Rh7CG397700

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000337
Physical Location & Seq
Forward (+)
50713679 .. 50716136
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG397700.1

Sequence Viewer

Length: 966 bp
ATGGGGAAGCAGAGTAGTGAGAGTAGTGTAGTTGATGGCTTAATGAAGCCAGTGATAACGATGGTGATCGCTCAGATTGCATATGCAGCAACGAGTATATTCTACAAGGTTGCAGCAGACTCTGGAATGAATCTCAGGATTCTCGTTGCCTACCGTCTCATGTTTTCATCTGCCATTATCGTTCCTCTTGCTCTTATACTCGAACGGAACAGCAGGCCCAAACTCACTCTAGTCGTACTCTTTCAAGCTTTTCTCTGCGGATTATTTGGGGGATCATTGTCAGAAAATTTATTCATAGAGGGATTAGTCTTAACATCACCAACCTTTGTTGCAGCCACTGCAAATCTGATTCCAGCCTTCACCTTCATCATGGCCATCTCTTTCAGGTTGGAGAAGTTGGCACTAGGAAGCCATGCAGGGAAGGCAAAGCTTGTGGGAACAGTGGTGGGTCTTGGTGGTGCTATGATCTTTACCTTCTTCAAAGGACCAGAGTTCTCCATCTGGTCAACTCACGTTGACCTTCTTCGAGGACATCAGGCGGCAACATCACCGTTATCGTCCTCCTCCCATAGAAGCACCGGGAGTCACCTGCTGGGCTCCTTCTTGGCACTCGGCTGCGTCGTTTCCTATGCAATGTGGCTCATAGTTCAGGCAAAAATGAGCAAGAGATATCCATGCCATTACTCGAGCACGGCTCTATTGTCGGTCATGGGATCGATTCAGTCTGTAGCTTTTGCTCTTTGCGTGGAGAGGGATTGGAGCCAGTGGAAATTGGGTTGGAACATCAAGCTTTTTACAGCTGCATATGGGGGAATTGTGACTTCGGGGATGGTGGTGGTTTTGATCTCTTGGTGTGTACGCAAGCGAGGTCCATTGTTTGTGTCAGTTTTCACTCCGCTTTCCCTAGTGATTGTGGCCATTGCTAGTTCTTTATTGCTCAATGAAAAGTTAAGCCTAGGCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

321

Amino Acids

34.54

Weight (kDa)

