FvH4_6g24310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000776
Physical Location & Seq
Forward (+)
18387024 .. 18387825
802 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24310.t1

Sequence Viewer

Length: 411 bp
ATGCAGAAAGAGGCCACTGAATTGAAAAATGCACAGAAGAATGCAAACCCTGACATCCCGGAGGCTGAGCTCCCACCTTTGGAGTGGTCAATGAATCTTAAGGTGCTTGAAGCAGGAGTCGGCAGAAGAGCCGAGAAAGAGATCCGTGGTCTTGGAAGAGCAGGTGTCCGACAGTCATTATCTTCCATTCCACATTCTCGAGTTCATGAGGTGGATGAGAGATTACATATTACTGAGGAGATGGCCGAAAAAGCTGAGGCGGTTGCGCAAAACGTTAGTGAAAAGATGACATTGGTGAAGCAGCAACTAGCAGCGGTGATGTCTTTTATGGCACAGCAGACTGGGTGTCCTCCACCAGTCCCTTCCCAACAACCTCTAGAAGAAAATGTTAATGCAGCTGAGAGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.02

Weight (kDa)

5.45

Isoelectric Point (pI)

56.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 152
Acc16I TGCGCA 1 cut(s) 267
Acc36I ACCTGC 1 cut(s) 152
AciI CCGC 2 cut(s) 260, 314
AclI AACGTT 1 cut(s) 273
AclWI GGATC 1 cut(s) 136
AcoI YGGCCR 1 cut(s) 243
AfiI CCNNNNNNNGG 1 cut(s) 79
AflII CTTAAG 1 cut(s) 98
AgsI TTSAA 2 cut(s) 25, 110
AhdI GACNNNNNGTC 1 cut(s) 345
AluBI AGCT 3 cut(s) 70, 254, 398
AluI AGCT 3 cut(s) 70, 254, 398
Alw21I GWGCWC 1 cut(s) 72
AlwI GGATC 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 2 cut(s) 12, 243
ApeKI GCWGC 3 cut(s) 301, 311, 395
AspLEI GCGC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 307, 328
AvaI CYCGRG 1 cut(s) 198
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 255
BbvI GCAGC 3 cut(s) 313, 323, 407
BccI CCATC 1 cut(s) 235
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 3 cut(s) 308, 377, 409
BfrI CTTAAG 1 cut(s) 98
BfuAI ACCTGC 1 cut(s) 152
BisI GCNGC 3 cut(s) 302, 312, 396
BlpI GCTNAGC 1 cut(s) 66
BlsI GCNGC 3 cut(s) 303, 313, 397
Bme1390I CCNGG 1 cut(s) 59
BmeRI GACNNNNNGTC 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 59
BmrI ACTGGG 1 cut(s) 351
BmuI ACTGGG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 255
Bpu1102I GCTNAGC 1 cut(s) 66
BpuMI CCSGG 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 346, 356
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 2 cut(s) 54, 220
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMII CTCAG 4 cut(s) 57, 225, 246, 390
BseNI ACTGG 2 cut(s) 346, 356
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 3 cut(s) 313, 323, 407
BshFI GGCC 2 cut(s) 14, 245
BsiHKAI GWGCWC 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 2 cut(s) 14, 245
BsoBI CYCGRG 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 1 cut(s) 141
Bsp1720I GCTNAGC 1 cut(s) 66
BspACI CCGC 2 cut(s) 260, 314
BspANI GGCC 2 cut(s) 14, 245
BspCNI CTCAG 4 cut(s) 58, 226, 247, 391
BspHI TCATGA 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 152
BspPI GGATC 1 cut(s) 136
BspQI GCTCTTC 2 cut(s) 121, 151
BspTI CTTAAG 1 cut(s) 98
BsrI ACTGG 2 cut(s) 346, 356
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 2 cut(s) 121, 151
BstAFI CTTAAG 1 cut(s) 98
BstDEI CTNAG 4 cut(s) 66, 234, 255, 399
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 2 cut(s) 54, 220
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 1 cut(s) 144
BstMBI GATC 1 cut(s) 141
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 313, 323, 407
BstX2I RGATCY 1 cut(s) 141
BstYI RGATCY 1 cut(s) 141
BsuRI GGCC 2 cut(s) 14, 245
BtgI CCRYGG 1 cut(s) 145
BtsCI GGATG 2 cut(s) 54, 220
BtsIMutI CAGTG 1 cut(s) 15
BveI ACCTGC 1 cut(s) 152
CciI TCATGA 1 cut(s) 205
CfoI GCGC 1 cut(s) 268
CviAII CATG 1 cut(s) 206
CviJI RGCY 7 cut(s) 14, 65, 70, 131, 245, 254, 398
CviKI_1 RGCY 7 cut(s) 14, 65, 70, 131, 245, 254, 398
DdeI CTNAG 4 cut(s) 66, 234, 255, 399
