FvH4_4g10030

Plant transposase (Ptta/En/Spm family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000776
Physical Location & Seq
Reverse (-)
12517409 .. 12519902
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10030.t1

Sequence Viewer

Length: 702 bp
ATGGGACTGAAATCACTGGAGATGGTGGCCGGAGGAGCGAGTTCCTGCGTCGGGACTCCACCACGCAACCGGAGCTTCCGAGAGCTTTTCCTCTCTCACGGCGGAGCTAGGGCGGTTCCCACCGGTCCAAGAAGAGAGCTGGGTCGACGGCCGACCGTTTGGGACCGGTGCGGCGGTCTATGGTGGCCGGACGGCCGCGGACGACGCTGGGAAAGTTTTCGGCGGGAGCGAGAGCTCGGGGAAGGCTCGGGAGAGAGAAGAAGATCGGCTGCAAACAAGAGTAACCGGGACAAGAAGACATTGTACCACTACACCGGGGCGAGGCCTTTCATCTACAATGCGAAGGAATTGAAGGACAAGGGTGAACACCTCTATTTTATTAAAAGTTACTCACAGGTGTATGACTCAGCTCACAACTTGAAAGCAGCTGAAAATGAAGCTAAACTGATGGATAAGCTTGATGAGATCCGTGACAATGTGAGGAAGGAATACCCTGGCATTCCGGAAGAAGATATTGATCTTCATTTTGACCAACAAACTCAAGCGATTGTGTTTGAAAAAGCATTTAGACATAGAAACATGAATGAGATCTGGGTCATGGGACTAGTAGGCAGTTATGGTTTGAGTCAAGGGCCCGATAGGAGCTGCACTTCACGTTCCCAACGAGCTGCACTTGTTGATATTGCTGTGGAGGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

234

Amino Acids

26.59

Weight (kDa)

