FvH4_4g08911

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000776
Physical Location & Seq
Forward (+)
9858420 .. 9862067
3648 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08911.t1

Sequence Viewer

Length: 1104 bp
ATGCAGGAGGTAGCCCAGCAATATCGACGCCCCACAGAGGTCCCCACGCGGCCTATGGCGCCTCCATCAAGTTCGGCACCTCCTCTCCCTCCACCACTGCCCGATGTCGACGAACCTTCCCTAGATGATCATCGGACTAGCCAGTCATCTGCTAAGATCAGCGTTCGGCGCAAACTGCGTTCTGAAGGAGCACCTCCCCCTAAAACCAAGACATGGGTGGTAAAAGGTTTAAAAATGGAGGATATAGTCAAGAGGACTGGATTCAAGATTAAAGTAACTTGGGACGATTTCTGTCACTGTCCGAAGAGTAAAGAGTTAAGCAGCATGTTGACACACGATGTCGGCCACATTATACGGGACGAAATTTCCATGGTGGCTCCAACTTGGGGTGACACTATTGATGATGAGCAGAAGAAGCTGCCCACTAAACTCTTTATCTTTTATGAGTTTGATATTGATGATAAGCTCATGTGGAAGTGGTTGGACGACAAGGCTAAAAATGTGTACAAAAACTGGAAGTGCACTATAAATACAATTAGGAAGGAAAACCCGGGGAAGATCCCAGAAGAGTTTGAACACCGGCTAGATGAGTGGAACTGGCACCTTAAACACTTTGAGTCTGATGGATTTACGCTGGCTATTGCTAGTTGGTGCCTTTGTTGTTCTGACCATTTGACTTTGGAAAACCAGTTTCTTGGAGGCTATAGTTTCTTGAGTTCTTCATTAATTAGTATCACACCAGCAGCTAAACTGATGGAGAAGGTTGATGAGATCCGTGACAATGTGAGGAAGGAATACCCCGGCATTCCGGAAGAAAATATTGATCTTCATTTTGACCAAGAAACTCAAGCGATCGTGTTTGAAAAAGCATTTGGAGATAGAAACATGAACGAGATCCGGGGCATGGGACTAGTAGGCAGTTACGGTTTGAGTCAAGGGCCCGATAAGAGCTGCATTTCACGTTCCCAACGAGCTGCACTTGTTGATATTGCTGTGGAGGAAGCCCGAAAGGCCAAGGAGGAAGCCCGGAAAGCCAATGAGGAAGCCCGAAAAGCTAAGGAAGAGAACATACAAATCATGGCACGTCTTCAACAATGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

368

Amino Acids

42.07

Weight (kDa)

