MD08G1224100.v1.1

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000337
Physical Location & Seq
Forward (+)
28849259 .. 28851271
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1224100.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGTGTTGCAGTACTAGTATAGTGCAAGGGTTGAAGCCAGTGATGATGATGCTGGTGGTTCAGGCGGCATATGTAGGGATGAATATATCTTACAAACTGGTAGCAGAGGTTGGAATGAATTTCACCGTCCTTGTTGCCTACCGTAACATGTTTTCTGCAGCCCTTATGCTTCCTCTTGCCCTTATATTTGAAAGGAACAGGCCCAAACTTACCTTAGGTATCCTGTTTCAAGGTTTTATCTCCGGATTATTGGGGGCAACCATGGCACAAAACTTTTACATAGAGAGCTTAGGCTTAACATCGGCAACATATGCCGTAGCCTTCAATAATCTTATTCCAGTCATCACGTTCCTCATAGCATTATGTTTTAGAATGGAGAAGTTAGCCATTGGGACACGCACGGGGAGGGCAAAAGTTGTGGGTATATTGGTTTGTGTAGGAGGCACAATGATCTTCACTTTCTATAAAGGCCACGAGATTGTCATTTGGTCGACGGACGTAAACCTTTTACGTAAATATTCCCACCAAAACAGCCACGTCTTGCACAGAAACACTCGCACTCAGTTGCTGGGCTCCGTCTTAGCACTCTGCAGTAGCATCTCTTTTGCACTGTGGCTGATATTTCAGAAGAAAATGACTAGGGATTACCCTTGCCAGTACTCAAGTACGGCCATGATGTCTATCATGGCATCAATCCAGTCCACTGCTTTTGCCCTCTGCAGGGAGAGGGACTGGAGTCAGTGGAAGTTGGGCTGGGACATCAAACTTTTCACGGCAGTGTATTCGGGAGTCATTACTTCGGGATTGGTTCTTACTCTGACCGCATGGTGTGTGCGCATGCGAGGGCCTTTGTTTGTGGCTATTTTCAACCCTCTTGGCCTTCTGCTGGTGGGCTTAGCTGCGACGTTGTTGTTAGATGAGAAGTTACACTTAGGAAGCATATTAGGAGGGGTTTTAATCATCTGTGGGTTATATATGGTTTTGTGGACTAAAAGAGAGGAACTGAAGACGATGACAGAACAGCCACCATCAGTACTTGAAGAGTCCCATTCTATTAGCAACATCGCCAGTGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

360

Amino Acids

39.82

Weight (kDa)

