FvH4_4g14460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000776
Physical Location & Seq
Reverse (-)
18144349 .. 18146862
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14460.t1

Sequence Viewer

Length: 999 bp
ATGCCGGTGCTCGGGAAAAGGAAAGCTCGGGAGGAGGCGGAGGAGCGTTTGCGACTGGAGTGTGCGCAACAATACGGCTCCTCTTCTGCCGGATCCAACACAGCCACTCCGACGACGACGGCGCCATCCCTTGCTGAATCGGCTCCCTCTCTAACCGACGACGATGACGAGTCAGTGACCCGAGTATCAGAAACACCGTCTGAGGGTGCACCCCGGGTTGTCAGCGCTCGCCGGAAACCGAAGAAAAGCGGAGGACCTCCCCAGAAAGGCAACCGGGGTGTAGTGAACTGCTCAAAGGTGTCTGATATTGTGAAAAGCACGGGGAAGAAGATTGACTTGGACTTTGATTGGGAGTTTATTGATGGACCTCGTAATTCTGATGTATGCAGCCTTTTTGTCAATACTGTTGGAAGCATCATACGCAAAAGAGTGGCAATGAAGAAGGAATCGTGGTATGATTTAGACAAGGAGGAAAGGAAGAAGCTTTTTGACGGTCTCACTGAAAAGTTGAAGGAACAAGGTGCTATTGCTAAGACTTTGCAAAGCACTCAACATCCGGACATCCCACCGGATCAGCTGCCTCCTTTGGACTGGCCAACTCAACTAAGTGTTGTGCAAAATGCTATGGGAGAAACAAAAGGGTCTTACATTCGTGGTATTGGACGAGTAGGCGTCCTAGAGAAGAGCAGAAGGTCATCATCTTCCTCTTCATGCTCTCGACCGAATAATGAGACGATGGCTGAGATAGTGGCTGTTAGAAAGGAGGCGGCTGAGGCACAAGAGAGGGCTGACAAAGCACAAGAGAGGGCTGAAAATGCCCAAAAGATCGCTGAGCAGTCTGTTGCAGATTATCAACTGTTGAAGTTGCAAATGGAGCAAATCATGGCTCGAATAGGGCTACAGCCTATGTGTTCACCCTCGGTCCCAAGCCAACAACCTCGAGACGACGATGATGATAGGGGCCTTGACACTTCTCAGATTAGTTTAGGAAATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

333

Amino Acids

36.39

Weight (kDa)

