FvH4_2g30380

Plant transposase (Ptta/En/Spm family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000776
Physical Location & Seq
Forward (+)
23556582 .. 23561665
5084 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30380.t1

Sequence Viewer

Length: 765 bp
ATGTGGGCGTGGCTTGAAGATAAGGCGGGTACCAAATATCGAGATTGGAAGGGGGACTTTTCAAGGCACTATAAGAAGTATGGGAGACAAACAATTCCGCCTGATTTGATACACAGGCAAGATGAGTGGGACTGGCTTTGCTGGCATTTTAAGTCGCCTGATTTCAAGACACGATCGGCGGCTAACAAGACCAACCGTGGTAAGAAGGAGTCGGAGCACCACACGGGGCGGCTACCTATCATCTACAGTGTGGTCCACCACAAAGAGAAGGGTAAGGAATTTCATGTTATCCCAGCTTACATAGAGACGTATGACACAGTCAACGACCCTAAAGCTGCCAAGAATCTTGAGGAAATGCAGAAAGAGACAACTGAATTGAAAAATGCACAGAAGAATGCAAACCCTGACATCCCGGAGGCTGAGCTCCCACCTTTGGAGTGGTCAATGAATCTTAAGGTACTTGAAGCAGGAGTCGGCAGAAGAGCCGGAAAAGAGATCCATGGTCTTGGAAGAGCAGGTGTCCGACAGTTATCATCTTCCACTCCACGTTCTCGAGTTCATGAGGTGGAGGAGAGATTACATATTGCTGAGGAGAGGGCCGAAAAAGTTGAGGCGGTTGCGCAAAAAGTTAGTGAAGAGATGACATTGGTGAAGCAGCAACTAGCAGTAGTGATGTCTTTTATGGCGCAGCAGACTGGGTGTCCTCCACTAGTCCCTTCCCAACAACCTCTAGAAGAAAATGTTGATGCAGCTGAGAGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

255

Amino Acids

29.21

Weight (kDa)

