MD11G1289900.v1.1

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000337
Physical Location & Seq
Reverse (-)
40951749 .. 40954040
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1289900.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGGGGCAGAAACTGATGTGTTGTAGTACTAGTGTAGTGCAAGGGTTGAAGCCAGTGATGATGATGCTGGTGGTTCAGGCGTCATATGTAGGGATGAATATATCTTACAAACTGGTAGCAGAGGTTGGAATGAATTTCACCATCCTTGTTGCCTACCGTAACATGTTTTCTGCAGCCCTTATGCTTCCTCTTGCCCTTATATTTGAAAGGAAGAGGCCCAAACTTACCTTAGGTATCCTGTTTCAAGGTTTTATCTCCGGATTATTGGGGGCAACCATGGCACAAAACTTTTACATAGAGAGCTTAGGCTTAACATCGGCAACATATGCCGTAGCCTTCAATAACCTTATTCCAGTCATCACGTTCCTCATAGCATTATGTTTTAGAATGGAGAAGTTAGCCCTTGGGACACACGCGGGTAGGGCAAAAGTTGTGGGTACATTGGTTTGTGTAGGAGGGACAATGATCTTCACTTTCTACAAAGGCCACGAGATTGTCATTTGGTCGACGGACGTAAACCTTTTACGTAAATATTCCCACCAAAGCAGCCAGGTCTTGCACAGAAACACTCGCACTCAGTTGCTGGGCTCCGTCTTAGCACTCTGCAGTAGCATCTCTTTTGCACTGTGGCTGATATTTCAGAAAAAAATGACTAGGGATTACCCTTGCCAGTACTCAAGTACGGCCCTGATGTCTATCATGGCATCAATCCAGTCTACTGCTTTTGCCCTCTGCAAGGAGAGGGACTGGAGTCAGTGGAAGTTGGGCTGGGGCATCAAACTTTTCACGGCAGTGTATTCGGGAGTCATTACTTCGGGATTGGTTCTTACTCTGACCGCATGGTGTGTGCTCATGCGAGGGCCTTTGTTTGTGGCTATTTTCAACCCTCTTGGCCTTCTGCTGGTGGCCTTAGCTGCGACTTTGTTGTTAGATGAGAAGTTACACTTAGGAAGCATATTAGGAGGGGTTTTAATCATCTGTGGGTTATATATGGTTTTGTGGAGTCAAAGAGAGGAACTGAAGACGATGATAGAACAGCCACCATCACTACTTGAAGAGTCCCATTCTATTGATGTAGCAACATTGCCAGTGAGCAGTGATAGTACTTGTGATGAAATCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

375

Amino Acids

41.2

Weight (kDa)

