FvH4_2g30344

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000776
Physical Location & Seq
Forward (+)
23531354 .. 23531860
507 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30344.t1

Sequence Viewer

Length: 417 bp
ATGGTGTATAATATTGAAAATAACAAGGTTGTTAGCATGCATTTGAGCACTCAACACCCGGATATCCCACTGGATCAGCTACCTCCTTTGGACTGGCCAACTCAACTAAGTGTTGTGAAAAACGTTTGTGGTATTGGACGAGTAGGCGTCCCAGAGAAGAGCAGAAGGTCAACATCTTCCCTTTCACGCTCTCGACCAAATAATGAGGCGATGGCAGAGATAGTGACTGCTAGAAAGGAGGCGGCTGAGGCACAAGAGAGGACCGAAAAAGCCCAGAATATCGCTGAGCAGTTTGCTGCAGATTATCAAGTGTTGAAGTTGCAAATGGATCAAATCATGGCTCGAATAGGGCTACAACCTATGTGTTCACCTTCGGTCCCAAGCCAGGGGGCCTTGACACTTCTCCGATTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.28

Weight (kDa)

7.8

Isoelectric Point (pI)

61.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 242
AclI AACGTT 1 cut(s) 123
AclWI GGATC 2 cut(s) 81, 336
AcoI YGGCCR 1 cut(s) 95
AcyI GRCGYC 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 385, 386
AgsI TTSAA 2 cut(s) 17, 316
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
Alw21I GWGCWC 1 cut(s) 50
AlwI GGATC 2 cut(s) 81, 336
AoxI GGCC 2 cut(s) 95, 390
ApeKI GCWGC 1 cut(s) 296
AspS9I GGNCC 3 cut(s) 261, 376, 390
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 360
AvaII GGWCC 2 cut(s) 261, 376
BalI TGGCCA 1 cut(s) 97
Bbv12I GWGCWC 1 cut(s) 50
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 283
BccI CCATC 1 cut(s) 205
BciT130I CCWGG 1 cut(s) 386
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 2 cut(s) 243, 297
BlpI GCTNAGC 1 cut(s) 285
BlsI GCNGC 2 cut(s) 244, 298
Bme1390I CCNGG 2 cut(s) 59, 386
Bme18I GGWCC 2 cut(s) 261, 376
BmgT120I GGNCC 3 cut(s) 261, 376, 390
BmiI GGNNCC 2 cut(s) 378, 391
BmrFI CCNGG 2 cut(s) 59, 386
Bpu10I CCTNAGC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 385
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 386
Bse1I ACTGG 2 cut(s) 75, 98
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 385
BseLI CCNNNNNNNGG 2 cut(s) 385, 386
BseMII CTCAG 2 cut(s) 237, 276
BseNI ACTGG 2 cut(s) 75, 98
BseXI GCAGC 1 cut(s) 283
BshFI GGCC 2 cut(s) 97, 392
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 2 cut(s) 134, 362
BslI CCNNNNNNNGG 2 cut(s) 385, 386
BsmFI GGGAC 2 cut(s) 134, 362
BsnI GGCC 2 cut(s) 97, 392
Bsp1286I GDGCHC 1 cut(s) 50
Bsp143I GATC 2 cut(s) 73, 328
Bsp1720I GCTNAGC 1 cut(s) 285
BspACI CCGC 1 cut(s) 242
BspANI GGCC 2 cut(s) 97, 392
BspCNI CTCAG 2 cut(s) 238, 277
BspLI GGNNCC 2 cut(s) 378, 391
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 2 cut(s) 81, 336
BspQI GCTCTTC 1 cut(s) 152
BsrI ACTGG 2 cut(s) 75, 98
BssECI CCNNGG 1 cut(s) 385
BssMI GATC 2 cut(s) 73, 328
BssNI GRCGYC 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 386
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 3 cut(s) 107, 246, 285
BstKTI GATC 2 cut(s) 76, 331
BstMBI GATC 2 cut(s) 73, 328
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 2 cut(s) 57, 384
BstSFI CTRYAG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 283
BsuRI GGCC 2 cut(s) 97, 392
BtgZI GCGATG 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 68
Cac8I GCNNGC 1 cut(s) 38
Cfr13I GGNCC 3 cut(s) 261, 376, 390
