Rh5BG189200

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000319
Physical Location & Seq
Forward (+)
20799392 .. 20822765
23374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG189200.1

Sequence Viewer

Length: 1461 bp
ATGGATACGGCTCTGCATGTGGCTGTGCGGTACGGTCATCTTGGAGTGGTGGTTATGTTAATGGAAGCCGATCCTGAACTGTGTTGTTTGACTAACAGGGCAGATGAATCTGTTTTGTTCTTGGCTGCTAGGAAGGGGTCTGCAGAGATTGCTCTTTATCTGTTGAACAAGTATCCGGTGTGTCCTTGTTTTCGAGGGACTAATGGTGTGACGGCTTTGCACGCCGCTGTAACTCGCAGCAACCTCACCAGCAAAGGCATTGTGAAGACTATGGTGTCCAAAAATCCTGAGATGATTAAAAACGTCGATGCACTTGGCTGGACACCGTTACATTATGCAGCATTTAGAGGGAACCTTGAAGCGACTAGAGTGCTAATGCAATGTGATAGTTCTGCATCTTACATCTTAGACAACTCTGGAATGTCAGCTCTCCATGTAGCATCCCATGCCGGCCACACCAAGATAATGAAAGAACTAATTCGGTGGCGACCTGATATTTGTGATCTGCTGAATGATAAAGGCCAAACGGCTTTACATGTGGCAGTATTAGGAGCACGAATTAATGTTGTGGAGTACATATTGAAGACGCCTAAGCTTGCAGCGATTATAAACGAAGCAGATAAAGATGGCAACACTCCTTTGCACTTGGCTGCCCTTCAGCAAAATGATAAAATCATCAAAATACTGTCAGGCAATCGTAGAGTGGACCTGATTGCTATTAACAAGGATTTCTTGAAGCCCATTGACATGTTTCTTGGTGAAAATATTGGTGAACAGACTAAAAGAGTGTGCTTTCTGAATCTTTTCAAGGAAACAATTCGCAGTGGTATGATTTTGCACAACCTGGGTTGCTCCGTTGGTGTGCCATTTTTCCAACAACAAATCAGTCGTGACTTCGACAAATTGGAACCACTAGAGAAGGACACAGACAAGATCAGAGAAAACCAGCTGCAAGCTCCTCCGGATGATCGTAATGCCTTAGAAAGAGATGACACCAAACTACTTGTTGCAACGCTCATTGCAACTGTCACGTTTGCAGCATCTTTCACCAGCGTGCCTGGAGGATTTAAAGACAACGGAATGCCTATTTTATATGACAAGGCCGCTTTCAAAGTGTTTCAATTCTTCAATGCAGTGTCCTTCTACATCTCAATTCTTGCAATCTATAACGAATCAACTCCGATAACTATGTTATCCATCCATCTACCCACTCCATCAATTCTGATTCAGTATTCAATTGGAGGAATGGTGGTTGCATTTTTTTCGGGCACGTTAGCGGTGCAGCCAAGACAGTACGATGGAAATTTGCTTGAATTTTTATTTGGTACAAGTTGGGTTGATATTCTGCTCGCGGCAATTTGTGGCATTTTATTAATGTTAGCCATCATCCCTGTGCTGAAGTCTCTTATTCAAATAAGCAGGGGAAAGAGGATCCACTGGCTCCGAAACCATGTAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

486

Amino Acids

53.78

Weight (kDa)

8.46

Isoelectric Point (pI)

