Rroxscaffold_1G00052390

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000319
Physical Location & Seq
Reverse (-)
73164941 .. 73169529
4589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052390.1

Sequence Viewer

Length: 2085 bp
ATGGATCCTTGTCTATACGAGGCGGTAACATCCGGTGACGTTGATTTCTTGAAGATAAATATCAGTAATGATGACCTTCTCAGTCAGAGAACACCTAAATACAACAACATTCTTCACCTTGCTGCTGAATTCAAACACATAAACGTCTTCACGGAAGTACCACTAGATGATCAATCTCCGCTGTTTTGGGCTGCTAACAAGTATGGCGACACCCCTCTGCACGTTGCTGCAAGAGTAGGTTGCAATGAGGTAATAACATTCCTCATTGAGCACTCGAAGAGAACACTACCCGATGAAGCAGCTGATGTGGAAAGTGGACCAGCTGATGCTGAAGCCTATAAAGAGCTGCTTCGTGTTACCAATTTGAGGAAGGATACAGCATTTCATGTAGCTGTCCAATATGGTCAGAGGGAAGTGGTGAACTTGTTAATGGCGGCTGATCCCGAATTGTGCTGTTTTACTAACAGCGCCAATGAGTCACCGCTCTTCATAGCTGTTCGCAAGGACTTCAACCTCATAGCGCGTTCTATTTTAATCAAGTGTTCTGTTTCTCCTTCGTTCGCGGGAACCGATGGTGTGACAGCTTTGCATGCCGCGGTAACTCACAGGCACAAAAGCATGAGACGCCTTGTGAAGATTATGGTGTCCAAAAAAGCAGATATAATTGAAAAAACTGATGCAATTGGATGGACACCCTTGCACTACGCAGCATTGAGAGGGAACCTTAAGATAACTCGGCTACTGCTAATAGAGAATAGTTCTGTCTCTTATACTCTGGACAAGTCTGGAATGTCAGCTCTCCATATCGCGGCTTGTGCAGGTCGCACTAAAGTGTTGAAAGAGTTGATTCGATGTCGGCCTGATACTTGTGATTTACTCAATGATAAGGGCCAAAACATTCTCCATGCTGCAGTCTTAGGTCGACAAAAATTTATTATCAGGTACATGTTGAAGAATCCTAAGCTTTCCAGACTTATAAATGAAGCGGATAAAGAAGGGAACACTCCTTTACATCTAGCCGTTATTTATTACAAAGGAGACAGACTTCTGATTAGAAGTTTGACAACCGATCCTAGAGTTGATCAGACTGTTACGAATAACGAATCCTCACATGCGGTCGACATCTGTATTAGCCAGTATATTAAACAGGATATTCCAAGTCGGCATACTCACAGTTTTGGCTTGAAGAGAAATGTTGGCGTGCCAACTTTTCACAAACAAATTCTCCATGAAATCACGGCATTTGAACCCAAGGAGAACAATACGTCGAGCCCACCAACAAGTAAAGAAAATTGGCAAGCTAATAATGGAGACGAAGCTTTGAAAACCTATGACACCACTCAATTGGTAGCGGTGCTTATATCGACGGTAACATTTGCTGCAGCCTTTACCATCCCTGGAGGATTCAAAAGTGACGGCACACCGGTTTTATATGGCAAAGCATTCTTCCTAGTGTTTCTGTTATCAGACGCCTTCTCCTTCTTCACATCGATTTGTGTCGTGTTTCTTCATTTTTTGAGGGTCACTTTTGTCGACATGCCACCGCTGGGACGGTTCCTTGGAAATCTAATTTTGTATTCCCTTCTTGGAATGGTGGTAGCATTTTCGTCAGGTATTTTTATACTGATTCCAAAATCCGGTCAACCGGTTGCCATTTGTCTCCTGGTAATATGTGCCTTCCTATGCTGGTTAAATGGGGTCCAAGAACAACCGATTGCAGATGGAAGATCCGATGGCGGAGATGGTAGCCGGAGATCGAATGCGGGTTTGGGTCGAAGAGCACCGAGATTGATGTCGACACGTTCGTCGGGGAATGGAACTGTTGAACCCGGCGGAAGGCCGACGGAGCGAGACTTCGGTGGCCAACGACGAAGTGGAGATCAATTCCGAGCTCGGCCACCGATCTGCAAGCGCTCGGCGGGTAAACTTCGAAACCTTGATGGATTGAGCTCGGCCACCGATGCGCAAGCGGCTCGGCCGGTTTGTTGCAGCTCGCAACCTGGGAGTGGTAGCTCGCCGGAGCTTGGTGAATGGCGAGTGCAGATCACTCATGAGCTCGACCACCATATTTTTCAGGCCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

