FvH4_7g27400

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000337
Physical Location & Seq
Forward (+)
20498850 .. 20501104
2255 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27400.t2

Sequence Viewer

Length: 1032 bp
ATGGTGCAGAACCATGAGTCTTCTTCTCCTTTAAAGCCGGTTATTATGATGGTGGTTACCCAGATTTTGAACACTGTGATGAACATAATGTATAAATTTGTTACAGTAACAGGGACGAACTTGAATGTTTTCGTTGCCTACCGACTGATGTTTTCAGCAGCCTTTATGGTTCCGCTGGCTCTAATACTCGAAAGGGCTGCTCTGCCACAAAACTTATATATTCTGAGTCTAGCGCTAACATCACCCACATTCCTTGCGGCCATCAACAATCTCATGCCAGCCACTACCTTCGTCGTCGCTCTATGTTTCCGGCAGGAGAAGTTGACGATGCGAAATTCTGGCATAGCCAAAATGGTTGGGACTGTGGTTGGAATAGGAGGAGCAATGCTTTTCATTTTCTACCGAGGACCTGTATTCACATTTTGGGCCACTCATATTGACCTTCTACAGCAATATTACGGCACTAGAAGCGTCTCATCCTCAAGCCACATGAGCAATCCTGCGTTGGGCCTCCTCGCAGGATTCGGCAGCAGCTTGTGCTTCTCATTGTGGCTCGTAATGCAGGTGAAGATGAGTAAGAGTTACCCCTGTCCATACTCAAGCACGGCTTTGATGGCCATCATGGGATCCATTCAGAGCGTTATCTTTGCACTTTGCGTAGAGAGAGATTGGAATCACTGGAGGATGGGTTGGGATATTAGGTTGTGGACAGCTGCATATTCGGGAATTGTGATTTCTGGAGTGGCAGTGGTTGTGATGTCTTGGTGTGTACAAAAGCGAGGGCCACTTTTTGTGTCAATTTTTTCTCCACTTACCCTGTTGATGGTAGCCTTTGCTAGTTCCTTGTTGTTAGGGGAAAACTTTAGCCTCGGAAGTGTATTAGGAGGAGTTCTAATTGTGTGCGGGCTATACATGGTGCTTTGGGGAAAAAGCAAAGAAGGACCATCGCCGCTTGTGCTACCCTCGTTGACAACAGCTCCCCTAATTTCCAAGTCCGTAGATAGCGCTCCGGTTCAAACGAGTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

344

Amino Acids

37.31

Weight (kDa)