9.29

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 14 - 152 2.8e-10 EamA-like transporter family
EamA PF00892 190 - 328 1e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 836
AccI GTMKAC 1 cut(s) 491
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 2 cut(s) 65, 822
AcoI YGGCCR 1 cut(s) 669
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 1025
AfaI GTAC 4 cut(s) 13, 659, 667, 1035
AfiI CCNNNNNNNGG 3 cut(s) 720, 721, 886
AflIII ACRYGT 1 cut(s) 147
AgsI TTSAA 6 cut(s) 34, 191, 230, 325, 868, 1040
AhlI ACTAGT 1 cut(s) 14
AjiI CACGTC 1 cut(s) 538
AleI CACNNNNGTG 1 cut(s) 776
AluBI AGCT 2 cut(s) 288, 899
AluI AGCT 2 cut(s) 288, 899
AlwNI CAGNNNCTG 1 cut(s) 568
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 5 cut(s) 200, 469, 669, 845, 877
ApeKI GCWGC 2 cut(s) 158, 899
ApoI RAATTY 1 cut(s) 118
AspLEI GCGC 1 cut(s) 837
AspS9I GGNCC 2 cut(s) 201, 845
AsuHPI GGTGA 1 cut(s) 115
AxyI CCTNAGG 1 cut(s) 214
BanII GRGCYC 1 cut(s) 575
BauI CACGAG 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 1013
BbvI GCAGC 2 cut(s) 170, 886
BccI CCATC 1 cut(s) 1036
BceAI ACGGC 3 cut(s) 299, 684, 789
BcgI CGANNNNNNTGC 2 cut(s) 765, 799
BciVI GTATCC 1 cut(s) 230
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 2 cut(s) 15, 639
BfmI CTRYAG 3 cut(s) 156, 589, 718
BfuI GTATCC 1 cut(s) 230
BisI GCNGC 3 cut(s) 66, 159, 900
BlpI GCTNAGC 1 cut(s) 895
BlsI GCNGC 3 cut(s) 67, 160, 901
BmcAI AGTACT 3 cut(s) 13, 659, 1035
BmgBI CACGTC 1 cut(s) 538
BmgT120I GGNCC 2 cut(s) 201, 845
BmiI GGNNCC 1 cut(s) 574
BmsI GCATC 3 cut(s) 39, 606, 698
BoxI GACNNNNGTC 1 cut(s) 735
BpiI GAAGAC 1 cut(s) 1013
BpmI CTGGAG 1 cut(s) 754
Bpu10I CCTNAGC 1 cut(s) 289
Bpu1102I GCTNAGC 1 cut(s) 895
BpuEI CTTGAG 1 cut(s) 646
BsaAI YACGTR 1 cut(s) 512
BsaBI GATNNNNATC 1 cut(s) 680
BsaJI CCNNGG 1 cut(s) 261
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 3 cut(s) 720, 721, 886
Bse1I ACTGG 7 cut(s) 38, 102, 338, 655, 697, 737, 1068
Bse21I CCTNAGG 1 cut(s) 214
Bse8I GATNNNNATC 1 cut(s) 680
BseAI TCCGGA 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 1 cut(s) 84
BseJI GATNNNNATC 1 cut(s) 680
BseLI CCNNNNNNNGG 3 cut(s) 720, 721, 886
BseMII CTCAG 1 cut(s) 575
BseNI ACTGG 7 cut(s) 38, 102, 338, 655, 697, 737, 1068
BseXI GCAGC 2 cut(s) 170, 886
BseYI CCCAGC 2 cut(s) 568, 753
BshFI GGCC 5 cut(s) 202, 471, 671, 847, 879
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 4 cut(s) 406, 743, 770, 1030
BslI CCNNNNNNNGG 3 cut(s) 720, 721, 886
BsmFI GGGAC 4 cut(s) 406, 743, 770, 1030
BsnI GGCC 5 cut(s) 202, 471, 671, 847, 879
Bsp1286I GDGCHC 1 cut(s) 575
Bsp13I TCCGGA 1 cut(s) 242
Bsp143I GATC 1 cut(s) 450
Bsp1720I GCTNAGC 1 cut(s) 895
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 2 cut(s) 65, 822
BspANI GGCC 5 cut(s) 202, 471, 671, 847, 879
BspCNI CTCAG 1 cut(s) 574
BspEI TCCGGA 1 cut(s) 242
BspLI GGNNCC 1 cut(s) 574
BspMAI CTGCAG 3 cut(s) 160, 593, 722
