FvH4_1g29091

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000776
Physical Location & Seq
Reverse (-)
21593979 .. 21596184
2206 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29091.t1

Sequence Viewer

Length: 501 bp
ATGTTGCCGTCGCTTAAGCCTCACTATGTATATTGTTTCAGCGTGCACTTCCTGATCTCAATCCCTAACACATCGAAAAAAAACCCCCAAATCCCTTCACTCCCGGCCCTCTTCCTCCTTACCGGCCCTCTTCCTCCTTACCCCTCTTCCTCCTCTTCCTCCTTACCGGCCCTCTTCCTCTTTACTGGTCCCCAAATCCCTTCACTCCCCTCTTCCTCCTTACCGTGTTTTTTTATCATTGATTTTAATGATGTTAAAGCCATTAAATGGATCAATAAAAGGGCGGTAAGTGTATTCCGTGAATGGAAGCACGACTTGTCCGAGATTCACAAGCTCGAGGGGAGAGACGTGATTCCGGAAAAGCTTTTGCACAGGCGGGATGAGTGGGAGTGGCTTTGCACGCATTTTGAATCGGACAAGCACAAGACTTTTCATGGACCTTTTGCTTTATTGTCAACTATGATTGATCCCTTTTTGTGTCATAAATTTGTGAATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

19.04

Weight (kDa)

