Rh7CG398300

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000337
Physical Location & Seq
Forward (+)
50820926 .. 50828723
7798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG398300.1

Sequence Viewer

Length: 996 bp
ATGGGGTCTCTCTCTAAACACTGCATAGAGCTGAGGAATCGGAGGGGCTGCGAGTGTATCTGTAATTTGTTAAATGGGTTGAAACCAGTGCTGGGAATGCTGTTGGTAAGTGTTTTAGGTGCAGTGTTGAACATATTCTACAAACTGGTCGCTATTGATGGAATGAACTTGAGGGTCTTCGTCGCCTACCGCGTCATCTTGGCCACTGTCTTCTTGGTGCCTCTCGCTCACGTAGTTGAAAGGAACAAAAGACCAAAACTTACGTGGATTATACTACTGCAAGCATTTCTCTGTGGGTTATTTGGGGTAATACTGGCTCTAAACCTATACCTGGAGAGCATAGTATTGGTATCTGCTACATTCGCTGCCGCGATGAATAATCTGTTACCCGCCATCACCTTCATAATGGCGGTATGTTTGAGGATGGAGAAATTAGTCATAAGAAGCGTTCCAGGGGCGGCGAAAGTTGTCGGCTCACTGGTCGGGATCGGAGGGGCGATGATCTTCACTCTCTACAAAGGCACAATTATACAAATCTGGTCTACACATCCTCACCTTTTTCATATGAAGAAAAACACTTCTTCATCACAGGCAAATCACTCTGCCAATCCAGCCTTGGGCTCTGTTTTAGCTGTAGTCAGCTGTTTATGCGCTGCAACTTGGTACATAATTCAGGGTAAAATGACTAAAACATACCCATGCGTATTGTCCTGCACAGCTCTAGTCTCTGTAATGGCAACAATCCAATCTGTCATTTTGGCCTTATGTATGGAAAGGGACTGGAGTAAATGGAAGTTGGGTTGGAATATTAGACTTCTTGGAGTTGTTTATTCGGGAATAGTGAGTTCAGGACTTGCAATGTGCTTGACTTTATGGTGTATGAATATGAGAGGGCCGTTGTTTGTATCAAGTTTTAACCCTGTAACGCTCATAGTCGTAGCCATTTTCGGCTCTTTGCTGCTCCAAGAGAAGTTGTACCTAGGAAGGTATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

331

Amino Acids

36.21

Weight (kDa)