DpnI GATC 1 cut(s) 143
DpnII GATC 1 cut(s) 141
DriI GACNNNNNGTC 1 cut(s) 345
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 121, 151
Eam1105I GACNNNNNGTC 1 cut(s) 345
EarI CTCTTC 2 cut(s) 121, 151
Ecl136II GAGCTC 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 198
EcoICRI GAGCTC 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 72
FaeI CATG 1 cut(s) 209
FaiI YATR 3 cut(s) 207, 228, 329
FaqI GGGAC 1 cut(s) 344
FatI CATG 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 302, 312, 396
FokI GGATG 2 cut(s) 41, 227
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 3 cut(s) 302, 312, 396
FspBI CTAG 3 cut(s) 308, 377, 409
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 1 cut(s) 267
GluI GCNGC 3 cut(s) 302, 312, 396
HaeIII GGCC 2 cut(s) 14, 245
HapII CCGG 1 cut(s) 59
HhaI GCGC 1 cut(s) 268
Hin1II CATG 1 cut(s) 209
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HinfI GANTC 2 cut(s) 94, 117
HpaII CCGG 1 cut(s) 59
HphI GGTGA 2 cut(s) 307, 328
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 3 cut(s) 198, 206, 377
HpyAV CCTTC 1 cut(s) 372
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 4 cut(s) 4, 32, 44, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 4 cut(s) 66, 234, 255, 399
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 1 cut(s) 209
HspAI GCGC 1 cut(s) 266
Kzo9I GATC 1 cut(s) 141
LguI GCTCTTC 2 cut(s) 121, 151
LmnI GCTCC 1 cut(s) 75
LpnPI CCDG 6 cut(s) 63, 72, 99, 147, 327, 369
Lsp1109I GCAGC 3 cut(s) 313, 323, 407
MaeI CTAG 3 cut(s) 308, 377, 409
MaeII ACGT 1 cut(s) 273
MalI GATC 1 cut(s) 143
MboI GATC 1 cut(s) 141
MboII GAAGA 5 cut(s) 49, 138, 168, 174, 392
MflI RGATCY 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 1 cut(s) 20
MlyI GAGTC 1 cut(s) 126
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 8 cut(s) 4, 55, 202, 229, 250, 360, 384, 396
MseI TTAA 2 cut(s) 99, 390
MspA1I CMGCKG 2 cut(s) 314, 398
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 46
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 1 cut(s) 59
NdeII GATC 1 cut(s) 141
NlaIII CATG 1 cut(s) 209
NmeAIII GCCGAG 1 cut(s) 157
NsbI TGCGCA 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 198
PagI TCATGA 1 cut(s) 205
PaqCI CACCTGC 1 cut(s) 152
PciSI GCTCTTC 2 cut(s) 121, 151
PctI GAATGC 1 cut(s) 46
PfeI GAWTC 1 cut(s) 94
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 3 cut(s) 303, 313, 397
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
Psp124BI GAGCTC 1 cut(s) 72
Psp1406I AACGTT 1 cut(s) 273
PsuI RGATCY 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 72
SapI GCTCTTC 2 cut(s) 121, 151
SaqAI TTAA 2 cut(s) 99, 390
SatI GCNGC 3 cut(s) 302, 312, 396
Sau3AI GATC 1 cut(s) 141
SchI GAGTC 1 cut(s) 126
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 1 cut(s) 72
SetI ASST 9 cut(s) 72, 79, 105, 166, 213, 256, 276, 376, 400
Sfr274I CTCGAG 1 cut(s) 198
SlaI CTCGAG 1 cut(s) 198
SmlI CTYRAG 2 cut(s) 98, 198
SmoI CTYRAG 2 cut(s) 98, 198
Sse9I AATT 1 cut(s) 20
SsiI CCGC 2 cut(s) 260, 314
SspMI CTAG 3 cut(s) 308, 377, 409
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 174
TaiI ACGT 1 cut(s) 276
TaqI TCGA 1 cut(s) 199
TasI AATT 1 cut(s) 20
TfiI GAWTC 1 cut(s) 94
Tru1I TTAA 2 cut(s) 99, 390
Tru9I TTAA 2 cut(s) 99, 390
TscAI CASTG 1 cut(s) 22
TseI GCWGC 3 cut(s) 301, 311, 395
TspDTI ATGAA 2 cut(s) 107, 194
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 1 cut(s) 22
Vha464I CTTAAG 1 cut(s) 98
XbaI TCTAGA 1 cut(s) 376
XcmI CCANNNNNNNNNTGG 1 cut(s) 81
XhoI CTCGAG 1 cut(s) 198
XspI CTAG 3 cut(s) 308, 377, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.