FvH4_7g27430

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000337
Physical Location & Seq
Forward (+)
20505168 .. 20507232
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27430.t1

Sequence Viewer

Length: 1107 bp
ATGGTGGTGCAGAATCATGAGGCTGCTTCTAATTATATGAAGGCGGTGATTATGATGGTGATTACCCAGATTTTGTGTTCTGTAATGATCATAATGTACAAGCTGGTTGCAGTGACTGGGGCGAACTTGAATGTTCTCGTTGCCTATCGACTCGTGTTTTCAGCTGCCTTTATGGTTCCACTGGCTCTGATATACGAACGGAAAAGCAGACCAAAACTTACTTGGGTTGTGTTGTTTCAAGCATTTCTCTGCGGCCTATTTGGGGCTGCTTTGTCACAAAACTTGTACATTGTGGGTCTCGCCCTCGCATCACCCACATTTCTTGCAGCCATCTCCAATCTCTCCCCAGCCATTACCTTCTTGGTTGCTCTATGTTTTCGGCAGGAGAAGTTGATCATGGGATTTTCTGGCATAGTCAAAATTGCTGGAACCGTGGTTGGAATAGGAGGAGCATTGCTTTTTACTTTCTACCGAGGACCTATATTTACCATTTGGTCCACTCATATTGACCTTCTACGGCAATTTCACAACAGTAGTAGCTTCTCATCATCATCCTCAAGCCACATGAACAATCCTGCATTGGGATTTCTGGCAGGCTTCGGCAGCATCTTGTGCTTCTCATTATGGCTCATAGTTCAGGGGAAGATGAGCAAAAGATATCCTTTCCCTTACTCGAGCACGGCTCTGATGTGCATCATGGGATCCATCCAGAACGTTATTTTCGCGCTTTGCGTGGAGAGAGATCGGAATCAATGGAAGATGGGTTGGGATATTAGGTTGTGGACAGCTGCATATTCGGGAATCGTGGTTTCCGGAGTGAGCATGGTTTTGATGTCTTGGTGTGTACAAAAGCGAGGGCCACTTTTTGTGTCAAGCTTTAATCCACTTACCCTGTTGATGGTAGCCCTTGCTAGTTCCTTGTTGTTGGGAGAAAAATTTAGCCTCGGAAGTATGTTAGGAGGAGTTCTAATTGTGTGCGGGTTATACATGGTGCTCTGGGGAAAAAGCAAAGAAGGATCATCATTGCTTGTACTACCCTCATCGACAATAGCTCCCAAGGATTCCGAGTTCATAGATAGCGTTCCGGTCCCGGCGAGTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

369

Amino Acids

40.01

Weight (kDa)

