MD05G1212300.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000319
Physical Location & Seq
Reverse (-)
34353548 .. 34355894
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1212300.v1.1.491

Sequence Viewer

Length: 1839 bp
ATGGATCCTTCTCTGTATGAAGCAGCTGCAACTGGTGATGTTGGCTTCTTCGAAGGACATCCAACAGCTGATCTCTTTCAGAAAACACCTAAAGAGAACAATATTCTTCACATTGCAGCTGAATTCAAGCAAATAAAGTTTTTCAAAAAAGTGAAAAAAAATCTCGAATCACCCCGGTTTTGGGCCACCAACAAGAATGGCGAAACTCCTCTACATGTTGCTGCGAGAGTAGGATGTGGTGAAGTAGTAACGTTCCTCATTGAACACACAAAATCGGAACCTATGGAAGTAGCTGATCTTGATCCAGAAAAGGAACCAACTGAAGATGAAGCTTACAAAAAGCTACTCCGAATGCCTAATTTGGAAATGGATACAGCACTGCATGTTGCTGTCCGATACAATCATGCTGGAGTAGCGAAGATTTTGCTGAATGCTGATCCTGAATTGTGTTGTTCATTTTACAGCACCAAGGAGTCACCCCTGTTCCTAGCTGTTAGAGCCGGGTCTACCAGCATTGCTGATTATATTTTAAAAAAGACTCCGGATCATAAATCTCTTTCTTTTCAGGGAACAAATGGTGTGACCGCTTTGCACGCAGCAGCCACTCGTATATTCTTTGCCAAGAAAGGAATTTTGCAATCTATGGTTACCAAAAATCCTGGGATGATCGAAGTTCGTGATGAAATAGGGTGGACTCCATTACACTATGCATCACTGAGAGGGAACCTTAAAGCAGCTCAACTACTGATTCAAAATTCGAAAACTTCCGAATTTGCTTGTTACATCGAAGATGAACTTGGCATGTCAGCTCTCCACGTCGCGGCATATGCAGGGCACACCCAAATAATTGAAGAGTTGATTCGACGTTGTCCTGATATTTGCGATTCGGTCAATCACAAGGGCCAAACAGCTCTACATGCAGCAGTTTTAGGCGAAAAAATAAATGTAGTGAAGTACGTTTTGAAGACGCCTAAGCTTGCGAGACTTATAAACGAAGCAGATAATGATGGAAACACTCCTTGGCATCTAGCTGCTATTTGCAAAAATAGTAAAATTGTTGCTATTTTAAGGAGAGACAGTAGGTTGAACAGAACTGCTATCAATAAAAAAAAATTGCAAGTTAGCGACATTTTGCTCGCCGAGAACACGGGAAGAAAGGAAATCATAAAAAGTCGCAATGTTTTGAACTACCTGGGAAGAGACATTGTCGTTCCCGTGCCATATTTTCAACAAAAAATCAAGCAGGAGTTCGAGAAATTTGGATCCCAAGGGGAGAGCAGTACTTCTGAGACACCAGTCAATACTACAAATACGAGAGAAAACTTGCAGGCTTATTCGGATCAGACCTTCAAAAGAAATGATTCGAAACTTCTGGTAGCGACGGTCATTGCAACCGTTACATTTACGGCCGTTATGAACCCGCCTGGAGGGTATAAAGACGATGGAACACCAGTTTTAGAAAAAATAGCAGCTTACAAAGTATTTTGGTTTTTTAACACGGTATCCTTCGCTCTCTCAGTGTTGGCGATATTTAATGAAACCACTCCGATACCTGTCCTATCTACCCATCTACCCACACCTGCGAGTCTTATTAGTTATTCCATTGCTGGAATGGTGATTGCATTTAATGCAGCCCAGACGGCAGTGGAGCACAAGCGGCCCGGGCAAAGTATGGTTAAATATTATTTGTTCGGCGACGACACCGGTGTTACTACTATCCTCAATCTTATTGGTAGCGTAATTGTAGGCGCTCTAGCTGGCATCGCTTTTACGAAGTCTCTATTTCAAATAATCATGGGGAAAGGAAGGATCCACGACCTCCAGAACCACATAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

613

Amino Acids

67.58

Weight (kDa)

8.76

