FvH4_4g10221

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000776
Physical Location & Seq
Reverse (-)
13397599 .. 13398454
856 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10221.t1

Sequence Viewer

Length: 603 bp
ATGGAGTGTGCGCAACAATACGGCTCCTCTTCTGCTGGATCCAACCAAGCCACTACGAGTACCGCGCCTGCAGGATCCCTTGCTTCGTCGGCTCCCCCTCTAACCGACGACGATGACAAGTTAACGACCCAAGTAGCAGAAACACCGGCTGAGGATGCACCTAGGGTTTTCAGCGCTCGCCGGAAACCGAAGAAAACCGGAGGACTTCCCCAGAAAGGCAATCGGGGTGTGGTGAACAACGCAAAGGTGATTGATATTGTGAAAAGCACAGGGAAGATGATCGACTTGGATTTTGATTGGGAAGTTATTGACGGACCTTCTAATTCTGATGTATGCAGTCTCTTTGTCAATAGTCTTGGAAGCATCATACGAAAAAGAGTGGCAATGAAGAAGGAATCGTGGTATGATTTAGACAAGGAGGAGAGGAAGAAGCTTTTTGACGGTCTCACTCACGACTTGTCCGAGATTCACAAGCTCGGGGGGAGAGATGTGATTCTGGAAGAGCTTTTGCACAGGTGGGATGAGTGGGAGTGGCTTTGCACGCATTTTGATTCGGACAAGTACAAGTTGGGAATTTGGATTAAAATTGGATATGATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.26

Weight (kDa)