8.7

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 267
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 2 cut(s) 284, 376
AclWI GGATC 2 cut(s) 278, 461
AcsI RAATTY 1 cut(s) 485
AfiI CCNNNNNNNGG 1 cut(s) 267
AflII CTTAAG 1 cut(s) 14
AgsI TTSAA 1 cut(s) 410
AjiI CACGTC 1 cut(s) 349
AluBI AGCT 2 cut(s) 334, 364
AluI AGCT 2 cut(s) 334, 364
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 1 cut(s) 339
Alw44I GTGCAC 1 cut(s) 44
AlwI GGATC 2 cut(s) 278, 461
Ama87I CYCGRG 1 cut(s) 335
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 3 cut(s) 105, 124, 168
ApaLI GTGCAC 1 cut(s) 44
ApoI RAATTY 1 cut(s) 485
AspS9I GGNCC 5 cut(s) 106, 125, 169, 188, 437
AsuC2I CCSGG 1 cut(s) 104
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 2 cut(s) 188, 437
BaeGI GKGCMC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 48
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 1 cut(s) 339
BfrI CTTAAG 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 2 cut(s) 188, 437
BmeT110I CYCGRG 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 5 cut(s) 106, 125, 169, 188, 437
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 104
BsaBI GATNNNNATC 1 cut(s) 59
BsaWI WCCGGW 1 cut(s) 355
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse118I RCCGGY 2 cut(s) 122, 166
Bse1I ACTGG 1 cut(s) 190
Bse8I GATNNNNATC 1 cut(s) 59
BseAI TCCGGA 1 cut(s) 355
BseGI GGATG 1 cut(s) 385
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 1 cut(s) 142
BseSI GKGCMC 1 cut(s) 48
BshFI GGCC 3 cut(s) 107, 126, 170
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 4 cut(s) 104, 123, 167, 356
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 339
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 174
BsnI GGCC 3 cut(s) 107, 126, 170
BsoBI CYCGRG 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 48
Bsp13I TCCGGA 1 cut(s) 355
Bsp143I GATC 3 cut(s) 54, 270, 466
BspACI CCGC 2 cut(s) 284, 376
BspANI GGCC 3 cut(s) 107, 126, 170
BspEI TCCGGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 2 cut(s) 278, 461
BspTI CTTAAG 1 cut(s) 14
BsrFI RCCGGY 2 cut(s) 122, 166
BsrI ACTGG 1 cut(s) 190
BssAI RCCGGY 2 cut(s) 122, 166
BssMI GATC 3 cut(s) 54, 270, 466
Bst4CI ACNGT 1 cut(s) 225
Bst6I CTCTTC 6 cut(s) 116, 135, 151, 160, 179, 217
BstAFI CTTAAG 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 44, 401
BstF5I GGATG 1 cut(s) 385
BstKTI GATC 3 cut(s) 57, 273, 469
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 3 cut(s) 54, 270, 466
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 102
BstSLI GKGCMC 1 cut(s) 48
BsuRI GGCC 3 cut(s) 107, 126, 170
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 1 cut(s) 385
Cac8I GCNNGC 2 cut(s) 44, 401
Cfr10I RCCGGY 2 cut(s) 122, 166
Cfr13I GGNCC 5 cut(s) 106, 125, 169, 188, 437
CviAII CATG 1 cut(s) 434
CviJI RGCY 8 cut(s) 19, 107, 126, 170, 260, 334, 364, 394
CviKI_1 RGCY 8 cut(s) 19, 107, 126, 170, 260, 334, 364, 394
DpnI GATC 3 cut(s) 56, 272, 468
DpnII GATC 3 cut(s) 54, 270, 466
Eam1104I CTCTTC 6 cut(s) 116, 135, 151, 160, 179, 217
EarI CTCTTC 6 cut(s) 116, 135, 151, 160, 179, 217
Eco47I GGWCC 2 cut(s) 188, 437
Eco88I CYCGRG 1 cut(s) 335
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 1 cut(s) 437
FaiI YATR 5 cut(s) 27, 31, 435, 461, 483
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 1 cut(s) 174
FatI CATG 1 cut(s) 433
FauI CCCGC 1 cut(s) 369
FokI GGATG 1 cut(s) 392
HaeIII GGCC 3 cut(s) 107, 126, 170
HapII CCGG 4 cut(s) 104, 123, 167, 356
Hin1II CATG 1 cut(s) 437
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 3 cut(s) 325, 352, 410
HpaII CCGG 4 cut(s) 104, 123, 167, 356
Hpy166II GTNNAC 2 cut(s) 46, 456
Hpy188I TCNGA 2 cut(s) 322, 415
Hpy188III TCNNGA 2 cut(s) 52, 356
Hpy8I GTNNAC 2 cut(s) 46, 456
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 105, 210
HpyCH4III ACNGT 1 cut(s) 225
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 3 cut(s) 46, 370, 399
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 1 cut(s) 437
Kpn2I TCCGGA 1 cut(s) 355
Kzo9I GATC 3 cut(s) 54, 270, 466
LpnPI CCDG 7 cut(s) 65, 117, 136, 171, 180, 358, 369
MaeII ACGT 1 cut(s) 348
MalI GATC 3 cut(s) 56, 272, 468
MboI GATC 3 cut(s) 54, 270, 466
MboII GAAGA 6 cut(s) 103, 122, 138, 147, 166, 204
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 1 cut(s) 485
MroI TCCGGA 1 cut(s) 355
MseI TTAA 4 cut(s) 15, 246, 255, 264
MspCI CTTAAG 1 cut(s) 14
MspI CCGG 4 cut(s) 104, 123, 167, 356
MspR9I CCNGG 1 cut(s) 104
MwoI GCNNNNNNNGC 1 cut(s) 400
NciI CCSGG 1 cut(s) 104
NdeII GATC 3 cut(s) 54, 270, 466
NlaIII CATG 1 cut(s) 437
NlaIV GGNNCC 1 cut(s) 190
PaeR7I CTCGAG 1 cut(s) 335
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PfeI GAWTC 3 cut(s) 325, 352, 410
PflMI CCANNNNNTGG 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 5 cut(s) 106, 125, 169, 188, 437
PspXI VCTCGAGB 1 cut(s) 335
SaqAI TTAA 4 cut(s) 15, 246, 255, 264
Sau3AI GATC 3 cut(s) 54, 270, 466
Sau96I GGNCC 5 cut(s) 106, 125, 169, 188, 437
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 1 cut(s) 48
SetI ASST 4 cut(s) 336, 351, 366, 442
Sfr274I CTCGAG 1 cut(s) 335
SinI GGWCC 2 cut(s) 188, 437
SlaI CTCGAG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 14, 335
SmoI CTYRAG 2 cut(s) 14, 335
Sse9I AATT 1 cut(s) 485
SsiI CCGC 2 cut(s) 284, 376
StyD4I CCNGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 225
TaiI ACGT 1 cut(s) 351
TaqI TCGA 2 cut(s) 74, 336
TasI AATT 1 cut(s) 485
TfiI GAWTC 3 cut(s) 325, 352, 410
Tru1I TTAA 4 cut(s) 15, 246, 255, 264
Tru9I TTAA 4 cut(s) 15, 246, 255, 264
TspDTI ATGAA 1 cut(s) 422
TspGWI ACGGA 1 cut(s) 287
Van91I CCANNNNNTGG 1 cut(s) 267
Vha464I CTTAAG 1 cut(s) 14
VneI GTGCAC 1 cut(s) 44
VpaK11BI GGWCC 2 cut(s) 188, 437
XapI RAATTY 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.