8.96

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 19 - 157 1.1e-10 EamA-like transporter family
EamA PF00892 195 - 333 3e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 506, 716
AccII CGCG 1 cut(s) 416
AccIII TCCGGA 1 cut(s) 257
AciI CCGC 2 cut(s) 416, 837
AcsI RAATTY 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 1040
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 5 cut(s) 28, 439, 674, 682, 1105
AfiI CCNNNNNNNGG 2 cut(s) 736, 901
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 6 cut(s) 49, 206, 245, 340, 883, 1055
AhlI ACTAGT 1 cut(s) 29
AjnI CCWGG 1 cut(s) 549
AleI CACNNNNGTG 1 cut(s) 791
AluBI AGCT 2 cut(s) 303, 914
AluI AGCT 2 cut(s) 303, 914
Alw21I GWGCWC 1 cut(s) 852
AlwNI CAGNNNCTG 2 cut(s) 13, 583
Aor13HI TCCGGA 1 cut(s) 257
AoxI GGCC 6 cut(s) 215, 484, 684, 860, 892, 906
ApeKI GCWGC 3 cut(s) 173, 546, 914
ApoI RAATTY 1 cut(s) 133
AspS9I GGNCC 3 cut(s) 216, 685, 860
AsuHPI GGTGA 1 cut(s) 130
AxyI CCTNAGG 1 cut(s) 229
BaeI ACNNNNGTAYC 2 cut(s) 429, 462
BanII GRGCYC 1 cut(s) 590
BauI CACGAG 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 1028
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 3 cut(s) 185, 558, 901
BccI CCATC 2 cut(s) 149, 1051
BceAI ACGGC 3 cut(s) 314, 699, 804
BcgI CGANNNNNNTGC 2 cut(s) 780, 814
BciT130I CCWGG 1 cut(s) 551
BciVI GTATCC 1 cut(s) 245
BcuI ACTAGT 1 cut(s) 29
BfaI CTAG 2 cut(s) 30, 654
BfmI CTRYAG 2 cut(s) 171, 604
BfuI GTATCC 1 cut(s) 245
BisI GCNGC 3 cut(s) 174, 547, 915
BlsI GCNGC 3 cut(s) 175, 548, 916
BmcAI AGTACT 3 cut(s) 28, 674, 1105
Bme1390I CCNGG 1 cut(s) 551
BmgT120I GGNCC 3 cut(s) 216, 685, 860
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 551
BmsI GCATC 4 cut(s) 54, 621, 713, 783
BoxI GACNNNNGTC 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 1028
BpmI CTGGAG 1 cut(s) 769
Bpu10I CCTNAGC 2 cut(s) 304, 910
BpuEI CTTGAG 1 cut(s) 661
BsaAI YACGTR 1 cut(s) 527
BsaBI GATNNNNATC 1 cut(s) 695
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 276, 403
BsaWI WCCGGW 1 cut(s) 257
Bsc4I CCNNNNNNNGG 2 cut(s) 736, 901
Bse1I ACTGG 7 cut(s) 53, 117, 353, 670, 712, 752, 1088
Bse21I CCTNAGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 1082
Bse8I GATNNNNATC 1 cut(s) 695
BseAI TCCGGA 1 cut(s) 257
BseBI CCWGG 1 cut(s) 551
BseDI CCNNGG 2 cut(s) 276, 403
BseGI GGATG 2 cut(s) 99, 141
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 2 cut(s) 736, 901
BseMI GCAATG 1 cut(s) 1082
BseMII CTCAG 1 cut(s) 590
BseNI ACTGG 7 cut(s) 53, 117, 353, 670, 712, 752, 1088
BseXI GCAGC 3 cut(s) 185, 558, 901
BseYI CCCAGC 2 cut(s) 583, 768
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 6 cut(s) 217, 486, 686, 862, 894, 908
BsiHKAI GWGCWC 1 cut(s) 852
BsiSI CCGG 1 cut(s) 258
BslFI GGGAC 4 cut(s) 421, 472, 758, 1045
BslI CCNNNNNNNGG 2 cut(s) 736, 901
BsmFI GGGAC 4 cut(s) 421, 472, 758, 1045
BsnI GGCC 6 cut(s) 217, 486, 686, 862, 894, 908
Bsp1286I GDGCHC 2 cut(s) 590, 852
Bsp13I TCCGGA 1 cut(s) 257
Bsp143I GATC 1 cut(s) 465
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 2 cut(s) 416, 837
BspANI GGCC 6 cut(s) 217, 486, 686, 862, 894, 908
BspCNI CTCAG 1 cut(s) 589
BspEI TCCGGA 1 cut(s) 257