6.14

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 66
AccB1I GGYRCC 1 cut(s) 121
AccIII TCCGGA 1 cut(s) 556
AciI CCGC 3 cut(s) 38, 249, 767
AclWI GGATC 3 cut(s) 87, 100, 579
AcoI YGGCCR 1 cut(s) 593
AcyI GRCGYC 2 cut(s) 122, 672
AfeI AGCGCT 1 cut(s) 226
AfiI CCNNNNNNNGG 3 cut(s) 11, 203, 266
AgsI TTSAA 2 cut(s) 511, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 3 cut(s) 26, 484, 577
AluI AGCT 3 cut(s) 26, 484, 577
Alw21I GWGCWC 2 cut(s) 12, 211
Alw26I GTCTC 3 cut(s) 500, 725, 936
Alw44I GTGCAC 1 cut(s) 207
AlwI GGATC 3 cut(s) 87, 100, 579
Ama87I CYCGRG 5 cut(s) 11, 27, 180, 213, 939
Aor13HI TCCGGA 1 cut(s) 556
Aor51HI AGCGCT 1 cut(s) 226
AoxI GGCC 2 cut(s) 593, 961
ApaLI GTGCAC 1 cut(s) 207
ApeKI GCWGC 2 cut(s) 387, 577
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspLEI GCGC 3 cut(s) 67, 124, 227
AspS9I GGNCC 4 cut(s) 254, 365, 922, 961
AsuC2I CCSGG 3 cut(s) 214, 215, 275
AsuHPI GGTGA 1 cut(s) 906
AvaI CYCGRG 5 cut(s) 11, 27, 180, 213, 939
AvaII GGWCC 3 cut(s) 254, 365, 922
BaeGI GKGCMC 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 168, 201
BalI TGGCCA 1 cut(s) 595
BamHI GGATCC 1 cut(s) 92
BanI GGYRCC 1 cut(s) 121
Bbv12I GWGCWC 2 cut(s) 12, 211
BbvCI CCTCAGC 1 cut(s) 771
BbvI GCAGC 2 cut(s) 399, 564
BccI CCATC 3 cut(s) 133, 356, 730
BceAI ACGGC 2 cut(s) 91, 135
BcnI CCSGG 3 cut(s) 214, 215, 275
BcoDI GTCTC 3 cut(s) 500, 725, 936
BfaI CTAG 1 cut(s) 677
BfmI CTRYAG 1 cut(s) 899
BfoI RGCGCY 2 cut(s) 125, 228
BisI GCNGC 3 cut(s) 388, 578, 768
BlpI GCTNAGC 1 cut(s) 831
BlsI GCNGC 3 cut(s) 389, 579, 769
Bme1390I CCNGG 3 cut(s) 214, 215, 275
Bme18I GGWCC 3 cut(s) 254, 365, 922
BmeT110I CYCGRG 5 cut(s) 11, 27, 180, 213, 939
BmgT120I GGNCC 4 cut(s) 254, 365, 922, 961
BmiI GGNNCC 6 cut(s) 79, 94, 123, 144, 924, 962
BmrFI CCNGG 3 cut(s) 214, 215, 275
BmsI GCATC 1 cut(s) 423
BpmI CTGGAG 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 771
Bpu1102I GCTNAGC 1 cut(s) 831
BpuMI CCSGG 3 cut(s) 214, 215, 275
BsaHI GRCGYC 2 cut(s) 122, 672
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 4 cut(s) 212, 213, 274, 918
BsaWI WCCGGW 2 cut(s) 556, 568
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 203, 266
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 60, 596
Bse3DI GCAATG 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 556
BseDI CCNNGG 4 cut(s) 212, 213, 274, 918
BseGI GGATG 3 cut(s) 125, 553, 561
BseLI CCNNNNNNNGG 3 cut(s) 11, 203, 266
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 5 cut(s) 192, 732, 762, 822, 989
BseNI ACTGG 2 cut(s) 60, 596
BseRI GAGGAG 3 cut(s) 47, 56, 70
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 2 cut(s) 399, 564
Bsh1285I CGRYCG 1 cut(s) 722
BshFI GGCC 2 cut(s) 595, 963
BshNI GGYRCC 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 722
BsiHKAI GWGCWC 2 cut(s) 12, 211
BsiHKCI CYCGRG 5 cut(s) 11, 27, 180, 213, 939
BsiSI CCGG 7 cut(s) 5, 90, 214, 232, 274, 557, 569
BslFI GGGAC 1 cut(s) 908
BslI CCNNNNNNNGG 3 cut(s) 11, 203, 266
BsmAI GTCTC 3 cut(s) 500, 725, 936
BsmBI CGTCTC 2 cut(s) 725, 936
BsmFI GGGAC 1 cut(s) 908
BsnI GGCC 2 cut(s) 595, 963
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 5 cut(s) 11, 27, 180, 213, 939
Bsp1286I GDGCHC 2 cut(s) 12, 211
Bsp13I TCCGGA 1 cut(s) 556