5.95

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 142
AccB1I GGYRCC 4 cut(s) 58, 76, 600, 651
AccB7I CCANNNNNTGG 1 cut(s) 213
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 49
AccIII TCCGGA 1 cut(s) 808
AciI CCGC 1 cut(s) 49
AclWI GGATC 3 cut(s) 553, 766, 889
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 204
AcyI GRCGYC 2 cut(s) 28, 59
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 4 cut(s) 37, 213, 386, 904
AgsI TTSAA 4 cut(s) 265, 575, 863, 1091
AhlI ACTAGT 1 cut(s) 910
AjiI CACGTC 1 cut(s) 1085
AjuI GAANNNNNNNTTGG 2 cut(s) 855, 887
AluBI AGCT 6 cut(s) 418, 466, 746, 951, 974, 1055
AluI AGCT 6 cut(s) 418, 466, 746, 951, 974, 1055
Alw21I GWGCWC 2 cut(s) 193, 524
Alw44I GTGCAC 1 cut(s) 520
AlwI GGATC 3 cut(s) 553, 766, 889
Ama87I CYCGRG 1 cut(s) 550
Aor13HI TCCGGA 1 cut(s) 808
AoxI GGCC 4 cut(s) 50, 343, 938, 1011
ApaI GGGCCC 1 cut(s) 942
ApaLI GTGCAC 1 cut(s) 520
ApeKI GCWGC 5 cut(s) 321, 418, 743, 951, 974
ApoI RAATTY 1 cut(s) 363
AseI ATTAAT 1 cut(s) 725
AspLEI GCGC 2 cut(s) 61, 171
AspS9I GGNCC 3 cut(s) 40, 938, 939
AsuC2I CCSGG 5 cut(s) 551, 552, 801, 899, 1027
AsuHPI GGTGA 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 1 cut(s) 40
BaeGI GKGCMC 2 cut(s) 524, 942
BanI GGYRCC 4 cut(s) 58, 76, 600, 651
BanII GRGCYC 1 cut(s) 942
BarI GAAGNNNNNNTAC 2 cut(s) 1053, 1085
BbsI GAAGAC 1 cut(s) 1079
Bbv12I GWGCWC 2 cut(s) 193, 524
BbvI GCAGC 5 cut(s) 333, 405, 755, 938, 961
BccI CCATC 3 cut(s) 73, 617, 748
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 5 cut(s) 551, 552, 801, 899, 1027
BcuI ACTAGT 1 cut(s) 910
BfaI CTAG 5 cut(s) 122, 138, 584, 645, 911
BfmI CTRYAG 1 cut(s) 703
BfoI RGCGCY 1 cut(s) 62
BglI GCCNNNNNGGC 1 cut(s) 1010
BisI GCNGC 6 cut(s) 50, 322, 419, 744, 952, 975
BlsI GCNGC 6 cut(s) 51, 323, 420, 745, 953, 976
Bme1390I CCNGG 5 cut(s) 551, 552, 801, 899, 1027
Bme18I GGWCC 1 cut(s) 40
BmeT110I CYCGRG 1 cut(s) 550
BmgBI CACGTC 1 cut(s) 1085
BmgT120I GGNCC 3 cut(s) 40, 938, 939
BmiI GGNNCC 7 cut(s) 42, 60, 78, 378, 602, 653, 940
BmrFI CCNGG 5 cut(s) 551, 552, 801, 899, 1027
BpiI GAAGAC 1 cut(s) 1079
Bpu10I CCTNAGC 1 cut(s) 1056
BpuEI CTTGAG 2 cut(s) 733, 831
BpuMI CCSGG 5 cut(s) 551, 552, 801, 899, 1027
BsaBI GATNNNNATC 1 cut(s) 129
BsaHI GRCGYC 2 cut(s) 28, 59
BsaJI CCNNGG 6 cut(s) 369, 550, 551, 799, 898, 1014
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 213, 386, 904
Bse118I RCCGGY 1 cut(s) 579
Bse1I ACTGG 5 cut(s) 142, 262, 518, 602, 688
Bse8I GATNNNNATC 1 cut(s) 129
BseAI TCCGGA 1 cut(s) 808
BseDI CCNNGG 6 cut(s) 369, 550, 551, 799, 898, 1014
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 4 cut(s) 37, 213, 386, 904
BseNI ACTGG 5 cut(s) 142, 262, 518, 602, 688
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 2 cut(s) 524, 942
BseXI GCAGC 5 cut(s) 333, 405, 755, 938, 961
