RchiOBHm_Chr3g0482301

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000319
Physical Location & Seq
Forward (+)
28448521 .. 28451681
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44717

Sequence Viewer

Length: 1863 bp
ATGATACGTAACTCAACCTCAAGTGTAGTTGTTCAATTGTGCGTTTTGGAAATTGAGAGAGAGAGAGAGAGAATGGATCCTTCTCTCTATAGAGCAGCAAGATCTGGTGATATAAATTTCCTGAATATTGAAGATGCATGTATTGATCCTCTCCATCAAAGAACGCCTAAAGAGAACAATCTTCTTCACATCGCTGCGGAATTCAAGCAAATAGATTTCTTCAAACATGTCAAACTCAACTATGAATCTCCGCGGTTTTGGGTCACCAACAAGTCCGGCGACACTCCATTGCACGTCGCAGCCAGAGTAGGCTGTCATGCAGTTGTTGAGTTTGTCATTGACCACACAAAATCATTCCCCCTCGAAGGAGCTGATGATCAGGAAAGCGTACCAGCTGATGCTGAAACTTATAAACAACTGCTCAGAATGCCTAATTTTCAAAATGACACAGCTTTGCATATTGCTGTGCGATATGGTCACGATAAGGTAGCGACTCTACTAATCAAAGCTGACCCTGAACTCTGTTATTACACAAATAGTGCGAACGAGTCGCCGTTGTTCCTTGCTATTAGAAAGAGCTCTCCGAGCATCGCTAGTTACATTTTAGAGGAGTCTCCTTCTCATGCGTCTCCTTATCTTCAAGGGACTAATGGTTTAACGGCTTTGCACGCGGCAGTAACTCACCAAGACCTTGCAAGCAAAGGCATTGTGAAGACTATGGTCTCCACGAATCCTGAGATAATAAGAGAGCCTGAAGCACTTGGGTGGACTCCTTTACATTATGCAGCACTGATAGGGAACCTAGAAGCAACTCGAGTGCTACTTCAGTCCAATAGTTCTGTTTCTTACATAATGGATAATTCCGGGTCGTCACCTCTCCATATTGCGGCTTATGCAGGCCACACTAATGTAATGGAAGAGCTTATTCGATGTCGGCCTGATACCTGTGATCTGGTCAATGAAAATGGTCAATCAGCTCTACATGCTGCAGTTATAGGTTCACGAATTAATGTTGTCAAATACATATTGAAGACTCCTGGGCTTTCAGGACTTACAAATGAAGCAGATAAAGATGGAGACACTCCATTACATCTAGCTGCTATTTGCCGCAACCAAGACATTATAAGAATTTTGGCACAAGACAGCAGAGTAGACATTCTCTCTTTCAATAAGGAATCCACAACCGCCCTCGACATTTTTCTGGGCAACAATATTGGAGAACAGGAAATTCTTGGCAATAGTAGAACGATTTTGCACCACCTGAAGTGTTCTGTTGGTGTGCCATTTCTCCAACAACAAATCAGCCATAATTTGAAGAAACTGACAACTCGAGAGAGGGATACGTCTGACACACTGGCCATTATTGGCATCAAGAGAGAACAGCCGCAGGCTAATTTGGATCAAGCCTTGAAAAGGTATGATAATAAACTATTGGTAGCAACGCTAATCGCAACGGTCACATTTGCAGCAGGTTTCACCAATGTTCCTGGAGGATTTAAAAACGACGGATCACCAGTTTTATACGAACATATGTCTTTCAGTTTCTTCCAGTTTTGGAATATGGTATCATTCATTCTCTCTGTTCTTGCAATTCTCAATGAATCAACTCCGCTAACTGTATTATCAACCCGTTTACCCACGCCTGCAAGTCTCATTCAGTTTTCCATTGGTGGAATGGTTATAGCATTTCTCGCGGGCACGGCAACACTGCAACCCAAACATCCCCGAGGCAGTCCTGCTGATAAATTGTTCAGTGTAATGGTCCTCACTTTTCTCGTATTAGCTGTCATCCTTGTGTCTGTGTTCGTAGTTCAGTTATATAGGGTAAGAAGGATGCGGGTGCTCCTGAACGGCGTAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

620

Amino Acids

68.45

Weight (kDa)

6.5