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 24 - 90 6e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 113 - 182 3.9e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 127 - 201 3.8e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 161 - 252 1.8e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 207 - 288 3.7e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 262 - 322 5.8e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 271 - 320 1.9e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 295 - 363 9.3e-09 Ankyrin repeats (3 copies)
PGG PF13962 451 - 548 3.8e-19 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 989
AarI CACCTGC 1 cut(s) 1588
Acc36I ACCTGC 1 cut(s) 1588
AccI GTMKAC 1 cut(s) 506
AccII CGCG 1 cut(s) 821
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 4 cut(s) 585, 821, 1421, 1657
AclI AACGTT 1 cut(s) 251
AclWI GGATC 9 cut(s) 12, 296, 431, 552, 1257, 1270, 1347, 1804, 1817
AcoI YGGCCR 1 cut(s) 1407
AcsI RAATTY 5 cut(s) 122, 630, 754, 770, 1256
AcuI CTGAAG 1 cut(s) 342
AcyI GRCGYC 1 cut(s) 968
AfaI GTAC 2 cut(s) 956, 1282
AfiI CCNNNNNNNGG 4 cut(s) 180, 181, 820, 1427
AflIII ACRYGT 1 cut(s) 214
AgeI ACCGGT 1 cut(s) 1703
AjiI CACGTC 1 cut(s) 817
AjnI CCWGG 3 cut(s) 658, 1191, 1423
AjuI GAANNNNNNNTTGG 4 cut(s) 780, 812, 1003, 1035
Alw21I GWGCWC 1 cut(s) 1653
Alw26I GTCTC 5 cut(s) 976, 1068, 1194, 1283, 1782
AlwI GGATC 9 cut(s) 12, 296, 431, 552, 1257, 1270, 1347, 1804, 1817
Ama87I CYCGRG 1 cut(s) 1661
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 4 cut(s) 183, 901, 1407, 1658
ApoI RAATTY 5 cut(s) 122, 630, 754, 770, 1256
AsiGI ACCGGT 1 cut(s) 1703
Asp700I GAANNNNTTC 1 cut(s) 1360
AspLEI GCGC 1 cut(s) 1751
AspS9I GGNCC 3 cut(s) 183, 901, 1659
AsuC2I CCSGG 4 cut(s) 175, 502, 1662, 1663
AsuHPI GGTGA 5 cut(s) 47, 162, 251, 468, 1627
AsuII TTCGAA 3 cut(s) 51, 758, 1364
AvaI CYCGRG 1 cut(s) 1661
BaeGI GKGCMC 1 cut(s) 837
BamHI GGATCC 3 cut(s) 4, 1262, 1809
BbsI GAAGAC 1 cut(s) 971
Bbv12I GWGCWC 1 cut(s) 1653
BccI CCATC 3 cut(s) 1001, 1436, 1575
BceAI ACGGC 3 cut(s) 1394, 1422, 1656
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 3 cut(s) 660, 1193, 1425
BciVI GTATCC 2 cut(s) 364, 1513
BcnI CCSGG 4 cut(s) 175, 502, 1662, 1663
BcoDI GTCTC 5 cut(s) 976, 1068, 1194, 1283, 1782
BfaI CTAG 3 cut(s) 488, 1028, 1754
BfoI RGCGCY 1 cut(s) 1752
BfuAI ACCTGC 1 cut(s) 1588
BfuI GTATCC 2 cut(s) 364, 1513
BglI GCCNNNNNGGC 1 cut(s) 1640
BmcAI AGTACT 1 cut(s) 1282
Bme1390I CCNGG 7 cut(s) 175, 502, 660, 1193, 1425, 1662, 1663
BmeT110I CYCGRG 1 cut(s) 1661
BmgBI CACGTC 1 cut(s) 817
