FvH4_5g32550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000776
Physical Location & Seq
Reverse (-)
23497859 .. 23498918
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32550.t1

Sequence Viewer

Length: 516 bp
ATGGACGAGTGGAATTGGCTTAAGCAACACTTCTTGTCACCTCAGTTTCAGAAACTCTCAGCTGCAAACTTGGCAAATCGAGACAAATTAACCATGCATCACCACGCCGGTTGTACAGCTTTCATATACAATGCAGACAAACTTAGGCGTGAGGGTGAATCATTATACCTTATCAACGGTTTTGAGGTAGCGTACGACACCCCACATAACGAGATTGCAGCCGAAAAAGCCAACGATATGAGACAGAAAATCCTAACAATGCTAAGCGAACAACATCTGGACACTCCAGAGGATGAGCTCCTTCAACAAGTAGACTTTCGATCTCAGGTTGACGCATTTGAGAATGTCCTTGGGACCAGGGGGGGGGGAATAAAGGGGCTTGGGAGAGTTGAAATTGTGGGCAAGGGAAAAGCAGCAAGGCGTTCTCGTCCCAATGATATAGTTCTTGAGGAGGTTCAGGAGCATGCCCGACAAGCCCAAAAGGAGGCACGACATGCTCAAGAGGAGGCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

172

Amino Acids

19.5

Weight (kDa)