BseYI CCCAGC 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 1 cut(s) 49
Bsh1285I CGRYCG 1 cut(s) 855
BshFI GGCC 4 cut(s) 52, 345, 940, 1013
BshNI GGYRCC 4 cut(s) 58, 76, 600, 651
BsiEI CGRYCG 1 cut(s) 855
BsiHKAI GWGCWC 2 cut(s) 193, 524
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 6 cut(s) 551, 580, 801, 809, 898, 1027
BslFI GGGAC 4 cut(s) 26, 296, 371, 921
BslI CCNNNNNNNGG 4 cut(s) 37, 213, 386, 904
BsmFI GGGAC 4 cut(s) 26, 296, 371, 921
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 4 cut(s) 52, 345, 940, 1013
BsoBI CYCGRG 1 cut(s) 550
Bsp120I GGGCCC 1 cut(s) 938
Bsp1286I GDGCHC 3 cut(s) 193, 524, 942
Bsp13I TCCGGA 1 cut(s) 808
Bsp1407I TGTACA 1 cut(s) 504
Bsp143I GATC 7 cut(s) 127, 156, 558, 771, 823, 852, 894
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 1 cut(s) 49
BspANI GGCC 4 cut(s) 52, 345, 940, 1013
BspEI TCCGGA 1 cut(s) 808
BspFNI CGCG 1 cut(s) 49
BspLI GGNNCC 7 cut(s) 42, 60, 78, 378, 602, 653, 940
BspPI GGATC 3 cut(s) 553, 766, 889
BspT107I GGYRCC 4 cut(s) 58, 76, 600, 651
BsrFI RCCGGY 1 cut(s) 579
BsrGI TGTACA 1 cut(s) 504
BsrI ACTGG 5 cut(s) 142, 262, 518, 602, 688
BssAI RCCGGY 1 cut(s) 579
BssECI CCNNGG 6 cut(s) 369, 550, 551, 799, 898, 1014
BssMI GATC 7 cut(s) 127, 156, 558, 771, 823, 852, 894
BssNI GRCGYC 2 cut(s) 28, 59
BssT1I CCWWGG 2 cut(s) 369, 1014
Bst4CI ACNGT 2 cut(s) 299, 926
Bst6I CTCTTC 3 cut(s) 299, 561, 1056
BstACI GRCGYC 2 cut(s) 28, 59
BstAUI TGTACA 1 cut(s) 504
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 2 cut(s) 153, 1056
BstDSI CCRYGG 1 cut(s) 369
BstFNI CGCG 1 cut(s) 49
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 2 cut(s) 61, 171
BstKTI GATC 7 cut(s) 130, 159, 561, 774, 826, 855, 897
BstMBI GATC 7 cut(s) 127, 156, 558, 771, 823, 852, 894
BstMCI CGRYCG 1 cut(s) 855
BstMWI GCNNNNNNNGC 7 cut(s) 58, 168, 175, 415, 1010, 1031, 1052
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 5 cut(s) 549, 550, 799, 897, 1025
BstSFI CTRYAG 1 cut(s) 703
BstSLI GKGCMC 2 cut(s) 524, 942
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 5 cut(s) 333, 405, 755, 938, 961
BstV2I GAAGAC 1 cut(s) 1079
BstX2I RGATCY 3 cut(s) 558, 771, 894
BstXI CCANNNNNNTGG 1 cut(s) 695
BstYI RGATCY 3 cut(s) 558, 771, 894
BsuRI GGCC 4 cut(s) 52, 345, 940, 1013
BtgI CCRYGG 1 cut(s) 369
BtrI CACGTC 1 cut(s) 1085
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 95, 295
Cac8I GCNNGC 1 cut(s) 636
CfoI GCGC 2 cut(s) 61, 171
Cfr10I RCCGGY 1 cut(s) 579
Cfr13I GGNCC 3 cut(s) 40, 938, 939
Cfr9I CCCGGG 1 cut(s) 550
CseI GACGC 1 cut(s) 36
Csp6I GTAC 1 cut(s) 505
CviAII CATG 7 cut(s) 213, 325, 370, 469, 886, 904, 1078
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 2 cut(s) 153, 1056
DinI GGCGCC 1 cut(s) 60
DpnI GATC 7 cut(s) 129, 158, 560, 773, 825, 854, 896
DpnII GATC 7 cut(s) 127, 156, 558, 771, 823, 852, 894
DraI TTTAAA 1 cut(s) 231