BmgT120I GGNCC 3 cut(s) 183, 901, 1659
BmiI GGNNCC 6 cut(s) 6, 279, 315, 725, 1264, 1811
BmrFI CCNGG 7 cut(s) 175, 502, 660, 1193, 1425, 1662, 1663
BmsI GCATC 3 cut(s) 719, 1033, 1770
BoxI GACNNNNGTC 1 cut(s) 1295
BpiI GAAGAC 1 cut(s) 971
BpmI CTGGAG 3 cut(s) 429, 1446, 1805
Bpu10I CCTNAGC 1 cut(s) 972
Bpu14I TTCGAA 3 cut(s) 51, 758, 1364
BpuMI CCSGG 4 cut(s) 175, 502, 1662, 1663
BsaBI GATNNNNATC 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 968
BsaJI CCNNGG 7 cut(s) 173, 468, 659, 1019, 1192, 1267, 1661
BsaWI WCCGGW 2 cut(s) 541, 1703
BsaXI ACNNNNNCTCC 2 cut(s) 1063, 1093
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 181, 820, 1427
Bse118I RCCGGY 1 cut(s) 1703
Bse1I ACTGG 3 cut(s) 37, 1295, 1451
Bse3DI GCAATG 5 cut(s) 111, 513, 1183, 1386, 1602
Bse8I GATNNNNATC 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 3 cut(s) 660, 1193, 1425
BseDI CCNNGG 7 cut(s) 173, 468, 659, 1019, 1192, 1267, 1661
BseGI GGATG 3 cut(s) 58, 239, 669
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 4 cut(s) 180, 181, 820, 1427
BseMI GCAATG 5 cut(s) 111, 513, 1183, 1386, 1602
BseMII CTCAG 3 cut(s) 707, 1278, 1530
BseNI ACTGG 3 cut(s) 37, 1295, 1451
BseRI GAGGAG 1 cut(s) 198
BseSI GKGCMC 1 cut(s) 837
BseX3I CGGCCG 1 cut(s) 1407
Bsh1236I CGCG 1 cut(s) 821
Bsh1285I CGRYCG 1 cut(s) 1410
BshFI GGCC 4 cut(s) 185, 903, 1409, 1660
BshTI ACCGGT 1 cut(s) 1703
BsiEI CGRYCG 1 cut(s) 1410
BsiHKAI GWGCWC 1 cut(s) 1653
BsiHKCI CYCGRG 1 cut(s) 1661
BsiSI CCGG 5 cut(s) 175, 501, 542, 1662, 1704
BslI CCNNNNNNNGG 4 cut(s) 180, 181, 820, 1427
BsmAI GTCTC 5 cut(s) 976, 1068, 1194, 1283, 1782
BsmI GAATGC 2 cut(s) 357, 436
BsnI GGCC 4 cut(s) 185, 903, 1409, 1660
BsoBI CYCGRG 1 cut(s) 1661
Bsp119I TTCGAA 3 cut(s) 51, 758, 1364
Bsp1286I GDGCHC 2 cut(s) 837, 1653
Bsp13I TCCGGA 1 cut(s) 541
BspACI CCGC 4 cut(s) 585, 821, 1421, 1657
BspANI GGCC 4 cut(s) 185, 903, 1409, 1660
BspCNI CTCAG 3 cut(s) 708, 1279, 1529
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 6 cut(s) 6, 279, 315, 725, 1264, 1811
BspMI ACCTGC 1 cut(s) 1588
BspPI GGATC 9 cut(s) 12, 296, 431, 552, 1257, 1270, 1347, 1804, 1817
BspT104I TTCGAA 3 cut(s) 51, 758, 1364
BsrDI GCAATG 5 cut(s) 111, 513, 1183, 1386, 1602
BsrFI RCCGGY 1 cut(s) 1703
BsrI ACTGG 3 cut(s) 37, 1295, 1451
BssAI RCCGGY 1 cut(s) 1703
BssECI CCNNGG 7 cut(s) 173, 468, 659, 1019, 1192, 1267, 1661
BssNI GRCGYC 1 cut(s) 968