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 29 - 116 7.7e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 62 - 171 2e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 136 - 204 3e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 211 - 280 9.1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 232 - 311 3.1e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 315 - 386 1.3e-11 Ankyrin repeats (3 copies)
PGG PF13962 476 - 568 2.2e-16 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 411, 1124
Acc36I ACCTGC 1 cut(s) 1460
AccI GTMKAC 1 cut(s) 1152
AccII CGCG 3 cut(s) 253, 671, 1694
AclWI GGATC 5 cut(s) 71, 84, 140, 1407, 1516
AcoI YGGCCR 1 cut(s) 1356
AcsI RAATTY 4 cut(s) 115, 200, 1128, 1227
AcuI CTGAAG 3 cut(s) 774, 809, 1283
AfaI GTAC 1 cut(s) 390
AfiI CCNNNNNNNGG 3 cut(s) 365, 886, 1413
AflIII ACRYGT 1 cut(s) 226
AjiI CACGTC 1 cut(s) 295
AjnI CCWGG 2 cut(s) 1036, 1486
AleI CACNNNNGTG 1 cut(s) 763
AluBI AGCT 9 cut(s) 371, 395, 452, 509, 579, 922, 977, 1097, 1784
AluI AGCT 9 cut(s) 371, 395, 452, 509, 579, 922, 977, 1097, 1784
Alw21I GWGCWC 2 cut(s) 581, 1845
Alw26I GTCTC 5 cut(s) 618, 633, 727, 1071, 1655
AlwI GGATC 5 cut(s) 71, 84, 140, 1407, 1516
Ama87I CYCGRG 3 cut(s) 813, 1329, 1725
AoxI GGCC 3 cut(s) 898, 935, 1356
ApeKI GCWGC 7 cut(s) 95, 194, 299, 785, 986, 1097, 1466
ApoI RAATTY 4 cut(s) 115, 200, 1128, 1227
AseI ATTAAT 1 cut(s) 1008
AspS9I GGNCC 1 cut(s) 1762
AsuC2I CCSGG 1 cut(s) 865
AsuHPI GGTGA 6 cut(s) 119, 256, 674, 864, 1468, 1503
AvaI CYCGRG 3 cut(s) 813, 1329, 1725
AvaII GGWCC 1 cut(s) 1762
BaeGI GKGCMC 1 cut(s) 1700
BalI TGGCCA 1 cut(s) 1358
BamHI GGATCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 581
BbsI GAAGAC 2 cut(s) 719, 1037
Bbv12I GWGCWC 2 cut(s) 581, 1845
BbvI GCAGC 7 cut(s) 107, 181, 311, 797, 973, 1084, 1478
BccI CCATC 2 cut(s) 162, 1067
BceAI ACGGC 3 cut(s) 538, 675, 1716
BciT130I CCWGG 2 cut(s) 1038, 1488
BciVI GTATCC 1 cut(s) 1333
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 1 cut(s) 865
BcoDI GTCTC 5 cut(s) 618, 633, 727, 1071, 1655
BfaI CTAG 3 cut(s) 594, 803, 1094
BfmI CTRYAG 2 cut(s) 88, 987
BfuAI ACCTGC 1 cut(s) 1460
BfuI GTATCC 1 cut(s) 1333
BglII AGATCT 1 cut(s) 101
Bme1390I CCNGG 3 cut(s) 865, 1038, 1488
Bme18I GGWCC 1 cut(s) 1762
BmeT110I CYCGRG 3 cut(s) 813, 1329, 1725
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 1762
BmiI GGNNCC 2 cut(s) 78, 800
BmrFI CCNGG 3 cut(s) 865, 1038, 1488
BmsI GCATC 5 cut(s) 124, 388, 597, 1377, 1824
