Rh5CG208400

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000319
Physical Location & Seq
Forward (+)
21004440 .. 21006698
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG208400.1

Sequence Viewer

Length: 978 bp
ATGTCAGCTCTCCATATCGCGGCTTGTGCAGGTCGCACTAAAGTATTGAAAGCGTTGATTCAATATCGGCCTGATACTTGTGGTTTACTCAATGATAAGGGCCAAAACATTCTCCATGCTGTAGTCTTAGGTCGACAAAGATTTATTATCAGGTACATATTGAAGAATCCTAAGCTTTCCAGACTTATAAATGAAGCGGATAAAGAAGGGAACACTCCCTTACATCTAGCCGTCATTTATTACAGAGGAGACAGACCTCTGATTAGATGTTTGACACTCAATCCTAGAGTTGATCAGACTGTTATAAATAACGAATTCTCACATGCGGTCGACATCCTTACTAGCCAGAATTTTAAACAGGGTATTCTTACTTACCATCCTCGCGGTTTTGGCTTCGAGAGAAATGTTGGCGTGCCAGCTTTTCACAAAGAAATTCTTCATGAAATCATGGCATTTGAACCTAAGGAGAACAATAGGTCGAGCCCACCAACAAGTAGAGAAAATTGGCAAGCTAATAATGGAGATAAAGCTTTGAAAACCTATGATACCACTGAATTGGTAGCGGTGCTTATTGCGACCGTAACATTTGCTGCAGCCTTTACCATACCTGGAGGATTTAAAAGTGACGGCACACCAGTTTTATATGGCAAAGCATTCTTCCCAGTGTTTCTGGTATCAAACGCCTTGTCTTTCTTTACATCTATTTTGGTTCTGTGTCTTCATTTTTTGAGGGTCACTGCTGTCGACGTACCATCGATGGGACGGCTCCTTGGAAACTTAGTATTGTATTCCCTTCAAGGAATGGTGATAGCATTTTCTTCAGGTATATTGATAATGATTCCAAAATCCGGTCAACCGGTTGCCATTTGTCTCGTGCTCATATGTGGCTTACTATGCTCGTTTATCCAAGCTTTGTTCCGCTCTGCACCTAGGAATCGCACATTTAGGATGATAGGGCTCCGCATCAGCAAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

325

Amino Acids

36.08

Weight (kDa)