BssT1I CCWWGG 3 cut(s) 468, 1019, 1267
Bst2UI CCWGG 3 cut(s) 660, 1193, 1425
Bst4CI ACNGT 4 cut(s) 1079, 1384, 1396, 1501
Bst6I CTCTTC 2 cut(s) 846, 1192
BstACI GRCGYC 1 cut(s) 968
BstAPI GCANNNNNTGC 1 cut(s) 1628
BstBI TTCGAA 3 cut(s) 51, 758, 1364
BstC8I GCNNGC 5 cut(s) 594, 978, 1137, 1329, 1759
BstDEI CTNAG 4 cut(s) 716, 972, 1287, 1516
BstEII GGTNACC 1 cut(s) 646
BstF5I GGATG 3 cut(s) 58, 239, 669
BstFNI CGCG 1 cut(s) 821
BstH2I RGCGCY 1 cut(s) 1752
BstHHI GCGC 1 cut(s) 1751
BstMAI GTCTC 5 cut(s) 976, 1068, 1194, 1283, 1782
BstMCI CGRYCG 1 cut(s) 1410
BstNI CCWGG 3 cut(s) 660, 1193, 1425
BstNSI RCATGY 4 cut(s) 218, 386, 805, 920
BstPAI GACNNNNGTC 1 cut(s) 1295
BstPI GGTNACC 1 cut(s) 646
BstSCI CCNGG 7 cut(s) 173, 500, 658, 1191, 1423, 1660, 1661
BstSLI GKGCMC 1 cut(s) 837
BstUI CGCG 1 cut(s) 821
BstV2I GAAGAC 1 cut(s) 971
BstX2I RGATCY 3 cut(s) 4, 1262, 1809
BstYI RGATCY 3 cut(s) 4, 1262, 1809
BstZI CGGCCG 1 cut(s) 1407
BsuI GTATCC 2 cut(s) 364, 1513
BsuRI GGCC 4 cut(s) 185, 903, 1409, 1660
BtgZI GCGATG 1 cut(s) 1747
BtrI CACGTC 1 cut(s) 817
BtsCI GGATG 3 cut(s) 58, 239, 669
BtsI GCAGTG 2 cut(s) 377, 1650
BtsIMutI CAGTG 4 cut(s) 377, 713, 1524, 1650
BveI ACCTGC 1 cut(s) 1588
Cac8I GCNNGC 5 cut(s) 594, 978, 1137, 1329, 1759
CfoI GCGC 1 cut(s) 1751
Cfr10I RCCGGY 1 cut(s) 1703
Cfr13I GGNCC 3 cut(s) 183, 901, 1659
Cfr9I CCCGGG 1 cut(s) 1661
CseI GACGC 1 cut(s) 976
Csp6I GTAC 2 cut(s) 955, 1281
CspAI ACCGGT 1 cut(s) 1703
CviAII CATG 6 cut(s) 215, 383, 404, 802, 917, 1795
CviQI GTAC 2 cut(s) 955, 1281
DdeI CTNAG 4 cut(s) 716, 972, 1287, 1516
DraI TTTAAA 1 cut(s) 531
EaeI YGGCCR 1 cut(s) 1407
EagI CGGCCG 1 cut(s) 1407
Eam1104I CTCTTC 2 cut(s) 846, 1192
EarI CTCTTC 2 cut(s) 846, 1192
EclXI CGGCCG 1 cut(s) 1407
Eco130I CCWWGG 3 cut(s) 468, 1019, 1267
Eco52I CGGCCG 1 cut(s) 1407
Eco57I CTGAAG 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 1661
Eco91I GGTNACC 1 cut(s) 646
EcoO65I GGTNACC 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 3 cut(s) 658, 1191, 1423
EcoT14I CCWWGG 3 cut(s) 468, 1019, 1267
EcoT22I ATGCAT 1 cut(s) 712
ErhI CCWWGG 3 cut(s) 468, 1019, 1267
FaeI CATG 6 cut(s) 218, 386, 407, 805, 920, 1798
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FatI CATG 6 cut(s) 214, 382, 403, 801, 916, 1794
FauI CCCGC 1 cut(s) 1428
FauNDI CATATG 1 cut(s) 826
FblI GTMKAC 1 cut(s) 506
FokI GGATG 3 cut(s) 45, 246, 676