9.84

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 17 - 158 5.4e-11 EamA-like transporter family
EamA PF00892 198 - 319 1.1e-09 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 597
Acc36I ACCTGC 2 cut(s) 597, 951
AciI CCGC 3 cut(s) 258, 539, 896
AclWI GGATC 2 cut(s) 280, 721
AcoI YGGCCR 2 cut(s) 372, 915
AcsI RAATTY 1 cut(s) 286
AfaI GTAC 2 cut(s) 237, 858
AgsI TTSAA 2 cut(s) 245, 481
AluBI AGCT 5 cut(s) 248, 430, 731, 790, 800
AluI AGCT 5 cut(s) 248, 430, 731, 790, 800
Alw21I GWGCWC 1 cut(s) 692
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 2 cut(s) 280, 721
AlwNI CAGNNNCTG 2 cut(s) 122, 338
Ama87I CYCGRG 1 cut(s) 685
AoxI GGCC 3 cut(s) 215, 372, 915
ApeKI GCWGC 5 cut(s) 86, 113, 332, 615, 800
ApoI RAATTY 1 cut(s) 286
AspA2I CCTAGG 1 cut(s) 955
AspS9I GGNCC 3 cut(s) 216, 485, 869
AsuC2I CCSGG 1 cut(s) 580
AsuHPI GGTGA 5 cut(s) 76, 309, 352, 540, 578
AvaI CYCGRG 1 cut(s) 685
AvaII GGWCC 2 cut(s) 485, 869
AvrII CCTAGG 1 cut(s) 955
BalI TGGCCA 2 cut(s) 374, 917
BanII GRGCYC 1 cut(s) 599
Bbv12I GWGCWC 1 cut(s) 692
BbvI GCAGC 5 cut(s) 98, 125, 344, 602, 787
BccI CCATC 5 cut(s) 29, 55, 383, 506, 823
BceAI ACGGC 1 cut(s) 708
BcnI CCSGG 1 cut(s) 580
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 5 cut(s) 230, 404, 905, 924, 956
BfmI CTRYAG 1 cut(s) 726
BfuAI ACCTGC 2 cut(s) 597, 951
BisI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
BlnI CCTAGG 1 cut(s) 955
BlsI GCNGC 6 cut(s) 88, 115, 334, 541, 617, 802
Bme1390I CCNGG 1 cut(s) 580
Bme18I GGWCC 2 cut(s) 485, 869
BmeT110I CYCGRG 1 cut(s) 685
BmgT120I GGNCC 3 cut(s) 216, 485, 869
BmiI GGNNCC 2 cut(s) 598, 761
BmrFI CCNGG 1 cut(s) 580
BplI GAGNNNNNCTC 2 cut(s) 679, 711
BpuMI CCSGG 1 cut(s) 580
Bsa29I ATCGAT 1 cut(s) 716
BsaBI GATNNNNATC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 955
Bse1I ACTGG 2 cut(s) 50, 763
Bse3DI GCAATG 2 cut(s) 639, 918
Bse8I GATNNNNATC 1 cut(s) 65
BseCI ATCGAT 1 cut(s) 716
BseDI CCNNGG 1 cut(s) 955
BseGI GGATG 1 cut(s) 834
BseJI GATNNNNATC 1 cut(s) 65
BseMI GCAATG 2 cut(s) 639, 918
BseMII CTCAG 2 cut(s) 86, 148
BseNI ACTGG 2 cut(s) 50, 763
BseRI GAGGAG 1 cut(s) 553
BseXI GCAGC 5 cut(s) 98, 125, 344, 602, 787
BseYI CCCAGC 1 cut(s) 592
BshFI GGCC 3 cut(s) 217, 374, 917
BshVI ATCGAT 1 cut(s) 716
BsiHKAI GWGCWC 1 cut(s) 692
BsiHKCI CYCGRG 1 cut(s) 685
BsiSI CCGG 1 cut(s) 579
BsmAI GTCTC 1 cut(s) 161
BsmBI CGTCTC 1 cut(s) 161
BsnI GGCC 3 cut(s) 217, 374, 917
BsoBI CYCGRG 1 cut(s) 685
Bsp1286I GDGCHC 2 cut(s) 599, 692
Bsp143I GATC 5 cut(s) 66, 272, 465, 713, 843
BspACI CCGC 3 cut(s) 258, 539, 896
BspANI GGCC 3 cut(s) 217, 374, 917
BspCNI CTCAG 2 cut(s) 85, 147
BspDI ATCGAT 1 cut(s) 716
BspLI GGNNCC 2 cut(s) 598, 761
BspMI ACCTGC 2 cut(s) 597, 951
BspPI GGATC 2 cut(s) 280, 721
BsrDI GCAATG 2 cut(s) 639, 918
BsrI ACTGG 2 cut(s) 50, 763
BssECI CCNNGG 1 cut(s) 955
