FvH4_3g16141

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000319
Physical Location & Seq
Forward (+)
10172877 .. 10174896
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16141.t1

Sequence Viewer

Length: 1179 bp
ATGGATCCTTCTATGTACAGGGCAGCAACTTGTGGTGATGTTGGCATCTTGCAAAAGATTAGAAATGGTGAGGTATCAGTTGATCTTCTCAGTTATCAAACCCCTAAAGGTAACAATGTTCTTCATCTTGCAGCTGAATTCAAGCGTATCAATGTCTTCACAGACATCCCTCTTGACAAACATTCACCGTTGTTCTGGTCCGTCAACAAGATGGGTGATACCCCCTTACACATTGCTGCCAGAGTAGGATGTGACGAAGTGATAGACTTCCTCATCGAACGTGCAAAAGCCCTACATGTTCGAAGAGCCAACGATCAGGAAAGGGGACCAACTTATGCTAAATCTTACAAAAAGTTGCTACGAAAGACCAACTTGAAATGCATACTCGCTAAGGAGTCACCATTGTTCTTGGCAATCTGTAAGGGCTTTTCAAGTATTGCTCGTGACATTTTGAATGAGTCTTCAATATCTCCTTCTTTCCAAGGAATTGATGCATTTAGAGGGAACCTTGAAGCTACTCGGTTGTTGATGAAATGGGATAAGTCTAGTACTTCTTACATTTTGGATAAATCTGGAATGTCAGCTCTTCATGTCGCAGCATATAAAGTTTTAGGTGCACAAAGCAATGTTGTCGAGTACATAGTGAAGACCCCTGAGCTAGCAGGACTTGTAGATCAAGTCGATAAGGAAGGAAACACTCCTTTGCATTTAGCTGTCACTTGCCAAGATCATGAAATTATAAGAATTCTCACGGTTGATTGTGGAGTGCAAAGTCCTACCATCAAGGAGAGCCTATTCAACGCACCCGGGAGAAAGAGGCGACGCAGGGAAGGAGAGGGAGGCCCTGGAATACCTTGCCGGAGACTCTCCGTGAAAATTTCCAGAAAAGAGGTATTAAGGATTCGGGCTCAAGGAAAAAAAGACAAAGGAATTGAACAAGAAGAGAAGAGAAGAGGAGAGAAACCCTACCCACCGCTGTTACAAGTAGAAGAAGAGAAAGAGTTTGGTGGTGGTCTCGCCGTCTGGGGTGGCCGGAATCGGGCGGAGGGGGGCGAAGAAGATGGATGCGGCGGAGAGAGAGGAGGAGGAGAGAGACAGACGGGTCAACGCCCGAAATGGGCTTTTGACCGAGGTCAACAGTCTCTTCCCTTTTATAGGGAAATGCACGGCCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

393

Amino Acids

43.64

Weight (kDa)