BsrI ACTGG 7 cut(s) 38, 102, 338, 655, 697, 737, 1068
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 1 cut(s) 450
BssSI CACGAG 1 cut(s) 473
BssT1I CCWWGG 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 473
Bst4CI ACNGT 3 cut(s) 127, 143, 612
Bst6I CTCTTC 1 cut(s) 1035
BstAPI GCANNNNNTGC 1 cut(s) 311
BstBAI YACGTR 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 839
BstDEI CTNAG 6 cut(s) 214, 289, 561, 580, 895, 931
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 837
BstKTI GATC 1 cut(s) 453
BstMBI GATC 1 cut(s) 450
BstMWI GCNNNNNNNGC 2 cut(s) 263, 311
BstNSI RCATGY 2 cut(s) 151, 841
BstPAI GACNNNNGTC 1 cut(s) 735
BstSFI CTRYAG 3 cut(s) 156, 589, 718
BstSNI TACGTA 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 170, 886
BstV2I GAAGAC 1 cut(s) 1013
Bsu36I CCTNAGG 1 cut(s) 214
BsuI GTATCC 1 cut(s) 230
BsuRI GGCC 5 cut(s) 202, 471, 671, 847, 879
BtgI CCRYGG 1 cut(s) 261
BtgZI GCGATG 1 cut(s) 1048
BtrI CACGTC 1 cut(s) 538
BtsCI GGATG 1 cut(s) 84
BtsI GCAGTG 2 cut(s) 702, 783
BtsIMutI CAGTG 6 cut(s) 45, 608, 702, 746, 783, 1075
Cac8I GCNNGC 1 cut(s) 839
CaiI CAGNNNCTG 1 cut(s) 568
CfoI GCGC 1 cut(s) 837
Cfr13I GGNCC 2 cut(s) 201, 845
Csp6I GTAC 4 cut(s) 12, 658, 666, 1034
CspCI CAANNNNNGTGG 4 cut(s) 399, 434, 691, 726
CviAII CATG 6 cut(s) 148, 262, 673, 685, 825, 838
CviQI GTAC 4 cut(s) 12, 658, 666, 1034
DdeI CTNAG 6 cut(s) 214, 289, 561, 580, 895, 931
DpnI GATC 1 cut(s) 452
DpnII GATC 1 cut(s) 450
EaeI YGGCCR 1 cut(s) 669
Eam1104I CTCTTC 1 cut(s) 1035
EarI CTCTTC 1 cut(s) 1035
Eco105I TACGTA 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 1025
Eco81I CCTNAGG 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 845
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 575
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 6 cut(s) 151, 265, 676, 688, 828, 841
FalI AAGNNNNNCTT 2 cut(s) 914, 946
FaqI GGGAC 4 cut(s) 406, 743, 770, 1030
FatI CATG 6 cut(s) 147, 261, 672, 684, 824, 837
FauNDI CATATG 2 cut(s) 70, 310
FblI GTMKAC 1 cut(s) 491
Fnu4HI GCNGC 3 cut(s) 66, 159, 900
FokI GGATG 1 cut(s) 91
FriOI GRGCYC 1 cut(s) 575
Fsp4HI GCNGC 3 cut(s) 66, 159, 900
FspAI RTGCGCAY 1 cut(s) 836
FspBI CTAG 2 cut(s) 15, 639
FspI TGCGCA 1 cut(s) 836
GlaI GCGC 1 cut(s) 836
GluI GCNGC 3 cut(s) 66, 159, 900
GsaI CCCAGC 2 cut(s) 572, 757
GsuI CTGGAG 1 cut(s) 754
HaeIII GGCC 5 cut(s) 202, 471, 671, 847, 879
HapII CCGG 1 cut(s) 243
HhaI GCGC 1 cut(s) 837
Hin1II CATG 6 cut(s) 151, 265, 676, 688, 828, 841
Hin6I GCGC 1 cut(s) 835
HinP1I GCGC 1 cut(s) 835
HincII GTYRAC 1 cut(s) 492
HindII GTYRAC 1 cut(s) 492
HinfI GANTC 3 cut(s) 736, 789, 1043
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 4 cut(s) 492, 502, 702, 987
Hpy188I TCNGA 2 cut(s) 627, 819
Hpy188III TCNNGA 3 cut(s) 243, 786, 801
Hpy8I GTNNAC 4 cut(s) 492, 502, 702, 987
Hpy99I CGWCG 2 cut(s) 496, 907
HpyAV CCTTC 2 cut(s) 331, 890
HpyCH4III ACNGT 3 cut(s) 127, 143, 612