7.2

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 40 - 132 6e-07 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 506
Acc16I TGCGCA 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 506
Acc65I GGTACC 1 cut(s) 29
AccB1I GGYRCC 1 cut(s) 29
AciI CCGC 5 cut(s) 26, 98, 179, 229, 614
AclWI GGATC 1 cut(s) 490
AcsI RAATTY 1 cut(s) 278
AfaI GTAC 2 cut(s) 31, 459
AfiI CCNNNNNNNGG 1 cut(s) 433
AflII CTTAAG 1 cut(s) 452
AgsI TTSAA 5 cut(s) 17, 63, 166, 379, 464
AhdI GACNNNNNGTC 1 cut(s) 699
AhlI ACTAGT 1 cut(s) 709
AluBI AGCT 4 cut(s) 296, 335, 424, 752
AluI AGCT 4 cut(s) 296, 335, 424, 752
Alw21I GWGCWC 2 cut(s) 219, 426
Alw26I GTCTC 3 cut(s) 79, 299, 359
AlwI GGATC 1 cut(s) 490
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 1 cut(s) 597
ApeKI GCWGC 4 cut(s) 335, 655, 688, 749
ApoI RAATTY 1 cut(s) 278
Asp718I GGTACC 1 cut(s) 29
AspLEI GCGC 2 cut(s) 622, 688
AspS9I GGNCC 2 cut(s) 253, 597
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 552
AvaII GGWCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 426
Bbv12I GWGCWC 2 cut(s) 219, 426
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 322, 667, 700, 761
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 3 cut(s) 79, 299, 359
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 4 cut(s) 662, 710, 731, 763
BfmI CTRYAG 1 cut(s) 244
BfrI CTTAAG 1 cut(s) 452
BfuAI ACCTGC 1 cut(s) 506
BisI GCNGC 6 cut(s) 180, 230, 336, 656, 689, 750
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 6 cut(s) 181, 231, 337, 657, 690, 751
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 1 cut(s) 253
BmeRI GACNNNNNGTC 1 cut(s) 699
BmeT110I CYCGRG 1 cut(s) 552
BmgT120I GGNCC 2 cut(s) 253, 597
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 413
BmrI ACTGGG 1 cut(s) 705
BmsI GCATC 1 cut(s) 736
BmuI ACTGGG 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 588
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 196, 499
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 2 cut(s) 137, 700
BseDI CCNNGG 2 cut(s) 196, 499
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMII CTCAG 3 cut(s) 411, 579, 744
BseNI ACTGG 2 cut(s) 137, 700
BseRI GAGGAG 2 cut(s) 584, 605
BseXI GCAGC 4 cut(s) 322, 667, 700, 761
BseYI CCCAGC 1 cut(s) 292
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 1 cut(s) 599
BshNI GGYRCC 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 176
BsiHKAI GWGCWC 2 cut(s) 219, 426
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 2 cut(s) 413, 486
BslFI GGGAC 3 cut(s) 68, 143, 698
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 3 cut(s) 79, 299, 359
BsmBI CGTCTC 1 cut(s) 299
BsmFI GGGAC 3 cut(s) 68, 143, 698
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 1 cut(s) 599
BsoBI CYCGRG 1 cut(s) 552
Bsp1286I GDGCHC 2 cut(s) 219, 426
Bsp143I GATC 2 cut(s) 173, 495
Bsp1720I GCTNAGC 1 cut(s) 420
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 5 cut(s) 26, 98, 179, 229, 614
BspANI GGCC 1 cut(s) 599
BspCNI CTCAG 3 cut(s) 412, 580, 745
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 31
BspMI ACCTGC 1 cut(s) 506
BspPI GGATC 1 cut(s) 490
BspQI GCTCTTC 2 cut(s) 475, 505
BspT107I GGYRCC 1 cut(s) 29
BspTI CTTAAG 1 cut(s) 452
BsrI ACTGG 2 cut(s) 137, 700
BssECI CCNNGG 2 cut(s) 196, 499
BssMI GATC 2 cut(s) 173, 495
BssT1I CCWWGG 1 cut(s) 499
Bst4CI ACNGT 4 cut(s) 197, 248, 319, 528
Bst6I CTCTTC 3 cut(s) 475, 505, 630
BstAFI CTTAAG 1 cut(s) 452
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 3 cut(s) 420, 588, 753
BstDSI CCRYGG 2 cut(s) 196, 499
BstF5I GGATG 1 cut(s) 408
BstHHI GCGC 2 cut(s) 622, 688
BstKTI GATC 2 cut(s) 176, 498
BstMAI GTCTC 3 cut(s) 79, 299, 359
BstMBI GATC 2 cut(s) 173, 495
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 411
BstSFI CTRYAG 1 cut(s) 244
BstV1I GCAGC 4 cut(s) 322, 667, 700, 761
BstX2I RGATCY 1 cut(s) 495
BstXI CCANNNNNNTGG 1 cut(s) 506
BstYI RGATCY 1 cut(s) 495
BsuRI GGCC 1 cut(s) 599
BtgI CCRYGG 2 cut(s) 196, 499
BtsCI GGATG 1 cut(s) 408
BtsIMutI CAGTG 1 cut(s) 253
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 1 cut(s) 143
CciI TCATGA 1 cut(s) 559
CfoI GCGC 2 cut(s) 622, 688
Cfr13I GGNCC 2 cut(s) 253, 597
Csp6I GTAC 2 cut(s) 30, 458
CspCI CAANNNNNGTGG 2 cut(s) 107, 142
CviAII CATG 3 cut(s) 284, 500, 560
CviQI GTAC 2 cut(s) 30, 458
DdeI CTNAG 3 cut(s) 420, 588, 753
DpnI GATC 2 cut(s) 175, 497
DpnII GATC 2 cut(s) 173, 495
DriI GACNNNNNGTC 1 cut(s) 699
Eam1104I CTCTTC 3 cut(s) 475, 505, 630
Eam1105I GACNNNNNGTC 1 cut(s) 699
EarI CTCTTC 3 cut(s) 475, 505, 630
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 424