9.18

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 8 - 94 4.5e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 165 - 253 3.7e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 740
AasI GACNNNNNNGTC 1 cut(s) 1101
AciI CCGC 4 cut(s) 974, 1043, 1068, 1071
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 1030, 1168
AcsI RAATTY 3 cut(s) 137, 744, 876
AfaI GTAC 3 cut(s) 17, 550, 638
AfiI CCNNNNNNNGG 3 cut(s) 1039, 1117, 1155
AflIII ACRYGT 1 cut(s) 295
AgsI TTSAA 8 cut(s) 142, 376, 432, 454, 465, 512, 799, 935
AjnI CCWGG 1 cut(s) 844
AluBI AGCT 5 cut(s) 134, 515, 584, 658, 713
AluI AGCT 5 cut(s) 134, 515, 584, 658, 713
Alw21I GWGCWC 1 cut(s) 619
Alw26I GTCTC 4 cut(s) 856, 1019, 1087, 1146
Alw44I GTGCAC 1 cut(s) 615
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 806
AoxI GGCC 3 cut(s) 841, 1030, 1168
ApaLI GTGCAC 1 cut(s) 615
ApeKI GCWGC 4 cut(s) 23, 131, 236, 596
ApoI RAATTY 3 cut(s) 137, 744, 876
AspS9I GGNCC 3 cut(s) 198, 326, 842
AsuC2I CCSGG 2 cut(s) 807, 808
AsuHPI GGTGA 5 cut(s) 47, 80, 177, 227, 390
AsuII TTCGAA 1 cut(s) 301
AsuNHI GCTAGC 1 cut(s) 658
AvaI CYCGRG 1 cut(s) 806
AvaII GGWCC 2 cut(s) 198, 326
BaeGI GKGCMC 1 cut(s) 619
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 910
BauI CACGAG 1 cut(s) 441
BbsI GAAGAC 3 cut(s) 148, 453, 653
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 4 cut(s) 35, 143, 223, 608
BccI CCATC 3 cut(s) 205, 788, 1055
BceAI ACGGC 1 cut(s) 1004
BcgI CGANNNNNNTGC 2 cut(s) 613, 647
BciT130I CCWGG 1 cut(s) 846
BcnI CCSGG 2 cut(s) 807, 808
BcoDI GTCTC 4 cut(s) 856, 1019, 1087, 1146
BfaI CTAG 2 cut(s) 546, 659
BisI GCNGC 5 cut(s) 24, 132, 237, 597, 1069
BlsI GCNGC 5 cut(s) 25, 133, 238, 598, 1070
BmcAI AGTACT 1 cut(s) 550
Bme1390I CCNGG 3 cut(s) 807, 808, 846
Bme18I GGWCC 2 cut(s) 198, 326
BmeT110I CYCGRG 1 cut(s) 806
BmgT120I GGNCC 3 cut(s) 198, 326, 842
BmiI GGNNCC 3 cut(s) 6, 327, 506
BmrFI CCNGG 3 cut(s) 807, 808, 846
BmsI GCATC 3 cut(s) 54, 481, 1055
BmtI GCTAGC 1 cut(s) 662
BoxI GACNNNNGTC 1 cut(s) 1131
BpiI GAAGAC 3 cut(s) 148, 453, 653
Bpu10I CCTNAGC 2 cut(s) 390, 654
Bpu14I TTCGAA 1 cut(s) 301
BpuEI CTTGAG 1 cut(s) 894
BpuMI CCSGG 2 cut(s) 807, 808
BsaI GGTCTC 1 cut(s) 1019
BsaJI CCNNGG 4 cut(s) 481, 806, 844, 1129
Bsc4I CCNNNNNNNGG 3 cut(s) 1039, 1117, 1155
Bse3DI GCAATG 2 cut(s) 231, 631
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 4 cut(s) 481, 806, 844, 1129
BseGI GGATG 3 cut(s) 165, 254, 1070
BseLI CCNNNNNNNGG 3 cut(s) 1039, 1117, 1155
BseMI GCAATG 2 cut(s) 231, 631
BseMII CTCAG 2 cut(s) 103, 645
BseRI GAGGAG 4 cut(s) 969, 1095, 1098, 1101
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 4 cut(s) 35, 143, 223, 608
BshFI GGCC 3 cut(s) 843, 1032, 1170
BsiHKAI GWGCWC 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 806
BsiSI CCGG 3 cut(s) 807, 859, 1033
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 3 cut(s) 1039, 1117, 1155
BsmAI GTCTC 4 cut(s) 856, 1019, 1087, 1146
BsmFI GGGAC 1 cut(s) 339
BsnI GGCC 3 cut(s) 843, 1032, 1170
Bso31I GGTCTC 1 cut(s) 1019
BsoBI CYCGRG 1 cut(s) 806
Bsp119I TTCGAA 1 cut(s) 301
Bsp1286I GDGCHC 2 cut(s) 619, 910
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 5 cut(s) 4, 82, 313, 673, 727
BspACI CCGC 4 cut(s) 974, 1043, 1068, 1071