9.51

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 14 - 155 5.9e-11 EamA-like transporter family
EamA PF00892 194 - 332 1.1e-14 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 725
AccIII TCCGGA 1 cut(s) 812
AciI CCGC 3 cut(s) 44, 252, 978
AclI AACGTT 1 cut(s) 714
AclWI GGATC 3 cut(s) 696, 709, 1024
AcsI RAATTY 1 cut(s) 935
AfaI GTAC 4 cut(s) 98, 287, 846, 1032
AfiI CCNNNNNNNGG 3 cut(s) 262, 581, 898
AgsI TTSAA 2 cut(s) 130, 239
AloI GAACNNNNNNTCC 2 cut(s) 1052, 1084
AluBI AGCT 6 cut(s) 103, 164, 540, 788, 876, 1052
AluI AGCT 6 cut(s) 103, 164, 540, 788, 876, 1052
Alw21I GWGCWC 2 cut(s) 680, 996
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 3 cut(s) 696, 709, 1024
AlwNI CAGNNNCTG 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 673
Aor13HI TCCGGA 1 cut(s) 812
AoxI GGCC 2 cut(s) 253, 857
ApeKI GCWGC 6 cut(s) 23, 164, 266, 326, 603, 788
ApoI RAATTY 1 cut(s) 935
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 4 cut(s) 476, 495, 857, 1087
AsuC2I CCSGG 1 cut(s) 1091
AsuHPI GGTGA 3 cut(s) 58, 70, 303
AvaI CYCGRG 1 cut(s) 673
AvaII GGWCC 3 cut(s) 476, 495, 1087
BamHI GGATCC 1 cut(s) 701
BauI CACGAG 1 cut(s) 152
Bbv12I GWGCWC 2 cut(s) 680, 996
BbvI GCAGC 6 cut(s) 10, 151, 253, 338, 615, 775
BccI CCATC 5 cut(s) 49, 338, 713, 754, 892
BceAI ACGGC 2 cut(s) 533, 696
BclI TGATCA 2 cut(s) 87, 393
BcnI CCSGG 1 cut(s) 1091
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 1 cut(s) 912
BisI GCNGC 7 cut(s) 24, 165, 253, 267, 327, 604, 789
BlsI GCNGC 7 cut(s) 25, 166, 254, 268, 328, 605, 790
Bme1390I CCNGG 1 cut(s) 1091
Bme18I GGWCC 3 cut(s) 476, 495, 1087
BmeT110I CYCGRG 1 cut(s) 673
BmgT120I GGNCC 4 cut(s) 476, 495, 857, 1087
BmiI GGNNCC 4 cut(s) 177, 430, 703, 1089
BmrFI CCNGG 1 cut(s) 1091
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 3 cut(s) 317, 615, 702
BmuI ACTGGG 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 667, 699
BpuEI CTTGAG 1 cut(s) 541
BpuMI CCSGG 1 cut(s) 1091
BsaBI GATNNNNATC 2 cut(s) 692, 747
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 4 cut(s) 432, 472, 943, 1056
BsaWI WCCGGW 2 cut(s) 812, 1084
BsaXI ACNNNNNCTCC 2 cut(s) 1036, 1066
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 581, 898
Bse1I ACTGG 2 cut(s) 121, 186
Bse3DI GCAATG 2 cut(s) 452, 1022
Bse8I GATNNNNATC 2 cut(s) 692, 747
BseAI TCCGGA 1 cut(s) 812
BseDI CCNNGG 4 cut(s) 432, 472, 943, 1056
BseGI GGATG 2 cut(s) 551, 705
BseJI GATNNNNATC 2 cut(s) 692, 747
BseLI CCNNNNNNNGG 3 cut(s) 262, 581, 898
BseMI GCAATG 2 cut(s) 452, 1022
BseNI ACTGG 2 cut(s) 121, 186
BseRI GAGGAG 2 cut(s) 462, 975
BseXI GCAGC 6 cut(s) 10, 151, 253, 338, 615, 775
BseYI CCCAGC 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 29
Bsh1236I CGCG 1 cut(s) 725
BshFI GGCC 2 cut(s) 255, 859
BsiHKAI GWGCWC 2 cut(s) 680, 996
BsiHKCI CYCGRG 1 cut(s) 673
BsiSI CCGG 3 cut(s) 813, 1085, 1091
BslFI GGGAC 1 cut(s) 1073
BslI CCNNNNNNNGG 3 cut(s) 262, 581, 898
BsmAI GTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 1073
BsnI GGCC 2 cut(s) 255, 859
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 673
Bsp1286I GDGCHC 2 cut(s) 680, 996
Bsp13I TCCGGA 1 cut(s) 812
Bsp1407I TGTACA 3 cut(s) 96, 285, 844
