Rh1DG387400

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000337
Physical Location & Seq
Forward (+)
60673079 .. 60695465
22387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG387400.1

Sequence Viewer

Length: 966 bp
ATGGTGCAAAATCATGAGGCTTCTTCTAATTTGAAGCCGGTGATTATGATGGTGGTTACCCAGATTTTGTATACTGTGATGAACATAATGTACAAACTAGTTGCAGTAACAGGGATGAACTTGAATGTTTTCGTTGCCTACCGATTGATGTTTTCAGCGGCCTTTATGATTCCACTGGCTCTGATACTTGAGAGGAAGAGAAGGCCAAAACTTACTTGGGTTGTATTGTTTCAAGCTTTTCTCTGCGGCCTAATTGGGGCTGCCGCGACACAAAACTTATATATTGTGAGTCTAGCGCTGACGTCACCCACATTTCTTGCAGCCATCTCCAATCTCCTCCCAGCCATTACCTTCGTGGTTGCTCTATGTTTTCGGCAGGAGAAGTTGACCTTGGGATATTCCGGCATAGCCAAAATTGTTGGGACCGTGGTTGGGATAGGAGGAGCTATGCCTTTTACTTTCTATCGTGGACCTGTATTTACCATTTGGTCCACTCATATTGACCTTCTACGGCAATATCACAACACTAGTAGCGTCTCATCCTCAGGCCACAAGAACAATCCTGCATTGGGCTTCCTGGCAGGCTTCGGCAGCATCTTATGCTTCGGAATATGGCTCACAGTTCAGGCGAAGATGAGTAAGAGATATCCCTGTCCTTACTCGAGCACGGCTTTAATGTGCATCATGGGATCCATTCAATCCGTTATTTTTGCGCTTTGCATGGAGAGAGATTGGAGTCAATGGAAGATGGGTTGGGATATTAGGTTGTGGACTGCTGCATACTCGGCAAATATGAGTAAGATATATCCGTGTCCTTACTCAAGCATGGCGTTGATGTCTTTCACGGGATCTACTCAGTCCGTTATTTTTGCGATTTGTGTGGAGAGAGATTGGAATCAATGGAAACTTGGTTGGGATATTACTGTGGACAGCTGCCTATTCGGTAACAATTACTCTTCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

321

Amino Acids

35.73

Weight (kDa)