9.46

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 553
Acc36I ACCTGC 1 cut(s) 553
AciI CCGC 4 cut(s) 173, 257, 903, 950
AclWI GGATC 2 cut(s) 621, 634
AcoI YGGCCR 2 cut(s) 258, 615
AcsI RAATTY 2 cut(s) 95, 334
AfaI GTAC 1 cut(s) 771
AfeI AGCGCT 2 cut(s) 234, 1006
AfiI CCNNNNNNNGG 2 cut(s) 506, 823
AgsI TTSAA 3 cut(s) 70, 124, 1016
AluBI AGCT 3 cut(s) 534, 713, 977
AluI AGCT 3 cut(s) 534, 713, 977
Alw26I GTCTC 1 cut(s) 478
AlwI GGATC 2 cut(s) 621, 634
Aor51HI AGCGCT 2 cut(s) 234, 1006
AoxI GGCC 5 cut(s) 258, 426, 508, 615, 782
ApeKI GCWGC 5 cut(s) 158, 197, 528, 531, 713
ApoI RAATTY 2 cut(s) 95, 334
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 2 cut(s) 235, 1007
AspS9I GGNCC 5 cut(s) 407, 426, 508, 782, 941
AsuHPI GGTGA 2 cut(s) 234, 577
AvaII GGWCC 2 cut(s) 407, 941
BalI TGGCCA 1 cut(s) 617
BamHI GGATCC 1 cut(s) 626
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 5 cut(s) 170, 184, 540, 543, 700
BccI CCATC 7 cut(s) 43, 269, 607, 626, 679, 817, 952
BceAI ACGGC 2 cut(s) 475, 621
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BcoDI GTCTC 1 cut(s) 478
BfaI CTAG 3 cut(s) 230, 465, 837
BfmI CTRYAG 1 cut(s) 446
BfoI RGCGCY 2 cut(s) 236, 1008
BfuAI ACCTGC 1 cut(s) 553
BisI GCNGC 7 cut(s) 159, 198, 258, 529, 532, 714, 950
BlsI GCNGC 7 cut(s) 160, 199, 259, 530, 533, 715, 951
Bme18I GGWCC 2 cut(s) 407, 941
BmgT120I GGNCC 5 cut(s) 407, 426, 508, 782, 941
BmiI GGNNCC 2 cut(s) 171, 628
BmsI GCATC 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 2 cut(s) 700, 759
BpuEI CTTGAG 2 cut(s) 466, 583
BsaBI GATNNNNATC 2 cut(s) 617, 672
BsaJI CCNNGG 2 cut(s) 403, 868
BsaWI WCCGGW 1 cut(s) 1009
BsaXI ACNNNNNCTCC 6 cut(s) 673, 703, 732, 762, 961, 991
Bsc4I CCNNNNNNNGG 2 cut(s) 506, 823
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 683
Bse3DI GCAATG 1 cut(s) 390
Bse8I GATNNNNATC 2 cut(s) 617, 672
BseDI CCNNGG 2 cut(s) 403, 868
BseGI GGATG 2 cut(s) 476, 690
BseJI GATNNNNATC 2 cut(s) 617, 672
BseLI CCNNNNNNNGG 2 cut(s) 506, 823
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 683
BseRI GAGGAG 3 cut(s) 393, 503, 900
BseXI GCAGC 5 cut(s) 170, 184, 540, 543, 700
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 5 cut(s) 260, 428, 510, 617, 784
BsiSI CCGG 3 cut(s) 38, 310, 1010
BslFI GGGAC 2 cut(s) 127, 373
BslI CCNNNNNNNGG 2 cut(s) 506, 823
BsmAI GTCTC 1 cut(s) 478
BsmBI CGTCTC 1 cut(s) 478
BsmFI GGGAC 2 cut(s) 127, 373
BsnI GGCC 5 cut(s) 260, 428, 510, 617, 784
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 1 cut(s) 626
BspACI CCGC 4 cut(s) 173, 257, 903, 950
BspANI GGCC 5 cut(s) 260, 428, 510, 617, 784
BspCNI CTCAG 1 cut(s) 216
BspLI GGNNCC 2 cut(s) 171, 628
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 2 cut(s) 621, 634
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 1 cut(s) 683
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 403, 868
BssMI GATC 1 cut(s) 626
Bst4CI ACNGT 3 cut(s) 76, 106, 364
BstAPI GCANNNNNTGC 1 cut(s) 537
BstAUI TGTACA 1 cut(s) 769
BstC8I GCNNGC 3 cut(s) 177, 279, 905
BstDEI CTNAG 1 cut(s) 224
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 2 cut(s) 476, 690
BstH2I RGCGCY 2 cut(s) 236, 1008
BstHHI GCGC 2 cut(s) 235, 1007
BstKTI GATC 1 cut(s) 629
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 1 cut(s) 626
BstMWI GCNNNNNNNGC 6 cut(s) 468, 492, 537, 559, 614, 955
BstPI GGTNACC 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 446
BstV1I GCAGC 5 cut(s) 170, 184, 540, 543, 700
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 626
BstYI RGATCY 1 cut(s) 626
BsuRI GGCC 5 cut(s) 260, 428, 510, 617, 784
BtgZI GCGATG 1 cut(s) 930
BtsCI GGATG 2 cut(s) 476, 690
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 3 cut(s) 72, 676, 753
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 3 cut(s) 177, 279, 905
CfoI GCGC 2 cut(s) 235, 1007
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 5 cut(s) 407, 426, 508, 782, 941
CseI GACGC 1 cut(s) 460
Csp6I GTAC 1 cut(s) 770
CspCI CAANNNNNGTGG 4 cut(s) 235, 270, 418, 453
CviAII CATG 5 cut(s) 14, 274, 490, 622, 913
CviQI GTAC 1 cut(s) 770
DdeI CTNAG 1 cut(s) 224
DpnI GATC 1 cut(s) 628
DpnII GATC 1 cut(s) 626
DraI TTTAAA 1 cut(s) 33
