Rmu_co8280549.1_g000001

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8280549.1
OG0000337
Physical Location & Seq
Forward (+)
90 .. 635
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8280549.1_g000001.1.cds

Sequence Viewer

Length: 456 bp
atgcacaggttggagaagttggcactaggaagccatgcagggaaggcaaagcttgtggggacagtggtgggtcttggtggtgctatgatctttaccttcttcaaaggaccagagttctccatctggtcaactcacgttgaccttcttcgaggacatcaggcggcaacatcaccgttatcgtcctcctcccatagaagcaccgggagtcacctgctgggctccttcttggcactcggctgcgtcgtttcctatgcaatgtggctcatagttcaggcaaaaatgagcaagagatatccatgccattactcgagcacggctctattgtcggtcatgggatcgattcagtctgtagcttttgctctttgcatggagagggattggagccagtggaaattgggttggaacatcaagctttttacagctgcatatggggtaaaatcactgctcattgattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

151

Amino Acids

16.45

Weight (kDa)

9.72

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 219
Acc36I ACCTGC 1 cut(s) 219
AciI CCGC 1 cut(s) 161
AclWI GGATC 1 cut(s) 343
AgsI TTSAA 1 cut(s) 103
AluBI AGCT 4 cut(s) 52, 353, 412, 422
AluI AGCT 4 cut(s) 52, 353, 412, 422
Alw21I GWGCWC 1 cut(s) 314
AlwI GGATC 1 cut(s) 343
Ama87I CYCGRG 1 cut(s) 307
ApeKI GCWGC 2 cut(s) 237, 422
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 162, 200
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 2 cut(s) 224, 409
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 330
BcnI CCSGG 1 cut(s) 202
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 348
BfuAI ACCTGC 1 cut(s) 219
BisI GCNGC 3 cut(s) 162, 238, 423
BlsI GCNGC 3 cut(s) 163, 239, 424
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 2 cut(s) 220, 383
BmrFI CCNGG 1 cut(s) 202
BplI GAGNNNNNCTC 2 cut(s) 301, 333
BpuMI CCSGG 1 cut(s) 202
Bsa29I ATCGAT 1 cut(s) 338
Bse1I ACTGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 261
BseCI ATCGAT 1 cut(s) 338
BseMI GCAATG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 175
BseXI GCAGC 2 cut(s) 224, 409
BseYI CCCAGC 1 cut(s) 214
BshVI ATCGAT 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 307
Bsp1286I GDGCHC 2 cut(s) 221, 314
Bsp143I GATC 2 cut(s) 87, 335
BspACI CCGC 1 cut(s) 161
BspDI ATCGAT 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 220, 383
BspMI ACCTGC 1 cut(s) 219
BspPI GGATC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 1 cut(s) 385
BssMI GATC 2 cut(s) 87, 335
Bst4CI ACNGT 2 cut(s) 64, 174
BstKTI GATC 2 cut(s) 90, 338
BstMBI GATC 2 cut(s) 87, 335
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 224, 409
Bsu15I ATCGAT 1 cut(s) 338
BsuTUI ATCGAT 1 cut(s) 338
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 3 cut(s) 69, 392, 440
BveI ACCTGC 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 107
ClaI ATCGAT 1 cut(s) 338
CseI GACGC 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviAII CATG 4 cut(s) 35, 297, 331, 367
DpnI GATC 2 cut(s) 89, 337
DpnII GATC 2 cut(s) 87, 335
Eco24I GRGCYC 1 cut(s) 221
Eco32I GATATC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 307
EcoRV GATATC 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 221
FaeI CATG 4 cut(s) 38, 300, 334, 370
FaqI GGGAC 1 cut(s) 73
FatI CATG 4 cut(s) 34, 296, 330, 366
FauNDI CATATG 1 cut(s) 427
Fnu4HI GCNGC 3 cut(s) 162, 238, 423
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 3 cut(s) 162, 238, 423
FspBI CTAG 1 cut(s) 26
GluI GCNGC 3 cut(s) 162, 238, 423
GsaI CCCAGC 1 cut(s) 218
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 229
Hin1II CATG 4 cut(s) 38, 300, 334, 370
HincII GTYRAC 2 cut(s) 129, 139
HindII GTYRAC 2 cut(s) 129, 139
HindIII AAGCTT 2 cut(s) 50, 410
HinfI GANTC 2 cut(s) 205, 340
HpaII CCGG 1 cut(s) 201
HphI GGTGA 2 cut(s) 162, 200
Hpy166II GTNNAC 2 cut(s) 129, 139
Hpy8I GTNNAC 2 cut(s) 129, 139
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 4 cut(s) 37, 106, 152, 232
HpyCH4III ACNGT 2 cut(s) 64, 174
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 5 cut(s) 4, 38, 254, 366, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 4 cut(s) 38, 300, 334, 370
Kzo9I GATC 2 cut(s) 87, 335
LmnI GCTCC 2 cut(s) 224, 381
LpnPI CCDG 9 cut(s) 24, 109, 123, 143, 200, 214, 224, 257, 398
Lsp1109I GCAGC 2 cut(s) 224, 409
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 206
MalI GATC 2 cut(s) 89, 337
MboI GATC 2 cut(s) 87, 335
MboII GAAGA 2 cut(s) 91, 137
MhlI GDGCHC 2 cut(s) 221, 314
MluCI AATT 1 cut(s) 392
MlyI GAGTC 1 cut(s) 214
MmeI TCCRAC 1 cut(s) 380
MnlI CCTC 4 cut(s) 143, 193, 196, 366
MseI TTAA 1 cut(s) 454
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
MwoI GCNNNNNNNGC 1 cut(s) 44
NciI CCSGG 1 cut(s) 202
NdeI CATATG 1 cut(s) 427
NdeII GATC 2 cut(s) 87, 335
NlaIII CATG 4 cut(s) 38, 300, 334, 370
NlaIV GGNNCC 2 cut(s) 220, 383
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 206
PaeR7I CTCGAG 1 cut(s) 307
PaqCI CACCTGC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 3 cut(s) 163, 239, 424
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
PspFI CCCAGC 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 220, 383
PspPI GGNCC 1 cut(s) 107
PspXI VCTCGAGB 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 422
SaqAI TTAA 1 cut(s) 454
SatI GCNGC 3 cut(s) 162, 238, 423
Sau3AI GATC 2 cut(s) 87, 335
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 2 cut(s) 221, 314
SetI ASST 9 cut(s) 11, 54, 98, 138, 144, 213, 355, 414, 424
SfcI CTRYAG 1 cut(s) 348
Sfr274I CTCGAG 1 cut(s) 307
SinI GGWCC 1 cut(s) 107
SlaI CTCGAG 1 cut(s) 307
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 1 cut(s) 392
SsiI CCGC 1 cut(s) 161
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 200
TaaI ACNGT 2 cut(s) 64, 174
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 148, 308, 338
TaqII GACCGA 1 cut(s) 316
TasI AATT 1 cut(s) 392
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 1 cut(s) 454
Tru9I TTAA 1 cut(s) 454
TscAI CASTG 3 cut(s) 69, 392, 447
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 2 cut(s) 237, 422
Tsp45I GTSAC 1 cut(s) 206
TspRI CASTG 3 cut(s) 69, 392, 447
VpaK11BI GGWCC 1 cut(s) 107
XhoI CTCGAG 1 cut(s) 307
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.