BssMI GATC 5 cut(s) 66, 272, 465, 713, 843
BssT1I CCWWGG 1 cut(s) 955
Bst4CI ACNGT 3 cut(s) 155, 442, 552
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 215, 863
BstDEI CTNAG 2 cut(s) 72, 134
BstF5I GGATG 1 cut(s) 834
BstKTI GATC 5 cut(s) 69, 275, 468, 716, 846
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 5 cut(s) 66, 272, 465, 713, 843
BstMWI GCNNNNNNNGC 4 cut(s) 77, 86, 338, 422
BstSCI CCNGG 1 cut(s) 578
BstSFI CTRYAG 1 cut(s) 726
BstV1I GCAGC 5 cut(s) 98, 125, 344, 602, 787
Bsu15I ATCGAT 1 cut(s) 716
BsuRI GGCC 3 cut(s) 217, 374, 917
BsuTUI ATCGAT 1 cut(s) 716
BtsCI GGATG 1 cut(s) 834
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 4 cut(s) 57, 336, 447, 770
BveI ACCTGC 2 cut(s) 597, 951
Cac8I GCNNGC 2 cut(s) 215, 863
CaiI CAGNNNCTG 2 cut(s) 122, 338
Cfr13I GGNCC 3 cut(s) 216, 485, 869
ClaI ATCGAT 1 cut(s) 716
CseI GACGC 1 cut(s) 607
Csp6I GTAC 2 cut(s) 236, 857
CspCI CAANNNNNGTGG 2 cut(s) 414, 449
CviAII CATG 5 cut(s) 160, 370, 413, 675, 709
CviQI GTAC 2 cut(s) 236, 857
DdeI CTNAG 2 cut(s) 72, 134
DpnI GATC 5 cut(s) 68, 274, 467, 715, 845
DpnII GATC 5 cut(s) 66, 272, 465, 713, 843
EaeI YGGCCR 2 cut(s) 372, 915
Eco130I CCWWGG 1 cut(s) 955
Eco24I GRGCYC 1 cut(s) 599
Eco32I GATATC 1 cut(s) 671
Eco47I GGWCC 2 cut(s) 485, 869
Eco88I CYCGRG 1 cut(s) 685
EcoRV GATATC 1 cut(s) 671
EcoT14I CCWWGG 1 cut(s) 955
EcoT38I GRGCYC 1 cut(s) 599
ErhI CCWWGG 1 cut(s) 955
Esp3I CGTCTC 1 cut(s) 161
FaeI CATG 5 cut(s) 163, 373, 416, 678, 712
FatI CATG 5 cut(s) 159, 369, 412, 674, 708
FauNDI CATATG 2 cut(s) 82, 805
Fnu4HI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
FokI GGATG 1 cut(s) 841
FriOI GRGCYC 1 cut(s) 599
Fsp4HI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
FspBI CTAG 5 cut(s) 230, 404, 905, 924, 956
GluI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
GsaI CCCAGC 1 cut(s) 596
HaeIII GGCC 3 cut(s) 217, 374, 917
HapII CCGG 1 cut(s) 579
HgaI GACGC 1 cut(s) 607
Hin1II CATG 5 cut(s) 163, 373, 416, 678, 712
HincII GTYRAC 2 cut(s) 507, 517
HindII GTYRAC 2 cut(s) 507, 517
HindIII AAGCTT 3 cut(s) 246, 428, 788
HinfI GANTC 6 cut(s) 119, 130, 139, 349, 583, 718
HpaII CCGG 1 cut(s) 579
HphI GGTGA 5 cut(s) 76, 309, 352, 540, 578
Hpy166II GTNNAC 3 cut(s) 507, 517, 857
Hpy188I TCNGA 3 cut(s) 75, 283, 348
Hpy188III TCNNGA 2 cut(s) 123, 136
Hpy8I GTNNAC 3 cut(s) 507, 517, 857
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 6 cut(s) 367, 373, 415, 484, 530, 610
HpyCH4III ACNGT 3 cut(s) 155, 442, 552
HpyCH4IV ACGT 1 cut(s) 513
HpyCH4V TGCA 8 cut(s) 80, 86, 113, 332, 341, 416, 632, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 86, 338, 422
HpyF3I CTNAG 2 cut(s) 72, 134
HpySE526I ACGT 1 cut(s) 513
Hsp92II CATG 5 cut(s) 163, 373, 416, 678, 712
Kzo9I GATC 5 cut(s) 66, 272, 465, 713, 843
LmnI GCTCC 2 cut(s) 602, 759
Lsp1109I GCAGC 5 cut(s) 98, 125, 344, 602, 787