694

Amino Acids

76.39

Weight (kDa)

8.73

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 92 6.7e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 128 - 221 2.8e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 162 - 252 1.6e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 196 - 249 2.3e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 209 - 284 3.1e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 267 - 347 5.4e-11 Ankyrin repeats (3 copies)
PGG PF13962 442 - 541 1.3e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 977
AasI GACNNNNNNGTC 1 cut(s) 1804
Acc16I TGCGCA 1 cut(s) 1965
Acc36I ACCTGC 1 cut(s) 809
AccBSI CCGCTC 1 cut(s) 484
AccI GTMKAC 4 cut(s) 922, 1119, 1533, 1796
AccII CGCG 4 cut(s) 523, 563, 596, 809
AclWI GGATC 4 cut(s) 12, 434, 1064, 1722
AcoI YGGCCR 4 cut(s) 1861, 1895, 1953, 1976
AcsI RAATTY 3 cut(s) 128, 929, 1221
AcuI CTGAAG 1 cut(s) 351
AcyI GRCGYC 2 cut(s) 625, 1470
AfaI GTAC 2 cut(s) 159, 944
AfeI AGCGCT 1 cut(s) 1913
AfiI CCNNNNNNNGG 7 cut(s) 366, 808, 1547, 1637, 1836, 2006, 2024
AflII CTTAAG 1 cut(s) 725
AflIII ACRYGT 2 cut(s) 945, 1799
AgeI ACCGGT 2 cut(s) 1423, 1645
AjnI CCWGG 3 cut(s) 1396, 1662, 1999
AjuI GAANNNNNNNTTGG 2 cut(s) 1751, 1783
AleI CACNNNNGTG 1 cut(s) 830
Alw21I GWGCWC 5 cut(s) 273, 1783, 1894, 1952, 2058
Alw26I GTCTC 6 cut(s) 616, 769, 1032, 1305, 1664, 1845
AlwI GGATC 4 cut(s) 12, 434, 1064, 1722
Aor51HI AGCGCT 1 cut(s) 1913
AoxI GGCC 8 cut(s) 857, 889, 1838, 1861, 1895, 1953, 1976, 2076
ApoI RAATTY 3 cut(s) 128, 929, 1221
AsiGI ACCGGT 2 cut(s) 1423, 1645
AspLEI GCGC 4 cut(s) 470, 523, 1914, 1966
AspS9I GGNCC 3 cut(s) 317, 889, 1699
AsuC2I CCSGG 1 cut(s) 1830
AsuHPI GGTGA 5 cut(s) 47, 107, 430, 471, 2039
AsuII TTCGAA 1 cut(s) 1930
AvaII GGWCC 2 cut(s) 317, 1699
BaeI ACNNNNGTAYC 2 cut(s) 141, 174
BalI TGGCCA 1 cut(s) 1863
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 4 cut(s) 1274, 1894, 1952, 2058
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 5 cut(s) 273, 1783, 1894, 1952, 2058
BccI CCATC 7 cut(s) 566, 681, 1400, 1715, 1727, 1736, 1934
BceAI ACGGC 3 cut(s) 1004, 1254, 1432
BcgI CGANNNNNNTGC 2 cut(s) 1955, 1989
