FvH4_3g32572

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000776
Physical Location & Seq
Forward (+)
27898211 .. 27901547
3337 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32572.t1

Sequence Viewer

Length: 465 bp
ATGTGGGCGTGGCTTGAAGATAAGGCGGGTACCATATATAGAGATTGGAAGGGGGACTTTTCAAGGCACTATAAGAAGTATGGGAGAGGAACAATCCCGCCAGATTTGATACACAGGCGGGATGAGTGGGACTGGCTTTGCTGGCACTTTGAGTCGGCTGATTTCAAGAAAGAAGCCTCTAAATTGAAAAATGAGCAGAAGAATGCAAACCCTGATGTCCCAGAGGGTGAGCTCCCACCTTTGGAGTGGTCGATAAACATTAAGGTGCTTGAAGCAGGAGTCGGTAGAAGAGCCGGGAAGGAGATTCGTGGTCTTGGAAGAGCAGGTGTACGAGAGTCATCATCTTCCGCTCCACGTTCTCGAGTTTATGAGGTGGAGGACAGATTACATATTGCTGAGGAGAGGGCCGAAAAAGCCGAGGTGGTTGTGCAGAACCTTAGTCAAGAGATGGCATTGGTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

155

Amino Acids

17.83

Weight (kDa)

6.93

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 314
Acc36I ACCTGC 1 cut(s) 314
Acc65I GGTACC 1 cut(s) 29
AccB1I GGYRCC 1 cut(s) 29
AccBSI CCGCTC 1 cut(s) 350
AciI CCGC 4 cut(s) 26, 98, 118, 348
AfaI GTAC 2 cut(s) 31, 330
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 5 cut(s) 17, 63, 166, 187, 272
AluBI AGCT 1 cut(s) 232
AluI AGCT 1 cut(s) 232
Alw21I GWGCWC 1 cut(s) 234
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 1 cut(s) 405
Asp718I GGTACC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 1 cut(s) 239
AvaI CYCGRG 1 cut(s) 360
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 234
BbvCI CCTCAGC 1 cut(s) 396
BccI CCATC 1 cut(s) 442
BcnI CCSGG 1 cut(s) 295
BfuAI ACCTGC 1 cut(s) 314
Bme1390I CCNGG 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 295
Bpu10I CCTNAGC 1 cut(s) 396
BpuMI CCSGG 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 417
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse1I ACTGG 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 1 cut(s) 413
BsgI GTGCAG 1 cut(s) 449
BshFI GGCC 1 cut(s) 407
BshNI GGYRCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 3 cut(s) 68, 143, 203
BslI CCNNNNNNNGG 1 cut(s) 241
BsmFI GGGAC 3 cut(s) 68, 143, 203
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 1 cut(s) 407
BsoBI CYCGRG 1 cut(s) 360
Bsp1286I GDGCHC 1 cut(s) 234
BspACI CCGC 4 cut(s) 26, 98, 118, 348
BspANI GGCC 1 cut(s) 407
BspCNI CTCAG 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 31
BspMI ACCTGC 1 cut(s) 314
BspQI GCTCTTC 2 cut(s) 283, 313
BspT107I GGYRCC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 350
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 417
Bst6I CTCTTC 2 cut(s) 283, 313
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 2 cut(s) 396, 437
BstF5I GGATG 1 cut(s) 127
BstMWI GCNNNNNNNGC 2 cut(s) 142, 413
BstSCI CCNGG 1 cut(s) 293
BsuRI GGCC 1 cut(s) 407
BtsCI GGATG 1 cut(s) 127
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 405
Csp6I GTAC 2 cut(s) 30, 329
CviJI RGCY 8 cut(s) 13, 136, 158, 176, 232, 293, 407, 416
CviKI_1 RGCY 8 cut(s) 13, 136, 158, 176, 232, 293, 407, 416
CviQI GTAC 2 cut(s) 30, 329
DdeI CTNAG 2 cut(s) 396, 437
Eam1104I CTCTTC 2 cut(s) 283, 313
EarI CTCTTC 2 cut(s) 283, 313
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 234
FaiI YATR 7 cut(s) 35, 37, 39, 72, 81, 369, 390
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 3 cut(s) 68, 143, 203
FauI CCCGC 3 cut(s) 19, 105, 111
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 234
HaeIII GGCC 1 cut(s) 407
HapII CCGG 1 cut(s) 294
HinfI GANTC 4 cut(s) 152, 279, 304, 335
HpaII CCGG 1 cut(s) 294
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 1 cut(s) 329
Hpy188III TCNNGA 3 cut(s) 166, 360, 443
Hpy8I GTNNAC 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 43, 292
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 2 cut(s) 206, 430
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 413
HpyF3I CTNAG 2 cut(s) 396, 437
HpySE526I ACGT 1 cut(s) 355
KpnI GGTACC 1 cut(s) 33
LguI GCTCTTC 2 cut(s) 283, 313
LmnI GCTCC 2 cut(s) 237, 355
LpnPI CCDG 9 cut(s) 100, 114, 118, 127, 225, 234, 261, 307, 309
MaeII ACGT 1 cut(s) 355
MbiI CCGCTC 1 cut(s) 350
MboII GAAGA 5 cut(s) 29, 211, 300, 330, 336
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 1 cut(s) 182
MlyI GAGTC 3 cut(s) 161, 288, 344
MnlI CCTC 8 cut(s) 80, 187, 217, 364, 370, 391, 396, 412
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 142, 413
NciI CCSGG 1 cut(s) 295
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 442
PaeR7I CTCGAG 1 cut(s) 360
PaqCI CACCTGC 1 cut(s) 314
PciSI GCTCTTC 2 cut(s) 283, 313
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 304
PleI GAGTC 3 cut(s) 160, 287, 343
PpsI GAGTC 3 cut(s) 160, 287, 343
Psp124BI GAGCTC 1 cut(s) 234
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 405
RsaI GTAC 2 cut(s) 31, 330
RsaNI GTAC 2 cut(s) 30, 329
RseI CAYNNNNRTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 234
SapI GCTCTTC 2 cut(s) 283, 313
SaqAI TTAA 1 cut(s) 261
Sau96I GGNCC 1 cut(s) 405
SchI GAGTC 3 cut(s) 161, 288, 344
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 1 cut(s) 234
SetI ASST 8 cut(s) 234, 241, 267, 328, 358, 375, 423, 438
Sfr274I CTCGAG 1 cut(s) 360
SlaI CTCGAG 1 cut(s) 360
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
Sse9I AATT 1 cut(s) 182
SsiI CCGC 4 cut(s) 26, 98, 118, 348
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 293
TaiI ACGT 1 cut(s) 358
TaqI TCGA 2 cut(s) 251, 361
TasI AATT 1 cut(s) 182
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
XcmI CCANNNNNNNNNTGG 1 cut(s) 243
XhoI CTCGAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.