9.35

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 145
AccII CGCG 1 cut(s) 198
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 7 cut(s) 102, 113, 171, 174, 196, 198, 223
AclWI GGATC 1 cut(s) 460
AcoI YGGCCR 4 cut(s) 27, 149, 185, 193
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 2 cut(s) 51, 321
AgeI ACCGGT 2 cut(s) 122, 165
AgsI TTSAA 3 cut(s) 352, 421, 557
AhlI ACTAGT 1 cut(s) 604
AjnI CCWGG 1 cut(s) 493
Alw21I GWGCWC 1 cut(s) 237
AlwI GGATC 1 cut(s) 460
Ama87I CYCGRG 2 cut(s) 236, 247
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 6 cut(s) 27, 149, 185, 193, 323, 632
ApaI GGGCCC 1 cut(s) 636
ApeKI GCWGC 4 cut(s) 269, 425, 645, 668
AsiGI ACCGGT 2 cut(s) 122, 165
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 4 cut(s) 125, 163, 632, 633
AsuC2I CCSGG 2 cut(s) 287, 316
AsuHPI GGTGA 1 cut(s) 374
AvaI CYCGRG 2 cut(s) 236, 247
AvaII GGWCC 2 cut(s) 125, 163
BaeGI GKGCMC 1 cut(s) 636
BanII GRGCYC 2 cut(s) 237, 636
BarI GAAGNNNNNNTAC 2 cut(s) 287, 319
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 4 cut(s) 256, 437, 632, 655
BccI CCATC 2 cut(s) 16, 442
BceAI ACGGC 3 cut(s) 115, 164, 208
BciT130I CCWGG 1 cut(s) 495
BcnI CCSGG 2 cut(s) 287, 316
BcuI ACTAGT 1 cut(s) 604
BfaI CTAG 2 cut(s) 108, 605
BglII AGATCT 1 cut(s) 588
BisI GCNGC 6 cut(s) 172, 196, 270, 426, 646, 669
BlsI GCNGC 6 cut(s) 173, 197, 271, 427, 647, 670
Bme1390I CCNGG 3 cut(s) 287, 316, 495
Bme18I GGWCC 2 cut(s) 125, 163
BmeT110I CYCGRG 2 cut(s) 236, 247
BmgT120I GGNCC 4 cut(s) 125, 163, 632, 633
BmiI GGNNCC 3 cut(s) 117, 164, 634
BmrFI CCNGG 3 cut(s) 287, 316, 495
BpiI GAAGAC 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 525
BpuMI CCSGG 2 cut(s) 287, 316
BsaBI GATNNNNATC 1 cut(s) 516
BsaJI CCNNGG 3 cut(s) 196, 315, 493
BsaWI WCCGGW 4 cut(s) 69, 122, 165, 502
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 321
Bse118I RCCGGY 2 cut(s) 122, 165
Bse1I ACTGG 1 cut(s) 21
Bse8I GATNNNNATC 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 3 cut(s) 196, 315, 493
BseJI GATNNNNATC 1 cut(s) 516
BseLI CCNNNNNNNGG 2 cut(s) 51, 321
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 1 cut(s) 48
BseSI GKGCMC 1 cut(s) 636
BseX3I CGGCCG 2 cut(s) 149, 193
BseXI GCAGC 4 cut(s) 256, 437, 632, 655
BseYI CCCAGC 2 cut(s) 139, 207
BsgI GTGCAG 2 cut(s) 631, 654
Bsh1236I CGCG 1 cut(s) 198
Bsh1285I CGRYCG 3 cut(s) 152, 156, 196
BshFI GGCC 6 cut(s) 29, 151, 187, 195, 325, 634
BshTI ACCGGT 2 cut(s) 122, 165
BsiEI CGRYCG 3 cut(s) 152, 156, 196
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 2 cut(s) 236, 247
BsiSI CCGG 8 cut(s) 30, 70, 123, 166, 188, 286, 315, 503
BslFI GGGAC 5 cut(s) 18, 67, 176, 302, 615
BslI CCNNNNNNNGG 2 cut(s) 51, 321
BsmFI GGGAC 5 cut(s) 18, 67, 176, 302, 615
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 6 cut(s) 29, 151, 187, 195, 325, 634
BsoBI CYCGRG 2 cut(s) 236, 247
Bsp120I GGGCCC 1 cut(s) 632
Bsp1286I GDGCHC 2 cut(s) 237, 636
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 4 cut(s) 263, 465, 517, 588
BspACI CCGC 7 cut(s) 102, 113, 171, 174, 196, 198, 223
BspANI GGCC 6 cut(s) 29, 151, 187, 195, 325, 634
BspCNI CTCAG 1 cut(s) 419
BspEI TCCGGA 1 cut(s) 502
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 117, 164, 634
BspPI GGATC 1 cut(s) 460
BsrFI RCCGGY 2 cut(s) 122, 165
BsrI ACTGG 1 cut(s) 21
BssAI RCCGGY 2 cut(s) 122, 165
BssECI CCNNGG 3 cut(s) 196, 315, 493
BssMI GATC 4 cut(s) 263, 465, 517, 588
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 127
BstDEI CTNAG 1 cut(s) 406
BstDSI CCRYGG 1 cut(s) 196
BstFNI CGCG 1 cut(s) 198
BstKTI GATC 4 cut(s) 266, 468, 520, 591
BstMBI GATC 4 cut(s) 263, 465, 517, 588
BstMCI CGRYCG 3 cut(s) 152, 156, 196
BstMWI GCNNNNNNNGC 3 cut(s) 35, 72, 204
BstNI CCWGG 1 cut(s) 495
BstSCI CCNGG 3 cut(s) 285, 314, 493
BstSLI GKGCMC 1 cut(s) 636
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 4 cut(s) 256, 437, 632, 655
BstV2I GAAGAC 1 cut(s) 302
BstX2I RGATCY 2 cut(s) 465, 588
BstYI RGATCY 2 cut(s) 465, 588
BstZI CGGCCG 2 cut(s) 149, 193
BsuRI GGCC 6 cut(s) 29, 151, 187, 195, 325, 634
BtgI CCRYGG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 14
Cfr10I RCCGGY 2 cut(s) 122, 165
Cfr13I GGNCC 4 cut(s) 125, 163, 632, 633
Cfr42I CCGCGG 1 cut(s) 199
CseI GACGC 2 cut(s) 37, 213
Csp6I GTAC 1 cut(s) 304
CspAI ACCGGT 2 cut(s) 122, 165
CviAII CATG 2 cut(s) 580, 598
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 1 cut(s) 406
DpnI GATC 4 cut(s) 265, 467, 519, 590
DpnII GATC 4 cut(s) 263, 465, 517, 588
EaeI YGGCCR 4 cut(s) 27, 149, 185, 193
EagI CGGCCG 2 cut(s) 149, 193
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