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 1 cut(s) 589
BspMAI CTGCAG 2 cut(s) 175, 608
BsrDI GCAATG 1 cut(s) 1082
BsrI ACTGG 7 cut(s) 53, 117, 353, 670, 712, 752, 1088
BssECI CCNNGG 2 cut(s) 276, 403
BssMI GATC 1 cut(s) 465
BssNI GRCGYC 1 cut(s) 80
BssSI CACGAG 1 cut(s) 488
BssT1I CCWWGG 2 cut(s) 276, 403
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 551
Bst4CI ACNGT 2 cut(s) 158, 627
Bst6I CTCTTC 2 cut(s) 206, 1050
BstACI GRCGYC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 326
BstBAI YACGTR 1 cut(s) 527
BstDEI CTNAG 6 cut(s) 229, 304, 576, 595, 910, 946
BstDSI CCRYGG 1 cut(s) 276
BstENI CCTNNNNNAGG 1 cut(s) 734
BstF5I GGATG 2 cut(s) 99, 141
BstFNI CGCG 1 cut(s) 416
BstKTI GATC 1 cut(s) 468
BstMBI GATC 1 cut(s) 465
BstMWI GCNNNNNNNGC 4 cut(s) 278, 326, 422, 914
BstNI CCWGG 1 cut(s) 551
BstNSI RCATGY 1 cut(s) 166
BstPAI GACNNNNGTC 1 cut(s) 750
BstSCI CCNGG 1 cut(s) 549
BstSFI CTRYAG 2 cut(s) 171, 604
BstSNI TACGTA 1 cut(s) 527
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 3 cut(s) 185, 558, 901
BstV2I GAAGAC 1 cut(s) 1028
Bsu36I CCTNAGG 1 cut(s) 229
BsuI GTATCC 1 cut(s) 245
BsuRI GGCC 6 cut(s) 217, 486, 686, 862, 894, 908
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 2 cut(s) 99, 141
BtsI GCAGTG 2 cut(s) 798, 1102
BtsIMutI CAGTG 6 cut(s) 60, 623, 761, 798, 1095, 1102
CaiI CAGNNNCTG 2 cut(s) 13, 583
Cfr13I GGNCC 3 cut(s) 216, 685, 860
CseI GACGC 1 cut(s) 69
Csp6I GTAC 5 cut(s) 27, 438, 673, 681, 1104
CspCI CAANNNNNGTGG 2 cut(s) 414, 449
CviAII CATG 5 cut(s) 163, 277, 700, 840, 853
CviQI GTAC 5 cut(s) 27, 438, 673, 681, 1104
DdeI CTNAG 6 cut(s) 229, 304, 576, 595, 910, 946
DpnI GATC 1 cut(s) 467
DpnII GATC 1 cut(s) 465
Eam1104I CTCTTC 2 cut(s) 206, 1050
EarI CTCTTC 2 cut(s) 206, 1050
Eco105I TACGTA 1 cut(s) 527
Eco130I CCWWGG 2 cut(s) 276, 403
Eco24I GRGCYC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 1040
Eco81I CCTNAGG 1 cut(s) 229
EcoNI CCTNNNNNAGG 1 cut(s) 734
EcoO109I RGGNCCY 1 cut(s) 860
EcoRII CCWGG 1 cut(s) 549
EcoT14I CCWWGG 2 cut(s) 276, 403
EcoT38I GRGCYC 1 cut(s) 590
ErhI CCWWGG 2 cut(s) 276, 403
FaeI CATG 5 cut(s) 166, 280, 703, 843, 856
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 4 cut(s) 421, 472, 758, 1045
FatI CATG 5 cut(s) 162, 276, 699, 839, 852
FauI CCCGC 1 cut(s) 409
FauNDI CATATG 2 cut(s) 85, 325
FblI GTMKAC 2 cut(s) 506, 716
Fnu4HI GCNGC 3 cut(s) 174, 547, 915
FokI GGATG 2 cut(s) 106, 128
FriOI GRGCYC 1 cut(s) 590
Fsp4HI GCNGC 3 cut(s) 174, 547, 915
FspBI CTAG 2 cut(s) 30, 654
GluI GCNGC 3 cut(s) 174, 547, 915
GsaI CCCAGC 2 cut(s) 587, 772
GsuI CTGGAG 1 cut(s) 769
HaeIII GGCC 6 cut(s) 217, 486, 686, 862, 894, 908
HapII CCGG 1 cut(s) 258
HgaI GACGC 1 cut(s) 69
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 5 cut(s) 166, 280, 703, 843, 856
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HinfI GANTC 4 cut(s) 751, 804, 1003, 1058
HpaII CCGG 1 cut(s) 258
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 3 cut(s) 507, 517, 717
Hpy188I TCNGA 2 cut(s) 642, 834
Hpy188III TCNNGA 3 cut(s) 258, 801, 816
Hpy8I GTNNAC 3 cut(s) 507, 517, 717
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 2 cut(s) 346, 905
HpyCH4III ACNGT 2 cut(s) 158, 627
