MD04G1020900.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000319
Physical Location & Seq
Reverse (-)
2490352 .. 2491664
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1020900.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGCAGCAAGATAGTTCTATTTCTTACATCTTGGACAAATCTGGAATGTCAGCTCTTCATGTTGCGGCATACGCAGGCCGTCCGAAAGTGATGGAAGAGTTGACTCAATGGCGGCCTGATACTTGTGAATTGGTCAATCACAAAGGCCAAACGGTTCTGCATGCAGCAGTTTTAGGTGATCAATTTGATGTTATCAGCTACATTGTGGACACGCCTAAATATGCAGGACTTATAAATGAAGCTGACAACGACGGAAACACACCTTTGCATCTAGCTGCTAGTCGAAGTAATCCTAATGTCCTGATAGCTTTGGCAAAGAACCCTAGAGTGGATAGTACTGCTATCAATAATCAACACTTGAAGGCTGTTGACATTTTTCTTGGGGACGATATTGAACTGAGTGCCTTCAGTGGATTGCCGGTTTCGAGACTTTTGGGCCGCGCTGTTGGTGTGCCAGTCTTCCAACAACAAATCCGCCGTGAATTTATGAAACTGGAATCACCGGAAAAGAAGGATACACCAAACAGCACTTTGGTCATTGCAAAGAAGAGAGCACTTGAAGCTTATTGGGATGACTCGAACAAGTTTAACACTGATCTAGTGGTAGCGATGCTTATTGCAACCGTCACTTTTGCAGCAGCTTTCACAATGCCTGGAGGATTCAAAAGCAACGGAATGGCCGTCTTGTACAGAAGGGCATTTTTCGGAGTGTTTGTGGTATCCGACGCCATCTCCTTCTTTCTATCAATTATTGTGGTGTATAACCATTTTAGGTTGTCAGCTTTCTCACGGGTCATTCTAACGACACCTTCAAGTTTCATTCAGTACTCCATTGGAGGAATGGTGGTATCATTTTCTTCAGGTATGTTTCTAGTGCTACCCAAATACAGTCCACTTGGCATTCTGATCATTGTAGTAGCTTGCATAGTCTGCTTTGGTATCATCAGAAAGTACAGTCTTGCTGAGATACCGAACAAGCGCCAAGTGCCGTCAGGGCAATGGCTCCGATCCCGCATAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

341

Amino Acids

37.46

Weight (kDa)