27.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 94 6.8e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 86 - 161 4.6e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 144 - 193 3.7e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 168 - 230 3.8e-10 Ankyrin repeats (3 copies)
PGG PF13962 329 - 426 2.3e-18 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 608
AasI GACNNNNNNGTC 1 cut(s) 274
AccII CGCG 1 cut(s) 1352
AccIII TCCGGA 1 cut(s) 961
AciI CCGC 5 cut(s) 28, 225, 1104, 1277, 1352
AclWI GGATC 3 cut(s) 65, 1426, 1439
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 2 cut(s) 1303, 1313
AcuI CTGAAG 2 cut(s) 641, 1418
AcyI GRCGYC 1 cut(s) 587
AfaI GTAC 4 cut(s) 32, 575, 1295, 1327
AflIII ACRYGT 2 cut(s) 535, 747
AjnI CCWGG 2 cut(s) 843, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 1305, 1337
AleI CACNNNNGTG 1 cut(s) 1052
AluBI AGCT 4 cut(s) 428, 595, 949, 956
AluI AGCT 4 cut(s) 428, 595, 949, 956
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 1 cut(s) 1407
AlwI GGATC 3 cut(s) 65, 1426, 1439
Aor13HI TCCGGA 1 cut(s) 961
AoxI GGCC 3 cut(s) 451, 520, 1101
ApeKI GCWGC 8 cut(s) 125, 237, 338, 599, 650, 949, 1037, 1282
ApoI RAATTY 2 cut(s) 1303, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 480, 512
AseI ATTAAT 2 cut(s) 561, 1373
Asp700I GAANNNNTTC 3 cut(s) 477, 803, 816
AspS9I GGNCC 1 cut(s) 706
AsuHPI GGTGA 4 cut(s) 238, 770, 782, 1039
AvaII GGWCC 1 cut(s) 706
BaeGI GKGCMC 1 cut(s) 1271
BamHI GGATCC 1 cut(s) 1431
BbsI GAAGAC 2 cut(s) 272, 590
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 8 cut(s) 112, 249, 350, 611, 637, 936, 1049, 1294
BccI CCATC 6 cut(s) 620, 1205, 1209, 1222, 1292, 1391
BceAI ACGGC 3 cut(s) 24, 228, 543
BcgI CGANNNNNNTGC 4 cut(s) 352, 386, 1245, 1279
BciT130I CCWGG 2 cut(s) 845, 1059
BciVI GTATCC 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 1407
BfaI CTAG 3 cut(s) 129, 366, 914
BfmI CTRYAG 1 cut(s) 141
BfuI GTATCC 1 cut(s) 183
Bme1390I CCNGG 2 cut(s) 845, 1059
Bme18I GGWCC 1 cut(s) 706
BmgT120I GGNCC 1 cut(s) 706
BmiI GGNNCC 4 cut(s) 353, 909, 1433, 1442
BmrFI CCNGG 2 cut(s) 845, 1059
BmsI GCATC 4 cut(s) 298, 404, 449, 1049
BpiI GAAGAC 2 cut(s) 272, 590
BpmI CTGGAG 1 cut(s) 1080
Bpu10I CCTNAGC 1 cut(s) 591
BsaHI GRCGYC 1 cut(s) 587
BsaJI CCNNGG 1 cut(s) 844
BsaWI WCCGGW 2 cut(s) 175, 961
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 1233, 1263
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 1 cut(s) 1442