EciI GGCGGA 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 235
EclXI CGGCCG 2 cut(s) 149, 193
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 2 cut(s) 237, 636
Eco47I GGWCC 2 cut(s) 125, 163
Eco52I CGGCCG 2 cut(s) 149, 193
Eco53kI GAGCTC 1 cut(s) 235
Eco88I CYCGRG 2 cut(s) 236, 247
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 632
EcoRII CCWGG 1 cut(s) 493
EcoT38I GRGCYC 2 cut(s) 237, 636
FaeI CATG 2 cut(s) 583, 601
FaiI YATR 6 cut(s) 181, 402, 573, 581, 599, 618
FaqI GGGAC 5 cut(s) 18, 67, 176, 302, 615
FatI CATG 2 cut(s) 579, 597
FauI CCCGC 1 cut(s) 216
FblI GTMKAC 1 cut(s) 145
Fnu4HI GCNGC 6 cut(s) 172, 196, 270, 426, 646, 669
FriOI GRGCYC 2 cut(s) 237, 636
Fsp4HI GCNGC 6 cut(s) 172, 196, 270, 426, 646, 669
FspBI CTAG 2 cut(s) 108, 605
GluI GCNGC 6 cut(s) 172, 196, 270, 426, 646, 669
GsaI CCCAGC 2 cut(s) 143, 211
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 6 cut(s) 29, 151, 187, 195, 325, 634
HapII CCGG 8 cut(s) 30, 70, 123, 166, 188, 286, 315, 503
HgaI GACGC 2 cut(s) 37, 213
Hin1II CATG 2 cut(s) 583, 601
HincII GTYRAC 1 cut(s) 146
HindII GTYRAC 1 cut(s) 146
HindIII AAGCTT 2 cut(s) 455, 696
HinfI GANTC 3 cut(s) 55, 404, 625
HpaII CCGG 8 cut(s) 30, 70, 123, 166, 188, 286, 315, 503
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 146, 365
Hpy188I TCNGA 1 cut(s) 80
Hpy188III TCNNGA 3 cut(s) 52, 249, 503
Hpy8I GTNNAC 2 cut(s) 146, 365
Hpy99I CGWCG 3 cut(s) 53, 150, 207
HpyAV CCTTC 4 cut(s) 236, 337, 346, 478
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 655
HpyCH4V TGCA 3 cut(s) 272, 648, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 72, 204
HpyF3I CTNAG 1 cut(s) 406
HpySE526I ACGT 1 cut(s) 655
Hsp92II CATG 2 cut(s) 583, 601
Kpn2I TCCGGA 1 cut(s) 502
KspI CCGCGG 1 cut(s) 199
Kzo9I GATC 4 cut(s) 263, 465, 517, 588
LmnI GCTCC 5 cut(s) 35, 72, 104, 226, 642
Lsp1109I GCAGC 4 cut(s) 256, 437, 632, 655
MaeI CTAG 2 cut(s) 108, 605
MaeII ACGT 1 cut(s) 655
MaeIII GTNAC 3 cut(s) 281, 386, 470
MalI GATC 4 cut(s) 265, 467, 519, 590
MboI GATC 4 cut(s) 263, 465, 517, 588
MboII GAAGA 7 cut(s) 144, 270, 273, 307, 512, 518, 521
MflI RGATCY 2 cut(s) 465, 588
MhlI GDGCHC 2 cut(s) 237, 636
MluCI AATT 1 cut(s) 347
MlyI GAGTC 3 cut(s) 49, 398, 634
MnlI CCTC 6 cut(s) 26, 101, 315, 380, 474, 685
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 1 cut(s) 381
MspA1I CMGCKG 2 cut(s) 198, 428
MspI CCGG 8 cut(s) 30, 70, 123, 166, 188, 286, 315, 503
MspR9I CCNGG 3 cut(s) 287, 316, 495
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 1 cut(s) 495
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 3 cut(s) 35, 72, 204
NciI CCSGG 2 cut(s) 287, 316
NdeII GATC 4 cut(s) 263, 465, 517, 588
NlaIII CATG 2 cut(s) 583, 601
NlaIV GGNNCC 3 cut(s) 117, 164, 634
NmuCI GTSAC 1 cut(s) 470
PceI AGGCCT 1 cut(s) 325
PcsI WCGNNNNNNNCGW 2 cut(s) 226, 661
PctI GAATGC 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 216
PinAI ACCGGT 2 cut(s) 122, 165
PkrI GCNGC 6 cut(s) 173, 197, 271, 427, 647, 670
PleI GAGTC 3 cut(s) 49, 398, 633
PpsI GAGTC 3 cut(s) 49, 398, 633
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 493
PspFI CCCAGC 2 cut(s) 139, 207
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 3 cut(s) 117, 164, 634
PspOMI GGGCCC 1 cut(s) 632
PspPI GGNCC 4 cut(s) 125, 163, 632, 633
PsuI RGATCY 2 cut(s) 465, 588
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
SacI GAGCTC 1 cut(s) 237
SacII CCGCGG 1 cut(s) 199
SalI GTCGAC 1 cut(s) 144
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 6 cut(s) 172, 196, 270, 426, 646, 669
Sau3AI GATC 4 cut(s) 263, 465, 517, 588
Sau96I GGNCC 4 cut(s) 125, 163, 632, 633
SchI GAGTC 3 cut(s) 49, 398, 634
ScrFI CCNGG 3 cut(s) 287, 316, 495
SduI GDGCHC 2 cut(s) 237, 636
Sfr303I CCGCGG 1 cut(s) 199
SgrBI CCGCGG 1 cut(s) 199
SinI GGWCC 2 cut(s) 125, 163
SmlI CTYRAG 1 cut(s) 540
SmoI CTYRAG 1 cut(s) 540
SpeI ACTAGT 1 cut(s) 604
Sse9I AATT 1 cut(s) 347
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 7 cut(s) 102, 113, 171, 174, 196, 198, 223
SspMI CTAG 2 cut(s) 108, 605
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 3 cut(s) 285, 314, 493
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 1 cut(s) 658
TaqI TCGA 1 cut(s) 145
TasI AATT 1 cut(s) 347
TauI GCSGC 2 cut(s) 174, 198
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 4 cut(s) 269, 425, 645, 668
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 4 cut(s) 319, 450, 512, 596
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 1 cut(s) 21
VpaK11BI GGWCC 2 cut(s) 125, 163
XmiI GTMKAC 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 2 cut(s) 108, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.