9.15

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 19 - 110 4.2e-12 Ankyrin repeats (3 copies)
PGG PF13962 194 - 292 3.1e-19 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 233
AccII CGCG 1 cut(s) 441
AciI CCGC 5 cut(s) 65, 112, 439, 475, 1012
AclWI GGATC 1 cut(s) 1002
AcoI YGGCCR 1 cut(s) 678
AcsI RAATTY 1 cut(s) 482
AcuI CTGAAG 2 cut(s) 391, 843
AcyI GRCGYC 1 cut(s) 726
AfaI GTAC 4 cut(s) 337, 689, 827, 953
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 5 cut(s) 361, 395, 560, 664, 813
AjnI CCWGG 1 cut(s) 652
AluBI AGCT 9 cut(s) 53, 198, 242, 275, 308, 563, 641, 782, 920
AluI AGCT 9 cut(s) 53, 198, 242, 275, 308, 563, 641, 782, 920
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 1 cut(s) 1002
AoxI GGCC 5 cut(s) 76, 113, 145, 436, 678
ApeKI GCWGC 5 cut(s) 4, 164, 275, 635, 638
ApoI RAATTY 1 cut(s) 482
AspLEI GCGC 2 cut(s) 443, 981
AspS9I GGNCC 1 cut(s) 436
AsuHPI GGTGA 2 cut(s) 188, 492
BbsI GAAGAC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 5 cut(s) 16, 176, 262, 647, 650
BccI CCATC 2 cut(s) 85, 737
BceAI ACGGC 4 cut(s) 63, 462, 665, 973
BciT130I CCWGG 1 cut(s) 654
BciVI GTATCC 2 cut(s) 508, 730
BclI TGATCA 2 cut(s) 178, 906
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 5 cut(s) 272, 279, 324, 599, 872
BfoI RGCGCY 1 cut(s) 982
BfuI GTATCC 2 cut(s) 508, 730
BglI GCCNNNNNGGC 1 cut(s) 994
BisI GCNGC 8 cut(s) 5, 66, 113, 165, 276, 439, 636, 639
BlsI GCNGC 8 cut(s) 6, 67, 114, 166, 277, 440, 637, 640
BmcAI AGTACT 2 cut(s) 337, 827
Bme1390I CCNGG 1 cut(s) 654
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 1 cut(s) 1004
BmrFI CCNGG 1 cut(s) 654
BmsI GCATC 2 cut(s) 277, 600
BpiI GAAGAC 1 cut(s) 451
BpmI CTGGAG 1 cut(s) 675
BsaHI GRCGYC 1 cut(s) 726
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 6 cut(s) 699, 716, 729, 746, 828, 858
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 455, 498
Bse3DI GCAATG 2 cut(s) 537, 1004
BseBI CCWGG 1 cut(s) 654
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 2 cut(s) 537, 1004
BseMII CTCAG 2 cut(s) 389, 954
BseNI ACTGG 2 cut(s) 455, 498
BseXI GCAGC 5 cut(s) 16, 176, 262, 647, 650
Bsh1236I CGCG 1 cut(s) 441
BshFI GGCC 5 cut(s) 78, 115, 147, 438, 680
BsiHKAI GWGCWC 1 cut(s) 556
BsiSI CCGG 2 cut(s) 419, 503
BslFI GGGAC 1 cut(s) 398
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 398
BsmI GAATGC 1 cut(s) 900
BsnI GGCC 5 cut(s) 78, 115, 147, 438, 680
Bsp1286I GDGCHC 1 cut(s) 556
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 4 cut(s) 178, 595, 906, 1007
BspACI CCGC 5 cut(s) 65, 112, 439, 475, 1012
BspANI GGCC 5 cut(s) 78, 115, 147, 438, 680
BspCNI CTCAG 2 cut(s) 390, 955
BspFNI CGCG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 1004
BspPI GGATC 1 cut(s) 1002
BspQI GCTCTTC 1 cut(s) 60
BsrDI GCAATG 2 cut(s) 537, 1004
BsrFI RCCGGY 1 cut(s) 418
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 2 cut(s) 455, 498
BssAI RCCGGY 1 cut(s) 418
BssMI GATC 4 cut(s) 178, 595, 906, 1007
BssNI GRCGYC 1 cut(s) 726
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 4 cut(s) 154, 625, 890, 956
Bst6I CTCTTC 3 cut(s) 60, 90, 542
BstACI GRCGYC 1 cut(s) 726
BstAPI GCANNNNNTGC 1 cut(s) 930
BstAUI TGTACA 1 cut(s) 687
BstC8I GCNNGC 3 cut(s) 76, 162, 922
BstDEI CTNAG 2 cut(s) 398, 963
BstF5I GGATG 1 cut(s) 577
BstFNI CGCG 1 cut(s) 441
BstH2I RGCGCY 1 cut(s) 982
BstHHI GCGC 2 cut(s) 443, 981
BstKTI GATC 4 cut(s) 181, 598, 909, 1010
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 4 cut(s) 178, 595, 906, 1007
BstMWI GCNNNNNNNGC 5 cut(s) 71, 560, 930, 985, 994
BstNI CCWGG 1 cut(s) 654
BstNSI RCATGY 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 652
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 5 cut(s) 16, 176, 262, 647, 650
BstV2I GAAGAC 1 cut(s) 451
BsuI GTATCC 2 cut(s) 508, 730
BsuRI GGCC 5 cut(s) 78, 115, 147, 438, 680
BtgZI GCGATG 1 cut(s) 623
BtsCI GGATG 1 cut(s) 577
BtsIMutI CAGTG 2 cut(s) 415, 591
Cac8I GCNNGC 3 cut(s) 76, 162, 922
CfoI GCGC 2 cut(s) 443, 981
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 436
CseI GACGC 1 cut(s) 734
Csp6I GTAC 4 cut(s) 336, 688, 826, 952
CspCI CAANNNNNGTGG 2 cut(s) 735, 770
CviAII CATG 2 cut(s) 59, 161
CviQI GTAC 4 cut(s) 336, 688, 826, 952
DdeI CTNAG 2 cut(s) 398, 963
