GGWCC 1 cut(s) 311
XapI RAATTY 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.