FvH4_4g16210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000776
Physical Location & Seq
Reverse (-)
20176870 .. 20178126
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16210.t1

Sequence Viewer

Length: 510 bp
ATGTATGACTGGTTAATGAAGGTGGCGAACAAGACATACACTTCTTGGAAGGCTAAGCTCCACAAGTTTTTTAAGCATTACAAAGGGGAAAAGGCTCCGAAAGAGTTTGTGCAAGGAGGAAGGATGGATGAGTGGAATTGGCTTAAGCAACACTTTTTGTCACCTCAGTTTCAAAATGATATAAGACAGAAGATCCTTACAATGCTAAGTGAACAACAACCGGACACTCCAGAGGATAAGCTCCTTCAACAAGTAGACCTCAAATCTCAGCTTGACGCATTTGAGAAGGGGCTTGAGAGAGTTGAAATTGAGGGCAAGGAAAGAGCAACAAGGCGTTCTCGTCCCAATGATATAGTTCTTGAGGAGGTTCGGGAGCACGCCCGACAAGTCCAAGAGGAGGCACGGCATGCTCAAGAGGAGGCACAAGCAGTCCGGGAGCAGACTAGAGTAGCTCAGGAGAAGATCCTTGATTATGAGCGCCGTATACAGATGTTGGAGAAGTATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

20.4

Weight (kDa)