HpyCH4IV ACGT 3 cut(s) 362, 513, 526
HpyCH4V TGCA 6 cut(s) 40, 173, 559, 606, 623, 735
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 326, 422, 914
HpyF3I CTNAG 6 cut(s) 229, 304, 576, 595, 910, 946
HpySE526I ACGT 3 cut(s) 362, 513, 526
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 5 cut(s) 166, 280, 703, 843, 856
Kpn2I TCCGGA 1 cut(s) 257
Kzo9I GATC 1 cut(s) 465
LmnI GCTCC 1 cut(s) 593
Lsp1109I GCAGC 3 cut(s) 185, 558, 901
LweI GCATC 4 cut(s) 54, 621, 713, 783
MaeI CTAG 2 cut(s) 30, 654
MaeII ACGT 3 cut(s) 362, 513, 526
MaeIII GTNAC 2 cut(s) 158, 939
MalI GATC 1 cut(s) 467
MboI GATC 1 cut(s) 465
MboII GAAGA 4 cut(s) 223, 460, 1033, 1067
MhlI GDGCHC 2 cut(s) 590, 852
MluCI AATT 2 cut(s) 133, 1120
MlyI GAGTC 4 cut(s) 760, 813, 1012, 1067
MmeI TCCRAC 1 cut(s) 106
MroI TCCGGA 1 cut(s) 257
MseI TTAA 2 cut(s) 311, 971
MslI CAYNNNNRTG 1 cut(s) 791
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 1 cut(s) 551
MvaI CCWGG 1 cut(s) 551
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 4 cut(s) 278, 326, 422, 914
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 2 cut(s) 85, 325
NdeII GATC 1 cut(s) 465
NlaIII CATG 5 cut(s) 166, 280, 703, 843, 856
NlaIV GGNNCC 1 cut(s) 589
NspI RCATGY 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 791
PciI ACATGT 1 cut(s) 162
PkrI GCNGC 3 cut(s) 175, 548, 916
PleI GAGTC 4 cut(s) 759, 812, 1011, 1066
PpsI GAGTC 4 cut(s) 759, 812, 1011, 1066
Ppu21I YACGTR 1 cut(s) 527
PscI ACATGT 1 cut(s) 162
PshAI GACNNNNGTC 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 549
PspFI CCCAGC 2 cut(s) 583, 768
PspGI CCWGG 1 cut(s) 549
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 3 cut(s) 216, 685, 860
PstI CTGCAG 2 cut(s) 175, 608
PstNI CAGNNNCTG 2 cut(s) 13, 583
RsaI GTAC 5 cut(s) 28, 439, 674, 682, 1105
RsaNI GTAC 5 cut(s) 27, 438, 673, 681, 1104
RseI CAYNNNNRTG 1 cut(s) 791
SalI GTCGAC 1 cut(s) 505
SaqAI TTAA 2 cut(s) 311, 971
SatI GCNGC 3 cut(s) 174, 547, 915
Sau3AI GATC 1 cut(s) 465
Sau96I GGNCC 3 cut(s) 216, 685, 860
ScaI AGTACT 3 cut(s) 28, 674, 1105
SchI GAGTC 4 cut(s) 760, 813, 1012, 1067
ScrFI CCNGG 1 cut(s) 551
SduI GDGCHC 2 cut(s) 590, 852
SfaNI GCATC 4 cut(s) 54, 621, 713, 783
SfcI CTRYAG 2 cut(s) 171, 604
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 1 cut(s) 676
SmoI CTYRAG 1 cut(s) 676
SnaBI TACGTA 1 cut(s) 527
SpeI ACTAGT 1 cut(s) 29
Sse9I AATT 2 cut(s) 133, 1120
SsiI CCGC 2 cut(s) 416, 837
SspI AATATT 1 cut(s) 533
SspMI CTAG 2 cut(s) 30, 654
StyD4I CCNGG 1 cut(s) 549
StyI CCWWGG 2 cut(s) 276, 403
TaaI ACNGT 2 cut(s) 158, 627
TaiI ACGT 3 cut(s) 365, 516, 529
TaqI TCGA 1 cut(s) 506
TasI AATT 2 cut(s) 133, 1120
TatI WGTACW 3 cut(s) 26, 672, 1103
Tru1I TTAA 2 cut(s) 311, 971
Tru9I TTAA 2 cut(s) 311, 971
TscAI CASTG 6 cut(s) 60, 630, 761, 798, 1095, 1102
TseI GCWGC 3 cut(s) 173, 546, 914
TspDTI ATGAA 2 cut(s) 110, 146
TspGWI ACGGA 2 cut(s) 524, 580
TspRI CASTG 6 cut(s) 60, 630, 761, 798, 1095, 1102
XagI CCTNNNNNAGG 1 cut(s) 734
XapI RAATTY 1 cut(s) 133
XceI RCATGY 1 cut(s) 166
XmiI GTMKAC 2 cut(s) 506, 716
XspI CTAG 2 cut(s) 30, 654
ZrmI AGTACT 3 cut(s) 28, 674, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.