Bsp143I GATC 3 cut(s) 92, 571, 825
Bsp1720I GCTNAGC 1 cut(s) 831
BspACI CCGC 3 cut(s) 38, 249, 767
BspANI GGCC 2 cut(s) 595, 963
BspCNI CTCAG 5 cut(s) 193, 733, 763, 823, 988
BspEI TCCGGA 1 cut(s) 556
BspLI GGNNCC 6 cut(s) 79, 94, 123, 144, 924, 962
BspPI GGATC 3 cut(s) 87, 100, 579
BspQI GCTCTTC 1 cut(s) 677
BspT107I GGYRCC 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 2 cut(s) 60, 596
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 4 cut(s) 212, 213, 274, 918
BssMI GATC 3 cut(s) 92, 571, 825
BssNI GRCGYC 2 cut(s) 122, 672
Bst4CI ACNGT 4 cut(s) 198, 406, 494, 858
Bst6I CTCTTC 3 cut(s) 88, 677, 712
BstACI GRCGYC 2 cut(s) 122, 672
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 7 cut(s) 201, 531, 605, 741, 771, 831, 975
BstF5I GGATG 3 cut(s) 125, 553, 561
BstH2I RGCGCY 2 cut(s) 125, 228
BstHHI GCGC 3 cut(s) 67, 124, 227
BstKTI GATC 3 cut(s) 95, 574, 828
BstMAI GTCTC 3 cut(s) 500, 725, 936
BstMBI GATC 3 cut(s) 92, 571, 825
BstMCI CGRYCG 1 cut(s) 722
BstMWI GCNNNNNNNGC 6 cut(s) 140, 420, 773, 794, 815, 874
BstSCI CCNGG 3 cut(s) 212, 213, 273
BstSFI CTRYAG 1 cut(s) 899
BstSLI GKGCMC 1 cut(s) 211
BstV1I GCAGC 2 cut(s) 399, 564
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 2 cut(s) 595, 963
BtsCI GGATG 3 cut(s) 125, 553, 561
BtsIMutI CAGTG 2 cut(s) 180, 498
Cac8I GCNNGC 1 cut(s) 229
CfoI GCGC 3 cut(s) 67, 124, 227
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 4 cut(s) 254, 365, 922, 961
Cfr9I CCCGGG 1 cut(s) 213
CseI GACGC 1 cut(s) 661
CviAII CATG 2 cut(s) 711, 883
DdeI CTNAG 7 cut(s) 201, 531, 605, 741, 771, 831, 975
DinI GGCGCC 1 cut(s) 123
DpnI GATC 3 cut(s) 94, 573, 827
DpnII GATC 3 cut(s) 92, 571, 825
EaeI YGGCCR 1 cut(s) 593
Eam1104I CTCTTC 3 cut(s) 88, 677, 712
EarI CTCTTC 3 cut(s) 88, 677, 712
EciI GGCGGA 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 3 cut(s) 254, 365, 922
Eco47III AGCGCT 1 cut(s) 226
Eco88I CYCGRG 5 cut(s) 11, 27, 180, 213, 939
EcoO109I RGGNCCY 2 cut(s) 254, 961
EgeI GGCGCC 1 cut(s) 123
EheI GGCGCC 1 cut(s) 123
Esp3I CGTCTC 2 cut(s) 725, 936
FaeI CATG 2 cut(s) 714, 886
FaiI YATR 7 cut(s) 385, 419, 456, 626, 712, 884, 908
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 1 cut(s) 908
FatI CATG 2 cut(s) 710, 882
Fnu4HI GCNGC 3 cut(s) 388, 578, 768
FokI GGATG 3 cut(s) 112, 540, 548
Fsp4HI GCNGC 3 cut(s) 388, 578, 768
FspBI CTAG 1 cut(s) 677
FspI TGCGCA 1 cut(s) 66
GlaI GCGC 3 cut(s) 66, 123, 226
GluI GCNGC 3 cut(s) 388, 578, 768
GsuI CTGGAG 1 cut(s) 77
HaeII RGCGCY 2 cut(s) 125, 228
HaeIII GGCC 2 cut(s) 595, 963
HapII CCGG 7 cut(s) 5, 90, 214, 232, 274, 557, 569
HgaI GACGC 1 cut(s) 661
HhaI GCGC 3 cut(s) 67, 124, 227
Hin1I GRCGYC 2 cut(s) 122, 672
Hin1II CATG 2 cut(s) 714, 886
Hin6I GCGC 3 cut(s) 65, 122, 225
HinP1I GCGC 3 cut(s) 65, 122, 225
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 3 cut(s) 137, 170, 446
HpaII CCGG 7 cut(s) 5, 90, 214, 232, 274, 557, 569
HphI GGTGA 1 cut(s) 906
Hpy166II GTNNAC 3 cut(s) 209, 286, 914
Hpy188I TCNGA 6 cut(s) 111, 190, 202, 304, 379, 978
Hpy188III TCNNGA 5 cut(s) 13, 29, 557, 717, 941
Hpy8I GTNNAC 3 cut(s) 209, 286, 914
Hpy99I CGWCG 6 cut(s) 115, 118, 121, 161, 164, 950
HpyAV CCTTC 3 cut(s) 436, 505, 684
HpyCH4III ACNGT 4 cut(s) 198, 406, 494, 858
