FvH4_3g23880

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000776
Physical Location & Seq
Reverse (-)
16968461 .. 16972889
4429 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23880.t1

Sequence Viewer

Length: 468 bp
ATGGGTATTGCAAATAGAGATATTGAGAATGTGACTCTTGAACCATATCAAGAAGCCTCGACGTCTAATATCCCCGACACGAAGACTATTCGCTTCAACAACAACAACAACATCCACTTCCAACCCCACGATTGTTATGACGCAGAAAAAGATACTTTAGCTTTCATGAAGGTGGATGCATTTCTTCCTAATAATGTTTATTACCATGGATGCCTGAAATCAGGGAGCAGAGCAAAAGGGTCTTACCTTCGTGGTATTAGACGAGCAGATGTCCCAAAGAAGAGCAGAAGGTCAACATCTTCCCCTTCACGCTCTCGACCGAATAATGAGGCGATGGCAAAAATAGTGGCTGCTAGAAAGGACGCGGTTGAGGCACAAGAGAGGGCTGAAAAAACTCAGAAGATCGCTGAGCAGTCTGCTGCAGATTATCAAGTGTTGAAGTTGCAAATGGATCAAATCATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

156

Amino Acids

17.48

Weight (kDa)

9.25

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 65
AccII CGCG 1 cut(s) 365
AciI CCGC 1 cut(s) 365
AclWI GGATC 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 62
AgsI TTSAA 3 cut(s) 41, 97, 439
AluBI AGCT 1 cut(s) 161
AluI AGCT 1 cut(s) 161
AlwI GGATC 1 cut(s) 459
ApeKI GCWGC 2 cut(s) 350, 419
BbsI GAAGAC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 337, 406
BccI CCATC 1 cut(s) 328
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 420
BisI GCNGC 2 cut(s) 351, 420
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 2 cut(s) 352, 421
BmsI GCATC 2 cut(s) 166, 200
BpiI GAAGAC 1 cut(s) 89
Bpu1102I GCTNAGC 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 3 cut(s) 111, 181, 215
BseMII CTCAG 2 cut(s) 399, 410
BseXI GCAGC 2 cut(s) 337, 406
Bsh1236I CGCG 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 320
BsiEI CGRYCG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 257
Bsp143I GATC 2 cut(s) 402, 451
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 1 cut(s) 365
BspCNI CTCAG 2 cut(s) 400, 409
BspFNI CGCG 1 cut(s) 365
BspHI TCATGA 1 cut(s) 165
BspMAI CTGCAG 1 cut(s) 424
BspPI GGATC 1 cut(s) 459
BspQI GCTCTTC 1 cut(s) 275
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 2 cut(s) 402, 451
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 205
Bst6I CTCTTC 1 cut(s) 275
BstACI GRCGYC 1 cut(s) 62
BstDEI CTNAG 2 cut(s) 396, 408
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 3 cut(s) 111, 181, 215
BstFNI CGCG 1 cut(s) 365
BstKTI GATC 2 cut(s) 405, 454
BstMBI GATC 2 cut(s) 402, 451
BstMCI CGRYCG 1 cut(s) 320
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstSFI CTRYAG 1 cut(s) 420
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 2 cut(s) 337, 406
BstV2I GAAGAC 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 205
BtgZI GCGATG 1 cut(s) 347
BtsCI GGATG 3 cut(s) 111, 181, 215
CciI TCATGA 1 cut(s) 165
CseI GACGC 2 cut(s) 149, 371
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 3 cut(s) 166, 206, 460
CviJI RGCY 5 cut(s) 56, 161, 350, 386, 464
CviKI_1 RGCY 5 cut(s) 56, 161, 350, 386, 464
DdeI CTNAG 2 cut(s) 396, 408
DpnI GATC 2 cut(s) 404, 453
DpnII GATC 2 cut(s) 402, 451
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
Eco130I CCWWGG 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 3 cut(s) 169, 209, 463
FaiI YATR 5 cut(s) 46, 138, 167, 207, 461
FaqI GGGAC 1 cut(s) 257
FatI CATG 3 cut(s) 165, 205, 459
Fnu4HI GCNGC 2 cut(s) 351, 420
FokI GGATG 3 cut(s) 98, 188, 222
Fsp4HI GCNGC 2 cut(s) 351, 420
FspBI CTAG 1 cut(s) 354
GluI GCNGC 2 cut(s) 351, 420
HgaI GACGC 2 cut(s) 149, 371
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 3 cut(s) 169, 209, 463
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 1 cut(s) 399
Hpy188III TCNNGA 4 cut(s) 38, 50, 166, 315
Hpy8I GTNNAC 1 cut(s) 294
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 4 cut(s) 163, 257, 282, 315
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 4 cut(s) 11, 179, 422, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 2 cut(s) 396, 408
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 3 cut(s) 169, 209, 463
Kzo9I GATC 2 cut(s) 402, 451
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 2 cut(s) 207, 227
Lsp1109I GCAGC 2 cut(s) 337, 406
LweI GCATC 2 cut(s) 166, 200
MaeI CTAG 1 cut(s) 354
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 404, 453
MboI GATC 2 cut(s) 402, 451
MboII GAAGA 5 cut(s) 94, 176, 291, 292, 412
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 4 cut(s) 67, 322, 364, 375
Mph1103I ATGCAT 1 cut(s) 181
MslI CAYNNNNRTG 1 cut(s) 170
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 1 cut(s) 371
NcoI CCATGG 1 cut(s) 205
NdeII GATC 2 cut(s) 402, 451
NlaIII CATG 3 cut(s) 169, 209, 463
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 181
PagI TCATGA 1 cut(s) 165
PciSI GCTCTTC 1 cut(s) 275
PkrI GCNGC 2 cut(s) 352, 421
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 424
RseI CAYNNNNRTG 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 275
SatI GCNGC 2 cut(s) 351, 420
Sau3AI GATC 2 cut(s) 402, 451
SchI GAGTC 1 cut(s) 28
SetI ASST 5 cut(s) 65, 163, 174, 249, 293
SfaNI GCATC 2 cut(s) 166, 200
SfcI CTRYAG 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 170
SsiI CCGC 1 cut(s) 365
SspMI CTAG 1 cut(s) 354
StyI CCWWGG 1 cut(s) 205
TaiI ACGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 59, 316
TaqII GACCGA 1 cut(s) 334
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 350, 419
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 2 cut(s) 154, 182
XspI CTAG 1 cut(s) 354
ZraI GACGTC 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.