BoxI GACNNNNGTC 1 cut(s) 719
BpiI GAAGAC 2 cut(s) 719, 1037
BpmI CTGGAG 1 cut(s) 1509
BpuEI CTTGAG 1 cut(s) 4
BpuMI CCSGG 1 cut(s) 865
BsaAI YACGTR 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 727
BsaJI CCNNGG 3 cut(s) 251, 1037, 1726
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 886, 1413
Bse1I ACTGG 3 cut(s) 1359, 1514, 1549
Bse3DI GCAATG 1 cut(s) 287
BseBI CCWGG 2 cut(s) 1038, 1488
BseDI CCNNGG 3 cut(s) 251, 1037, 1726
BseGI GGATG 3 cut(s) 1720, 1788, 1839
BseLI CCNNNNNNNGG 3 cut(s) 365, 886, 1413
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 2 cut(s) 436, 726
BseNI ACTGG 3 cut(s) 1359, 1514, 1549
BseRI GAGGAG 1 cut(s) 623
BseSI GKGCMC 1 cut(s) 1700
BseXI GCAGC 7 cut(s) 107, 181, 311, 797, 973, 1084, 1478
Bsh1236I CGCG 3 cut(s) 253, 671, 1694
BshFI GGCC 3 cut(s) 900, 937, 1358
BsiHKAI GWGCWC 2 cut(s) 581, 1845
BsiHKCI CYCGRG 3 cut(s) 813, 1329, 1725
BsiSI CCGG 2 cut(s) 276, 864
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 3 cut(s) 365, 886, 1413
BsmAI GTCTC 5 cut(s) 618, 633, 727, 1071, 1655
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 1 cut(s) 432
BsnI GGCC 3 cut(s) 900, 937, 1358
Bso31I GGTCTC 1 cut(s) 727
BsoBI CYCGRG 3 cut(s) 813, 1329, 1725
Bsp1286I GDGCHC 3 cut(s) 581, 1700, 1845
Bsp143I GATC 7 cut(s) 76, 101, 145, 376, 949, 1399, 1508
BspANI GGCC 3 cut(s) 900, 937, 1358
BspCNI CTCAG 2 cut(s) 435, 727
BspFNI CGCG 3 cut(s) 253, 671, 1694
BspLI GGNNCC 2 cut(s) 78, 800
BspMAI CTGCAG 1 cut(s) 991
BspMI ACCTGC 1 cut(s) 1460
BspPI GGATC 5 cut(s) 71, 84, 140, 1407, 1516
BspQI GCTCTTC 1 cut(s) 912
BspTNI GGTCTC 1 cut(s) 727
BsrDI GCAATG 1 cut(s) 287
BsrI ACTGG 3 cut(s) 1359, 1514, 1549
BssECI CCNNGG 3 cut(s) 251, 1037, 1726
BssMI GATC 7 cut(s) 76, 101, 145, 376, 949, 1399, 1508
Bst2UI CCWGG 2 cut(s) 1038, 1488
Bst4CI ACNGT 2 cut(s) 1456, 1618
Bst6I CTCTTC 1 cut(s) 912
BstBAI YACGTR 1 cut(s) 8
BstC8I GCNNGC 6 cut(s) 669, 697, 898, 1389, 1644, 1696
BstDEI CTNAG 2 cut(s) 422, 735
BstDSI CCRYGG 1 cut(s) 251
BstEII GGTNACC 1 cut(s) 262
BstENI CCTNNNNNAGG 1 cut(s) 1411
BstF5I GGATG 3 cut(s) 1720, 1788, 1839
BstFNI CGCG 3 cut(s) 253, 671, 1694
BstKTI GATC 7 cut(s) 79, 104, 148, 379, 952, 1402, 1511
BstMAI GTCTC 5 cut(s) 618, 633, 727, 1071, 1655
BstMBI GATC 7 cut(s) 76, 101, 145, 376, 949, 1399, 1508
BstMWI GCNNNNNNNGC 7 cut(s) 427, 585, 668, 893, 983, 1691, 1700