DpnI GATC 4 cut(s) 180, 597, 908, 1009
DpnII GATC 4 cut(s) 178, 595, 906, 1007
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 3 cut(s) 60, 90, 542
EarI CTCTTC 3 cut(s) 60, 90, 542
EciI GGCGGA 1 cut(s) 464
Eco57I CTGAAG 2 cut(s) 391, 843
EcoRII CCWGG 1 cut(s) 652
FaeI CATG 2 cut(s) 62, 164
FaqI GGGAC 1 cut(s) 398
FatI CATG 2 cut(s) 58, 160
FauI CCCGC 1 cut(s) 1019
FbaI TGATCA 2 cut(s) 178, 906
Fnu4HI GCNGC 8 cut(s) 5, 66, 113, 165, 276, 439, 636, 639
FokI GGATG 1 cut(s) 584
Fsp4HI GCNGC 8 cut(s) 5, 66, 113, 165, 276, 439, 636, 639
FspBI CTAG 5 cut(s) 272, 279, 324, 599, 872
GlaI GCGC 2 cut(s) 442, 980
GluI GCNGC 8 cut(s) 5, 66, 113, 165, 276, 439, 636, 639
GsuI CTGGAG 1 cut(s) 675
HaeII RGCGCY 1 cut(s) 982
HaeIII GGCC 5 cut(s) 78, 115, 147, 438, 680
HapII CCGG 2 cut(s) 419, 503
HgaI GACGC 1 cut(s) 734
HhaI GCGC 2 cut(s) 443, 981
Hin1I GRCGYC 1 cut(s) 726
Hin1II CATG 2 cut(s) 62, 164
Hin6I GCGC 2 cut(s) 441, 979
HinP1I GCGC 2 cut(s) 441, 979
HincII GTYRAC 2 cut(s) 102, 370
HindII GTYRAC 2 cut(s) 102, 370
HindIII AAGCTT 1 cut(s) 561
HinfI GANTC 4 cut(s) 103, 497, 575, 660
HpaII CCGG 2 cut(s) 419, 503
HphI GGTGA 2 cut(s) 188, 492
Hpy166II GTNNAC 4 cut(s) 102, 208, 370, 893
Hpy188I TCNGA 7 cut(s) 84, 707, 724, 906, 947, 1007, 1022
Hpy188III TCNNGA 3 cut(s) 42, 301, 426
Hpy8I GTNNAC 4 cut(s) 102, 208, 370, 893
Hpy99I CGWCG 2 cut(s) 254, 728
HpyAV CCTTC 6 cut(s) 355, 415, 505, 687, 745, 819
HpyCH4III ACNGT 4 cut(s) 154, 625, 890, 956
HpyCH4V TGCA 9 cut(s) 4, 160, 164, 224, 268, 542, 620, 635, 924
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 560, 930, 985, 994
HpyF3I CTNAG 2 cut(s) 398, 963
Hsp92I GRCGYC 1 cut(s) 726
Hsp92II CATG 2 cut(s) 62, 164
HspAI GCGC 2 cut(s) 441, 979
Ksp22I TGATCA 2 cut(s) 178, 906
Kzo9I GATC 4 cut(s) 178, 595, 906, 1007
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 1 cut(s) 1008
Lsp1109I GCAGC 5 cut(s) 16, 176, 262, 647, 650
LweI GCATC 2 cut(s) 277, 600
MaeI CTAG 5 cut(s) 272, 279, 324, 599, 872
MaeIII GTNAC 1 cut(s) 625
MalI GATC 4 cut(s) 180, 597, 908, 1009
MboI GATC 4 cut(s) 178, 595, 906, 1007
MboII GAAGA 5 cut(s) 47, 107, 451, 559, 849
MhlI GDGCHC 1 cut(s) 556
MluCI AATT 4 cut(s) 128, 182, 482, 747
MlyI GAGTC 2 cut(s) 97, 569
MmeI TCCRAC 2 cut(s) 487, 747
MnlI CCTC 2 cut(s) 650, 830
MseI TTAA 1 cut(s) 588
MspI CCGG 2 cut(s) 419, 503
MspR9I CCNGG 1 cut(s) 654
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 5 cut(s) 71, 560, 930, 985, 994
NdeII GATC 4 cut(s) 178, 595, 906, 1007
NlaIII CATG 2 cut(s) 62, 164
NlaIV GGNNCC 1 cut(s) 1004
NmuCI GTSAC 1 cut(s) 625
NspI RCATGY 1 cut(s) 164
PaeI GCATGC 1 cut(s) 164
PciSI GCTCTTC 1 cut(s) 60
PcsI WCGNNNNNNNCGW 1 cut(s) 678
PctI GAATGC 1 cut(s) 900
PfeI GAWTC 2 cut(s) 497, 660
PkrI GCNGC 8 cut(s) 6, 67, 114, 166, 277, 440, 637, 640
PleI GAGTC 2 cut(s) 97, 569
PpsI GAGTC 2 cut(s) 97, 569
PsiI TTATAA 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 1 cut(s) 1004
PspPI GGNCC 1 cut(s) 436
RsaI GTAC 4 cut(s) 337, 689, 827, 953
RsaNI GTAC 4 cut(s) 336, 688, 826, 952
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 8 cut(s) 5, 66, 113, 165, 276, 439, 636, 639
Sau3AI GATC 4 cut(s) 178, 595, 906, 1007
Sau96I GGNCC 1 cut(s) 436
ScaI AGTACT 2 cut(s) 337, 827
SchI GAGTC 2 cut(s) 97, 569
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 556
SfaNI GCATC 2 cut(s) 277, 600
SphI GCATGC 1 cut(s) 164
Sse9I AATT 4 cut(s) 128, 182, 482, 747
SsiI CCGC 5 cut(s) 65, 112, 439, 475, 1012
SspMI CTAG 5 cut(s) 272, 279, 324, 599, 872
StyD4I CCNGG 1 cut(s) 652
TaaI ACNGT 4 cut(s) 154, 625, 890, 956
TaqI TCGA 3 cut(s) 283, 425, 578
TasI AATT 4 cut(s) 128, 182, 482, 747
TatI WGTACW 4 cut(s) 335, 687, 825, 951
TauI GCSGC 3 cut(s) 68, 115, 441
TfiI GAWTC 2 cut(s) 497, 660
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 2 cut(s) 415, 598
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 5 cut(s) 4, 164, 275, 635, 638
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 4 cut(s) 47, 252, 503, 808
TspGWI ACGGA 2 cut(s) 267, 687
TspRI CASTG 2 cut(s) 415, 598
XapI RAATTY 1 cut(s) 482
XceI RCATGY 1 cut(s) 164
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XspI CTAG 5 cut(s) 272, 279, 324, 599, 872
ZrmI AGTACT 2 cut(s) 337, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.