HpyCH4IV ACGT 5 cut(s) 347, 498, 511, 537, 905
HpyCH4V TGCA 7 cut(s) 9, 25, 158, 544, 591, 608, 720
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 311
HpyF3I CTNAG 6 cut(s) 214, 289, 561, 580, 895, 931
HpySE526I ACGT 5 cut(s) 347, 498, 511, 537, 905
Hsp92II CATG 6 cut(s) 151, 265, 676, 688, 828, 841
HspAI GCGC 1 cut(s) 835
Kpn2I TCCGGA 1 cut(s) 242
Kzo9I GATC 1 cut(s) 450
LmnI GCTCC 1 cut(s) 578
Lsp1109I GCAGC 2 cut(s) 170, 886
LweI GCATC 3 cut(s) 39, 606, 698
MaeI CTAG 2 cut(s) 15, 639
MaeII ACGT 5 cut(s) 347, 498, 511, 537, 905
MaeIII GTNAC 2 cut(s) 143, 924
MalI GATC 1 cut(s) 452
MboI GATC 1 cut(s) 450
MboII GAAGA 4 cut(s) 445, 640, 1018, 1052
MhlI GDGCHC 1 cut(s) 575
MluCI AATT 1 cut(s) 118
MlyI GAGTC 3 cut(s) 745, 798, 1052
MmeI TCCRAC 1 cut(s) 91
MroI TCCGGA 1 cut(s) 242
MseI TTAA 2 cut(s) 296, 956
MslI CAYNNNNRTG 1 cut(s) 776
MspI CCGG 1 cut(s) 243
MwoI GCNNNNNNNGC 2 cut(s) 263, 311
NcoI CCATGG 1 cut(s) 261
NdeI CATATG 2 cut(s) 70, 310
NdeII GATC 1 cut(s) 450
NlaIII CATG 6 cut(s) 151, 265, 676, 688, 828, 841
NlaIV GGNNCC 1 cut(s) 574
NsbI TGCGCA 1 cut(s) 836
NspI RCATGY 2 cut(s) 151, 841
OliI CACNNNNGTG 1 cut(s) 776
PaeI GCATGC 1 cut(s) 841
PciI ACATGT 1 cut(s) 147
PkrI GCNGC 3 cut(s) 67, 160, 901
PleI GAGTC 3 cut(s) 744, 797, 1051
PpsI GAGTC 3 cut(s) 744, 797, 1051
Ppu21I YACGTR 1 cut(s) 512
PscI ACATGT 1 cut(s) 147
PshAI GACNNNNGTC 1 cut(s) 735
PspFI CCCAGC 2 cut(s) 568, 753
PspN4I GGNNCC 1 cut(s) 574
PspPI GGNCC 2 cut(s) 201, 845
PstI CTGCAG 3 cut(s) 160, 593, 722
PstNI CAGNNNCTG 1 cut(s) 568
RsaI GTAC 4 cut(s) 13, 659, 667, 1035
RsaNI GTAC 4 cut(s) 12, 658, 666, 1034
RseI CAYNNNNRTG 1 cut(s) 776
SalI GTCGAC 1 cut(s) 490
SaqAI TTAA 2 cut(s) 296, 956
SatI GCNGC 3 cut(s) 66, 159, 900
Sau3AI GATC 1 cut(s) 450
Sau96I GGNCC 2 cut(s) 201, 845
ScaI AGTACT 3 cut(s) 13, 659, 1035
SchI GAGTC 3 cut(s) 745, 798, 1052
SduI GDGCHC 1 cut(s) 575
SfaNI GCATC 3 cut(s) 39, 606, 698
SfcI CTRYAG 3 cut(s) 156, 589, 718
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 661
SmoI CTYRAG 1 cut(s) 661
SnaBI TACGTA 1 cut(s) 512
SpeI ACTAGT 1 cut(s) 14
SphI GCATGC 1 cut(s) 841
Sse9I AATT 1 cut(s) 118
SsiI CCGC 2 cut(s) 65, 822
SspI AATATT 1 cut(s) 518
SspMI CTAG 2 cut(s) 15, 639
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 3 cut(s) 127, 143, 612
TaiI ACGT 5 cut(s) 350, 501, 514, 540, 908
TaqI TCGA 1 cut(s) 491
TasI AATT 1 cut(s) 118
TatI WGTACW 3 cut(s) 11, 657, 1033
TauI GCSGC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 296, 956
Tru9I TTAA 2 cut(s) 296, 956
TscAI CASTG 6 cut(s) 45, 615, 709, 746, 783, 1075
TseI GCWGC 2 cut(s) 158, 899
TspDTI ATGAA 2 cut(s) 95, 131
TspGWI ACGGA 2 cut(s) 509, 565
TspRI CASTG 6 cut(s) 45, 615, 709, 746, 783, 1075
XapI RAATTY 1 cut(s) 118
XceI RCATGY 2 cut(s) 151, 841
XmiI GTMKAC 1 cut(s) 491
XspI CTAG 2 cut(s) 15, 639
ZrmI AGTACT 3 cut(s) 13, 659, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.