BciT130I CCWGG 3 cut(s) 1398, 1664, 2001
BciVI GTATCC 1 cut(s) 367
BclI TGATCA 2 cut(s) 169, 1081
BcnI CCSGG 1 cut(s) 1830
BcoDI GTCTC 6 cut(s) 616, 769, 1032, 1305, 1664, 1845
BfaI CTAG 4 cut(s) 164, 1016, 1074, 1451
BfmI CTRYAG 2 cut(s) 909, 1380
BfoI RGCGCY 2 cut(s) 471, 1915
BfrI CTTAAG 1 cut(s) 725
BfuAI ACCTGC 1 cut(s) 809
BfuI GTATCC 1 cut(s) 367
Bme1390I CCNGG 4 cut(s) 1398, 1664, 1830, 2001
Bme18I GGWCC 2 cut(s) 317, 1699
BmgT120I GGNCC 3 cut(s) 317, 889, 1699
BmiI GGNNCC 5 cut(s) 6, 568, 722, 1556, 1700
BmrFI CCNGG 4 cut(s) 1398, 1664, 1830, 2001
BmsI GCATC 3 cut(s) 316, 667, 1951
BpiI GAAGAC 1 cut(s) 139
BpmI CTGGAG 1 cut(s) 1419
Bpu10I CCTNAGC 1 cut(s) 960
Bpu14I TTCGAA 1 cut(s) 1930
BpuMI CCSGG 1 cut(s) 1830
Bsa29I ATCGAT 1 cut(s) 1490
BsaBI GATNNNNATC 1 cut(s) 59
BsaHI GRCGYC 2 cut(s) 625, 1470
BsaJI CCNNGG 5 cut(s) 594, 1251, 1396, 1558, 2000
BsaWI WCCGGW 4 cut(s) 32, 1423, 1637, 1645
BsaXI ACNNNNNCTCC 4 cut(s) 1209, 1239, 1460, 1490
Bsc4I CCNNNNNNNGG 7 cut(s) 366, 808, 1547, 1637, 1836, 2006, 2024
Bse118I RCCGGY 3 cut(s) 1423, 1645, 1978
Bse1I ACTGG 1 cut(s) 1135
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 59
BseBI CCWGG 3 cut(s) 1398, 1664, 2001
BseCI ATCGAT 1 cut(s) 1490
BseDI CCNNGG 5 cut(s) 594, 1251, 1396, 1558, 2000
BseGI GGATG 3 cut(s) 29, 692, 1392
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 7 cut(s) 366, 808, 1547, 1637, 1836, 2006, 2024
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 1135
BseX3I CGGCCG 1 cut(s) 1976
BseYI CCCAGC 1 cut(s) 1546
BsgI GTGCAG 3 cut(s) 203, 837, 2060
Bsh1236I CGCG 4 cut(s) 523, 563, 596, 809
Bsh1285I CGRYCG 2 cut(s) 1119, 1979
BshFI GGCC 8 cut(s) 859, 891, 1840, 1863, 1897, 1955, 1978, 2078
BshTI ACCGGT 2 cut(s) 1423, 1645
BshVI ATCGAT 1 cut(s) 1490
BsiEI CGRYCG 2 cut(s) 1119, 1979
BsiHKAI GWGCWC 5 cut(s) 273, 1783, 1894, 1952, 2058
BsiSI CCGG 8 cut(s) 33, 1424, 1638, 1646, 1750, 1830, 1979, 2018
BslFI GGGAC 1 cut(s) 1563
BslI CCNNNNNNNGG 7 cut(s) 366, 808, 1547, 1637, 1836, 2006, 2024
BsmAI GTCTC 6 cut(s) 616, 769, 1032, 1305, 1664, 1845
BsmBI CGTCTC 2 cut(s) 616, 1305
BsmFI GGGAC 1 cut(s) 1563
BsmI GAATGC 2 cut(s) 1442, 1765
BsnI GGCC 8 cut(s) 859, 891, 1840, 1863, 1897, 1955, 1978, 2078
Bsp119I TTCGAA 1 cut(s) 1930
Bsp1286I GDGCHC 6 cut(s) 273, 1274, 1783, 1894, 1952, 2058