DrdI GACNNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 3 cut(s) 299, 561, 1056
EarI CTCTTC 3 cut(s) 299, 561, 1056
Eco130I CCWWGG 2 cut(s) 369, 1014
Eco24I GRGCYC 1 cut(s) 942
Eco47I GGWCC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 550
EcoO109I RGGNCCY 2 cut(s) 40, 938
EcoT14I CCWWGG 2 cut(s) 369, 1014
EcoT38I GRGCYC 1 cut(s) 942
EgeI GGCGCC 1 cut(s) 60
EheI GGCGCC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 369, 1014
FaeI CATG 7 cut(s) 216, 328, 373, 472, 889, 907, 1081
FaqI GGGAC 4 cut(s) 26, 296, 371, 921
FatI CATG 7 cut(s) 212, 324, 369, 468, 885, 903, 1077
FbaI TGATCA 1 cut(s) 127
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 6 cut(s) 50, 322, 419, 744, 952, 975
FriOI GRGCYC 1 cut(s) 942
Fsp4HI GCNGC 6 cut(s) 50, 322, 419, 744, 952, 975
FspBI CTAG 5 cut(s) 122, 138, 584, 645, 911
GlaI GCGC 2 cut(s) 60, 170
GluI GCNGC 6 cut(s) 50, 322, 419, 744, 952, 975
GsaI CCCAGC 1 cut(s) 19
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 4 cut(s) 52, 345, 940, 1013
HapII CCGG 6 cut(s) 551, 580, 801, 809, 898, 1027
HgaI GACGC 1 cut(s) 36
HhaI GCGC 2 cut(s) 61, 171
Hin1I GRCGYC 2 cut(s) 28, 59
Hin1II CATG 7 cut(s) 216, 328, 373, 472, 889, 907, 1081
Hin6I GCGC 2 cut(s) 59, 169
HinP1I GCGC 2 cut(s) 59, 169
HincII GTYRAC 2 cut(s) 109, 330
HindII GTYRAC 2 cut(s) 109, 330
HinfI GANTC 3 cut(s) 261, 617, 931
HpaII CCGG 6 cut(s) 551, 580, 801, 809, 898, 1027
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 4 cut(s) 109, 330, 505, 522
Hpy188I TCNGA 5 cut(s) 135, 184, 303, 622, 667
Hpy188III TCNNGA 4 cut(s) 250, 265, 712, 809
Hpy8I GTNNAC 4 cut(s) 109, 330, 505, 522
Hpy99I CGWCG 2 cut(s) 30, 113
HpyAV CCTTC 5 cut(s) 126, 179, 535, 754, 784
HpyCH4III ACNGT 2 cut(s) 299, 926
HpyCH4IV ACGT 2 cut(s) 961, 1084
HpyCH4V TGCA 4 cut(s) 4, 522, 954, 977
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 168, 175, 415, 1010, 1031, 1052
HpyF3I CTNAG 2 cut(s) 153, 1056
HpySE526I ACGT 2 cut(s) 961, 1084
Hsp92I GRCGYC 2 cut(s) 28, 59
Hsp92II CATG 7 cut(s) 216, 328, 373, 472, 889, 907, 1081
HspAI GCGC 2 cut(s) 59, 169
KasI GGCGCC 1 cut(s) 58
Kpn2I TCCGGA 1 cut(s) 808
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 7 cut(s) 127, 156, 558, 771, 823, 852, 894
LmnI GCTCC 2 cut(s) 188, 382
Lsp1109I GCAGC 5 cut(s) 333, 405, 755, 938, 961
MaeI CTAG 5 cut(s) 122, 138, 584, 645, 911
MaeII ACGT 2 cut(s) 961, 1084
MaeIII GTNAC 5 cut(s) 274, 293, 389, 776, 920
MalI GATC 7 cut(s) 129, 158, 560, 773, 825, 854, 896
MboI GATC 7 cut(s) 127, 156, 558, 771, 823, 852, 894
MboII GAAGA 9 cut(s) 316, 424, 568, 578, 711, 818, 824, 1073, 1079
MflI RGATCY 3 cut(s) 558, 771, 894
MhlI GDGCHC 3 cut(s) 193, 524, 942
MluCI AATT 3 cut(s) 363, 534, 726
Mly113I GGCGCC 1 cut(s) 59
MlyI GAGTC 2 cut(s) 626, 940
MmeI TCCRAC 2 cut(s) 404, 462
MroI TCCGGA 1 cut(s) 808
MseI TTAA 5 cut(s) 230, 270, 317, 606, 725
MspI CCGG 6 cut(s) 551, 580, 801, 809, 898, 1027