BstNI CCWGG 2 cut(s) 1038, 1488
BstNSI RCATGY 3 cut(s) 141, 230, 986
BstPAI GACNNNNGTC 1 cut(s) 719
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 3 cut(s) 863, 1036, 1486
BstSFI CTRYAG 2 cut(s) 88, 987
BstSLI GKGCMC 1 cut(s) 1700
BstSNI TACGTA 1 cut(s) 8
BstUI CGCG 3 cut(s) 253, 671, 1694
BstV1I GCAGC 7 cut(s) 107, 181, 311, 797, 973, 1084, 1478
BstV2I GAAGAC 2 cut(s) 719, 1037
BstX2I RGATCY 2 cut(s) 76, 101
BstYI RGATCY 2 cut(s) 76, 101
BsuI GTATCC 1 cut(s) 1333
BsuRI GGCC 3 cut(s) 900, 937, 1358
BtgI CCRYGG 1 cut(s) 251
BtgZI GCGATG 2 cut(s) 175, 574
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 3 cut(s) 1720, 1788, 1839
BtsI GCAGTG 1 cut(s) 1706
BtsIMutI CAGTG 4 cut(s) 788, 1352, 1706, 1759
BveI ACCTGC 1 cut(s) 1460
Cac8I GCNNGC 6 cut(s) 669, 697, 898, 1389, 1644, 1696
Cfr13I GGNCC 1 cut(s) 1762
Cfr42I CCGCGG 1 cut(s) 254
CseI GACGC 1 cut(s) 615
Csp6I GTAC 1 cut(s) 389
CviAII CATG 5 cut(s) 138, 227, 317, 623, 983
CviQI GTAC 1 cut(s) 389
DdeI CTNAG 2 cut(s) 422, 735
DpnI GATC 7 cut(s) 78, 103, 147, 378, 951, 1401, 1510
DpnII GATC 7 cut(s) 76, 101, 145, 376, 949, 1399, 1508
DraI TTTAAA 1 cut(s) 1498
EaeI YGGCCR 1 cut(s) 1356
Eam1104I CTCTTC 1 cut(s) 912
EarI CTCTTC 1 cut(s) 912
Ecl136II GAGCTC 1 cut(s) 579
Eco105I TACGTA 1 cut(s) 8
Eco24I GRGCYC 1 cut(s) 581
Eco31I GGTCTC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 1762
Eco53kI GAGCTC 1 cut(s) 579
Eco57I CTGAAG 3 cut(s) 774, 809, 1283
Eco88I CYCGRG 3 cut(s) 813, 1329, 1725
Eco91I GGTNACC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 579
EcoNI CCTNNNNNAGG 1 cut(s) 1411
EcoO65I GGTNACC 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 2 cut(s) 1036, 1486
EcoT22I ATGCAT 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 581
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 5 cut(s) 141, 230, 320, 626, 986
FaqI GGGAC 1 cut(s) 658
FatI CATG 5 cut(s) 137, 226, 316, 622, 982
FauI CCCGC 2 cut(s) 1687, 1830
FauNDI CATATG 1 cut(s) 1530
FbaI TGATCA 1 cut(s) 376
FblI GTMKAC 1 cut(s) 1152
FokI GGATG 3 cut(s) 1707, 1775, 1846
FriOI GRGCYC 1 cut(s) 581
FspBI CTAG 3 cut(s) 594, 803, 1094
GsuI CTGGAG 1 cut(s) 1509
HaeIII GGCC 3 cut(s) 900, 937, 1358
HapII CCGG 2 cut(s) 276, 864
HgaI GACGC 1 cut(s) 615
Hin1II CATG 5 cut(s) 141, 230, 320, 626, 986
HinfI GANTC 9 cut(s) 245, 493, 548, 611, 730, 769, 1033, 1175, 1601