Bse3DI GCAATG 2 cut(s) 386, 1017
BseAI TCCGGA 1 cut(s) 961
BseBI CCWGG 2 cut(s) 845, 1059
BseDI CCNNGG 1 cut(s) 844
BseGI GGATG 4 cut(s) 440, 970, 1197, 1386
BseMI GCAATG 2 cut(s) 386, 1017
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 1442
BseRI GAGGAG 1 cut(s) 948
BseSI GKGCMC 1 cut(s) 1271
BseXI GCAGC 8 cut(s) 112, 249, 350, 611, 637, 936, 1049, 1294
BsgI GTGCAG 1 cut(s) 1301
Bsh1236I CGCG 1 cut(s) 1352
BshFI GGCC 3 cut(s) 453, 522, 1103
BsiHKAI GWGCWC 1 cut(s) 556
BsiSI CCGG 3 cut(s) 176, 450, 962
BslFI GGGAC 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 1407
BsmFI GGGAC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 1086
BsnI GGCC 3 cut(s) 453, 522, 1103
Bsp1286I GDGCHC 2 cut(s) 556, 1271
Bsp13I TCCGGA 1 cut(s) 961
Bsp143I GATC 5 cut(s) 70, 502, 933, 967, 1431
BspACI CCGC 5 cut(s) 28, 225, 1104, 1277, 1352
BspANI GGCC 3 cut(s) 453, 522, 1103
BspCNI CTCAG 1 cut(s) 280
BspEI TCCGGA 1 cut(s) 961
BspFNI CGCG 1 cut(s) 1352
BspLI GGNNCC 4 cut(s) 353, 909, 1433, 1442
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 3 cut(s) 65, 1426, 1439
BsrDI GCAATG 2 cut(s) 386, 1017
BsrFI RCCGGY 1 cut(s) 449
BsrI ACTGG 1 cut(s) 1442
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 1 cut(s) 844
BssMI GATC 5 cut(s) 70, 502, 933, 967, 1431
BssNI GRCGYC 1 cut(s) 587
Bst2UI CCWGG 2 cut(s) 845, 1059
Bst4CI ACNGT 6 cut(s) 35, 81, 327, 687, 1027, 1293
BstACI GRCGYC 1 cut(s) 587
BstAPI GCANNNNNTGC 2 cut(s) 149, 446
BstC8I GCNNGC 6 cut(s) 222, 451, 597, 954, 1055, 1350
BstDEI CTNAG 4 cut(s) 288, 406, 591, 979
BstF5I GGATG 4 cut(s) 440, 970, 1197, 1386
BstFNI CGCG 1 cut(s) 1352
BstKTI GATC 5 cut(s) 73, 505, 936, 970, 1434
BstMAI GTCTC 1 cut(s) 1407
BstMBI GATC 5 cut(s) 70, 502, 933, 967, 1431
BstMWI GCNNNNNNNGC 3 cut(s) 149, 221, 446
BstNI CCWGG 2 cut(s) 845, 1059
BstNSI RCATGY 3 cut(s) 20, 539, 751
BstSCI CCNGG 2 cut(s) 843, 1057
BstSFI CTRYAG 1 cut(s) 141
BstSLI GKGCMC 1 cut(s) 1271
BstUI CGCG 1 cut(s) 1352
BstV1I GCAGC 8 cut(s) 112, 249, 350, 611, 637, 936, 1049, 1294
BstV2I GAAGAC 2 cut(s) 272, 590
BstX2I RGATCY 1 cut(s) 1431
BstYI RGATCY 1 cut(s) 1431
BsuI GTATCC 1 cut(s) 183
BsuRI GGCC 3 cut(s) 453, 522, 1103
BtsCI GGATG 4 cut(s) 440, 970, 1197, 1386
BtsI GCAGTG 2 cut(s) 829, 1140
BtsIMutI CAGTG 3 cut(s) 829, 1140, 1435
Cac8I GCNNGC 6 cut(s) 222, 451, 597, 954, 1055, 1350
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 706
CseI GACGC 1 cut(s) 595
Csp6I GTAC 4 cut(s) 31, 574, 1294, 1326