9.8

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 4 - 91 1.2e-09 Ankyrin repeats (3 copies)
PGG PF13962 179 - 278 5.1e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 188, 305
Acc36I ACCTGC 1 cut(s) 20
AccBSI CCGCTC 1 cut(s) 921
AccI GTMKAC 3 cut(s) 133, 330, 744
AccII CGCG 2 cut(s) 20, 384
AciI CCGC 7 cut(s) 20, 197, 326, 384, 563, 919, 961
AcsI RAATTY 3 cut(s) 314, 349, 432
AcuI CTGAAG 1 cut(s) 804
AfaI GTAC 2 cut(s) 155, 750
AfiI CCNNNNNNNGG 3 cut(s) 19, 758, 848
AgeI ACCGGT 1 cut(s) 856
AgsI TTSAA 6 cut(s) 49, 62, 163, 458, 535, 797
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 6 cut(s) 8, 175, 419, 512, 530, 911
AluI AGCT 6 cut(s) 8, 175, 419, 512, 530, 911
Alw21I GWGCWC 1 cut(s) 879
Alw26I GTCTC 2 cut(s) 243, 875
AoxI GGCC 2 cut(s) 68, 100
ApeKI GCWGC 2 cut(s) 590, 593
ApoI RAATTY 3 cut(s) 314, 349, 432
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AsiGI ACCGGT 1 cut(s) 856
Asp700I GAANNNNTTC 1 cut(s) 435
AspA2I CCTAGG 1 cut(s) 929
AspS9I GGNCC 1 cut(s) 100
AsuHPI GGTGA 1 cut(s) 817
AvrII CCTAGG 1 cut(s) 929
AxyI CCTNAGG 1 cut(s) 462
BanII GRGCYC 2 cut(s) 485, 960
BauI CACGAG 1 cut(s) 872
BbsI GAAGAC 1 cut(s) 710
Bbv12I GWGCWC 1 cut(s) 879
BbvI GCAGC 2 cut(s) 577, 605
BccI CCATC 3 cut(s) 384, 751, 760
BceAI ACGGC 3 cut(s) 215, 643, 779
BciT130I CCWGG 1 cut(s) 609
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 243, 875
BfaI CTAG 4 cut(s) 227, 285, 342, 930
BfmI CTRYAG 2 cut(s) 120, 591
BfuAI ACCTGC 1 cut(s) 20
BisI GCNGC 3 cut(s) 21, 591, 594
BlnI CCTAGG 1 cut(s) 929
BlsI GCNGC 3 cut(s) 22, 592, 595
Bme1390I CCNGG 1 cut(s) 609
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 2 cut(s) 767, 959
BmrFI CCNGG 1 cut(s) 609
BmrI ACTGGG 1 cut(s) 656
BmsI GCATC 1 cut(s) 972
BmuI ACTGGG 1 cut(s) 656
BpiI GAAGAC 1 cut(s) 710
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 171
Bsa29I ATCGAT 1 cut(s) 755
BsaJI CCNNGG 2 cut(s) 769, 929
BsaWI WCCGGW 2 cut(s) 848, 856
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 758, 848
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 2 cut(s) 635, 662
Bse21I CCTNAGG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 609
BseCI ATCGAT 1 cut(s) 755
BseDI CCNNGG 2 cut(s) 769, 929
BseGI GGATG 3 cut(s) 333, 376, 954
BseLI CCNNNNNNNGG 3 cut(s) 19, 758, 848
BseNI ACTGG 2 cut(s) 635, 662
BseRI GAGGAG 1 cut(s) 261
BseXI GCAGC 2 cut(s) 577, 605
BsgI GTGCAG 2 cut(s) 48, 909
Bsh1236I CGCG 2 cut(s) 20, 384
Bsh1285I CGRYCG 2 cut(s) 330, 579
BshFI GGCC 2 cut(s) 70, 102
BshTI ACCGGT 1 cut(s) 856
BshVI ATCGAT 1 cut(s) 755
BsiEI CGRYCG 2 cut(s) 330, 579
BsiHKAI GWGCWC 1 cut(s) 879
BsiSI CCGG 2 cut(s) 849, 857
BslFI GGGAC 1 cut(s) 774
BslI CCNNNNNNNGG 3 cut(s) 19, 758, 848
BsmAI GTCTC 2 cut(s) 243, 875
BsmFI GGGAC 1 cut(s) 774
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 2 cut(s) 70, 102
Bsp1286I GDGCHC 3 cut(s) 485, 879, 960