BspANI GGCC 8 cut(s) 859, 891, 1840, 1863, 1897, 1955, 1978, 2078
BspCNI CTCAG 1 cut(s) 93
BspDI ATCGAT 1 cut(s) 1490
BspFNI CGCG 4 cut(s) 523, 563, 596, 809
BspHI TCATGA 1 cut(s) 2050
BspLI GGNNCC 5 cut(s) 6, 568, 722, 1556, 1700
BspMAI CTGCAG 2 cut(s) 913, 1384
BspMI ACCTGC 1 cut(s) 809
BspPI GGATC 4 cut(s) 12, 434, 1064, 1722
BspQI GCTCTTC 2 cut(s) 491, 1771
BspT104I TTCGAA 1 cut(s) 1930
BspTI CTTAAG 1 cut(s) 725
BsrBI CCGCTC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 250
BsrFI RCCGGY 3 cut(s) 1423, 1645, 1978
BsrI ACTGG 1 cut(s) 1135
BssAI RCCGGY 3 cut(s) 1423, 1645, 1978
BssECI CCNNGG 5 cut(s) 594, 1251, 1396, 1558, 2000
BssNI GRCGYC 2 cut(s) 625, 1470
BssT1I CCWWGG 2 cut(s) 1251, 1558
Bst2UI CCWGG 3 cut(s) 1398, 1664, 2001
Bst4CI ACNGT 5 cut(s) 1090, 1175, 1369, 1554, 1822
Bst6I CTCTTC 4 cut(s) 272, 491, 1181, 1771
BstACI GRCGYC 2 cut(s) 625, 1470
BstAFI CTTAAG 1 cut(s) 725
BstBI TTCGAA 1 cut(s) 1930
BstC8I GCNNGC 7 cut(s) 591, 1202, 1299, 1910, 1968, 1994, 2015
BstDEI CTNAG 3 cut(s) 80, 916, 960
BstDSI CCRYGG 1 cut(s) 594
BstF5I GGATG 3 cut(s) 29, 692, 1392
BstFNI CGCG 4 cut(s) 523, 563, 596, 809
BstH2I RGCGCY 2 cut(s) 471, 1915
BstHHI GCGC 4 cut(s) 470, 523, 1914, 1966
BstMAI GTCTC 6 cut(s) 616, 769, 1032, 1305, 1664, 1845
BstMCI CGRYCG 2 cut(s) 1119, 1979
BstMWI GCNNNNNNNGC 6 cut(s) 590, 624, 815, 1846, 1961, 1970
BstNI CCWGG 3 cut(s) 1398, 1664, 2001
BstNSI RCATGY 4 cut(s) 593, 949, 1115, 1540
BstSCI CCNGG 4 cut(s) 1396, 1662, 1828, 1999
BstSFI CTRYAG 2 cut(s) 909, 1380
BstUI CGCG 4 cut(s) 523, 563, 596, 809
BstV2I GAAGAC 1 cut(s) 139
BstX2I RGATCY 2 cut(s) 4, 1727
BstXI CCANNNNNNTGG 1 cut(s) 1345
BstYI RGATCY 2 cut(s) 4, 1727
BstZI CGGCCG 1 cut(s) 1976
Bsu15I ATCGAT 1 cut(s) 1490
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 8 cut(s) 859, 891, 1840, 1863, 1897, 1955, 1978, 2078
BsuTUI ATCGAT 1 cut(s) 1490
BtgI CCRYGG 1 cut(s) 594
BtsCI GGATG 3 cut(s) 29, 692, 1392
BveI ACCTGC 1 cut(s) 809
Cac8I GCNNGC 7 cut(s) 591, 1202, 1299, 1910, 1968, 1994, 2015
CciI TCATGA 1 cut(s) 2050
CfoI GCGC 4 cut(s) 470, 523, 1914, 1966
Cfr10I RCCGGY 3 cut(s) 1423, 1645, 1978
Cfr13I GGNCC 3 cut(s) 317, 889, 1699
Cfr42I CCGCGG 1 cut(s) 597
ClaI ATCGAT 1 cut(s) 1490
CseI GACGC 2 cut(s) 633, 1478