CviAII CATG 6 cut(s) 17, 434, 446, 536, 748, 1451
CviQI GTAC 4 cut(s) 31, 574, 1294, 1326
DdeI CTNAG 4 cut(s) 288, 406, 591, 979
DpnI GATC 5 cut(s) 72, 504, 935, 969, 1433
DpnII GATC 5 cut(s) 70, 502, 933, 967, 1431
DraI TTTAAA 1 cut(s) 1069
DrdI GACNNNNNNGTC 1 cut(s) 274
DseDI GACNNNNNNGTC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 706
Eco57I CTGAAG 2 cut(s) 641, 1418
EcoRII CCWGG 2 cut(s) 843, 1057
FaeI CATG 6 cut(s) 20, 437, 449, 539, 751, 1454
FalI AAGNNNNNCTT 2 cut(s) 716, 748
FaqI GGGAC 1 cut(s) 211
FatI CATG 6 cut(s) 16, 433, 445, 535, 747, 1450
FokI GGATG 4 cut(s) 427, 977, 1184, 1373
FspBI CTAG 3 cut(s) 129, 366, 914
GsuI CTGGAG 1 cut(s) 1080
HaeIII GGCC 3 cut(s) 453, 522, 1103
HapII CCGG 3 cut(s) 176, 450, 962
HgaI GACGC 1 cut(s) 595
Hin1I GRCGYC 1 cut(s) 587
Hin1II CATG 6 cut(s) 20, 437, 449, 539, 751, 1454
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 4 cut(s) 107, 799, 1172, 1225
HpaII CCGG 3 cut(s) 176, 450, 962
HphI GGTGA 4 cut(s) 238, 770, 782, 1039
Hpy166II GTNNAC 2 cut(s) 706, 773
Hpy188I TCNGA 5 cut(s) 798, 938, 1182, 1224, 1445
Hpy188III TCNNGA 6 cut(s) 74, 287, 417, 733, 890, 962
Hpy8I GTNNAC 2 cut(s) 706, 773
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 4 cut(s) 127, 665, 913, 1150
HpyCH4III ACNGT 6 cut(s) 35, 81, 327, 687, 1027, 1293
HpyCH4IV ACGT 3 cut(s) 303, 1031, 1271
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 221, 446
HpyF3I CTNAG 4 cut(s) 288, 406, 591, 979
HpySE526I ACGT 3 cut(s) 303, 1031, 1271
Hsp92I GRCGYC 1 cut(s) 587
Hsp92II CATG 6 cut(s) 20, 437, 449, 539, 751, 1454
Kpn2I TCCGGA 1 cut(s) 961
KroI GCCGGC 1 cut(s) 449
KroNI GCCGGC 1 cut(s) 451
Kzo9I GATC 5 cut(s) 70, 502, 933, 967, 1431
LmnI GCTCC 4 cut(s) 551, 857, 961, 1446
Lsp1109I GCAGC 8 cut(s) 112, 249, 350, 611, 637, 936, 1049, 1294
LweI GCATC 4 cut(s) 298, 404, 449, 1049
MaeI CTAG 3 cut(s) 129, 366, 914
MaeII ACGT 3 cut(s) 303, 1031, 1271
MaeIII GTNAC 5 cut(s) 208, 229, 327, 890, 1027
MalI GATC 5 cut(s) 72, 504, 935, 969, 1433
MboI GATC 5 cut(s) 70, 502, 933, 967, 1431
MboII GAAGA 3 cut(s) 277, 595, 1117
MfeI CAATTG 1 cut(s) 1236
MflI RGATCY 1 cut(s) 1431
MhlI GDGCHC 2 cut(s) 556, 1271
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 7 cut(s) 188, 254, 341, 969, 1055, 1235, 1422
MroI TCCGGA 1 cut(s) 961
MroNI GCCGGC 1 cut(s) 449
MroXI GAANNNNTTC 3 cut(s) 477, 803, 816
MseI TTAA 7 cut(s) 59, 297, 561, 720, 1068, 1373, 1459
MslI CAYNNNNRTG 3 cut(s) 746, 1052, 1391