9.36

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 13 - 149 7e-11 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 71
AccII CGCG 1 cut(s) 266
AciI CCGC 3 cut(s) 158, 246, 264
AclWI GGATC 3 cut(s) 684, 697, 856
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 1 cut(s) 92
AfeI AGCGCT 1 cut(s) 297
AfiI CCNNNNNNNGG 3 cut(s) 256, 432, 569
AgsI TTSAA 4 cut(s) 34, 124, 233, 698
AhlI ACTAGT 2 cut(s) 97, 527
AjnI CCWGG 1 cut(s) 576
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 4 cut(s) 236, 446, 933, 963
AluI AGCT 4 cut(s) 236, 446, 933, 963
Alw21I GWGCWC 1 cut(s) 668
Alw26I GTCTC 1 cut(s) 541
AlwI GGATC 3 cut(s) 684, 697, 856
Ama87I CYCGRG 1 cut(s) 661
Aor51HI AGCGCT 1 cut(s) 297
AoxI GGCC 4 cut(s) 159, 203, 247, 547
ApeKI GCWGC 5 cut(s) 260, 320, 591, 776, 933
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 2 cut(s) 298, 715
AspS9I GGNCC 3 cut(s) 423, 470, 489
AsuHPI GGTGA 2 cut(s) 52, 297
AvaI CYCGRG 1 cut(s) 661
AvaII GGWCC 3 cut(s) 423, 470, 489
AxyI CCTNAGG 1 cut(s) 544
BamHI GGATCC 1 cut(s) 689
Bbv12I GWGCWC 1 cut(s) 668
BbvI GCAGC 5 cut(s) 247, 332, 603, 763, 920
BccI CCATC 3 cut(s) 43, 332, 742
BceAI ACGGC 2 cut(s) 527, 684
BciT130I CCWGG 1 cut(s) 578
BcoDI GTCTC 1 cut(s) 541
BcuI ACTAGT 2 cut(s) 97, 527
BfaI CTAG 3 cut(s) 98, 293, 528
BfoI RGCGCY 1 cut(s) 299
BisI GCNGC 8 cut(s) 159, 247, 261, 264, 321, 592, 777, 934
BlsI GCNGC 8 cut(s) 160, 248, 262, 265, 322, 593, 778, 935
Bme1390I CCNGG 1 cut(s) 578
Bme18I GGWCC 3 cut(s) 423, 470, 489
BmeT110I CYCGRG 1 cut(s) 661
BmgT120I GGNCC 3 cut(s) 423, 470, 489
BmiI GGNNCC 2 cut(s) 424, 691
BmrFI CCNGG 1 cut(s) 578
BmsI GCATC 2 cut(s) 603, 690
BpuEI CTTGAG 2 cut(s) 209, 805
BsaBI GATNNNNATC 1 cut(s) 894
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 390, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 432, 569
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 180
Bse21I CCTNAGG 1 cut(s) 544
Bse8I GATNNNNATC 1 cut(s) 894
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 2 cut(s) 390, 426
BseGI GGATG 2 cut(s) 120, 539
BseJI GATNNNNATC 1 cut(s) 894
BseLI CCNNNNNNNGG 3 cut(s) 256, 432, 569
BseMII CTCAG 2 cut(s) 558, 869
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 2 cut(s) 326, 456
BseXI GCAGC 5 cut(s) 247, 332, 603, 763, 920
BseYI CCCAGC 1 cut(s) 340
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 4 cut(s) 161, 205, 249, 549
BsiHKAI GWGCWC 1 cut(s) 668
BsiHKCI CYCGRG 1 cut(s) 661
BsiSI CCGG 2 cut(s) 38, 402
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 3 cut(s) 256, 432, 569
BsmAI GTCTC 1 cut(s) 541
BsmBI CGTCTC 1 cut(s) 541
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 4 cut(s) 161, 205, 249, 549
BsoBI CYCGRG 1 cut(s) 661
Bsp1286I GDGCHC 1 cut(s) 668
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 2 cut(s) 689, 848
BspACI CCGC 3 cut(s) 158, 246, 264
BspANI GGCC 4 cut(s) 161, 205, 249, 549
BspCNI CTCAG 2 cut(s) 557, 868
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 424, 691
BspPI GGATC 3 cut(s) 684, 697, 856
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 1 cut(s) 180
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 390, 426
BssMI GATC 2 cut(s) 689, 848
BssNAI GTATAC 1 cut(s) 72
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 390
Bst1107I GTATAC 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 4 cut(s) 76, 427, 622, 925
Bst6I CTCTTC 2 cut(s) 191, 961
BstACI GRCGYC 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 600
BstAUI TGTACA 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 583
BstDEI CTNAG 2 cut(s) 544, 855
BstDSI CCRYGG 1 cut(s) 426
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 2 cut(s) 120, 539
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 2 cut(s) 298, 715
BstKTI GATC 2 cut(s) 692, 851
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 2 cut(s) 689, 848
BstMWI GCNNNNNNNGC 3 cut(s) 591, 600, 785
BstNI CCWGG 1 cut(s) 578
BstPI GGTNACC 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 576
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 5 cut(s) 247, 332, 603, 763, 920
BstX2I RGATCY 2 cut(s) 689, 848
BstYI RGATCY 2 cut(s) 689, 848
BstZ17I GTATAC 1 cut(s) 72
Bsu36I CCTNAGG 1 cut(s) 544
BsuRI GGCC 4 cut(s) 161, 205, 249, 549
BtgI CCRYGG 1 cut(s) 426
BtsCI GGATG 2 cut(s) 120, 539
BtsIMutI CAGTG 1 cut(s) 173
Cac8I GCNNGC 1 cut(s) 583
CciI TCATGA 1 cut(s) 13
CfoI GCGC 2 cut(s) 298, 715
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 3 cut(s) 423, 470, 489
CseI GACGC 1 cut(s) 523
Csp6I GTAC 1 cut(s) 91
CspCI CAANNNNNGTGG 4 cut(s) 298, 333, 481, 516
CviAII CATG 4 cut(s) 14, 685, 721, 826
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 2 cut(s) 544, 855
DpnI GATC 2 cut(s) 691, 850
DpnII GATC 2 cut(s) 689, 848