FspBI CTAG 3 cut(s) 488, 1028, 1754
GlaI GCGC 1 cut(s) 1750
GsuI CTGGAG 3 cut(s) 429, 1446, 1805
HaeII RGCGCY 1 cut(s) 1752
HaeIII GGCC 4 cut(s) 185, 903, 1409, 1660
HapII CCGG 5 cut(s) 175, 501, 542, 1662, 1704
HgaI GACGC 1 cut(s) 976
HhaI GCGC 1 cut(s) 1751
Hin1I GRCGYC 1 cut(s) 968
Hin1II CATG 6 cut(s) 218, 386, 407, 805, 920, 1798
Hin6I GCGC 1 cut(s) 1749
HinP1I GCGC 1 cut(s) 1749
HindIII AAGCTT 2 cut(s) 330, 974
HinfI GANTC 9 cut(s) 167, 473, 538, 694, 748, 859, 884, 1361, 1585
HpaII CCGG 5 cut(s) 175, 501, 542, 1662, 1704
HphI GGTGA 5 cut(s) 47, 162, 251, 468, 1627
Hpy166II GTNNAC 2 cut(s) 507, 693
Hpy188I TCNGA 9 cut(s) 81, 277, 350, 395, 769, 1288, 1339, 1344, 1548
Hpy188III TCNNGA 9 cut(s) 164, 299, 305, 440, 542, 677, 872, 1252, 1822
Hpy8I GTNNAC 2 cut(s) 507, 693
Hpy99I CGWCG 4 cut(s) 821, 867, 1384, 1700
HpyAV CCTTC 5 cut(s) 18, 47, 1357, 1516, 1800
HpyCH4III ACNGT 4 cut(s) 1079, 1384, 1396, 1501
HpyCH4IV ACGT 4 cut(s) 251, 816, 865, 957
HpyF3I CTNAG 4 cut(s) 716, 972, 1287, 1516
HpySE526I ACGT 4 cut(s) 251, 816, 865, 957
Hsp92I GRCGYC 1 cut(s) 968
Hsp92II CATG 6 cut(s) 218, 386, 407, 805, 920, 1798
HspAI GCGC 1 cut(s) 1749
Kpn2I TCCGGA 1 cut(s) 541
LmnI GCTCC 1 cut(s) 1648
LweI GCATC 3 cut(s) 719, 1033, 1770
MaeI CTAG 3 cut(s) 488, 1028, 1754
MaeII ACGT 4 cut(s) 251, 816, 865, 957
MaeIII GTNAC 7 cut(s) 247, 474, 580, 646, 779, 1396, 1708
MboII GAAGA 9 cut(s) 40, 98, 335, 430, 800, 863, 976, 1164, 1209
MflI RGATCY 3 cut(s) 4, 1262, 1809
MhlI GDGCHC 2 cut(s) 837, 1653
MlyI GAGTC 4 cut(s) 482, 532, 688, 1594
MmeI TCCRAC 1 cut(s) 86
MnlI CCTC 6 cut(s) 219, 266, 713, 1421, 1730, 1829
Mph1103I ATGCAT 1 cut(s) 712
MroI TCCGGA 1 cut(s) 541
MroXI GAANNNNTTC 1 cut(s) 1360
MseI TTAA 8 cut(s) 530, 729, 1067, 1494, 1533, 1626, 1677, 1837
MspA1I CMGCKG 3 cut(s) 26, 68, 119
MspI CCGG 5 cut(s) 175, 501, 542, 1662, 1704
MspR9I CCNGG 7 cut(s) 175, 502, 660, 1193, 1425, 1662, 1663
Mva1269I GAATGC 2 cut(s) 357, 436
MvaI CCWGG 3 cut(s) 660, 1193, 1425
MvnI CGCG 1 cut(s) 821
NciI CCSGG 4 cut(s) 175, 502, 1662, 1663
NdeI CATATG 1 cut(s) 826
NlaIII CATG 6 cut(s) 218, 386, 407, 805, 920, 1798
NlaIV GGNNCC 6 cut(s) 6, 279, 315, 725, 1264, 1811
NmeAIII GCCGAG 1 cut(s) 1165
NmuCI GTSAC 2 cut(s) 474, 580
NsiI ATGCAT 1 cut(s) 712
NspI RCATGY 4 cut(s) 218, 386, 805, 920
NspV TTCGAA 3 cut(s) 51, 758, 1364
PaqCI CACCTGC 1 cut(s) 1588
