Prupe.4G144500_v2.0.a1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000319
Physical Location & Seq
Forward (+)
8299907 .. 8302100
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G144500.1

Sequence Viewer

Length: 1629 bp
ATGGAGCCTTCTTTGCATGAGGCTGCAAGGTCGGGTGAAGTTGGGTTCTTGAGAAGAATGAAAGACGGTGATGTATCAATTGACCTTCTTCTTCAGAAAACACCTAAAGGAAACAATATTCTTCACATTGCGGCTGAATTCAAGCAAATAGAGTTCTTCAAAGAACTTGATGATCAATCTTCGTTGTTTTGGGCTACCAACGAGAAGGGAGACACTCCCCTGCATGTTGCTGCCAGAGTAGGCTGGGATCAAGTAGTAAAGTTCCTCACTGAGCACACAAAGAAGATGCTACATGTACAAGAAGCCGATGAGGAAAGCAGATCAACCGATAGCGAAGCTCATAAGCAGCTACTTCAAATGACTAATTTGCAAAGGGATACTGCATTGCATGTTGCTTCCAGATATGGTCATAGTGGAGTGGTGATTCAGCTAATGCAAGCCAATCCAGAATTGTGTTGTTACACTAATAGCGCCAACGAGTCACCTTTGTTCATTGCTACTAGCAGGGGCTTTCGACACATTATTCTTCATCTTTTAAACGAGTCTCCAATATGTCCTTCTTTTCAGGGTATTAATGGTGTCACAGCTTTGCATGCCGCCGCAACTCACACAACCAAAGATTCCAAAGGCATTGTGAAACTAATGGTGTCCAGAAATCCTGAAATAGTTAAAGAAGCTGATGCAATTGGCCGGACTCCCTTACACTATGCGGCATTGAGAGGAAACCTTGAAGCAACTCGAGTGCTGACGCAATGCGATAGTTCTGTCTCTTACATCTTGCACAAATCTGGCATGTCAGCTCTCCATGTCGCCGCCTATGCAGGGCAGGCCAAAGGCCAAACGGTTCTTCATGCTGCAGTTTTAGGGGGGCAAGTAGACGTTGTCAAGTATATATTGAAGACTCCCCAACTTGCAGGATTTATAAATGAAGCAGATAAAGATGGAAACACTGCTTTGCATTTGGCTGCCATTCATCAAAATATTGAAATTATAGGAACTTTTGCAAGTGACAGTAGAGTGGATATGACAGCTACCAATAAGGAATTCTCAAAGGCGGTTGACATTTTTGTTGTGGACGACGGAAAATTGGATCTCATTCGTATTGAACTTGTAAGTCACCTGGGGAAGTCCATTGGAGTGCCATTTTTCCAACAACAAATTTCCCATGATTTCATGAATTTGGAATCTCCAAAGCACAAAGATACACCTACCATATCAGCCGCTATAGGAAAAAAACATAGAAAAGTTGTAGTAGCAATGCTTATTGCAACCGTCACATTTGCCGCAGCTTTCACCATGCCGGGAGGACTTAAAAGCGACGGAACTGCGGTGTTATGCGGAGAGGCATCATTCCAAGTGTTCTTGCTATTTGATGCCTTCTCCTTCTTCTTGTCAATCCTCATGGTGTTTCATCATTTCATGGTGGCAACTGTGTCAGGTGTTCTTGTAGCTGCACCTTCAATTCTCATTCAGTATTCAATTGGAGGAATGCTCGTAGCTTTTGCTTCGGGCATGCTTCTAGTGCTGCCCAAACACAGTCCGCTGGGCATTTTGCTTAGAATTGTGCCTGTATCAAGCAGCAGTCGTGAAAAGACTAGCCGCAAATGGTTCCTGACGCGCATAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

543

Amino Acids

59.21

Weight (kDa)