9.64

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 30 - 163 3.9e-13 EamA-like transporter family
EamA PF00892 206 - 327 1.1e-09 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccI GTMKAC 1 cut(s) 542
AccII CGCG 2 cut(s) 192, 371
AciI CCGC 5 cut(s) 190, 369, 390, 410, 458
AclWI GGATC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 201
AfaI GTAC 2 cut(s) 665, 977
AfiI CCNNNNNNNGG 3 cut(s) 92, 331, 617
AgsI TTSAA 3 cut(s) 82, 130, 239
AjnI CCWGG 2 cut(s) 330, 451
AluBI AGCT 4 cut(s) 31, 632, 642, 719
AluI AGCT 4 cut(s) 31, 632, 642, 719
Alw26I GTCTC 2 cut(s) 12, 730
AlwI GGATC 1 cut(s) 494
AoxI GGCC 3 cut(s) 201, 759, 893
ApeKI GCWGC 4 cut(s) 48, 365, 653, 958
Asp700I GAANNNNTTC 1 cut(s) 134
AspA2I CCTAGG 1 cut(s) 979
AspLEI GCGC 1 cut(s) 653
AspS9I GGNCC 1 cut(s) 893
AsuHPI GGTGA 2 cut(s) 388, 545
AvrII CCTAGG 1 cut(s) 979
BalI TGGCCA 1 cut(s) 203
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 623
BbsI GAAGAC 2 cut(s) 169, 202
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 4 cut(s) 35, 352, 640, 945
BccI CCATC 3 cut(s) 152, 401, 418
BceAI ACGGC 1 cut(s) 880
BciT130I CCWGG 2 cut(s) 332, 453
BcoDI GTCTC 2 cut(s) 12, 730
BfaI CTAG 2 cut(s) 722, 980
BfmI CTRYAG 1 cut(s) 633
BisI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
BlnI CCTAGG 1 cut(s) 979
BlsI GCNGC 6 cut(s) 50, 367, 370, 460, 655, 960
Bme1390I CCNGG 2 cut(s) 332, 453
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 2 cut(s) 332, 453
BpiI GAAGAC 2 cut(s) 169, 202
BpmI CTGGAG 2 cut(s) 353, 802
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 190
BsaAI YACGTR 2 cut(s) 232, 264
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 452, 615, 979
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 331, 617
Bse1I ACTGG 5 cut(s) 86, 150, 318, 483, 785
Bse3DI GCAATG 1 cut(s) 864
BseBI CCWGG 2 cut(s) 332, 453
BseDI CCNNGG 3 cut(s) 452, 615, 979
BseGI GGATG 2 cut(s) 429, 547
BseLI CCNNNNNNNGG 3 cut(s) 92, 331, 617
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 5 cut(s) 86, 150, 318, 483, 785
BseXI GCAGC 4 cut(s) 35, 352, 640, 945
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 2 cut(s) 141, 697
Bsh1236I CGCG 2 cut(s) 192, 371
BshFI GGCC 3 cut(s) 203, 761, 895
BshNI GGYRCC 1 cut(s) 217
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 3 cut(s) 92, 331, 617
BsmAI GTCTC 2 cut(s) 12, 730
BsmFI GGGAC 1 cut(s) 791
BsmI GAATGC 1 cut(s) 102
BsnI GGCC 3 cut(s) 203, 761, 895
Bso31I GGTCTC 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 623
Bsp143I GATC 2 cut(s) 486, 501
BspACI CCGC 5 cut(s) 190, 369, 390, 410, 458
BspANI GGCC 3 cut(s) 203, 761, 895
BspCNI CTCAG 1 cut(s) 24
BspFNI CGCG 2 cut(s) 192, 371
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 5 cut(s) 86, 150, 318, 483, 785
BssECI CCNNGG 3 cut(s) 452, 615, 979
BssMI GATC 2 cut(s) 486, 501
BssT1I CCWWGG 2 cut(s) 615, 979
Bst2UI CCWGG 2 cut(s) 332, 453
Bst4CI ACNGT 1 cut(s) 208
BstBAI YACGTR 2 cut(s) 232, 264
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 2 cut(s) 429, 547
BstFNI CGCG 2 cut(s) 192, 371
BstHHI GCGC 1 cut(s) 653
BstKTI GATC 2 cut(s) 489, 504
BstMAI GTCTC 2 cut(s) 12, 730
BstMBI GATC 2 cut(s) 486, 501
BstMWI GCNNNNNNNGC 4 cut(s) 97, 362, 611, 648
BstNI CCWGG 2 cut(s) 332, 453
BstSCI CCNGG 2 cut(s) 330, 451
BstSFI CTRYAG 1 cut(s) 633
BstUI CGCG 2 cut(s) 192, 371
BstV1I GCAGC 4 cut(s) 35, 352, 640, 945
BstV2I GAAGAC 2 cut(s) 169, 202
BsuRI GGCC 3 cut(s) 203, 761, 895
BtgZI GCGATG 2 cut(s) 386, 512
BtsCI GGATG 2 cut(s) 429, 547
BtsI GCAGTG 2 cut(s) 19, 129
BtsIMutI CAGTG 5 cut(s) 19, 93, 129, 204, 476
Cac8I GCNNGC 1 cut(s) 282
CfoI GCGC 1 cut(s) 653
Cfr13I GGNCC 1 cut(s) 893
CseI GACGC 1 cut(s) 181
Csp6I GTAC 2 cut(s) 664, 976
CviAII CATG 1 cut(s) 699
CviQI GTAC 2 cut(s) 664, 976
DdeI CTNAG 1 cut(s) 32
DpnI GATC 2 cut(s) 488, 503
DpnII GATC 2 cut(s) 486, 501
EaeI YGGCCR 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 615, 979