5.44

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 12
AccII CGCG 1 cut(s) 65
AciI CCGC 1 cut(s) 63
AclWI GGATC 4 cut(s) 33, 46, 69, 82
AcsI RAATTY 1 cut(s) 573
AfaI GTAC 2 cut(s) 61, 563
AfeI AGCGCT 1 cut(s) 175
AfiI CCNNNNNNNGG 1 cut(s) 215
AluBI AGCT 3 cut(s) 433, 475, 505
AluI AGCT 3 cut(s) 433, 475, 505
Alw26I GTCTC 2 cut(s) 344, 449
AlwI GGATC 4 cut(s) 33, 46, 69, 82
Ama87I CYCGRG 1 cut(s) 476
Aor51HI AGCGCT 1 cut(s) 175
ApoI RAATTY 1 cut(s) 573
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspA2I CCTAGG 1 cut(s) 161
AspLEI GCGC 3 cut(s) 13, 67, 176
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 2 cut(s) 244, 259
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 1 cut(s) 314
AvrII CCTAGG 1 cut(s) 161
BamHI GGATCC 2 cut(s) 38, 74
BbvCI CCTCAGC 1 cut(s) 150
BceAI ACGGC 1 cut(s) 37
BcoDI GTCTC 2 cut(s) 344, 449
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 177
BlnI CCTAGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 314
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 4 cut(s) 25, 40, 76, 93
BmsI GCATC 2 cut(s) 145, 372
Bpu10I CCTNAGC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse118I RCCGGY 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 2 cut(s) 160, 526
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 141
BseRI GAGGAG 2 cut(s) 16, 434
Bsh1236I CGCG 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 3 cut(s) 146, 181, 198
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 2 cut(s) 344, 449
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 476
Bsp143I GATC 3 cut(s) 38, 74, 279
BspACI CCGC 1 cut(s) 63
BspCNI CTCAG 1 cut(s) 142
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 4 cut(s) 25, 40, 76, 93
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 4 cut(s) 33, 46, 69, 82
BspQI GCTCTTC 1 cut(s) 495
BspTNI GGTCTC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 3 cut(s) 38, 74, 279
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 443
Bst6I CTCTTC 2 cut(s) 34, 495
BstC8I GCNNGC 3 cut(s) 69, 178, 542
BstDEI CTNAG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 160, 526
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 3 cut(s) 13, 67, 176
BstKTI GATC 3 cut(s) 41, 77, 282
BstMAI GTCTC 2 cut(s) 344, 449
BstMBI GATC 3 cut(s) 38, 74, 279
BstMWI GCNNNNNNNGC 3 cut(s) 89, 155, 541
BstSFI CTRYAG 1 cut(s) 69
BstUI CGCG 1 cut(s) 65
BstX2I RGATCY 2 cut(s) 38, 74
BstYI RGATCY 2 cut(s) 38, 74
BtsCI GGATG 2 cut(s) 160, 526
Cac8I GCNNGC 3 cut(s) 69, 178, 542
CfoI GCGC 3 cut(s) 13, 67, 176
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 60, 562
CviJI RGCY 8 cut(s) 24, 50, 92, 149, 433, 475, 505, 535
CviKI_1 RGCY 8 cut(s) 24, 50, 92, 149, 433, 475, 505, 535
CviQI GTAC 2 cut(s) 60, 562
DdeI CTNAG 1 cut(s) 150
DpnI GATC 3 cut(s) 40, 76, 281
DpnII GATC 3 cut(s) 38, 74, 279
Eam1104I CTCTTC 2 cut(s) 34, 495
EarI CTCTTC 2 cut(s) 34, 495
Eco130I CCWWGG 1 cut(s) 161
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 1 cut(s) 314
Eco47III AGCGCT 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
FaiI YATR 4 cut(s) 334, 368, 405, 594
FokI GGATG 2 cut(s) 167, 533
FspBI CTAG 1 cut(s) 162
FspI TGCGCA 1 cut(s) 12
GlaI GCGC 3 cut(s) 12, 66, 175
HaeII RGCGCY 1 cut(s) 177
HapII CCGG 3 cut(s) 146, 181, 198
HhaI GCGC 3 cut(s) 13, 67, 176
Hin6I GCGC 3 cut(s) 11, 65, 174
HinP1I GCGC 3 cut(s) 11, 65, 174
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 4 cut(s) 395, 466, 493, 551
HpaI GTTAAC 1 cut(s) 123
HpaII CCGG 3 cut(s) 146, 181, 198
HphI GGTGA 2 cut(s) 244, 259
Hpy166II GTNNAC 2 cut(s) 123, 235
Hpy188I TCNGA 3 cut(s) 328, 463, 556
Hpy188III TCNNGA 2 cut(s) 452, 497
Hpy8I GTNNAC 2 cut(s) 123, 235
Hpy99I CGWCG 3 cut(s) 91, 110, 113
HpyAV CCTTC 2 cut(s) 327, 385
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4V TGCA 5 cut(s) 71, 158, 336, 511, 540
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 155, 541
HpyF3I CTNAG 1 cut(s) 150
HspAI GCGC 3 cut(s) 11, 65, 174
KspAI GTTAAC 1 cut(s) 123
Kzo9I GATC 3 cut(s) 38, 74, 279
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 29, 97
LweI GCATC 2 cut(s) 145, 372
MaeI CTAG 1 cut(s) 162
MalI GATC 3 cut(s) 40, 76, 281
MboI GATC 3 cut(s) 38, 74, 279
MboII GAAGA 6 cut(s) 21, 202, 286, 400, 439, 512
MflI RGATCY 2 cut(s) 38, 74
MluCI AATT 3 cut(s) 322, 573, 585
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 6 cut(s) 37, 108, 145, 194, 412, 417
MseI TTAA 2 cut(s) 122, 582
MspI CCGG 3 cut(s) 146, 181, 198
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 3 cut(s) 89, 155, 541
NdeII GATC 3 cut(s) 38, 74, 279
NlaIV GGNNCC 4 cut(s) 25, 40, 76, 93
NsbI TGCGCA 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 495
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PfeI GAWTC 4 cut(s) 395, 466, 493, 551
PspN4I GGNNCC 4 cut(s) 25, 40, 76, 93
PspPI GGNCC 1 cut(s) 314
PstI CTGCAG 1 cut(s) 73
PsuI RGATCY 2 cut(s) 38, 74
RsaI GTAC 2 cut(s) 61, 563
RsaNI GTAC 2 cut(s) 60, 562
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 2 cut(s) 122, 582
Sau3AI GATC 3 cut(s) 38, 74, 279
Sau96I GGNCC 1 cut(s) 314
SbfI CCTGCAGG 1 cut(s) 73
SdaI CCTGCAGG 1 cut(s) 73
SetI ASST 7 cut(s) 163, 249, 319, 435, 477, 507, 518
SfaNI GCATC 2 cut(s) 145, 372
SfcI CTRYAG 1 cut(s) 69
SinI GGWCC 1 cut(s) 314
Sse8387I CCTGCAGG 1 cut(s) 73
Sse9I AATT 3 cut(s) 322, 573, 585
SsiI CCGC 1 cut(s) 63
SspMI CTAG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 1 cut(s) 443
TaqI TCGA 1 cut(s) 282
TasI AATT 3 cut(s) 322, 573, 585
TatI WGTACW 1 cut(s) 561
TfiI GAWTC 4 cut(s) 395, 466, 493, 551
Tru1I TTAA 2 cut(s) 122, 582
Tru9I TTAA 2 cut(s) 122, 582
TspDTI ATGAA 1 cut(s) 401
TspGWI ACGGA 1 cut(s) 327
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 1 cut(s) 573
XmaJI CCTAGG 1 cut(s) 161
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.