HpaII CCGG 2 cut(s) 276, 864
HphI GGTGA 6 cut(s) 119, 256, 674, 864, 1468, 1503
Hpy166II GTNNAC 4 cut(s) 768, 1001, 1153, 1634
Hpy188I TCNGA 3 cut(s) 425, 585, 1348
Hpy188III TCNNGA 9 cut(s) 121, 380, 479, 734, 1002, 1047, 1331, 1372, 1846
Hpy8I GTNNAC 4 cut(s) 768, 1001, 1153, 1634
Hpy99I CGWCG 2 cut(s) 299, 1508
HpyAV CCTTC 4 cut(s) 90, 359, 627, 1824
HpyCH4III ACNGT 2 cut(s) 1456, 1618
HpyCH4IV ACGT 3 cut(s) 7, 294, 1343
HpyF10VI GCNNNNNNNGC 7 cut(s) 427, 585, 668, 893, 983, 1691, 1700
HpyF3I CTNAG 2 cut(s) 422, 735
HpySE526I ACGT 3 cut(s) 7, 294, 1343
Hsp92II CATG 5 cut(s) 141, 230, 320, 626, 986
Ksp22I TGATCA 1 cut(s) 376
KspI CCGCGG 1 cut(s) 254
Kzo9I GATC 7 cut(s) 76, 101, 145, 376, 949, 1399, 1508
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 2 cut(s) 368, 1848
Lsp1109I GCAGC 7 cut(s) 107, 181, 311, 797, 973, 1084, 1478
LweI GCATC 5 cut(s) 124, 388, 597, 1377, 1824
MaeI CTAG 3 cut(s) 594, 803, 1094
MaeII ACGT 3 cut(s) 7, 294, 1343
MaeIII GTNAC 7 cut(s) 8, 262, 476, 596, 676, 870, 1456
MalI GATC 7 cut(s) 78, 103, 147, 378, 951, 1401, 1510
MboI GATC 7 cut(s) 76, 101, 145, 376, 949, 1399, 1508
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 2 cut(s) 76, 101
MhlI GDGCHC 3 cut(s) 581, 1700, 1845
MlsI TGGCCA 1 cut(s) 1358
MluNI TGGCCA 1 cut(s) 1358
MlyI GAGTC 5 cut(s) 487, 557, 620, 763, 1027
MmeI TCCRAC 1 cut(s) 1315
Mox20I TGGCCA 1 cut(s) 1358
Mph1103I ATGCAT 1 cut(s) 139
MscI TGGCCA 1 cut(s) 1358
MseI TTAA 3 cut(s) 656, 1008, 1497
MslI CAYNNNNRTG 3 cut(s) 763, 906, 1793
Msp20I TGGCCA 1 cut(s) 1358
MspA1I CMGCKG 2 cut(s) 253, 395
MspI CCGG 2 cut(s) 276, 864
MspR9I CCNGG 3 cut(s) 865, 1038, 1488
MunI CAATTG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 432
MvaI CCWGG 2 cut(s) 1038, 1488
MvnI CGCG 3 cut(s) 253, 671, 1694
MwoI GCNNNNNNNGC 7 cut(s) 427, 585, 668, 893, 983, 1691, 1700
NciI CCSGG 1 cut(s) 865
NdeI CATATG 1 cut(s) 1530
NdeII GATC 7 cut(s) 76, 101, 145, 376, 949, 1399, 1508
NlaIII CATG 5 cut(s) 141, 230, 320, 626, 986
NlaIV GGNNCC 2 cut(s) 78, 800
NmuCI GTSAC 4 cut(s) 262, 476, 870, 1456
NsiI ATGCAT 1 cut(s) 139
NspI RCATGY 3 cut(s) 141, 230, 986
OliI CACNNNNGTG 1 cut(s) 763
PaeR7I CTCGAG 2 cut(s) 813, 1329
PciI ACATGT 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 912
PctI GAATGC 1 cut(s) 432
PfeI GAWTC 4 cut(s) 245, 730, 1175, 1601
PfoI TCCNGGA 1 cut(s) 1486