MspR9I CCNGG 5 cut(s) 551, 552, 801, 899, 1027
Mva1269I GAATGC 1 cut(s) 804
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 7 cut(s) 58, 168, 175, 415, 1010, 1031, 1052
NarI GGCGCC 1 cut(s) 59
NciI CCSGG 5 cut(s) 551, 552, 801, 899, 1027
NcoI CCATGG 1 cut(s) 369
NdeII GATC 7 cut(s) 127, 156, 558, 771, 823, 852, 894
NlaIII CATG 7 cut(s) 216, 328, 373, 472, 889, 907, 1081
NlaIV GGNNCC 7 cut(s) 42, 60, 78, 378, 602, 653, 940
NmuCI GTSAC 3 cut(s) 293, 389, 776
NspI RCATGY 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 967
PctI GAATGC 1 cut(s) 804
PfeI GAWTC 1 cut(s) 261
PflMI CCANNNNNTGG 1 cut(s) 213
PkrI GCNGC 6 cut(s) 51, 323, 420, 745, 953, 976
Ple19I CGATCG 1 cut(s) 855
PleI GAGTC 2 cut(s) 625, 939
PluTI GGCGCC 1 cut(s) 62
PpsI GAGTC 2 cut(s) 625, 939
PpuMI RGGWCCY 1 cut(s) 40
PshBI ATTAAT 1 cut(s) 725
Psp5II RGGWCCY 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 15
PspN4I GGNNCC 7 cut(s) 42, 60, 78, 378, 602, 653, 940
PspOMI GGGCCC 1 cut(s) 938
PspPI GGNCC 3 cut(s) 40, 938, 939
PspPPI RGGWCCY 1 cut(s) 40
PsuI RGATCY 3 cut(s) 558, 771, 894
PvuI CGATCG 1 cut(s) 855
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SalI GTCGAC 1 cut(s) 107
SaqAI TTAA 5 cut(s) 230, 270, 317, 606, 725
SatI GCNGC 6 cut(s) 50, 322, 419, 744, 952, 975
Sau3AI GATC 7 cut(s) 127, 156, 558, 771, 823, 852, 894
Sau96I GGNCC 3 cut(s) 40, 938, 939
SchI GAGTC 2 cut(s) 626, 940
ScrFI CCNGG 5 cut(s) 551, 552, 801, 899, 1027
SduI GDGCHC 3 cut(s) 193, 524, 942
SfcI CTRYAG 1 cut(s) 703
SfoI GGCGCC 1 cut(s) 60
SinI GGWCC 1 cut(s) 40
SmaI CCCGGG 1 cut(s) 552
SmlI CTYRAG 2 cut(s) 712, 846
SmoI CTYRAG 2 cut(s) 712, 846
SpeI ACTAGT 1 cut(s) 910
Sse9I AATT 3 cut(s) 363, 534, 726
SsiI CCGC 1 cut(s) 49
SspDI GGCGCC 1 cut(s) 58
SspI AATATT 1 cut(s) 820
SspMI CTAG 5 cut(s) 122, 138, 584, 645, 911
StyD4I CCNGG 5 cut(s) 549, 550, 799, 897, 1025
StyI CCWWGG 2 cut(s) 369, 1014
TaaI ACNGT 2 cut(s) 299, 926
TaiI ACGT 2 cut(s) 964, 1087
TaqI TCGA 2 cut(s) 25, 108
TasI AATT 3 cut(s) 363, 534, 726
TatI WGTACW 1 cut(s) 504
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 5 cut(s) 230, 270, 317, 606, 725
Tru9I TTAA 5 cut(s) 230, 270, 317, 606, 725
TscAI CASTG 2 cut(s) 102, 302
TseFI GTSAC 3 cut(s) 293, 389, 776
TseI GCWGC 5 cut(s) 321, 418, 743, 951, 974
Tsp45I GTSAC 3 cut(s) 293, 389, 776
TspDTI ATGAA 3 cut(s) 711, 818, 902
TspGWI ACGGA 1 cut(s) 764
TspMI CCCGGG 1 cut(s) 550
TspRI CASTG 2 cut(s) 102, 302
Van91I CCANNNNNTGG 1 cut(s) 213
VneI GTGCAC 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 40
VspI ATTAAT 1 cut(s) 725
XapI RAATTY 1 cut(s) 363
XceI RCATGY 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 2 cut(s) 52, 214
XmaI CCCGGG 1 cut(s) 550
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 5 cut(s) 122, 138, 584, 645, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.