HpyCH4V TGCA 6 cut(s) 209, 387, 541, 616, 845, 868
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 420, 773, 794, 815, 874
HpyF3I CTNAG 7 cut(s) 201, 531, 605, 741, 771, 831, 975
Hsp92I GRCGYC 2 cut(s) 122, 672
Hsp92II CATG 2 cut(s) 714, 886
HspAI GCGC 3 cut(s) 65, 122, 225
KasI GGCGCC 1 cut(s) 121
Kpn2I TCCGGA 1 cut(s) 556
Kzo9I GATC 3 cut(s) 92, 571, 825
LguI GCTCTTC 1 cut(s) 677
LmnI GCTCC 4 cut(s) 43, 83, 148, 874
Lsp1109I GCAGC 2 cut(s) 399, 564
LweI GCATC 1 cut(s) 423
MaeI CTAG 1 cut(s) 677
MaeIII GTNAC 1 cut(s) 175
MalI GATC 3 cut(s) 94, 573, 827
MboI GATC 3 cut(s) 92, 571, 825
MboII GAAGA 9 cut(s) 75, 253, 337, 340, 451, 490, 693, 694, 699
MflI RGATCY 1 cut(s) 92
MhlI GDGCHC 2 cut(s) 12, 211
MlsI TGGCCA 1 cut(s) 595
MluCI AATT 1 cut(s) 373
MluNI TGGCCA 1 cut(s) 595
Mly113I GGCGCC 1 cut(s) 122
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 3 cut(s) 120, 134, 388
Mox20I TGGCCA 1 cut(s) 595
MroI TCCGGA 1 cut(s) 556
MscI TGGCCA 1 cut(s) 595
Msp20I TGGCCA 1 cut(s) 595
MspA1I CMGCKG 1 cut(s) 577
MspI CCGG 7 cut(s) 5, 90, 214, 232, 274, 557, 569
MspR9I CCNGG 3 cut(s) 214, 215, 275
MwoI GCNNNNNNNGC 6 cut(s) 140, 420, 773, 794, 815, 874
NarI GGCGCC 1 cut(s) 122
NciI CCSGG 3 cut(s) 214, 215, 275
NdeII GATC 3 cut(s) 92, 571, 825
NlaIII CATG 2 cut(s) 714, 886
NlaIV GGNNCC 6 cut(s) 79, 94, 123, 144, 924, 962
NmuCI GTSAC 1 cut(s) 175
NsbI TGCGCA 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 939
PciSI GCTCTTC 1 cut(s) 677
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfeI GAWTC 2 cut(s) 137, 446
PkrI GCNGC 3 cut(s) 389, 579, 769
PleI GAGTC 1 cut(s) 178
PluTI GGCGCC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 178
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
PspN4I GGNNCC 6 cut(s) 79, 94, 123, 144, 924, 962
PspPI GGNCC 4 cut(s) 254, 365, 922, 961
PspPPI RGGWCCY 1 cut(s) 254
PsuI RGATCY 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 677
SatI GCNGC 3 cut(s) 388, 578, 768
Sau3AI GATC 3 cut(s) 92, 571, 825
Sau96I GGNCC 4 cut(s) 254, 365, 922, 961
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 3 cut(s) 214, 215, 275
SduI GDGCHC 2 cut(s) 12, 211
SetI ASST 9 cut(s) 28, 259, 300, 370, 486, 523, 579, 695, 940
SfaNI GCATC 1 cut(s) 423
SfcI CTRYAG 1 cut(s) 899
SfoI GGCGCC 1 cut(s) 123
Sfr274I CTCGAG 1 cut(s) 939
SinI GGWCC 3 cut(s) 254, 365, 922
SlaI CTCGAG 1 cut(s) 939
SmaI CCCGGG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 939
SmoI CTYRAG 1 cut(s) 939
Sse9I AATT 1 cut(s) 373
SsiI CCGC 3 cut(s) 38, 249, 767
SspDI GGCGCC 1 cut(s) 121
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 3 cut(s) 212, 213, 273
TaaI ACNGT 4 cut(s) 198, 406, 494, 858
TaqI TCGA 3 cut(s) 718, 889, 940
TaqII GACCGA 2 cut(s) 736, 910
TasI AATT 1 cut(s) 373
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 2 cut(s) 137, 446
TscAI CASTG 2 cut(s) 180, 505
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 2 cut(s) 387, 577
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 2 cut(s) 452, 699
TspMI CCCGGG 1 cut(s) 213
TspRI CASTG 2 cut(s) 180, 505
VneI GTGCAC 1 cut(s) 207
VpaK11BI GGWCC 3 cut(s) 254, 365, 922
XhoI CTCGAG 1 cut(s) 939
XmaI CCCGGG 1 cut(s) 213
XspI CTAG 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.