Eco24I GRGCYC 1 cut(s) 623
Eco31I GGTCTC 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 330, 451
EcoT14I CCWWGG 2 cut(s) 615, 979
EcoT38I GRGCYC 1 cut(s) 623
ErhI CCWWGG 2 cut(s) 615, 979
FaeI CATG 1 cut(s) 702
FaqI GGGAC 1 cut(s) 791
FatI CATG 1 cut(s) 698
FauI CCCGC 1 cut(s) 397
FauNDI CATATG 1 cut(s) 564
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
FokI GGATG 2 cut(s) 436, 534
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
FspBI CTAG 2 cut(s) 722, 980
GlaI GCGC 1 cut(s) 652
GluI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 2 cut(s) 353, 802
HaeIII GGCC 3 cut(s) 203, 761, 895
HgaI GACGC 1 cut(s) 181
HhaI GCGC 1 cut(s) 653
Hin1II CATG 1 cut(s) 702
Hin6I GCGC 1 cut(s) 651
HinP1I GCGC 1 cut(s) 651
HinfI GANTC 1 cut(s) 37
HphI GGTGA 2 cut(s) 388, 545
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 2 cut(s) 42, 491
Hpy188III TCNNGA 3 cut(s) 484, 834, 849
Hpy8I GTNNAC 1 cut(s) 543
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 409, 978
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 2 cut(s) 231, 263
HpyCH4V TGCA 6 cut(s) 24, 122, 280, 656, 714, 857
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 362, 611, 648
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 2 cut(s) 231, 263
Hsp92II CATG 1 cut(s) 702
HspAI GCGC 1 cut(s) 651
Kzo9I GATC 2 cut(s) 486, 501
LmnI GCTCC 1 cut(s) 966
Lsp1109I GCAGC 4 cut(s) 35, 352, 640, 945
MaeI CTAG 2 cut(s) 722, 980
MaeII ACGT 2 cut(s) 231, 263
MaeIII GTNAC 2 cut(s) 384, 922
MalI GATC 2 cut(s) 488, 503
MboI GATC 2 cut(s) 486, 501
MboII GAAGA 5 cut(s) 169, 202, 496, 573, 580
MhlI GDGCHC 1 cut(s) 623
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 4 cut(s) 64, 431, 525, 669
MluNI TGGCCA 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 782
MnlI CCTC 8 cut(s) 27, 36, 165, 231, 414, 485, 561, 884
Mox20I TGGCCA 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 134
MscI TGGCCA 1 cut(s) 203
MseI TTAA 2 cut(s) 71, 915
MslI CAYNNNNRTG 1 cut(s) 697
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 642
MspR9I CCNGG 2 cut(s) 332, 453
Mva1269I GAATGC 1 cut(s) 102
MvaI CCWGG 2 cut(s) 332, 453
MvnI CGCG 2 cut(s) 192, 371
MwoI GCNNNNNNNGC 4 cut(s) 97, 362, 611, 648
NdeI CATATG 1 cut(s) 564
NdeII GATC 2 cut(s) 486, 501
NlaIII CATG 1 cut(s) 702
NlaIV GGNNCC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 37
PkrI GCNGC 6 cut(s) 50, 367, 370, 460, 655, 960
Ppu21I YACGTR 2 cut(s) 232, 264
Psp6I CCWGG 2 cut(s) 330, 451
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 2 cut(s) 330, 451
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 893
PsrI GAACNNNNNNTAC 2 cut(s) 122, 154
PvuII CAGCTG 1 cut(s) 642
RsaI GTAC 2 cut(s) 665, 977
RsaNI GTAC 2 cut(s) 664, 976
RseI CAYNNNNRTG 1 cut(s) 697
SaqAI TTAA 2 cut(s) 71, 915
SatI GCNGC 6 cut(s) 49, 366, 369, 459, 654, 959
Sau3AI GATC 2 cut(s) 486, 501
Sau96I GGNCC 1 cut(s) 893
ScrFI CCNGG 2 cut(s) 332, 453
SduI GDGCHC 1 cut(s) 623
SfcI CTRYAG 1 cut(s) 633
SmiMI CAYNNNNRTG 1 cut(s) 697
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 4 cut(s) 64, 431, 525, 669
SsiI CCGC 5 cut(s) 190, 369, 390, 410, 458
SspI AATATT 1 cut(s) 808
SspMI CTAG 2 cut(s) 722, 980
StyD4I CCNGG 2 cut(s) 330, 451
StyI CCWWGG 2 cut(s) 615, 979
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 2 cut(s) 234, 266
TasI AATT 4 cut(s) 64, 431, 525, 669
TauI GCSGC 2 cut(s) 371, 461
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 2 cut(s) 71, 915
Tru9I TTAA 2 cut(s) 71, 915
TscAI CASTG 5 cut(s) 26, 93, 129, 211, 483
TseI GCWGC 4 cut(s) 48, 365, 653, 958
TspDTI ATGAA 7 cut(s) 179, 389, 391, 551, 573, 581, 896
TspRI CASTG 5 cut(s) 26, 93, 129, 211, 483
XcmI CCANNNNNNNNNTGG 3 cut(s) 211, 261, 613
XmaJI CCTAGG 1 cut(s) 979
XmiI GTMKAC 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 2 cut(s) 722, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.