7.79

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 923
AccI GTMKAC 1 cut(s) 876
AccII CGCG 1 cut(s) 1618
AclWI GGATC 2 cut(s) 255, 1098
AcoI YGGCCR 1 cut(s) 688
AcsI RAATTY 4 cut(s) 137, 1043, 1158, 1177
AcuI CTGAAG 1 cut(s) 77
AfaI GTAC 1 cut(s) 297
AfiI CCNNNNNNNGG 1 cut(s) 822
AflIII ACRYGT 1 cut(s) 292
AgsI TTSAA 9 cut(s) 142, 160, 356, 731, 898, 986, 1106, 1461, 1479
AjnI CCWGG 1 cut(s) 1119
AjuI GAANNNNNNNTTGG 6 cut(s) 541, 573, 831, 863, 1028, 1060
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 3 cut(s) 204, 549, 772
AlwI GGATC 2 cut(s) 255, 1098
Ama87I CYCGRG 1 cut(s) 738
AoxI GGCC 3 cut(s) 688, 828, 835
ApeKI GCWGC 9 cut(s) 23, 230, 346, 854, 965, 1286, 1451, 1525, 1578
ApoI RAATTY 4 cut(s) 137, 1043, 1158, 1177
AseI ATTAAT 1 cut(s) 573
AspLEI GCGC 2 cut(s) 473, 1620
AsuC2I CCSGG 1 cut(s) 1302
AsuHPI GGTGA 6 cut(s) 47, 80, 433, 474, 1109, 1285
AvaI CYCGRG 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 9 cut(s) 10, 217, 358, 841, 952, 1298, 1438, 1512, 1590
BccI CCATC 1 cut(s) 935
BcgI CGANNNNNNTGC 2 cut(s) 1307, 1341
BciT130I CCWGG 1 cut(s) 1121
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 172
BcnI CCSGG 1 cut(s) 1302
BcoDI GTCTC 3 cut(s) 204, 549, 772
BfaI CTAG 3 cut(s) 501, 1520, 1596
BfmI CTRYAG 2 cut(s) 855, 1224
BfoI RGCGCY 1 cut(s) 474
BfuI GTATCC 1 cut(s) 370
Bme1390I CCNGG 2 cut(s) 1121, 1302
BmeT110I CYCGRG 1 cut(s) 738
BmiI GGNNCC 2 cut(s) 6, 1610
BmrFI CCNGG 2 cut(s) 1121, 1302
BmsI GCATC 4 cut(s) 276, 670, 1355, 1363
BpiI GAAGAC 1 cut(s) 905
BplI GAGNNNNNCTC 2 cut(s) 1476, 1508
BpuEI CTTGAG 1 cut(s) 70
BpuMI CCSGG 1 cut(s) 1302
BsaJI CCNNGG 1 cut(s) 1120
Bsc4I CCNNNNNNNGG 1 cut(s) 822
Bse3DI GCAATG 5 cut(s) 126, 383, 492, 758, 1263
BseBI CCWGG 1 cut(s) 1121
BseDI CCNNGG 1 cut(s) 1120
BseLI CCNNNNNNNGG 1 cut(s) 822
BseMI GCAATG 5 cut(s) 126, 383, 492, 758, 1263
BseMII CTCAG 1 cut(s) 261
BseXI GCAGC 9 cut(s) 10, 217, 358, 841, 952, 1298, 1438, 1512, 1590
BseYI CCCAGC 2 cut(s) 243, 1543
BsgI GTGCAG 1 cut(s) 1437
Bsh1236I CGCG 1 cut(s) 1618
BshFI GGCC 3 cut(s) 690, 830, 837
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 738
BsiSI CCGG 2 cut(s) 691, 1301
BslI CCNNNNNNNGG 1 cut(s) 822
BsmAI GTCTC 3 cut(s) 204, 549, 772
BsmI GAATGC 1 cut(s) 1494
BsnI GGCC 3 cut(s) 690, 830, 837
BsoBI CYCGRG 1 cut(s) 738
Bsp1286I GDGCHC 1 cut(s) 276
Bsp1407I TGTACA 1 cut(s) 295
Bsp143I GATC 4 cut(s) 172, 247, 320, 1090
BspANI GGCC 3 cut(s) 690, 830, 837
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 1 cut(s) 1618
BspHI TCATGA 1 cut(s) 1173
BspLI GGNNCC 2 cut(s) 6, 1610
BspMAI CTGCAG 1 cut(s) 859
BspPI GGATC 2 cut(s) 255, 1098
BsrDI GCAATG 5 cut(s) 126, 383, 492, 758, 1263
BsrGI TGTACA 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 1120
BssMI GATC 4 cut(s) 172, 247, 320, 1090
Bst2UI CCWGG 1 cut(s) 1121