PleI GAGTC 5 cut(s) 487, 556, 619, 763, 1027
PpsI GAGTC 5 cut(s) 487, 556, 619, 763, 1027
Ppu21I YACGTR 1 cut(s) 8
PscI ACATGT 1 cut(s) 226
PshAI GACNNNNGTC 1 cut(s) 719
PshBI ATTAAT 1 cut(s) 1008
PsiI TTATAA 2 cut(s) 411, 1124
Psp124BI GAGCTC 1 cut(s) 581
Psp6I CCWGG 2 cut(s) 1036, 1486
PspEI GGTNACC 1 cut(s) 262
PspGI CCWGG 2 cut(s) 1036, 1486
PspN4I GGNNCC 2 cut(s) 78, 800
PspPI GGNCC 1 cut(s) 1762
PspXI VCTCGAGB 1 cut(s) 813
PstI CTGCAG 1 cut(s) 991
PsuI RGATCY 2 cut(s) 76, 101
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 1 cut(s) 390
RsaNI GTAC 1 cut(s) 389
RseI CAYNNNNRTG 3 cut(s) 763, 906, 1793
SacI GAGCTC 1 cut(s) 581
SacII CCGCGG 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 3 cut(s) 656, 1008, 1497
Sau3AI GATC 7 cut(s) 76, 101, 145, 376, 949, 1399, 1508
Sau96I GGNCC 1 cut(s) 1762
SchI GAGTC 5 cut(s) 487, 557, 620, 763, 1027
ScrFI CCNGG 3 cut(s) 865, 1038, 1488
SduI GDGCHC 3 cut(s) 581, 1700, 1845
SfaNI GCATC 5 cut(s) 124, 388, 597, 1377, 1824
SfcI CTRYAG 2 cut(s) 88, 987
Sfr274I CTCGAG 2 cut(s) 813, 1329
Sfr303I CCGCGG 1 cut(s) 254
SgrBI CCGCGG 1 cut(s) 254
SinI GGWCC 1 cut(s) 1762
SlaI CTCGAG 2 cut(s) 813, 1329
SmiMI CAYNNNNRTG 3 cut(s) 763, 906, 1793
SmlI CTYRAG 3 cut(s) 19, 813, 1329
SmoI CTYRAG 3 cut(s) 19, 813, 1329
SnaBI TACGTA 1 cut(s) 8
SspI AATATT 2 cut(s) 127, 1213
SspMI CTAG 3 cut(s) 594, 803, 1094
SstI GAGCTC 1 cut(s) 581
StyD4I CCNGG 3 cut(s) 863, 1036, 1486
TaaI ACNGT 2 cut(s) 1456, 1618
TaiI ACGT 3 cut(s) 10, 297, 1346
TaqI TCGA 5 cut(s) 363, 814, 928, 1191, 1330
TauI GCSGC 4 cut(s) 674, 890, 1110, 1387
TfiI GAWTC 4 cut(s) 245, 730, 1175, 1601
Tru1I TTAA 3 cut(s) 656, 1008, 1497
Tru9I TTAA 3 cut(s) 656, 1008, 1497
TscAI CASTG 4 cut(s) 795, 1359, 1713, 1759
TseFI GTSAC 4 cut(s) 262, 476, 870, 1456
TseI GCWGC 7 cut(s) 95, 194, 299, 785, 986, 1097, 1466
Tsp45I GTSAC 4 cut(s) 262, 476, 870, 1456
TspDTI ATGAA 5 cut(s) 258, 975, 1074, 1561, 1614
TspGWI ACGGA 1 cut(s) 1521
TspRI CASTG 4 cut(s) 795, 1359, 1713, 1759
VpaK11BI GGWCC 1 cut(s) 1762
VspI ATTAAT 1 cut(s) 1008
XagI CCTNNNNNAGG 1 cut(s) 1411
XapI RAATTY 4 cut(s) 115, 200, 1128, 1227
XceI RCATGY 3 cut(s) 141, 230, 986
XcmI CCANNNNNNNNNTGG 1 cut(s) 1672
XhoI CTCGAG 2 cut(s) 813, 1329
XmiI GTMKAC 1 cut(s) 1152
XspI CTAG 3 cut(s) 594, 803, 1094
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.