CseI GACGC 1 cut(s) 136
CviAII CATG 2 cut(s) 37, 337
CviJI RGCY 8 cut(s) 79, 97, 245, 272, 341, 352, 384, 392
CviKI_1 RGCY 8 cut(s) 79, 97, 245, 272, 341, 352, 384, 392
DdeI CTNAG 3 cut(s) 107, 246, 285
DpnI GATC 2 cut(s) 75, 330
DpnII GATC 2 cut(s) 73, 328
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 261, 376
EcoO109I RGGNCCY 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 384
EcoRV GATATC 1 cut(s) 64
EcoT22I ATGCAT 1 cut(s) 42
FaeI CATG 2 cut(s) 40, 340
FaiI YATR 4 cut(s) 9, 38, 338, 362
FaqI GGGAC 2 cut(s) 134, 362
FatI CATG 2 cut(s) 36, 336
Fnu4HI GCNGC 2 cut(s) 243, 297
Fsp4HI GCNGC 2 cut(s) 243, 297
FspBI CTAG 1 cut(s) 231
GluI GCNGC 2 cut(s) 243, 297
HaeIII GGCC 2 cut(s) 97, 392
HapII CCGG 1 cut(s) 59
HgaI GACGC 1 cut(s) 136
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 2 cut(s) 40, 340
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 360
Hpy166II GTNNAC 2 cut(s) 171, 368
Hpy188I TCNGA 1 cut(s) 407
Hpy188III TCNNGA 1 cut(s) 192
Hpy8I GTNNAC 2 cut(s) 171, 368
HpyAV CCTTC 2 cut(s) 159, 381
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 3 cut(s) 40, 299, 322
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 107, 246, 285
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 2 cut(s) 40, 340
Kzo9I GATC 2 cut(s) 73, 328
LguI GCTCTTC 1 cut(s) 152
LpnPI CCDG 7 cut(s) 56, 72, 79, 165, 287, 371, 398
Lsp1109I GCAGC 1 cut(s) 283
MaeI CTAG 1 cut(s) 231
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 223
MalI GATC 2 cut(s) 75, 330
MboI GATC 2 cut(s) 73, 328
MboII GAAGA 2 cut(s) 168, 169
MhlI GDGCHC 1 cut(s) 50
MlsI TGGCCA 1 cut(s) 97
MluNI TGGCCA 1 cut(s) 97
MnlI CCTC 5 cut(s) 93, 199, 232, 241, 252
Mox20I TGGCCA 1 cut(s) 97
Mph1103I ATGCAT 1 cut(s) 42
MscI TGGCCA 1 cut(s) 97
Msp20I TGGCCA 1 cut(s) 97
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 59, 386
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 1 cut(s) 59
NdeII GATC 2 cut(s) 73, 328
NlaIII CATG 2 cut(s) 40, 340
NlaIV GGNNCC 2 cut(s) 378, 391
NmuCI GTSAC 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 42
NspI RCATGY 1 cut(s) 40
PaeI GCATGC 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 244, 298
Psp1406I AACGTT 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 2 cut(s) 378, 391
PspPI GGNCC 3 cut(s) 261, 376, 390
PstI CTGCAG 1 cut(s) 301
SapI GCTCTTC 1 cut(s) 152
SatI GCNGC 2 cut(s) 243, 297
Sau3AI GATC 2 cut(s) 73, 328
Sau96I GGNCC 3 cut(s) 261, 376, 390
ScrFI CCNGG 2 cut(s) 59, 386
SduI GDGCHC 1 cut(s) 50
SetI ASST 7 cut(s) 30, 81, 85, 126, 170, 361, 373
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 2 cut(s) 261, 376
SphI GCATGC 1 cut(s) 40
SsiI CCGC 1 cut(s) 242
SspI AATATT 1 cut(s) 13
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 57, 384
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 193, 343
TaqII GACCGA 2 cut(s) 278, 364
TauI GCSGC 1 cut(s) 245
TscAI CASTG 1 cut(s) 75
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 296
Tsp45I GTSAC 1 cut(s) 223
TspRI CASTG 1 cut(s) 75
VpaK11BI GGWCC 2 cut(s) 261, 376
XceI RCATGY 1 cut(s) 40
XspI CTAG 1 cut(s) 231
Zsp2I ATGCAT 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.