Bsp143I GATC 5 cut(s) 87, 393, 701, 742, 1016
BspACI CCGC 3 cut(s) 44, 252, 978
BspANI GGCC 2 cut(s) 255, 859
BspEI TCCGGA 1 cut(s) 812
BspFNI CGCG 1 cut(s) 725
BspHI TCATGA 1 cut(s) 16
BspLI GGNNCC 4 cut(s) 177, 430, 703, 1089
BspPI GGATC 3 cut(s) 696, 709, 1024
BspTNI GGTCTC 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 452, 1022
BsrGI TGTACA 3 cut(s) 96, 285, 844
BsrI ACTGG 2 cut(s) 121, 186
BssECI CCNNGG 4 cut(s) 432, 472, 943, 1056
BssMI GATC 5 cut(s) 87, 393, 701, 742, 1016
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 1056
Bst2BI CACGAG 1 cut(s) 152
Bst4CI ACNGT 2 cut(s) 433, 533
BstAPI GCANNNNNTGC 1 cut(s) 612
BstAUI TGTACA 3 cut(s) 96, 285, 844
BstC8I GCNNGC 1 cut(s) 595
BstDSI CCRYGG 1 cut(s) 432
BstF5I GGATG 2 cut(s) 551, 705
BstFNI CGCG 1 cut(s) 725
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 5 cut(s) 90, 396, 704, 745, 1019
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 5 cut(s) 87, 393, 701, 742, 1016
BstMWI GCNNNNNNNGC 2 cut(s) 603, 612
BstSCI CCNGG 1 cut(s) 1089
BstUI CGCG 1 cut(s) 725
BstV1I GCAGC 6 cut(s) 10, 151, 253, 338, 615, 775
BstX2I RGATCY 1 cut(s) 701
BstYI RGATCY 1 cut(s) 701
BsuRI GGCC 2 cut(s) 255, 859
BtgI CCRYGG 1 cut(s) 432
BtsCI GGATG 2 cut(s) 551, 705
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 117, 179
Cac8I GCNNGC 1 cut(s) 595
CaiI CAGNNNCTG 1 cut(s) 116
CciI TCATGA 1 cut(s) 16
CfoI GCGC 1 cut(s) 727
Cfr13I GGNCC 4 cut(s) 476, 495, 857, 1087
Csp6I GTAC 4 cut(s) 97, 286, 845, 1031
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 487, 522
CviAII CATG 6 cut(s) 17, 397, 565, 697, 823, 988
CviQI GTAC 4 cut(s) 97, 286, 845, 1031
DpnI GATC 5 cut(s) 89, 395, 703, 744, 1018
DpnII GATC 5 cut(s) 87, 393, 701, 742, 1016
Eco130I CCWWGG 1 cut(s) 1056
Eco31I GGTCTC 1 cut(s) 302
Eco32I GATATC 1 cut(s) 659
Eco47I GGWCC 3 cut(s) 476, 495, 1087
Eco88I CYCGRG 1 cut(s) 673
EcoO109I RGGNCCY 1 cut(s) 476
EcoRV GATATC 1 cut(s) 659
EcoT14I CCWWGG 1 cut(s) 1056
ErhI CCWWGG 1 cut(s) 1056
FaeI CATG 6 cut(s) 20, 400, 568, 700, 826, 991
FalI AAGNNNNNCTT 2 cut(s) 646, 678
FaqI GGGAC 1 cut(s) 1073
FatI CATG 6 cut(s) 16, 396, 564, 696, 822, 987
FauI CCCGC 1 cut(s) 971
FbaI TGATCA 2 cut(s) 87, 393
Fnu4HI GCNGC 7 cut(s) 24, 165, 253, 267, 327, 604, 789
FokI GGATG 2 cut(s) 538, 692
Fsp4HI GCNGC 7 cut(s) 24, 165, 253, 267, 327, 604, 789
FspBI CTAG 1 cut(s) 912
GlaI GCGC 1 cut(s) 726
GluI GCNGC 7 cut(s) 24, 165, 253, 267, 327, 604, 789
GsaI CCCAGC 1 cut(s) 350
HaeIII GGCC 2 cut(s) 255, 859
HapII CCGG 3 cut(s) 813, 1085, 1091
HhaI GCGC 1 cut(s) 727
Hin1II CATG 6 cut(s) 20, 400, 568, 700, 826, 991
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HindIII AAGCTT 1 cut(s) 874
HinfI GANTC 5 cut(s) 13, 150, 748, 801, 1061
HpaII CCGG 3 cut(s) 813, 1085, 1091
HphI GGTGA 3 cut(s) 58, 70, 303
Hpy166II GTNNAC 3 cut(s) 498, 783, 845
Hpy188I TCNGA 5 cut(s) 189, 687, 747, 947, 1066
Hpy188III TCNNGA 4 cut(s) 17, 709, 798, 813
Hpy8I GTNNAC 3 cut(s) 498, 783, 845
HpyAV CCTTC 4 cut(s) 34, 367, 521, 1007
HpyCH4III ACNGT 2 cut(s) 433, 533
HpyCH4IV ACGT 1 cut(s) 714
HpyCH4V TGCA 6 cut(s) 10, 110, 326, 578, 693, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 603, 612