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 426
Eco47I GGWCC 1 cut(s) 253
Eco53kI GAGCTC 1 cut(s) 424
Eco88I CYCGRG 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 499
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 3 cut(s) 287, 503, 563
FaiI YATR 9 cut(s) 72, 81, 285, 302, 312, 501, 561, 582, 683
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 3 cut(s) 68, 143, 698
FatI CATG 3 cut(s) 283, 499, 559
FauI CCCGC 1 cut(s) 19
Fnu4HI GCNGC 6 cut(s) 180, 230, 336, 656, 689, 750
FokI GGATG 1 cut(s) 395
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 6 cut(s) 180, 230, 336, 656, 689, 750
FspBI CTAG 4 cut(s) 662, 710, 731, 763
FspI TGCGCA 1 cut(s) 621
GlaI GCGC 2 cut(s) 621, 687
GluI GCNGC 6 cut(s) 180, 230, 336, 656, 689, 750
GsaI CCCAGC 1 cut(s) 296
HaeIII GGCC 1 cut(s) 599
HapII CCGG 2 cut(s) 413, 486
HhaI GCGC 2 cut(s) 622, 688
Hin1II CATG 3 cut(s) 287, 503, 563
Hin6I GCGC 2 cut(s) 620, 686
HinP1I GCGC 2 cut(s) 620, 686
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 4 cut(s) 209, 343, 448, 471
HpaII CCGG 2 cut(s) 413, 486
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 2 cut(s) 256, 322
Hpy188I TCNGA 2 cut(s) 214, 524
Hpy188III TCNNGA 6 cut(s) 41, 166, 347, 552, 560, 731
Hpy8I GTNNAC 2 cut(s) 256, 322
HpyAV CCTTC 4 cut(s) 43, 199, 262, 726
HpyCH4III ACNGT 4 cut(s) 197, 248, 319, 528
HpyCH4IV ACGT 2 cut(s) 308, 547
HpyCH4V TGCA 4 cut(s) 358, 386, 398, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 142
HpyF3I CTNAG 3 cut(s) 420, 588, 753
HpySE526I ACGT 2 cut(s) 308, 547
Hsp92II CATG 3 cut(s) 287, 503, 563
HspAI GCGC 2 cut(s) 620, 686
KpnI GGTACC 1 cut(s) 33
Kzo9I GATC 2 cut(s) 173, 495
LguI GCTCTTC 2 cut(s) 475, 505
LmnI GCTCC 2 cut(s) 214, 429
Lsp1109I GCAGC 4 cut(s) 322, 667, 700, 761
LweI GCATC 1 cut(s) 736
MaeI CTAG 4 cut(s) 662, 710, 731, 763
MaeII ACGT 2 cut(s) 308, 547
MalI GATC 2 cut(s) 175, 497
MboI GATC 2 cut(s) 173, 495
MboII GAAGA 7 cut(s) 29, 403, 492, 522, 528, 647, 746
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 2 cut(s) 219, 426
MluCI AATT 3 cut(s) 93, 278, 374
MlyI GAGTC 2 cut(s) 218, 480
MmeI TCCRAC 2 cut(s) 192, 547
MseI TTAA 2 cut(s) 150, 453
MspA1I CMGCKG 1 cut(s) 752
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 2 cut(s) 413, 486
MspR9I CCNGG 1 cut(s) 413
Mva1269I GAATGC 1 cut(s) 400
MwoI GCNNNNNNNGC 1 cut(s) 142
NciI CCSGG 1 cut(s) 413
NcoI CCATGG 1 cut(s) 499
NdeII GATC 2 cut(s) 173, 495
NlaIII CATG 3 cut(s) 287, 503, 563
NlaIV GGNNCC 1 cut(s) 31
NsbI TGCGCA 1 cut(s) 621
PaeR7I CTCGAG 1 cut(s) 552
PagI TCATGA 1 cut(s) 559
PaqCI CACCTGC 1 cut(s) 506
PciSI GCTCTTC 2 cut(s) 475, 505
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 2 cut(s) 343, 448
PflFI GACNNNGTC 1 cut(s) 317
PfoI TCCNGGA 1 cut(s) 411
PkrI GCNGC 6 cut(s) 181, 231, 337, 657, 690, 751
Ple19I CGATCG 1 cut(s) 176
PleI GAGTC 2 cut(s) 217, 479
PpsI GAGTC 2 cut(s) 217, 479
Psp124BI GAGCTC 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 292
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 2 cut(s) 253, 597
PsuI RGATCY 1 cut(s) 495
PsyI GACNNNGTC 1 cut(s) 317
PvuI CGATCG 1 cut(s) 176
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 2 cut(s) 31, 459
RsaNI GTAC 2 cut(s) 30, 458
SacI GAGCTC 1 cut(s) 426
SapI GCTCTTC 2 cut(s) 475, 505
SaqAI TTAA 2 cut(s) 150, 453
SatI GCNGC 6 cut(s) 180, 230, 336, 656, 689, 750
Sau3AI GATC 2 cut(s) 173, 495
Sau96I GGNCC 2 cut(s) 253, 597
SchI GAGTC 2 cut(s) 218, 480
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 2 cut(s) 219, 426
SfaNI GCATC 1 cut(s) 736
SfcI CTRYAG 1 cut(s) 244
Sfr274I CTCGAG 1 cut(s) 552
SinI GGWCC 1 cut(s) 253
SlaI CTCGAG 1 cut(s) 552
SmlI CTYRAG 3 cut(s) 347, 452, 552
SmoI CTYRAG 3 cut(s) 347, 452, 552
SpeI ACTAGT 1 cut(s) 709
Sse9I AATT 3 cut(s) 93, 278, 374
SsiI CCGC 5 cut(s) 26, 98, 179, 229, 614
SspMI CTAG 4 cut(s) 662, 710, 731, 763
SstI GAGCTC 1 cut(s) 426
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 4 cut(s) 197, 248, 319, 528
TaiI ACGT 2 cut(s) 311, 550
TaqI TCGA 2 cut(s) 40, 553
TasI AATT 3 cut(s) 93, 278, 374
TauI GCSGC 2 cut(s) 182, 232
TfiI GAWTC 2 cut(s) 343, 448
Tru1I TTAA 2 cut(s) 150, 453
Tru9I TTAA 2 cut(s) 150, 453
TscAI CASTG 1 cut(s) 253
TseI GCWGC 4 cut(s) 335, 655, 688, 749
TspDTI ATGAA 3 cut(s) 272, 461, 548
TspRI CASTG 1 cut(s) 253
Tth111I GACNNNGTC 1 cut(s) 317
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 1 cut(s) 253
XapI RAATTY 1 cut(s) 278
XbaI TCTAGA 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 435
XhoI CTCGAG 1 cut(s) 552
XspI CTAG 4 cut(s) 662, 710, 731, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.