Csp6I GTAC 2 cut(s) 158, 943
CspAI ACCGGT 2 cut(s) 1423, 1645
CviAII CATG 9 cut(s) 386, 590, 619, 905, 946, 1112, 1229, 1537, 2051
CviQI GTAC 2 cut(s) 158, 943
DdeI CTNAG 3 cut(s) 80, 916, 960
DrdI GACNNNNNNGTC 1 cut(s) 1804
DseDI GACNNNNNNGTC 1 cut(s) 1804
EaeI YGGCCR 4 cut(s) 1861, 1895, 1953, 1976
EagI CGGCCG 1 cut(s) 1976
Eam1104I CTCTTC 4 cut(s) 272, 491, 1181, 1771
EarI CTCTTC 4 cut(s) 272, 491, 1181, 1771
EciI GGCGGA 2 cut(s) 1752, 1848
Ecl136II GAGCTC 3 cut(s) 1892, 1950, 2056
EclXI CGGCCG 1 cut(s) 1976
Eco130I CCWWGG 2 cut(s) 1251, 1558
Eco24I GRGCYC 4 cut(s) 1274, 1894, 1952, 2058
Eco47I GGWCC 2 cut(s) 317, 1699
Eco47III AGCGCT 1 cut(s) 1913
Eco52I CGGCCG 1 cut(s) 1976
Eco53kI GAGCTC 3 cut(s) 1892, 1950, 2056
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 3 cut(s) 1892, 1950, 2056
EcoRI GAATTC 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 1396, 1662, 1999
EcoT14I CCWWGG 2 cut(s) 1251, 1558
EcoT38I GRGCYC 4 cut(s) 1274, 1894, 1952, 2058
ErhI CCWWGG 2 cut(s) 1251, 1558
Esp3I CGTCTC 2 cut(s) 616, 1305
FaeI CATG 9 cut(s) 389, 593, 622, 908, 949, 1115, 1232, 1540, 2054
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FaqI GGGAC 1 cut(s) 1563
FatI CATG 9 cut(s) 385, 589, 618, 904, 945, 1111, 1228, 1536, 2050
FauI CCCGC 3 cut(s) 556, 1756, 1912
FbaI TGATCA 2 cut(s) 169, 1081
FblI GTMKAC 4 cut(s) 922, 1119, 1533, 1796
FokI GGATG 3 cut(s) 16, 699, 1379
FriOI GRGCYC 4 cut(s) 1274, 1894, 1952, 2058
FspBI CTAG 4 cut(s) 164, 1016, 1074, 1451
FspI TGCGCA 1 cut(s) 1965
GlaI GCGC 4 cut(s) 469, 522, 1913, 1965
GsaI CCCAGC 1 cut(s) 1550
GsuI CTGGAG 1 cut(s) 1419
HaeII RGCGCY 2 cut(s) 471, 1915
HaeIII GGCC 8 cut(s) 859, 891, 1840, 1863, 1897, 1955, 1978, 2078
HapII CCGG 8 cut(s) 33, 1424, 1638, 1646, 1750, 1830, 1979, 2018
HgaI GACGC 2 cut(s) 633, 1478
HhaI GCGC 4 cut(s) 470, 523, 1914, 1966
Hin1I GRCGYC 2 cut(s) 625, 1470
Hin1II CATG 9 cut(s) 389, 593, 622, 908, 949, 1115, 1232, 1540, 2054
Hin6I GCGC 4 cut(s) 468, 521, 1912, 1964
HinP1I GCGC 4 cut(s) 468, 521, 1912, 1964
HincII GTYRAC 5 cut(s) 923, 1120, 1534, 1643, 1797
HindII GTYRAC 5 cut(s) 923, 1120, 1534, 1643, 1797
HindIII AAGCTT 2 cut(s) 962, 1317
HinfI GANTC 6 cut(s) 476, 847, 955, 1103, 1404, 1627
HpaII CCGG 8 cut(s) 33, 1424, 1638, 1646, 1750, 1830, 1979, 2018