HpySE526I ACGT 1 cut(s) 714
Hsp92II CATG 6 cut(s) 20, 400, 568, 700, 826, 991
HspAI GCGC 1 cut(s) 725
Kpn2I TCCGGA 1 cut(s) 812
Ksp22I TGATCA 2 cut(s) 87, 393
Kzo9I GATC 5 cut(s) 87, 393, 701, 742, 1016
LmnI GCTCC 2 cut(s) 449, 1057
Lsp1109I GCAGC 6 cut(s) 10, 151, 253, 338, 615, 775
LweI GCATC 3 cut(s) 317, 615, 702
MaeI CTAG 1 cut(s) 912
MaeII ACGT 1 cut(s) 714
MaeIII GTNAC 2 cut(s) 112, 273
MalI GATC 5 cut(s) 89, 395, 703, 744, 1018
MboI GATC 5 cut(s) 87, 393, 701, 742, 1016
MboII GAAGA 2 cut(s) 655, 769
MflI RGATCY 1 cut(s) 701
MhlI GDGCHC 2 cut(s) 680, 996
MluCI AATT 5 cut(s) 31, 420, 521, 935, 969
MlyI GAGTC 1 cut(s) 144
MmeI TCCRAC 2 cut(s) 418, 1081
MnlI CCTC 9 cut(s) 13, 314, 440, 467, 565, 848, 953, 953, 1048
MroI TCCGGA 1 cut(s) 812
MseI TTAA 1 cut(s) 879
MspA1I CMGCKG 2 cut(s) 164, 788
MspI CCGG 3 cut(s) 813, 1085, 1091
MspR9I CCNGG 1 cut(s) 1091
MvnI CGCG 1 cut(s) 725
MwoI GCNNNNNNNGC 2 cut(s) 603, 612
NciI CCSGG 1 cut(s) 1091
NdeII GATC 5 cut(s) 87, 393, 701, 742, 1016
NlaIII CATG 6 cut(s) 20, 400, 568, 700, 826, 991
NlaIV GGNNCC 4 cut(s) 177, 430, 703, 1089
NmuCI GTSAC 2 cut(s) 112, 273
PaeR7I CTCGAG 1 cut(s) 673
PagI TCATGA 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 729
PfeI GAWTC 4 cut(s) 13, 748, 801, 1061
PkrI GCNGC 7 cut(s) 25, 166, 254, 268, 328, 605, 790
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
PpuMI RGGWCCY 1 cut(s) 476
Psp1406I AACGTT 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 476
PspFI CCCAGC 1 cut(s) 346
PspN4I GGNNCC 4 cut(s) 177, 430, 703, 1089
PspPI GGNCC 4 cut(s) 476, 495, 857, 1087
PspPPI RGGWCCY 1 cut(s) 476
PspXI VCTCGAGB 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 701
PvuII CAGCTG 2 cut(s) 164, 788
RsaI GTAC 4 cut(s) 98, 287, 846, 1032
RsaNI GTAC 4 cut(s) 97, 286, 845, 1031
SaqAI TTAA 1 cut(s) 879
SatI GCNGC 7 cut(s) 24, 165, 253, 267, 327, 604, 789
Sau3AI GATC 5 cut(s) 87, 393, 701, 742, 1016
Sau96I GGNCC 4 cut(s) 476, 495, 857, 1087
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 1 cut(s) 1091
SduI GDGCHC 2 cut(s) 680, 996
SfaNI GCATC 3 cut(s) 317, 615, 702
Sfr274I CTCGAG 1 cut(s) 673
SinI GGWCC 3 cut(s) 476, 495, 1087
SlaI CTCGAG 1 cut(s) 673
SmlI CTYRAG 2 cut(s) 556, 673
SmoI CTYRAG 2 cut(s) 556, 673
Sse9I AATT 5 cut(s) 31, 420, 521, 935, 969
SsiI CCGC 3 cut(s) 44, 252, 978
SspMI CTAG 1 cut(s) 912
StyD4I CCNGG 1 cut(s) 1089
StyI CCWWGG 1 cut(s) 1056
TaaI ACNGT 2 cut(s) 433, 533
TaiI ACGT 1 cut(s) 717
TaqI TCGA 3 cut(s) 148, 674, 1043
TasI AATT 5 cut(s) 31, 420, 521, 935, 969
TatI WGTACW 4 cut(s) 96, 285, 844, 1030
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 4 cut(s) 13, 748, 801, 1061
Tru1I TTAA 1 cut(s) 879
Tru9I TTAA 1 cut(s) 879
TscAI CASTG 2 cut(s) 117, 186
TseFI GTSAC 2 cut(s) 112, 273
TseI GCWGC 6 cut(s) 23, 164, 266, 326, 603, 788
Tsp45I GTSAC 2 cut(s) 112, 273
TspDTI ATGAA 3 cut(s) 53, 581, 1060
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 2 cut(s) 117, 186
VpaK11BI GGWCC 3 cut(s) 476, 495, 1087
XapI RAATTY 1 cut(s) 935
XcmI CCANNNNNNNNNTGG 2 cut(s) 219, 358
XhoI CTCGAG 1 cut(s) 673
XspI CTAG 1 cut(s) 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.