BspANI GGCC 3 cut(s) 843, 1032, 1170
BspCNI CTCAG 2 cut(s) 102, 646
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 3 cut(s) 6, 327, 506
BspOI GCTAGC 1 cut(s) 662
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 2 cut(s) 298, 591
BspT104I TTCGAA 1 cut(s) 301
BspTNI GGTCTC 1 cut(s) 1019
BsrDI GCAATG 2 cut(s) 231, 631
BsrGI TGTACA 1 cut(s) 15
BssECI CCNNGG 4 cut(s) 481, 806, 844, 1129
BssMI GATC 5 cut(s) 4, 82, 313, 673, 727
BssSI CACGAG 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 481
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 846
Bst4CI ACNGT 3 cut(s) 189, 754, 1140
Bst6I CTCTTC 7 cut(s) 298, 591, 936, 941, 946, 987, 1149
BstAUI TGTACA 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 660
BstDEI CTNAG 3 cut(s) 89, 390, 654
BstENI CCTNNNNNAGG 1 cut(s) 1153
BstF5I GGATG 3 cut(s) 165, 254, 1070
BstKTI GATC 5 cut(s) 7, 85, 316, 676, 730
BstMAI GTCTC 4 cut(s) 856, 1019, 1087, 1146
BstMBI GATC 5 cut(s) 4, 82, 313, 673, 727
BstNI CCWGG 1 cut(s) 846
BstNSI RCATGY 1 cut(s) 299
BstPAI GACNNNNGTC 1 cut(s) 1131
BstSCI CCNGG 3 cut(s) 805, 806, 844
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 4 cut(s) 35, 143, 223, 608
BstV2I GAAGAC 3 cut(s) 148, 453, 653
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 3 cut(s) 843, 1032, 1170
BtsCI GGATG 3 cut(s) 165, 254, 1070
Cac8I GCNNGC 1 cut(s) 660
CciI TCATGA 1 cut(s) 730
Cfr13I GGNCC 3 cut(s) 198, 326, 842
Cfr9I CCCGGG 1 cut(s) 806
CseI GACGC 1 cut(s) 831
Csp6I GTAC 3 cut(s) 16, 549, 637
CviAII CATG 3 cut(s) 296, 590, 731
CviQI GTAC 3 cut(s) 16, 549, 637
DdeI CTNAG 3 cut(s) 89, 390, 654
DpnI GATC 5 cut(s) 6, 84, 315, 675, 729
DpnII GATC 5 cut(s) 4, 82, 313, 673, 727
DrdI GACNNNNNNGTC 1 cut(s) 1101
DseDI GACNNNNNNGTC 1 cut(s) 1101
EaeI YGGCCR 2 cut(s) 1030, 1168
Eam1104I CTCTTC 7 cut(s) 298, 591, 936, 941, 946, 987, 1149
EarI CTCTTC 7 cut(s) 298, 591, 936, 941, 946, 987, 1149
EciI GGCGGA 2 cut(s) 1058, 1086
Eco130I CCWWGG 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 910
Eco31I GGTCTC 1 cut(s) 1019
Eco47I GGWCC 2 cut(s) 198, 326
Eco88I CYCGRG 1 cut(s) 806
EcoNI CCTNNNNNAGG 1 cut(s) 1153
EcoO109I RGGNCCY 1 cut(s) 842
EcoRI GAATTC 2 cut(s) 137, 744
EcoRII CCWGG 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 481
EcoT22I ATGCAT 2 cut(s) 383, 496
EcoT38I GRGCYC 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 481
FaeI CATG 3 cut(s) 299, 593, 734
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 339
FatI CATG 3 cut(s) 295, 589, 730
Fnu4HI GCNGC 5 cut(s) 24, 132, 237, 597, 1069
FokI GGATG 3 cut(s) 152, 261, 1077
FriOI GRGCYC 1 cut(s) 910
Fsp4HI GCNGC 5 cut(s) 24, 132, 237, 597, 1069
FspBI CTAG 2 cut(s) 546, 659
GluI GCNGC 5 cut(s) 24, 132, 237, 597, 1069
HaeIII GGCC 3 cut(s) 843, 1032, 1170
HapII CCGG 3 cut(s) 807, 859, 1033
HgaI GACGC 1 cut(s) 831
Hin1II CATG 3 cut(s) 299, 593, 734
HincII GTYRAC 3 cut(s) 205, 1106, 1136
HindII GTYRAC 3 cut(s) 205, 1106, 1136
HinfI GANTC 5 cut(s) 395, 458, 864, 901, 1036
HpaII CCGG 3 cut(s) 807, 859, 1033
HphI GGTGA 5 cut(s) 47, 80, 177, 227, 390
Hpy166II GTNNAC 4 cut(s) 205, 617, 1106, 1136
Hpy188III TCNNGA 6 cut(s) 173, 317, 443, 573, 731, 882
Hpy8I GTNNAC 4 cut(s) 205, 617, 1106, 1136
Hpy99I CGWCG 1 cut(s) 825
HpyAV CCTTC 4 cut(s) 18, 483, 683, 824
HpyCH4III ACNGT 3 cut(s) 189, 754, 1140