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 7 cut(s) 20, 197, 326, 384, 563, 919, 961
BspANI GGCC 2 cut(s) 70, 102
BspDI ATCGAT 1 cut(s) 755
BspFNI CGCG 2 cut(s) 20, 384
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 2 cut(s) 767, 959
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 921
BsrFI RCCGGY 1 cut(s) 856
BsrI ACTGG 2 cut(s) 635, 662
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 2 cut(s) 769, 929
BssMI GATC 1 cut(s) 292
BssSI CACGAG 1 cut(s) 872
BssT1I CCWWGG 2 cut(s) 769, 929
Bst2BI CACGAG 1 cut(s) 872
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 301, 580
BstC8I GCNNGC 3 cut(s) 413, 417, 510
BstDEI CTNAG 4 cut(s) 127, 171, 462, 778
BstF5I GGATG 3 cut(s) 333, 376, 954
BstFNI CGCG 2 cut(s) 20, 384
BstKTI GATC 1 cut(s) 295
BstMAI GTCTC 2 cut(s) 243, 875
BstMBI GATC 1 cut(s) 292
BstMCI CGRYCG 2 cut(s) 330, 579
BstMWI GCNNNNNNNGC 3 cut(s) 26, 390, 894
BstNI CCWGG 1 cut(s) 609
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 607
BstSFI CTRYAG 2 cut(s) 120, 591
BstUI CGCG 2 cut(s) 20, 384
BstV1I GCAGC 2 cut(s) 577, 605
BstV2I GAAGAC 1 cut(s) 710
BstXI CCANNNNNNTGG 1 cut(s) 556
Bsu15I ATCGAT 1 cut(s) 755
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 2 cut(s) 70, 102
BsuTUI ATCGAT 1 cut(s) 755
BtsCI GGATG 3 cut(s) 333, 376, 954
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 3 cut(s) 549, 669, 735
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 3 cut(s) 413, 417, 510
CciI TCATGA 1 cut(s) 439
Cfr10I RCCGGY 1 cut(s) 856
Cfr13I GGNCC 1 cut(s) 100
ClaI ATCGAT 1 cut(s) 755
Csp6I GTAC 2 cut(s) 154, 749
CspAI ACCGGT 1 cut(s) 856
CviAII CATG 4 cut(s) 116, 323, 440, 448
CviQI GTAC 2 cut(s) 154, 749
DdeI CTNAG 4 cut(s) 127, 171, 462, 778
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
DraI TTTAAA 2 cut(s) 355, 619
Eco130I CCWWGG 2 cut(s) 769, 929
Eco24I GRGCYC 2 cut(s) 485, 960
Eco57I CTGAAG 1 cut(s) 804
Eco81I CCTNAGG 1 cut(s) 462
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 2 cut(s) 769, 929
EcoT38I GRGCYC 2 cut(s) 485, 960
ErhI CCWWGG 2 cut(s) 769, 929
FaeI CATG 4 cut(s) 119, 326, 443, 451
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 1 cut(s) 774
FatI CATG 4 cut(s) 115, 322, 439, 447
FauNDI CATATG 1 cut(s) 881
FbaI TGATCA 1 cut(s) 292
FblI GTMKAC 3 cut(s) 133, 330, 744
Fnu4HI GCNGC 3 cut(s) 21, 591, 594
FokI GGATG 3 cut(s) 320, 363, 961
FriOI GRGCYC 2 cut(s) 485, 960
Fsp4HI GCNGC 3 cut(s) 21, 591, 594
FspBI CTAG 4 cut(s) 227, 285, 342, 930
GluI GCNGC 3 cut(s) 21, 591, 594
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 2 cut(s) 70, 102
HapII CCGG 2 cut(s) 849, 857
Hin1II CATG 4 cut(s) 119, 326, 443, 451
HincII GTYRAC 4 cut(s) 134, 331, 745, 854
HindII GTYRAC 4 cut(s) 134, 331, 745, 854
HindIII AAGCTT 3 cut(s) 173, 528, 909
HinfI GANTC 4 cut(s) 58, 166, 838, 934
HpaII CCGG 2 cut(s) 849, 857
HphI GGTGA 1 cut(s) 817
Hpy166II GTNNAC 5 cut(s) 86, 134, 331, 745, 854