Bst4CI ACNGT 6 cut(s) 68, 844, 1013, 1273, 1432, 1538
BstAUI TGTACA 1 cut(s) 295
BstC8I GCNNGC 4 cut(s) 438, 594, 828, 1514
BstDEI CTNAG 2 cut(s) 270, 1556
BstFNI CGCG 1 cut(s) 1618
BstH2I RGCGCY 1 cut(s) 474
BstHHI GCGC 2 cut(s) 473, 1620
BstKTI GATC 4 cut(s) 175, 250, 323, 1093
BstMAI GTCTC 3 cut(s) 204, 549, 772
BstMBI GATC 4 cut(s) 172, 247, 320, 1090
BstMWI GCNNNNNNNGC 5 cut(s) 13, 593, 818, 827, 1522
BstNI CCWGG 1 cut(s) 1121
BstNSI RCATGY 6 cut(s) 227, 296, 392, 596, 796, 1516
BstSCI CCNGG 2 cut(s) 1119, 1300
BstSFI CTRYAG 2 cut(s) 855, 1224
BstUI CGCG 1 cut(s) 1618
BstV1I GCAGC 9 cut(s) 10, 217, 358, 841, 952, 1298, 1438, 1512, 1590
BstV2I GAAGAC 1 cut(s) 905
BstX2I RGATCY 1 cut(s) 1090
BstYI RGATCY 1 cut(s) 1090
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 3 cut(s) 690, 830, 837
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 2 cut(s) 267, 948
Cac8I GCNNGC 4 cut(s) 438, 594, 828, 1514
CciI TCATGA 1 cut(s) 1173
CfoI GCGC 2 cut(s) 473, 1620
CseI GACGC 2 cut(s) 757, 1624
Csp6I GTAC 1 cut(s) 296
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 2 cut(s) 270, 1556
DpnI GATC 4 cut(s) 174, 249, 322, 1092
DpnII GATC 4 cut(s) 172, 247, 320, 1090
DraI TTTAAA 1 cut(s) 537
EaeI YGGCCR 1 cut(s) 688
Eco57I CTGAAG 1 cut(s) 77
Eco88I CYCGRG 1 cut(s) 738
EcoRI GAATTC 2 cut(s) 137, 1043
EcoRII CCWGG 1 cut(s) 1119
FbaI TGATCA 1 cut(s) 172
FblI GTMKAC 1 cut(s) 876
FspBI CTAG 3 cut(s) 501, 1520, 1596
GlaI GCGC 2 cut(s) 472, 1619
GsaI CCCAGC 2 cut(s) 247, 1547
HaeII RGCGCY 1 cut(s) 474
HaeIII GGCC 3 cut(s) 690, 830, 837
HapII CCGG 2 cut(s) 691, 1301
HgaI GACGC 2 cut(s) 757, 1624
HhaI GCGC 2 cut(s) 473, 1620
Hin6I GCGC 2 cut(s) 471, 1618
HinP1I GCGC 2 cut(s) 471, 1618
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HinfI GANTC 7 cut(s) 424, 479, 542, 620, 694, 901, 1184
HpaII CCGG 2 cut(s) 691, 1301
HphI GGTGA 6 cut(s) 47, 80, 433, 474, 1109, 1285
Hpy166II GTNNAC 3 cut(s) 877, 1060, 1075
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 8 cut(s) 49, 399, 446, 651, 659, 1174, 1586, 1612
Hpy8I GTNNAC 3 cut(s) 877, 1060, 1075
Hpy99I CGWCG 2 cut(s) 1082, 1322
HpyAV CCTTC 7 cut(s) 18, 95, 199, 567, 1387, 1393, 1467
HpyCH4III ACNGT 6 cut(s) 68, 844, 1013, 1273, 1432, 1538
HpyCH4IV ACGT 1 cut(s) 879
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 593, 818, 827, 1522
HpyF3I CTNAG 2 cut(s) 270, 1556
HpySE526I ACGT 1 cut(s) 879
HspAI GCGC 2 cut(s) 471, 1618
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 4 cut(s) 172, 247, 320, 1090
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 9 cut(s) 10, 217, 358, 841, 952, 1298, 1438, 1512, 1590
LweI GCATC 4 cut(s) 276, 670, 1355, 1363
MaeI CTAG 3 cut(s) 501, 1520, 1596
MaeII ACGT 1 cut(s) 879
MaeIII GTNAC 6 cut(s) 458, 480, 580, 1007, 1115, 1273
MalI GATC 4 cut(s) 174, 249, 322, 1092