7.91

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 255, 484
AclWI GGATC 2 cut(s) 187, 457
AfiI CCNNNNNNNGG 1 cut(s) 397
AflII CTTAAG 1 cut(s) 143
AgsI TTSAA 3 cut(s) 173, 248, 305
AluBI AGCT 4 cut(s) 58, 241, 271, 452
AluI AGCT 4 cut(s) 58, 241, 271, 452
Alw21I GWGCWC 1 cut(s) 378
AlwI GGATC 2 cut(s) 187, 457
AspLEI GCGC 1 cut(s) 480
AsuC2I CCSGG 1 cut(s) 434
AsuHPI GGTGA 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 378
BccI CCATC 1 cut(s) 118
BceAI ACGGC 2 cut(s) 419, 465
BcnI CCSGG 1 cut(s) 434
BfaI CTAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 481
BfrI CTTAAG 1 cut(s) 143
BlpI GCTNAGC 1 cut(s) 54
Bme1390I CCNGG 1 cut(s) 434
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 434
BpmI CTGGAG 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 453
Bpu1102I GCTNAGC 1 cut(s) 54
BpuEI CTTGAG 3 cut(s) 314, 380, 396
BpuMI CCSGG 1 cut(s) 434
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse1I ACTGG 1 cut(s) 14
BseGI GGATG 2 cut(s) 129, 133
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMII CTCAG 3 cut(s) 179, 281, 467
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 3 cut(s) 377, 410, 431
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 2 cut(s) 221, 433
BslFI GGGAC 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 2 cut(s) 192, 462
Bsp1720I GCTNAGC 1 cut(s) 54
BspCNI CTCAG 3 cut(s) 178, 280, 466
BspLI GGNNCC 1 cut(s) 96
BspPI GGATC 2 cut(s) 187, 457
BspTI CTTAAG 1 cut(s) 143
BsrI ACTGG 1 cut(s) 14
BssMI GATC 2 cut(s) 192, 462
BssNAI GTATAC 1 cut(s) 485
Bst1107I GTATAC 1 cut(s) 485
BstAFI CTTAAG 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 407
BstC8I GCNNGC 2 cut(s) 378, 408
BstDEI CTNAG 5 cut(s) 54, 165, 206, 267, 453
BstF5I GGATG 2 cut(s) 129, 133
BstH2I RGCGCY 1 cut(s) 481
BstHHI GCGC 1 cut(s) 480
BstKTI GATC 2 cut(s) 195, 465
BstMBI GATC 2 cut(s) 192, 462
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 432
BstX2I RGATCY 2 cut(s) 192, 462
BstYI RGATCY 2 cut(s) 192, 462
BstZ17I GTATAC 1 cut(s) 485
BtsCI GGATG 2 cut(s) 129, 133
Cac8I GCNNGC 2 cut(s) 378, 408
CfoI GCGC 1 cut(s) 480
CseI GACGC 1 cut(s) 284
CviAII CATG 1 cut(s) 407
CviJI RGCY 8 cut(s) 53, 58, 95, 142, 241, 271, 292, 452
CviKI_1 RGCY 8 cut(s) 53, 58, 95, 142, 241, 271, 292, 452
DdeI CTNAG 5 cut(s) 54, 165, 206, 267, 453
DpnI GATC 2 cut(s) 194, 464
DpnII GATC 2 cut(s) 192, 462
FaeI CATG 1 cut(s) 410
FaiI YATR 8 cut(s) 6, 37, 182, 353, 408, 474, 485, 504
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 1 cut(s) 327
FatI CATG 1 cut(s) 406
FblI GTMKAC 2 cut(s) 255, 484
FokI GGATG 2 cut(s) 136, 140
FspBI CTAG 1 cut(s) 444
GlaI GCGC 1 cut(s) 479
GsuI CTGGAG 1 cut(s) 213
HaeII RGCGCY 1 cut(s) 481
HapII CCGG 2 cut(s) 221, 433
HgaI GACGC 1 cut(s) 284
HhaI GCGC 1 cut(s) 480
Hin1II CATG 1 cut(s) 410
Hin6I GCGC 1 cut(s) 478
HinP1I GCGC 1 cut(s) 478
HpaII CCGG 2 cut(s) 221, 433
HphI GGTGA 1 cut(s) 153
Hpy166II GTNNAC 3 cut(s) 212, 256, 485
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 5 cut(s) 230, 359, 371, 413, 455
Hpy8I GTNNAC 3 cut(s) 212, 256, 485
HpyAV CCTTC 5 cut(s) 13, 43, 114, 254, 280
HpyCH4V TGCA 1 cut(s) 112
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 5 cut(s) 54, 165, 206, 267, 453
Hsp92II CATG 1 cut(s) 410
HspAI GCGC 1 cut(s) 478
Kzo9I GATC 2 cut(s) 192, 462
LmnI GCTCC 5 cut(s) 63, 100, 246, 373, 436
LpnPI CCDG 4 cut(s) 234, 243, 440, 446
MaeI CTAG 1 cut(s) 444
MaeIII GTNAC 1 cut(s) 159
MalI GATC 2 cut(s) 194, 464
MboI GATC 2 cut(s) 192, 462
MboII GAAGA 2 cut(s) 202, 472
MflI RGATCY 2 cut(s) 192, 462
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 2 cut(s) 136, 306
MmeI TCCRAC 1 cut(s) 474
MseI TTAA 3 cut(s) 14, 72, 144
MspCI CTTAAG 1 cut(s) 143
MspI CCGG 2 cut(s) 221, 433
MspR9I CCNGG 1 cut(s) 434
MwoI GCNNNNNNNGC 1 cut(s) 407
NciI CCSGG 1 cut(s) 434
NdeII GATC 2 cut(s) 192, 462
NlaIII CATG 1 cut(s) 410
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 159
NspI RCATGY 1 cut(s) 410
PaeI GCATGC 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 432
PspN4I GGNNCC 1 cut(s) 96
PsrI GAACNNNNNNTAC 2 cut(s) 20, 52
PsuI RGATCY 2 cut(s) 192, 462
SaqAI TTAA 3 cut(s) 14, 72, 144
Sau3AI GATC 2 cut(s) 192, 462
ScrFI CCNGG 1 cut(s) 434
SduI GDGCHC 1 cut(s) 378
SetI ASST 8 cut(s) 24, 60, 166, 243, 261, 273, 369, 454
SmlI CTYRAG 4 cut(s) 143, 293, 359, 411
SmoI CTYRAG 4 cut(s) 143, 293, 359, 411
SphI GCATGC 1 cut(s) 410
Sse9I AATT 2 cut(s) 136, 306
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 1 cut(s) 432
TasI AATT 2 cut(s) 136, 306
Tru1I TTAA 3 cut(s) 14, 72, 144
Tru9I TTAA 3 cut(s) 14, 72, 144
TseFI GTSAC 1 cut(s) 159
Tsp45I GTSAC 1 cut(s) 159
TspDTI ATGAA 1 cut(s) 32
Vha464I CTTAAG 1 cut(s) 143
XceI RCATGY 1 cut(s) 410
XmiI GTMKAC 2 cut(s) 255, 484
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.