PciI ACATGT 1 cut(s) 214
PctI GAATGC 2 cut(s) 357, 436
PdmI GAANNNNTTC 1 cut(s) 1360
PfeI GAWTC 5 cut(s) 167, 748, 859, 884, 1361
PflFI GACNNNGTC 2 cut(s) 867, 1205
PinAI ACCGGT 1 cut(s) 1703
PleI GAGTC 4 cut(s) 481, 532, 688, 1593
PpsI GAGTC 4 cut(s) 481, 532, 688, 1593
PscI ACATGT 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 1295
PsiI TTATAA 1 cut(s) 989
Psp1406I AACGTT 1 cut(s) 251
Psp6I CCWGG 3 cut(s) 658, 1191, 1423
PspEI GGTNACC 1 cut(s) 646
PspGI CCWGG 3 cut(s) 658, 1191, 1423
PspN4I GGNNCC 6 cut(s) 6, 279, 315, 725, 1264, 1811
PspPI GGNCC 3 cut(s) 183, 901, 1659
PsuI RGATCY 3 cut(s) 4, 1262, 1809
PsyI GACNNNGTC 2 cut(s) 867, 1205
PvuII CAGCTG 3 cut(s) 26, 68, 119
RsaI GTAC 2 cut(s) 956, 1282
RsaNI GTAC 2 cut(s) 955, 1281
SaqAI TTAA 8 cut(s) 530, 729, 1067, 1494, 1533, 1626, 1677, 1837
Sau96I GGNCC 3 cut(s) 183, 901, 1659
ScaI AGTACT 1 cut(s) 1282
SchI GAGTC 4 cut(s) 482, 532, 688, 1594
ScrFI CCNGG 7 cut(s) 175, 502, 660, 1193, 1425, 1662, 1663
SduI GDGCHC 2 cut(s) 837, 1653
SfaNI GCATC 3 cut(s) 719, 1033, 1770
SfuI TTCGAA 3 cut(s) 51, 758, 1364
SgrAI CRCCGGYG 1 cut(s) 1703
SmaI CCCGGG 1 cut(s) 1663
SrfI GCCCGGGC 1 cut(s) 1663
SsiI CCGC 4 cut(s) 585, 821, 1421, 1657
SspI AATATT 2 cut(s) 103, 1682
SspMI CTAG 3 cut(s) 488, 1028, 1754
StyD4I CCNGG 7 cut(s) 173, 500, 658, 1191, 1423, 1660, 1661
StyI CCWWGG 3 cut(s) 468, 1019, 1267
TaaI ACNGT 4 cut(s) 1079, 1384, 1396, 1501
TaiI ACGT 4 cut(s) 254, 819, 868, 960
TaqI TCGA 8 cut(s) 51, 165, 669, 758, 786, 862, 1251, 1364
TaqII GACCGA 1 cut(s) 877
TatI WGTACW 1 cut(s) 1280
TauI GCSGC 2 cut(s) 824, 1660
TfiI GAWTC 5 cut(s) 167, 748, 859, 884, 1361
Tru1I TTAA 8 cut(s) 530, 729, 1067, 1494, 1533, 1626, 1677, 1837
Tru9I TTAA 8 cut(s) 530, 729, 1067, 1494, 1533, 1626, 1677, 1837
TscAI CASTG 4 cut(s) 384, 720, 1524, 1650
TseFI GTSAC 2 cut(s) 474, 580
Tsp45I GTSAC 2 cut(s) 474, 580
TspDTI ATGAA 7 cut(s) 33, 342, 444, 696, 807, 1430, 1551
TspMI CCCGGG 1 cut(s) 1661
TspRI CASTG 4 cut(s) 384, 720, 1524, 1650
Tth111I GACNNNGTC 2 cut(s) 867, 1205
XapI RAATTY 5 cut(s) 122, 630, 754, 770, 1256
XceI RCATGY 4 cut(s) 218, 386, 805, 920
XcmI CCANNNNNNNNNTGG 1 cut(s) 1609
XmaI CCCGGG 1 cut(s) 1661
XmiI GTMKAC 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 1360
XspI CTAG 3 cut(s) 488, 1028, 1754
ZrmI AGTACT 1 cut(s) 1282
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.