MaeI CTAG 5 cut(s) 230, 404, 905, 924, 956
MaeII ACGT 1 cut(s) 513
MaeIII GTNAC 2 cut(s) 584, 817
MalI GATC 5 cut(s) 68, 274, 467, 715, 845
MboI GATC 5 cut(s) 66, 272, 465, 713, 843
MboII GAAGA 2 cut(s) 469, 515
MhlI GDGCHC 2 cut(s) 599, 692
MlsI TGGCCA 2 cut(s) 374, 917
MluCI AATT 3 cut(s) 286, 770, 813
MluNI TGGCCA 2 cut(s) 374, 917
MlyI GAGTC 2 cut(s) 113, 592
MmeI TCCRAC 2 cut(s) 369, 758
MnlI CCTC 7 cut(s) 195, 292, 521, 571, 574, 744, 860
Mox20I TGGCCA 2 cut(s) 374, 917
MscI TGGCCA 2 cut(s) 374, 917
MseI TTAA 3 cut(s) 41, 311, 950
Msp20I TGGCCA 2 cut(s) 374, 917
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 1 cut(s) 579
MspR9I CCNGG 1 cut(s) 580
MwoI GCNNNNNNNGC 4 cut(s) 77, 86, 338, 422
NciI CCSGG 1 cut(s) 580
NdeI CATATG 2 cut(s) 82, 805
NdeII GATC 5 cut(s) 66, 272, 465, 713, 843
NlaIII CATG 5 cut(s) 163, 373, 416, 678, 712
NlaIV GGNNCC 2 cut(s) 598, 761
NmeAIII GCCGAG 1 cut(s) 591
NmuCI GTSAC 2 cut(s) 584, 817
PaeR7I CTCGAG 1 cut(s) 685
PaqCI CACCTGC 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PfeI GAWTC 4 cut(s) 130, 139, 349, 718
PkrI GCNGC 6 cut(s) 88, 115, 334, 541, 617, 802
PleI GAGTC 2 cut(s) 113, 591
PpsI GAGTC 2 cut(s) 113, 591
PspFI CCCAGC 1 cut(s) 592
PspN4I GGNNCC 2 cut(s) 598, 761
PspPI GGNCC 3 cut(s) 216, 485, 869
PspXI VCTCGAGB 1 cut(s) 685
PstNI CAGNNNCTG 2 cut(s) 122, 338
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 2 cut(s) 237, 858
RsaNI GTAC 2 cut(s) 236, 857
SaqAI TTAA 3 cut(s) 41, 311, 950
SatI GCNGC 6 cut(s) 87, 114, 333, 540, 616, 801
Sau3AI GATC 5 cut(s) 66, 272, 465, 713, 843
Sau96I GGNCC 3 cut(s) 216, 485, 869
SchI GAGTC 2 cut(s) 113, 592
ScrFI CCNGG 1 cut(s) 580
SduI GDGCHC 2 cut(s) 599, 692
SfcI CTRYAG 1 cut(s) 726
Sfr274I CTCGAG 1 cut(s) 685
SinI GGWCC 2 cut(s) 485, 869
SlaI CTCGAG 1 cut(s) 685
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
Sse9I AATT 3 cut(s) 286, 770, 813
SsiI CCGC 3 cut(s) 258, 539, 896
SspMI CTAG 5 cut(s) 230, 404, 905, 924, 956
StyD4I CCNGG 1 cut(s) 578
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 3 cut(s) 155, 442, 552
TaiI ACGT 1 cut(s) 516
TaqI TCGA 4 cut(s) 201, 526, 686, 716
TaqII GACCGA 1 cut(s) 694
TasI AATT 3 cut(s) 286, 770, 813
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 4 cut(s) 130, 139, 349, 718
Tru1I TTAA 3 cut(s) 41, 311, 950
Tru9I TTAA 3 cut(s) 41, 311, 950
TscAI CASTG 4 cut(s) 57, 343, 447, 770
TseFI GTSAC 2 cut(s) 584, 817
TseI GCWGC 5 cut(s) 86, 113, 332, 615, 800
Tsp45I GTSAC 2 cut(s) 584, 817
TspDTI ATGAA 6 cut(s) 59, 143, 156, 283, 355, 957
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 4 cut(s) 57, 343, 447, 770
VpaK11BI GGWCC 2 cut(s) 485, 869
XapI RAATTY 1 cut(s) 286
XhoI CTCGAG 1 cut(s) 685
XmaJI CCTAGG 1 cut(s) 955
XspI CTAG 5 cut(s) 230, 404, 905, 924, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.