EaeI YGGCCR 2 cut(s) 258, 615
Eco47I GGWCC 2 cut(s) 407, 941
Eco47III AGCGCT 2 cut(s) 234, 1006
Eco91I GGTNACC 1 cut(s) 55
EcoO109I RGGNCCY 1 cut(s) 407
EcoO65I GGTNACC 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 478
FaeI CATG 5 cut(s) 17, 277, 493, 625, 916
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 2 cut(s) 127, 373
FatI CATG 5 cut(s) 13, 273, 489, 621, 912
FauI CCCGC 1 cut(s) 896
Fnu4HI GCNGC 7 cut(s) 159, 198, 258, 529, 532, 714, 950
FokI GGATG 2 cut(s) 463, 697
Fsp4HI GCNGC 7 cut(s) 159, 198, 258, 529, 532, 714, 950
FspBI CTAG 3 cut(s) 230, 465, 837
GlaI GCGC 2 cut(s) 234, 1006
GluI GCNGC 7 cut(s) 159, 198, 258, 529, 532, 714, 950
GsuI CTGGAG 2 cut(s) 700, 759
HaeII RGCGCY 2 cut(s) 236, 1008
HaeIII GGCC 5 cut(s) 260, 428, 510, 617, 784
HapII CCGG 3 cut(s) 38, 310, 1010
HgaI GACGC 1 cut(s) 460
HhaI GCGC 2 cut(s) 235, 1007
Hin1II CATG 5 cut(s) 17, 277, 493, 625, 916
Hin6I GCGC 2 cut(s) 233, 1005
HinP1I GCGC 2 cut(s) 233, 1005
HincII GTYRAC 2 cut(s) 324, 969
HindII GTYRAC 2 cut(s) 324, 969
HinfI GANTC 4 cut(s) 17, 226, 522, 673
HpaII CCGG 3 cut(s) 38, 310, 1010
HphI GGTGA 2 cut(s) 234, 577
Hpy166II GTNNAC 4 cut(s) 324, 708, 770, 969
Hpy188I TCNGA 3 cut(s) 225, 636, 872
Hpy188III TCNNGA 2 cut(s) 723, 738
Hpy8I GTNNAC 4 cut(s) 324, 708, 770, 969
Hpy99I CGWCG 2 cut(s) 296, 299
HpyAV CCTTC 3 cut(s) 298, 452, 932
HpyCH4III ACNGT 3 cut(s) 76, 106, 364
HpyCH4V TGCA 4 cut(s) 7, 562, 650, 716
HpyF10VI GCNNNNNNNGC 6 cut(s) 468, 492, 537, 559, 614, 955
HpyF3I CTNAG 1 cut(s) 224
Hsp92II CATG 5 cut(s) 17, 277, 493, 625, 916
HspAI GCGC 2 cut(s) 233, 1005
Kzo9I GATC 1 cut(s) 626
LmnI GCTCC 3 cut(s) 380, 982, 1012
Lsp1109I GCAGC 5 cut(s) 170, 184, 540, 543, 700
LweI GCATC 1 cut(s) 318
MaeI CTAG 3 cut(s) 230, 465, 837
MaeIII GTNAC 4 cut(s) 55, 100, 106, 581
MalI GATC 1 cut(s) 628
MboI GATC 1 cut(s) 626
MboII GAAGA 3 cut(s) 12, 15, 580
MflI RGATCY 1 cut(s) 626
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 6 cut(s) 95, 334, 726, 798, 894, 984
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 2 cut(s) 26, 235
MmeI TCCRAC 2 cut(s) 349, 1006
Mox20I TGGCCA 1 cut(s) 617
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 617
MseI TTAA 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 2 cut(s) 175, 713
MspI CCGG 3 cut(s) 38, 310, 1010
MwoI GCNNNNNNNGC 6 cut(s) 468, 492, 537, 559, 614, 955
NdeII GATC 1 cut(s) 626
NlaIII CATG 5 cut(s) 17, 277, 493, 625, 916
NlaIV GGNNCC 2 cut(s) 171, 628
PaqCI CACCTGC 1 cut(s) 553
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 2 cut(s) 522, 673
PkrI GCNGC 7 cut(s) 160, 199, 259, 530, 533, 715, 951
PleI GAGTC 2 cut(s) 25, 234
PpsI GAGTC 2 cut(s) 25, 234
PpuMI RGGWCCY 1 cut(s) 407
Psp5II RGGWCCY 1 cut(s) 407
PspEI GGTNACC 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 171, 628
PspPI GGNCC 5 cut(s) 407, 426, 508, 782, 941
PspPPI RGGWCCY 1 cut(s) 407
PsuI RGATCY 1 cut(s) 626
PvuII CAGCTG 1 cut(s) 713
RsaI GTAC 1 cut(s) 771
RsaNI GTAC 1 cut(s) 770
RseI CAYNNNNRTG 1 cut(s) 77
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 7 cut(s) 159, 198, 258, 529, 532, 714, 950
Sau3AI GATC 1 cut(s) 626
Sau96I GGNCC 5 cut(s) 407, 426, 508, 782, 941
SchI GAGTC 2 cut(s) 26, 235
SetI ASST 8 cut(s) 290, 412, 444, 536, 567, 704, 715, 979
SfaNI GCATC 1 cut(s) 318
SfcI CTRYAG 1 cut(s) 446
SinI GGWCC 2 cut(s) 407, 941
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 481, 598
SmoI CTYRAG 2 cut(s) 481, 598
Sse9I AATT 6 cut(s) 95, 334, 726, 798, 894, 984
SsiI CCGC 4 cut(s) 173, 257, 903, 950
SspI AATATT 1 cut(s) 455
SspMI CTAG 3 cut(s) 230, 465, 837
TaaI ACNGT 3 cut(s) 76, 106, 364
TaqI TCGA 1 cut(s) 189
TasI AATT 6 cut(s) 95, 334, 726, 798, 894, 984
TatI WGTACW 1 cut(s) 769
TauI GCSGC 2 cut(s) 260, 952
TfiI GAWTC 2 cut(s) 522, 673
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 3 cut(s) 79, 683, 753
TseI GCWGC 5 cut(s) 158, 197, 528, 531, 713
TspDTI ATGAA 2 cut(s) 95, 382
TspGWI ACGGA 1 cut(s) 985
TspRI CASTG 3 cut(s) 79, 683, 753
VpaK11BI GGWCC 2 cut(s) 407, 941
XapI RAATTY 2 cut(s) 95, 334
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 3 cut(s) 230, 465, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.