MspA1I CMGCKG 2 cut(s) 227, 949
MspI CCGG 3 cut(s) 176, 450, 962
MspR9I CCNGG 2 cut(s) 845, 1059
MunI CAATTG 1 cut(s) 1236
Mva1269I GAATGC 1 cut(s) 1086
MvaI CCWGG 2 cut(s) 845, 1059
MvnI CGCG 1 cut(s) 1352
MwoI GCNNNNNNNGC 3 cut(s) 149, 221, 446
NaeI GCCGGC 1 cut(s) 451
NdeII GATC 5 cut(s) 70, 502, 933, 967, 1431
NgoMIV GCCGGC 1 cut(s) 449
NlaIII CATG 6 cut(s) 20, 437, 449, 539, 751, 1454
NlaIV GGNNCC 4 cut(s) 353, 909, 1433, 1442
NmuCI GTSAC 3 cut(s) 208, 890, 1027
NspI RCATGY 3 cut(s) 20, 539, 751
OliI CACNNNNGTG 1 cut(s) 1052
PciI ACATGT 2 cut(s) 535, 747
PctI GAATGC 1 cut(s) 1086
PdiI GCCGGC 1 cut(s) 451
PdmI GAANNNNTTC 3 cut(s) 477, 803, 816
PfeI GAWTC 4 cut(s) 107, 799, 1172, 1225
PscI ACATGT 2 cut(s) 535, 747
PshBI ATTAAT 2 cut(s) 561, 1373
PsiI TTATAA 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 843, 1057
PspGI CCWGG 2 cut(s) 843, 1057
PspN4I GGNNCC 4 cut(s) 353, 909, 1433, 1442
PspPI GGNCC 1 cut(s) 706
PstI CTGCAG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 1431
PvuII CAGCTG 1 cut(s) 949
RsaI GTAC 4 cut(s) 32, 575, 1295, 1327
RsaNI GTAC 4 cut(s) 31, 574, 1294, 1326
RseI CAYNNNNRTG 3 cut(s) 746, 1052, 1391
SaqAI TTAA 7 cut(s) 59, 297, 561, 720, 1068, 1373, 1459
Sau3AI GATC 5 cut(s) 70, 502, 933, 967, 1431
Sau96I GGNCC 1 cut(s) 706
ScrFI CCNGG 2 cut(s) 845, 1059
SduI GDGCHC 2 cut(s) 556, 1271
SfaNI GCATC 4 cut(s) 298, 404, 449, 1049
SfcI CTRYAG 1 cut(s) 141
SinI GGWCC 1 cut(s) 706
SmiMI CAYNNNNRTG 3 cut(s) 746, 1052, 1391
SsiI CCGC 5 cut(s) 28, 225, 1104, 1277, 1352
SspI AATATT 1 cut(s) 766
SspMI CTAG 3 cut(s) 129, 366, 914
StyD4I CCNGG 2 cut(s) 843, 1057
TaaI ACNGT 6 cut(s) 35, 81, 327, 687, 1027, 1293
TaiI ACGT 3 cut(s) 306, 1034, 1274
TaqI TCGA 3 cut(s) 193, 306, 897
TatI WGTACW 1 cut(s) 573
TauI GCSGC 3 cut(s) 227, 1106, 1355
TfiI GAWTC 4 cut(s) 107, 799, 1172, 1225
Tru1I TTAA 7 cut(s) 59, 297, 561, 720, 1068, 1373, 1459
Tru9I TTAA 7 cut(s) 59, 297, 561, 720, 1068, 1373, 1459
TscAI CASTG 3 cut(s) 829, 1140, 1442
TseFI GTSAC 3 cut(s) 208, 890, 1027
TseI GCWGC 8 cut(s) 125, 237, 338, 599, 650, 949, 1037, 1282
Tsp45I GTSAC 3 cut(s) 208, 890, 1027
TspDTI ATGAA 2 cut(s) 120, 482
TspGWI ACGGA 2 cut(s) 844, 1092
TspRI CASTG 3 cut(s) 829, 1140, 1442
VpaK11BI GGWCC 1 cut(s) 706
VspI ATTAAT 2 cut(s) 561, 1373
XapI RAATTY 2 cut(s) 1303, 1313
XceI RCATGY 3 cut(s) 20, 539, 751
XmnI GAANNNNTTC 3 cut(s) 477, 803, 816
XspI CTAG 3 cut(s) 129, 366, 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.