FvH4_3g31911

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000776
Physical Location & Seq
Reverse (-)
26967932 .. 26968240
309 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31911.t1

Sequence Viewer

Length: 309 bp
ATGAATGAGATCCGAGGCATGGCACTAGTAGGTAGTTATGGTTTGAGTCAAGGGCCCGATAGGAGCTGCACTTCACGTTCCCAACGAGCTACACTTATTGATATTACTGTGGAGGAAGCCCGAAAGGCCAAGGAGGAAGCCCGAAAAGCCAAGGAAGAGAACATACAAATCATGGCACGTCTTCAACAATGTGAGCAGTTTCTCTTCACCAAATTCGGCGTATGCCAGCCGGCAGTCCCACCACAGGAGCAGCCCAATGATGATGATCATGATGATGGAGGCATTGATTCAATTGACTTTGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

11.36

Weight (kDa)

4.69

Isoelectric Point (pI)

66.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 212
AfiI CCNNNNNNNGG 2 cut(s) 19, 244
AgsI TTSAA 2 cut(s) 185, 291
AhlI ACTAGT 1 cut(s) 25
AjiI CACGTC 1 cut(s) 179
AluBI AGCT 2 cut(s) 66, 89
AluI AGCT 2 cut(s) 66, 89
AlwI GGATC 1 cut(s) 4
AoxI GGCC 2 cut(s) 53, 126
ApaI GGGCCC 1 cut(s) 57
ApeKI GCWGC 2 cut(s) 66, 250
ApoI RAATTY 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 53, 54
AsuHPI GGTGA 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 57
BanII GRGCYC 1 cut(s) 57
BarI GAAGNNNNNNTAC 2 cut(s) 147, 179
BbsI GAAGAC 1 cut(s) 173
BbvI GCAGC 2 cut(s) 53, 262
BccI CCATC 1 cut(s) 269
BclI TGATCA 1 cut(s) 265
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 1 cut(s) 26
BglI GCCNNNNNGGC 1 cut(s) 125
BisI GCNGC 2 cut(s) 67, 251
BlsI GCNGC 2 cut(s) 68, 252
BmgBI CACGTC 1 cut(s) 179
BmgT120I GGNCC 2 cut(s) 53, 54
BmiI GGNNCC 1 cut(s) 55
BpiI GAAGAC 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 264
BsaJI CCNNGG 3 cut(s) 13, 129, 150
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 244
Bse118I RCCGGY 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 13, 129, 150
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 2 cut(s) 19, 244
BseSI GKGCMC 1 cut(s) 57
BseXI GCAGC 2 cut(s) 53, 262
BsgI GTGCAG 1 cut(s) 52
BshFI GGCC 2 cut(s) 55, 128
BsiSI CCGG 1 cut(s) 230
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 19, 244
BsmFI GGGAC 1 cut(s) 221
BsnI GGCC 2 cut(s) 55, 128
Bsp120I GGGCCC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 9, 265
BspANI GGCC 2 cut(s) 55, 128
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 3 cut(s) 13, 129, 150
BssMI GATC 2 cut(s) 9, 265
BssT1I CCWWGG 2 cut(s) 129, 150
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 2 cut(s) 150, 209
BstC8I GCNNGC 2 cut(s) 227, 231
BstKTI GATC 2 cut(s) 12, 268
BstMBI GATC 2 cut(s) 9, 265
BstMWI GCNNNNNNNGC 2 cut(s) 125, 146
BstSLI GKGCMC 1 cut(s) 57
BstV1I GCAGC 2 cut(s) 53, 262
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 2 cut(s) 55, 128
BtrI CACGTC 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 227, 231
CciI TCATGA 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 2 cut(s) 53, 54
CviAII CATG 3 cut(s) 19, 172, 269
CviJI RGCY 9 cut(s) 55, 66, 89, 119, 128, 140, 149, 229, 253
CviKI_1 RGCY 9 cut(s) 55, 66, 89, 119, 128, 140, 149, 229, 253
DpnI GATC 2 cut(s) 11, 267
DpnII GATC 2 cut(s) 9, 265
Eam1104I CTCTTC 2 cut(s) 150, 209
EarI CTCTTC 2 cut(s) 150, 209
Eco130I CCWWGG 2 cut(s) 129, 150
Eco24I GRGCYC 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 53
EcoT14I CCWWGG 2 cut(s) 129, 150
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 129, 150
FaeI CATG 3 cut(s) 22, 175, 272
FaiI YATR 7 cut(s) 20, 39, 164, 173, 223, 270, 307
FaqI GGGAC 1 cut(s) 221
FatI CATG 3 cut(s) 18, 171, 268
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 67, 251
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 2 cut(s) 67, 251
FspBI CTAG 1 cut(s) 26
GluI GCNGC 2 cut(s) 67, 251
HaeIII GGCC 2 cut(s) 55, 128
HapII CCGG 1 cut(s) 230
Hin1II CATG 3 cut(s) 22, 175, 272
HinfI GANTC 2 cut(s) 46, 287
HpaII CCGG 1 cut(s) 230
HphI GGTGA 1 cut(s) 199
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 1 cut(s) 269
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 2 cut(s) 76, 178
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 146
HpySE526I ACGT 2 cut(s) 76, 178
Hsp92II CATG 3 cut(s) 22, 175, 272
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 2 cut(s) 9, 265
LmnI GCTCC 2 cut(s) 63, 247
LpnPI CCDG 3 cut(s) 230, 239, 243
Lsp1109I GCAGC 2 cut(s) 53, 262
MaeI CTAG 1 cut(s) 26
MaeII ACGT 2 cut(s) 76, 178
MalI GATC 2 cut(s) 11, 267
MboI GATC 2 cut(s) 9, 265
MboII GAAGA 3 cut(s) 167, 173, 196
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 2 cut(s) 212, 291
MlyI GAGTC 1 cut(s) 55
MnlI CCTC 4 cut(s) 8, 106, 127, 272
MroNI GCCGGC 1 cut(s) 229
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 1 cut(s) 230
MunI CAATTG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 125, 146
NaeI GCCGGC 1 cut(s) 231
NdeII GATC 2 cut(s) 9, 265
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 3 cut(s) 22, 175, 272
NlaIV GGNNCC 1 cut(s) 55
PagI TCATGA 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 82
PdiI GCCGGC 1 cut(s) 231
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 2 cut(s) 68, 252
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 55
PspOMI GGGCCC 1 cut(s) 53
PspPI GGNCC 2 cut(s) 53, 54
PsuI RGATCY 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 273
SatI GCNGC 2 cut(s) 67, 251
Sau3AI GATC 2 cut(s) 9, 265
Sau96I GGNCC 2 cut(s) 53, 54
SchI GAGTC 1 cut(s) 55
SduI GDGCHC 1 cut(s) 57
SetI ASST 5 cut(s) 34, 68, 79, 91, 181
SmiMI CAYNNNNRTG 1 cut(s) 273
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 2 cut(s) 212, 291
SspMI CTAG 1 cut(s) 26
StyI CCWWGG 2 cut(s) 129, 150
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 2 cut(s) 79, 181
TasI AATT 2 cut(s) 212, 291
TfiI GAWTC 1 cut(s) 287
TseI GCWGC 2 cut(s) 66, 250
TspDTI ATGAA 2 cut(s) 17, 294
XapI RAATTY 1 cut(s) 212
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.