HphI GGTGA 5 cut(s) 47, 107, 430, 471, 2039
Hpy166II GTNNAC 8 cut(s) 317, 421, 923, 1120, 1534, 1643, 1797, 1925
Hpy188I TCNGA 7 cut(s) 87, 408, 1050, 1086, 1468, 1732, 1889
Hpy188III TCNNGA 6 cut(s) 49, 443, 776, 786, 969, 2051
Hpy8I GTNNAC 8 cut(s) 317, 421, 923, 1120, 1534, 1643, 1797, 1925
Hpy99I CGWCG 5 cut(s) 1270, 1369, 1810, 1846, 1872
HpyAV CCTTC 9 cut(s) 86, 364, 564, 989, 1483, 1489, 1592, 1687, 1830
HpyCH4III ACNGT 5 cut(s) 1090, 1175, 1369, 1554, 1822
HpyCH4IV ACGT 5 cut(s) 39, 144, 222, 1265, 1801
HpyF10VI GCNNNNNNNGC 6 cut(s) 590, 624, 815, 1846, 1961, 1970
HpyF3I CTNAG 3 cut(s) 80, 916, 960
HpySE526I ACGT 5 cut(s) 39, 144, 222, 1265, 1801
Hsp92I GRCGYC 2 cut(s) 625, 1470
Hsp92II CATG 9 cut(s) 389, 593, 622, 908, 949, 1115, 1232, 1540, 2054
HspAI GCGC 4 cut(s) 468, 521, 1912, 1964
Ksp22I TGATCA 2 cut(s) 169, 1081
KspI CCGCGG 1 cut(s) 597
LguI GCTCTTC 2 cut(s) 491, 1771
LmnI GCTCC 2 cut(s) 1846, 2020
LweI GCATC 3 cut(s) 316, 667, 1951
MaeI CTAG 4 cut(s) 164, 1016, 1074, 1451
MaeII ACGT 5 cut(s) 39, 144, 222, 1265, 1801
MbiI CCGCTC 1 cut(s) 484
MfeI CAATTG 2 cut(s) 681, 1343
MflI RGATCY 2 cut(s) 4, 1727
MhlI GDGCHC 6 cut(s) 273, 1274, 1783, 1894, 1952, 2058
MlsI TGGCCA 1 cut(s) 1863
MluNI TGGCCA 1 cut(s) 1863
MlyI GAGTC 1 cut(s) 485
Mox20I TGGCCA 1 cut(s) 1863
MscI TGGCCA 1 cut(s) 1863
MseI TTAA 6 cut(s) 428, 533, 726, 1143, 1691, 2083
MslI CAYNNNNRTG 1 cut(s) 830
Msp20I TGGCCA 1 cut(s) 1863
MspA1I CMGCKG 5 cut(s) 181, 302, 323, 596, 1546
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 8 cut(s) 33, 1424, 1638, 1646, 1750, 1830, 1979, 2018
MspR9I CCNGG 4 cut(s) 1398, 1664, 1830, 2001
MunI CAATTG 2 cut(s) 681, 1343
Mva1269I GAATGC 2 cut(s) 1442, 1765
MvaI CCWGG 3 cut(s) 1398, 1664, 2001
MvnI CGCG 4 cut(s) 523, 563, 596, 809
MwoI GCNNNNNNNGC 6 cut(s) 590, 624, 815, 1846, 1961, 1970
NciI CCSGG 1 cut(s) 1830
NlaIII CATG 9 cut(s) 389, 593, 622, 908, 949, 1115, 1232, 1540, 2054
NlaIV GGNNCC 5 cut(s) 6, 568, 722, 1556, 1700
NmeAIII GCCGAG 5 cut(s) 715, 1873, 1895, 1931, 1954
NmuCI GTSAC 5 cut(s) 35, 477, 577, 1412, 1522
NsbI TGCGCA 1 cut(s) 1965
NspI RCATGY 4 cut(s) 593, 949, 1115, 1540
NspV TTCGAA 1 cut(s) 1930
OliI CACNNNNGTG 1 cut(s) 830
PaeI GCATGC 1 cut(s) 593
PagI TCATGA 1 cut(s) 2050