Eam1104I CTCTTC 2 cut(s) 191, 961
EarI CTCTTC 2 cut(s) 191, 961
Eco130I CCWWGG 1 cut(s) 390
Eco32I GATATC 1 cut(s) 647
Eco47I GGWCC 3 cut(s) 423, 470, 489
Eco47III AGCGCT 1 cut(s) 297
Eco81I CCTNAGG 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 661
Eco91I GGTNACC 1 cut(s) 55
EcoO65I GGTNACC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 576
EcoRV GATATC 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 4 cut(s) 17, 688, 724, 829
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 1 cut(s) 436
FatI CATG 4 cut(s) 13, 684, 720, 825
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 8 cut(s) 159, 247, 261, 264, 321, 592, 777, 934
FokI GGATG 2 cut(s) 127, 526
Fsp4HI GCNGC 8 cut(s) 159, 247, 261, 264, 321, 592, 777, 934
FspBI CTAG 3 cut(s) 98, 293, 528
GlaI GCGC 2 cut(s) 297, 714
GluI GCNGC 8 cut(s) 159, 247, 261, 264, 321, 592, 777, 934
GsaI CCCAGC 1 cut(s) 344
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 4 cut(s) 161, 205, 249, 549
HapII CCGG 2 cut(s) 38, 402
HgaI GACGC 1 cut(s) 523
HhaI GCGC 2 cut(s) 298, 715
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 4 cut(s) 17, 688, 724, 829
Hin6I GCGC 2 cut(s) 296, 713
HinP1I GCGC 2 cut(s) 296, 713
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HindIII AAGCTT 1 cut(s) 234
HinfI GANTC 4 cut(s) 169, 289, 736, 895
HpaII CCGG 2 cut(s) 38, 402
HphI GGTGA 2 cut(s) 52, 297
Hpy166II GTNNAC 6 cut(s) 72, 387, 470, 492, 771, 928
Hpy188I TCNGA 2 cut(s) 183, 608
Hpy188III TCNNGA 1 cut(s) 14
Hpy8I GTNNAC 6 cut(s) 72, 387, 470, 492, 771, 928
HpyAV CCTTC 3 cut(s) 195, 361, 515
HpyCH4III ACNGT 4 cut(s) 76, 427, 622, 925
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 7 cut(s) 7, 104, 320, 566, 681, 720, 779
HpyF10VI GCNNNNNNNGC 3 cut(s) 591, 600, 785
HpyF3I CTNAG 2 cut(s) 544, 855
HpySE526I ACGT 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 4 cut(s) 17, 688, 724, 829
HspAI GCGC 2 cut(s) 296, 713
Kzo9I GATC 2 cut(s) 689, 848
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 5 cut(s) 247, 332, 603, 763, 920
LweI GCATC 2 cut(s) 603, 690
MaeI CTAG 3 cut(s) 98, 293, 528
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 4 cut(s) 55, 106, 303, 944
MalI GATC 2 cut(s) 691, 850
MboI GATC 2 cut(s) 689, 848
MboII GAAGA 5 cut(s) 15, 208, 643, 757, 948
MflI RGATCY 2 cut(s) 689, 848
MhlI GDGCHC 1 cut(s) 668
MluCI AATT 4 cut(s) 28, 252, 414, 949
MlyI GAGTC 2 cut(s) 298, 745
MnlI CCTC 5 cut(s) 10, 186, 347, 434, 553
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 1 cut(s) 674
MspA1I CMGCKG 2 cut(s) 158, 933
MspI CCGG 2 cut(s) 38, 402
MspR9I CCNGG 1 cut(s) 578
MvaI CCWGG 1 cut(s) 578
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 3 cut(s) 591, 600, 785
NdeII GATC 2 cut(s) 689, 848
NlaIII CATG 4 cut(s) 17, 688, 724, 829
NlaIV GGNNCC 2 cut(s) 424, 691
NmeAIII GCCGAG 1 cut(s) 764
NmuCI GTSAC 1 cut(s) 303
PaeR7I CTCGAG 1 cut(s) 661
PagI TCATGA 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 169, 895
PkrI GCNGC 8 cut(s) 160, 248, 262, 265, 322, 593, 778, 935
PleI GAGTC 2 cut(s) 297, 744
PpsI GAGTC 2 cut(s) 297, 744
Psp6I CCWGG 1 cut(s) 576
PspEI GGTNACC 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 2 cut(s) 424, 691
PspPI GGNCC 3 cut(s) 423, 470, 489
PspXI VCTCGAGB 1 cut(s) 661
PsrI GAACNNNNNNTAC 2 cut(s) 74, 106
PsuI RGATCY 2 cut(s) 689, 848
PvuII CAGCTG 1 cut(s) 933
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SaqAI TTAA 1 cut(s) 674
SatI GCNGC 8 cut(s) 159, 247, 261, 264, 321, 592, 777, 934
Sau3AI GATC 2 cut(s) 689, 848
Sau96I GGNCC 3 cut(s) 423, 470, 489
SchI GAGTC 2 cut(s) 298, 745
ScrFI CCNGG 1 cut(s) 578
SduI GDGCHC 1 cut(s) 668
SfaNI GCATC 2 cut(s) 603, 690
Sfr274I CTCGAG 1 cut(s) 661
SinI GGWCC 3 cut(s) 423, 470, 489
SlaI CTCGAG 1 cut(s) 661
SmlI CTYRAG 3 cut(s) 188, 661, 820
SmoI CTYRAG 3 cut(s) 188, 661, 820
SpeI ACTAGT 2 cut(s) 97, 527
Sse9I AATT 4 cut(s) 28, 252, 414, 949
SsiI CCGC 3 cut(s) 158, 246, 264
SspMI CTAG 3 cut(s) 98, 293, 528
StyD4I CCNGG 1 cut(s) 576
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 4 cut(s) 76, 427, 622, 925
TaiI ACGT 1 cut(s) 305
TaqI TCGA 1 cut(s) 662
TasI AATT 4 cut(s) 28, 252, 414, 949
TatI WGTACW 1 cut(s) 90
TauI GCSGC 3 cut(s) 161, 249, 266
TfiI GAWTC 2 cut(s) 169, 895
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 303
TseI GCWGC 5 cut(s) 260, 320, 591, 776, 933
Tsp45I GTSAC 1 cut(s) 303
TspDTI ATGAA 2 cut(s) 95, 131
TspGWI ACGGA 3 cut(s) 691, 798, 850
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 3 cut(s) 423, 470, 489
XcmI CCANNNNNNNNNTGG 2 cut(s) 213, 352
XhoI CTCGAG 1 cut(s) 661
XmiI GTMKAC 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 3 cut(s) 98, 293, 528
ZraI GACGTC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.