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 9 cut(s) 52, 131, 284, 381, 494, 617, 706, 769, 1165
HpyF3I CTNAG 3 cut(s) 89, 390, 654
HpySE526I ACGT 1 cut(s) 280
Hsp92II CATG 3 cut(s) 299, 593, 734
Kzo9I GATC 5 cut(s) 4, 82, 313, 673, 727
LguI GCTCTTC 2 cut(s) 298, 591
Lsp1109I GCAGC 4 cut(s) 35, 143, 223, 608
LweI GCATC 3 cut(s) 54, 481, 1055
MaeI CTAG 2 cut(s) 546, 659
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 6 cut(s) 110, 251, 396, 443, 715, 978
MalI GATC 5 cut(s) 6, 84, 315, 675, 729
MboI GATC 5 cut(s) 4, 82, 313, 673, 727
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 619, 910
MluCI AATT 6 cut(s) 137, 486, 735, 744, 876, 930
MlyI GAGTC 3 cut(s) 404, 467, 858
Mph1103I ATGCAT 2 cut(s) 383, 496
MseI TTAA 2 cut(s) 896, 1177
MspA1I CMGCKG 2 cut(s) 134, 976
MspI CCGG 3 cut(s) 807, 859, 1033
MspR9I CCNGG 3 cut(s) 807, 808, 846
MvaI CCWGG 1 cut(s) 846
NciI CCSGG 2 cut(s) 807, 808
NdeII GATC 5 cut(s) 4, 82, 313, 673, 727
NheI GCTAGC 1 cut(s) 658
NlaIII CATG 3 cut(s) 299, 593, 734
NlaIV GGNNCC 3 cut(s) 6, 327, 506
NmuCI GTSAC 4 cut(s) 251, 396, 443, 715
NsiI ATGCAT 2 cut(s) 383, 496
NspI RCATGY 1 cut(s) 299
NspV TTCGAA 1 cut(s) 301
PagI TCATGA 1 cut(s) 730
PciI ACATGT 1 cut(s) 295
PciSI GCTCTTC 2 cut(s) 298, 591
PfeI GAWTC 2 cut(s) 901, 1036
PkrI GCNGC 5 cut(s) 25, 133, 238, 598, 1070
PleI GAGTC 3 cut(s) 403, 466, 858
PpsI GAGTC 3 cut(s) 403, 466, 858
PscI ACATGT 1 cut(s) 295
PshAI GACNNNNGTC 1 cut(s) 1131
PsiI TTATAA 1 cut(s) 740
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspN4I GGNNCC 3 cut(s) 6, 327, 506
PspPI GGNCC 3 cut(s) 198, 326, 842
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 3 cut(s) 17, 550, 638
RsaNI GTAC 3 cut(s) 16, 549, 637
SapI GCTCTTC 2 cut(s) 298, 591
SaqAI TTAA 2 cut(s) 896, 1177
SatI GCNGC 5 cut(s) 24, 132, 237, 597, 1069
Sau3AI GATC 5 cut(s) 4, 82, 313, 673, 727
Sau96I GGNCC 3 cut(s) 198, 326, 842
ScaI AGTACT 1 cut(s) 550
SchI GAGTC 3 cut(s) 404, 467, 858
ScrFI CCNGG 3 cut(s) 807, 808, 846
SduI GDGCHC 2 cut(s) 619, 910
SfaNI GCATC 3 cut(s) 54, 481, 1055
SfuI TTCGAA 1 cut(s) 301
SinI GGWCC 2 cut(s) 198, 326
SmaI CCCGGG 1 cut(s) 808
SmlI CTYRAG 1 cut(s) 909
SmoI CTYRAG 1 cut(s) 909
Sse9I AATT 6 cut(s) 137, 486, 735, 744, 876, 930
SsiI CCGC 4 cut(s) 974, 1043, 1068, 1071
SspMI CTAG 2 cut(s) 546, 659
StyD4I CCNGG 3 cut(s) 805, 806, 844
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 3 cut(s) 189, 754, 1140
TaiI ACGT 1 cut(s) 283
TaqI TCGA 4 cut(s) 276, 301, 633, 681
TaqII GACCGA 1 cut(s) 1143
TasI AATT 6 cut(s) 137, 486, 735, 744, 876, 930
TatI WGTACW 3 cut(s) 15, 548, 636
TauI GCSGC 1 cut(s) 1071
TfiI GAWTC 2 cut(s) 901, 1036
Tru1I TTAA 2 cut(s) 896, 1177
Tru9I TTAA 2 cut(s) 896, 1177
TseFI GTSAC 4 cut(s) 251, 396, 443, 715
TseI GCWGC 4 cut(s) 23, 131, 236, 596
Tsp45I GTSAC 4 cut(s) 251, 396, 443, 715
TspDTI ATGAA 4 cut(s) 113, 545, 578, 747
TspGWI ACGGA 2 cut(s) 190, 859
TspMI CCCGGG 1 cut(s) 806
VneI GTGCAC 1 cut(s) 615
VpaK11BI GGWCC 2 cut(s) 198, 326
XagI CCTNNNNNAGG 1 cut(s) 1153
XapI RAATTY 3 cut(s) 137, 744, 876
XceI RCATGY 1 cut(s) 299
XmaI CCCGGG 1 cut(s) 806
XspI CTAG 2 cut(s) 546, 659
ZrmI AGTACT 1 cut(s) 550
Zsp2I ATGCAT 2 cut(s) 383, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.