PciI ACATGT 1 cut(s) 945
PciSI GCTCTTC 2 cut(s) 491, 1771
PcsI WCGNNNNNNNCGW 2 cut(s) 567, 1802
PctI GAATGC 2 cut(s) 1442, 1765
PfeI GAWTC 5 cut(s) 847, 955, 1103, 1404, 1627
PinAI ACCGGT 2 cut(s) 1423, 1645
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PscI ACATGT 1 cut(s) 945
PsiI TTATAA 1 cut(s) 977
Psp124BI GAGCTC 3 cut(s) 1894, 1952, 2058
Psp6I CCWGG 3 cut(s) 1396, 1662, 1999
PspFI CCCAGC 1 cut(s) 1546
PspGI CCWGG 3 cut(s) 1396, 1662, 1999
PspN4I GGNNCC 5 cut(s) 6, 568, 722, 1556, 1700
PspPI GGNCC 3 cut(s) 317, 889, 1699
PstI CTGCAG 2 cut(s) 913, 1384
PsuI RGATCY 2 cut(s) 4, 1727
PvuII CAGCTG 2 cut(s) 302, 323
RsaI GTAC 2 cut(s) 159, 944
RsaNI GTAC 2 cut(s) 158, 943
RseI CAYNNNNRTG 1 cut(s) 830
SacI GAGCTC 3 cut(s) 1894, 1952, 2058
SacII CCGCGG 1 cut(s) 597
SalI GTCGAC 4 cut(s) 921, 1118, 1532, 1795
SapI GCTCTTC 2 cut(s) 491, 1771
SaqAI TTAA 6 cut(s) 428, 533, 726, 1143, 1691, 2083
Sau96I GGNCC 3 cut(s) 317, 889, 1699
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 4 cut(s) 1398, 1664, 1830, 2001
SduI GDGCHC 6 cut(s) 273, 1274, 1783, 1894, 1952, 2058
SfaNI GCATC 3 cut(s) 316, 667, 1951
SfcI CTRYAG 2 cut(s) 909, 1380
Sfr303I CCGCGG 1 cut(s) 597
SfuI TTCGAA 1 cut(s) 1930
SgrBI CCGCGG 1 cut(s) 597
SinI GGWCC 2 cut(s) 317, 1699
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SphI GCATGC 1 cut(s) 593
SspMI CTAG 4 cut(s) 164, 1016, 1074, 1451
SstI GAGCTC 3 cut(s) 1894, 1952, 2058
StyD4I CCNGG 4 cut(s) 1396, 1662, 1828, 1999
StyI CCWWGG 2 cut(s) 1251, 1558
TaaI ACNGT 5 cut(s) 1090, 1175, 1369, 1554, 1822
TaiI ACGT 5 cut(s) 42, 147, 225, 1268, 1804
TauI GCSGC 4 cut(s) 437, 596, 812, 1973
TfiI GAWTC 5 cut(s) 847, 955, 1103, 1404, 1627
Tru1I TTAA 6 cut(s) 428, 533, 726, 1143, 1691, 2083
Tru9I TTAA 6 cut(s) 428, 533, 726, 1143, 1691, 2083
TseFI GTSAC 5 cut(s) 35, 477, 577, 1412, 1522
Tsp45I GTSAC 5 cut(s) 35, 477, 577, 1412, 1522
TspDTI ATGAA 6 cut(s) 309, 374, 478, 996, 1245, 1499
TspGWI ACGGA 2 cut(s) 167, 1859
Vha464I CTTAAG 1 cut(s) 725
VpaK11BI GGWCC 2 cut(s) 317, 1699
XapI RAATTY 3 cut(s) 128, 929, 1221
XceI RCATGY 4 cut(s) 593, 949, 1115, 1540
XcmI CCANNNNNNNNNTGG 2 cut(s) 1660, 1871
XmiI GTMKAC 4 cut(s) 922, 1119, 1533, 1796
XspI CTAG 4 cut(s) 164, 1016, 1074, 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.