MboI GATC 4 cut(s) 172, 247, 320, 1090
MfeI CAATTG 3 cut(s) 78, 684, 1479
MflI RGATCY 1 cut(s) 1090
MhlI GDGCHC 1 cut(s) 276
MlyI GAGTC 4 cut(s) 488, 551, 688, 895
MmeI TCCRAC 1 cut(s) 1174
MnlI CCTC 8 cut(s) 13, 275, 304, 713, 1298, 1336, 1409, 1478
MseI TTAA 4 cut(s) 536, 573, 669, 1311
MslI CAYNNNNRTG 1 cut(s) 1136
MspA1I CMGCKG 1 cut(s) 1543
MspI CCGG 2 cut(s) 691, 1301
MspR9I CCNGG 2 cut(s) 1121, 1302
MunI CAATTG 3 cut(s) 78, 684, 1479
Mva1269I GAATGC 1 cut(s) 1494
MvaI CCWGG 1 cut(s) 1121
MvnI CGCG 1 cut(s) 1618
MwoI GCNNNNNNNGC 5 cut(s) 13, 593, 818, 827, 1522
NciI CCSGG 1 cut(s) 1302
NdeII GATC 4 cut(s) 172, 247, 320, 1090
NlaIV GGNNCC 2 cut(s) 6, 1610
NmuCI GTSAC 5 cut(s) 480, 580, 1007, 1115, 1273
NspI RCATGY 6 cut(s) 227, 296, 392, 596, 796, 1516
PaeI GCATGC 2 cut(s) 596, 1516
PaeR7I CTCGAG 1 cut(s) 738
PagI TCATGA 1 cut(s) 1173
PciI ACATGT 1 cut(s) 292
PctI GAATGC 1 cut(s) 1494
PfeI GAWTC 3 cut(s) 424, 620, 1184
PflFI GACNNNGTC 1 cut(s) 881
PleI GAGTC 4 cut(s) 487, 550, 688, 895
PpsI GAGTC 4 cut(s) 487, 550, 688, 895
PscI ACATGT 1 cut(s) 292
PshBI ATTAAT 1 cut(s) 573
PsiI TTATAA 1 cut(s) 923
Psp6I CCWGG 1 cut(s) 1119
PspFI CCCAGC 2 cut(s) 243, 1543
PspGI CCWGG 1 cut(s) 1119
PspN4I GGNNCC 2 cut(s) 6, 1610
PspXI VCTCGAGB 1 cut(s) 738
PstI CTGCAG 1 cut(s) 859
PsuI RGATCY 1 cut(s) 1090
PsyI GACNNNGTC 1 cut(s) 881
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
RseI CAYNNNNRTG 1 cut(s) 1136
SaqAI TTAA 4 cut(s) 536, 573, 669, 1311
Sau3AI GATC 4 cut(s) 172, 247, 320, 1090
SchI GAGTC 4 cut(s) 488, 551, 688, 895
ScrFI CCNGG 2 cut(s) 1121, 1302
SduI GDGCHC 1 cut(s) 276
SfaNI GCATC 4 cut(s) 276, 670, 1355, 1363
SfcI CTRYAG 2 cut(s) 855, 1224
Sfr274I CTCGAG 1 cut(s) 738
SlaI CTCGAG 1 cut(s) 738
SmiMI CAYNNNNRTG 1 cut(s) 1136
SmlI CTYRAG 2 cut(s) 49, 738
SmoI CTYRAG 2 cut(s) 49, 738
SphI GCATGC 2 cut(s) 596, 1516
SspI AATATT 2 cut(s) 118, 982
SspMI CTAG 3 cut(s) 501, 1520, 1596
StyD4I CCNGG 2 cut(s) 1119, 1300
TaaI ACNGT 6 cut(s) 68, 844, 1013, 1273, 1432, 1538
TaiI ACGT 1 cut(s) 882
TaqI TCGA 2 cut(s) 514, 739
TatI WGTACW 1 cut(s) 295
TauI GCSGC 8 cut(s) 134, 599, 602, 713, 815, 1223, 1286, 1602
TfiI GAWTC 3 cut(s) 424, 620, 1184
Tru1I TTAA 4 cut(s) 536, 573, 669, 1311
Tru9I TTAA 4 cut(s) 536, 573, 669, 1311
TscAI CASTG 2 cut(s) 274, 955
TseFI GTSAC 5 cut(s) 480, 580, 1007, 1115, 1273
TseI GCWGC 9 cut(s) 23, 230, 346, 854, 965, 1286, 1451, 1525, 1578
Tsp45I GTSAC 5 cut(s) 480, 580, 1007, 1115, 1273
TspGWI ACGGA 2 cut(s) 1095, 1335
TspRI CASTG 2 cut(s) 274, 955
Tth111I GACNNNGTC 1 cut(s) 881
VspI ATTAAT 1 cut(s) 573
XapI RAATTY 4 cut(s) 137, 1043, 1158, 1177
XceI RCATGY 6 cut(s) 227, 296, 392, 596, 796, 1516
XhoI CTCGAG 1 cut(s) 738
XmiI GTMKAC 1 cut(s) 876
XspI CTAG 3 cut(s) 501, 1520, 1596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.