5.9

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 312
AfaI GTAC 2 cut(s) 115, 194
AfiI CCNNNNNNNGG 2 cut(s) 363, 484
AflII CTTAAG 1 cut(s) 20
AgsI TTSAA 2 cut(s) 305, 392
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 3 cut(s) 62, 119, 298
AluI AGCT 3 cut(s) 62, 119, 298
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 2 cut(s) 75, 235
ApeKI GCWGC 3 cut(s) 62, 218, 413
AspS9I GGNCC 1 cut(s) 354
AsuHPI GGTGA 3 cut(s) 30, 92, 167
AvaII GGWCC 1 cut(s) 354
BanII GRGCYC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 49, 230, 425
BciT130I CCWGG 1 cut(s) 358
BcoDI GTCTC 2 cut(s) 75, 235
BfrI CTTAAG 1 cut(s) 20
BisI GCNGC 3 cut(s) 63, 219, 414
BlpI GCTNAGC 1 cut(s) 263
BlsI GCNGC 3 cut(s) 64, 220, 415
Bme1390I CCNGG 1 cut(s) 358
Bme18I GGWCC 1 cut(s) 354
BmgT120I GGNCC 1 cut(s) 354
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 358
BmsI GCATC 1 cut(s) 106
BpmI CTGGAG 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 263
BpuEI CTTGAG 2 cut(s) 467, 483
BsaJI CCNNGG 2 cut(s) 349, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 363, 484
Bse118I RCCGGY 1 cut(s) 107
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 2 cut(s) 349, 357
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 2 cut(s) 363, 484
BseMII CTCAG 3 cut(s) 56, 72, 338
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 3 cut(s) 49, 230, 425
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 1 cut(s) 108
BsiWI CGTACG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 367, 414
BslI CCNNNNNNNGG 2 cut(s) 363, 484
BsmAI GTCTC 2 cut(s) 75, 235
BsmFI GGGAC 2 cut(s) 367, 414
Bsp1286I GDGCHC 1 cut(s) 300
Bsp1407I TGTACA 1 cut(s) 113
Bsp143I GATC 1 cut(s) 320
Bsp1720I GCTNAGC 1 cut(s) 263
BspCNI CTCAG 3 cut(s) 55, 71, 337
BspLI GGNNCC 1 cut(s) 355
BspTI CTTAAG 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 107
BsrGI TGTACA 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 349, 357
BssMI GATC 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 1 cut(s) 179
BstAFI CTTAAG 1 cut(s) 20
BstAPI GCANNNNNTGC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 465
BstDEI CTNAG 5 cut(s) 42, 58, 143, 263, 324
BstF5I GGATG 1 cut(s) 298
BstKTI GATC 1 cut(s) 323
BstMAI GTCTC 2 cut(s) 75, 235
BstMBI GATC 1 cut(s) 320
BstMWI GCNNNNNNNGC 4 cut(s) 71, 227, 473, 494
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 2 cut(s) 467, 497
BstSCI CCNGG 1 cut(s) 356
BstV1I GCAGC 3 cut(s) 49, 230, 425
BtsCI GGATG 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 465
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 354
CseI GACGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 114, 193
CspCI CAANNNNNGTGG 2 cut(s) 92, 127
CviAII CATG 3 cut(s) 94, 464, 494
CviJI RGCY 8 cut(s) 19, 62, 119, 221, 230, 298, 379, 476
CviKI_1 RGCY 8 cut(s) 19, 62, 119, 221, 230, 298, 379, 476
CviQI GTAC 2 cut(s) 114, 193
DdeI CTNAG 5 cut(s) 42, 58, 143, 263, 324
DpnI GATC 1 cut(s) 322
DpnII GATC 1 cut(s) 320
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 298
EcoICRI GAGCTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 3 cut(s) 97, 467, 497
FaiI YATR 9 cut(s) 95, 125, 127, 166, 207, 239, 440, 465, 495
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FaqI GGGAC 2 cut(s) 367, 414
FatI CATG 3 cut(s) 93, 463, 493
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 3 cut(s) 63, 219, 414
FokI GGATG 1 cut(s) 305
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 63, 219, 414
GluI GCNGC 3 cut(s) 63, 219, 414
GsuI CTGGAG 1 cut(s) 270
HapII CCGG 1 cut(s) 108
HgaI GACGC 1 cut(s) 341
Hin1II CATG 3 cut(s) 97, 467, 497
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 1 cut(s) 158
HpaII CCGG 1 cut(s) 108
HphI GGTGA 3 cut(s) 30, 92, 167
Hpy166II GTNNAC 2 cut(s) 313, 331
Hpy188I TCNGA 1 cut(s) 51
Hpy188III TCNNGA 6 cut(s) 80, 278, 287, 446, 458, 500
Hpy8I GTNNAC 2 cut(s) 313, 331
HpyAV CCTTC 1 cut(s) 311
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 4 cut(s) 65, 97, 134, 218
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 227, 473, 494
HpyF3I CTNAG 5 cut(s) 42, 58, 143, 263, 324
Hsp92II CATG 3 cut(s) 97, 467, 497
Kzo9I GATC 1 cut(s) 320
LmnI GCTCC 2 cut(s) 303, 460
LpnPI CCDG 7 cut(s) 121, 263, 300, 311, 343, 370, 443
Lsp1109I GCAGC 3 cut(s) 49, 230, 425
LweI GCATC 1 cut(s) 106
MaeIII GTNAC 1 cut(s) 36
MalI GATC 1 cut(s) 322
MboI GATC 1 cut(s) 320
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 3 cut(s) 13, 86, 393
MnlI CCTC 9 cut(s) 51, 145, 178, 283, 442, 445, 478, 496, 499
Mph1103I ATGCAT 1 cut(s) 99
MseI TTAA 2 cut(s) 21, 89
MspA1I CMGCKG 1 cut(s) 62
MspCI CTTAAG 1 cut(s) 20
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 358
MvaI CCWGG 1 cut(s) 358
MwoI GCNNNNNNNGC 4 cut(s) 71, 227, 473, 494
NdeII GATC 1 cut(s) 320
NlaIII CATG 3 cut(s) 97, 467, 497
NlaIV GGNNCC 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 99
NspI RCATGY 2 cut(s) 467, 497
PaeI GCATGC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 158
Pfl23II CGTACG 1 cut(s) 192
PkrI GCNGC 3 cut(s) 64, 220, 415
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 356
PspLI CGTACG 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 355
PspPI GGNCC 1 cut(s) 354
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 115, 194
RsaNI GTAC 2 cut(s) 114, 193
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 2 cut(s) 21, 89
SatI GCNGC 3 cut(s) 63, 219, 414
Sau3AI GATC 1 cut(s) 320
Sau96I GGNCC 1 cut(s) 354
ScrFI CCNGG 1 cut(s) 358
SduI GDGCHC 1 cut(s) 300
SetI ASST 8 cut(s) 43, 64, 121, 171, 189, 300, 330, 456
SfaNI GCATC 1 cut(s) 106
SinI GGWCC 1 cut(s) 354
SmlI CTYRAG 3 cut(s) 20, 446, 498
SmoI CTYRAG 3 cut(s) 20, 446, 498
SphI GCATGC 1 cut(s) 467
Sse9I AATT 3 cut(s) 13, 86, 393
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 356
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 79, 319
TasI AATT 3 cut(s) 13, 86, 393
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 2 cut(s) 21, 89
Tru9I TTAA 2 cut(s) 21, 89
TseFI GTSAC 1 cut(s) 36
TseI GCWGC 3 cut(s) 62, 218, 413
Tsp45I GTSAC 1 cut(s) 36
TspDTI ATGAA 1 cut(s) 112
Vha464I CTTAAG 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 354
XceI RCATGY 2 cut(s) 467, 497
XmiI GTMKAC 1 cut(s) 312
Zsp2I ATGCAT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.