Hpy188I TCNGA 2 cut(s) 261, 297
Hpy188III TCNNGA 3 cut(s) 180, 397, 440
Hpy8I GTNNAC 5 cut(s) 86, 134, 331, 745, 854
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 200, 803
HpyCH4III ACNGT 2 cut(s) 301, 580
HpyCH4IV ACGT 1 cut(s) 747
HpyCH4V TGCA 3 cut(s) 29, 593, 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 390, 894
HpyF3I CTNAG 4 cut(s) 127, 171, 462, 778
HpySE526I ACGT 1 cut(s) 747
Hsp92II CATG 4 cut(s) 119, 326, 443, 451
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 2 cut(s) 771, 963
Lsp1109I GCAGC 2 cut(s) 577, 605
LweI GCATC 1 cut(s) 972
MaeI CTAG 4 cut(s) 227, 285, 342, 930
MaeII ACGT 1 cut(s) 747
MaeIII GTNAC 3 cut(s) 580, 623, 733
MalI GATC 1 cut(s) 294
MbiI CCGCTC 1 cut(s) 921
MboI GATC 1 cut(s) 292
MboII GAAGA 5 cut(s) 175, 428, 649, 710, 810
MhlI GDGCHC 3 cut(s) 485, 879, 960
MluCI AATT 5 cut(s) 314, 349, 432, 502, 554
MnlI CCTC 5 cut(s) 239, 267, 390, 605, 723
MroXI GAANNNNTTC 1 cut(s) 435
MseI TTAA 2 cut(s) 354, 618
MspI CCGG 2 cut(s) 849, 857
MspR9I CCNGG 1 cut(s) 609
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 2 cut(s) 20, 384
MwoI GCNNNNNNNGC 3 cut(s) 26, 390, 894
NdeI CATATG 1 cut(s) 881
NdeII GATC 1 cut(s) 292
NlaIII CATG 4 cut(s) 119, 326, 443, 451
NlaIV GGNNCC 2 cut(s) 767, 959
NmuCI GTSAC 2 cut(s) 623, 733
NspI RCATGY 1 cut(s) 326
PagI TCATGA 1 cut(s) 439
PctI GAATGC 1 cut(s) 653
PdmI GAANNNNTTC 1 cut(s) 435
PfeI GAWTC 4 cut(s) 58, 166, 838, 934
PinAI ACCGGT 1 cut(s) 856
PkrI GCNGC 3 cut(s) 22, 592, 595
PsiI TTATAA 2 cut(s) 188, 305
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 2 cut(s) 767, 959
PspPI GGNCC 1 cut(s) 100
PstI CTGCAG 1 cut(s) 595
RsaI GTAC 2 cut(s) 155, 750
RsaNI GTAC 2 cut(s) 154, 749
SalI GTCGAC 3 cut(s) 132, 329, 743
SaqAI TTAA 2 cut(s) 354, 618
SatI GCNGC 3 cut(s) 21, 591, 594
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 3 cut(s) 485, 879, 960
SfaNI GCATC 1 cut(s) 972
SfcI CTRYAG 2 cut(s) 120, 591
Sse9I AATT 5 cut(s) 314, 349, 432, 502, 554
SsiI CCGC 7 cut(s) 20, 197, 326, 384, 563, 919, 961
SspMI CTAG 4 cut(s) 227, 285, 342, 930
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 2 cut(s) 769, 929
TaaI ACNGT 2 cut(s) 301, 580
TaiI ACGT 1 cut(s) 750
TaqI TCGA 6 cut(s) 133, 330, 396, 479, 744, 755
TasI AATT 5 cut(s) 314, 349, 432, 502, 554
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 4 cut(s) 58, 166, 838, 934
Tru1I TTAA 2 cut(s) 354, 618
Tru9I TTAA 2 cut(s) 354, 618
TscAI CASTG 3 cut(s) 556, 669, 742
TseFI GTSAC 2 cut(s) 623, 733
TseI GCWGC 2 cut(s) 590, 593
Tsp45I GTSAC 2 cut(s) 623, 733
TspDTI ATGAA 4 cut(s) 207, 428, 456, 710
TspRI CASTG 3 cut(s) 556, 669, 742
XapI RAATTY 3 cut(s) 314, 349, 432
XceI RCATGY 1 cut(s) 326
XmaJI CCTAGG 1 cut(s) 929
XmiI GTMKAC 3 cut(s) 133, 330, 744
XmnI GAANNNNTTC 1 cut(s) 435
XspI CTAG 4 cut(s) 227, 285, 342, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.