FvH4_4g27150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000776
Physical Location & Seq
Forward (+)
28282559 .. 28283229
671 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27150.t1

Sequence Viewer

Length: 264 bp
ATGGAGGAGATAATGTCCCCAGGAGTTATTTTTACATCCTGTTTTGATCATGTACTTGGTCTTGTGCAAAGACAATCGGTTGCCAACTCCAGCAACCGTGGTAAGAAGGAGTTGGAGTGCCACTCAGGGAGGAAACCTATCATTTACTGCGTGGCCGAGCTTAGGGATAAGGGTGCTTCATTACCGGTGGTTGACGTGTACTTGGTGACATACGACATCAACCAGCCTAAAGCTGCTGAGCATTATATGAGTATCAGTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

88

Amino Acids

9.67

Weight (kDa)

6.26

Isoelectric Point (pI)

64.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 153
AfaI GTAC 3 cut(s) 54, 200, 259
AfiI CCNNNNNNNGG 1 cut(s) 162
AflIII ACRYGT 1 cut(s) 195
AgeI ACCGGT 1 cut(s) 184
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 1 cut(s) 19
AluBI AGCT 2 cut(s) 160, 233
AluI AGCT 2 cut(s) 160, 233
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 1 cut(s) 233
AsiGI ACCGGT 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 217
BbvI GCAGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 21
BclI TGATCA 1 cut(s) 46
BisI GCNGC 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 21
BmgBI CACGTC 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 21
BplI GAGNNNNNCTC 2 cut(s) 107, 139
BpmI CTGGAG 1 cut(s) 73
Bpu10I CCTNAGC 1 cut(s) 161
Bpu1102I GCTNAGC 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 19, 97
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 3 cut(s) 29, 101, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse118I RCCGGY 1 cut(s) 184
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 2 cut(s) 19, 97
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMII CTCAG 2 cut(s) 138, 228
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 220
BshFI GGCC 1 cut(s) 155
BshTI ACCGGT 1 cut(s) 184
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 1 cut(s) 162
BsnI GGCC 1 cut(s) 155
Bsp143I GATC 1 cut(s) 46
Bsp1720I GCTNAGC 1 cut(s) 237
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 2 cut(s) 137, 229
BsrFI RCCGGY 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 19, 97
BssMI GATC 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 1 cut(s) 98
BstDEI CTNAG 3 cut(s) 124, 161, 237
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 35
BstKTI GATC 1 cut(s) 49
BstMBI GATC 1 cut(s) 46
BstNI CCWGG 1 cut(s) 21
BstSCI CCNGG 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 220
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 97
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 1 cut(s) 35
Cfr10I RCCGGY 1 cut(s) 184
Csp6I GTAC 3 cut(s) 53, 199, 258
CspAI ACCGGT 1 cut(s) 184
CviAII CATG 1 cut(s) 50
CviJI RGCY 4 cut(s) 155, 160, 226, 233
CviKI_1 RGCY 4 cut(s) 155, 160, 226, 233
CviQI GTAC 3 cut(s) 53, 199, 258
DdeI CTNAG 3 cut(s) 124, 161, 237
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 19
FaeI CATG 1 cut(s) 53
FaiI YATR 4 cut(s) 51, 211, 246, 248
FatI CATG 1 cut(s) 49
FbaI TGATCA 1 cut(s) 46
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 234
GluI GCNGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 1 cut(s) 155
HapII CCGG 1 cut(s) 185
Hin1II CATG 1 cut(s) 53
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HpaII CCGG 1 cut(s) 185
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 2 cut(s) 193, 199
Hpy8I GTNNAC 2 cut(s) 193, 199
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 1 cut(s) 67
HpyF3I CTNAG 3 cut(s) 124, 161, 237
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 1 cut(s) 46
LpnPI CCDG 7 cut(s) 6, 33, 52, 103, 111, 198, 236
Lsp1109I GCAGC 1 cut(s) 220
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 205
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 1 cut(s) 123
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 21
NdeII GATC 1 cut(s) 46
NlaIII CATG 1 cut(s) 53
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 1 cut(s) 205
PinAI ACCGGT 1 cut(s) 184
PkrI GCNGC 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 19
PspGI CCWGG 1 cut(s) 19
RsaI GTAC 3 cut(s) 54, 200, 259
RsaNI GTAC 3 cut(s) 53, 199, 258
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 21
SetI ASST 5 cut(s) 139, 162, 198, 235, 263
StyD4I CCNGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 1 cut(s) 198
TatI WGTACW 2 cut(s) 52, 198
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.