Rh2CG213100

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000319
Physical Location & Seq
Forward (+)
20962267 .. 20962944
678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG213100.1

Sequence Viewer

Length: 636 bp
ATGGATCCTTCGCTGTATGAGGCAGCAGCATCAGGTGATCTTCGTTTTCTCAAAGAAGTTGGAGATGGAGATAGATCAGTTGATATTCTCTTTCAGAAGACCCCTAAAAATAACAATGTTCTTCACATAGCTGCTCAGTTCATGCAAACACAATTCTTCAAGGAATTCCCAAATCAGATTCAATCTCCGTTGTTTTGGGCCACCAACACCAGGGGTGACACTCCCATGCACATTGCTGCTAGGGTAGGTTGTGTTGAACTAGTTGAGTTCCTCATCGATCACGCAAAAAAGTTACAAACTGATGCAGCAGATGAGGAGACCGGACCAGCCGATGCTGAAGCTTATAAAGAGTCACTTCGAATGACTAATTCGGAAAAAGATACAGCTTTGCATGTTGCTGTTAAAAGCGGGTACCATGGTGTTGTCATTCTGTTAATGGATGCTGATCCTGAATTGTGCTGTTATACCAATAACGCCAAAGAATCTCCTTTGTTCTTAGCTGTTAGCAAGGGGTTTCCAGAAATTGGTTCTTGTATTTTGGACAGGTCTTCAGCATCTCTTTCTTTCGAGGGGGTTAAAGGTGTCACAGCAAACCCGCAATTCAACCTGCAAAGGGACTTGCACTATATGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

211

Amino Acids

23.35

Weight (kDa)

5.0

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 6 - 97 7.1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 120 - 180 8.1e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 615
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 411
AccB7I CCANNNNNTGG 1 cut(s) 524
AciI CCGC 2 cut(s) 408, 596
AclWI GGATC 2 cut(s) 12, 440
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 2 cut(s) 357, 534
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 3 cut(s) 210, 524, 613
AgsI TTSAA 4 cut(s) 160, 182, 257, 604
AhlI ACTAGT 1 cut(s) 259
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 4 cut(s) 131, 341, 386, 500
AluI AGCT 4 cut(s) 131, 341, 386, 500
Alw26I GTCTC 1 cut(s) 311
AlwI GGATC 2 cut(s) 12, 440
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 5 cut(s) 23, 26, 131, 236, 305
ApoI RAATTY 1 cut(s) 164
Asp718I GGTACC 1 cut(s) 411
AspS9I GGNCC 2 cut(s) 198, 323
AsuHPI GGTGA 2 cut(s) 47, 227
AsuII TTCGAA 1 cut(s) 358
AvaII GGWCC 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 403, 436
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 411
BbsI GAAGAC 2 cut(s) 104, 540
BbvI GCAGC 5 cut(s) 35, 38, 118, 223, 317
BccI CCATC 1 cut(s) 59
BciT130I CCWGG 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 311
BcuI ACTAGT 1 cut(s) 259
BfaI CTAG 2 cut(s) 240, 260
BfuAI ACCTGC 1 cut(s) 615
BisI GCNGC 5 cut(s) 24, 27, 132, 237, 306
BlsI GCNGC 5 cut(s) 25, 28, 133, 238, 307
Bme1390I CCNGG 1 cut(s) 211
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 198, 323
BmiI GGNNCC 2 cut(s) 6, 413
BmrFI CCNGG 1 cut(s) 211
BmsI GCATC 5 cut(s) 38, 292, 322, 430, 563
BpiI GAAGAC 2 cut(s) 104, 540
Bpu14I TTCGAA 1 cut(s) 358
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 210, 415
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 524, 613
Bse3DI GCAATG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 1 cut(s) 211
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 210, 415
BseGI GGATG 1 cut(s) 445
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 3 cut(s) 210, 524, 613
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 149
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 5 cut(s) 35, 38, 118, 223, 317
BshFI GGCC 1 cut(s) 200
BshNI GGYRCC 1 cut(s) 411
BshVI ATCGAT 1 cut(s) 276
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 3 cut(s) 210, 524, 613
BsmAI GTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 629
BsnI GGCC 1 cut(s) 200
Bso31I GGTCTC 1 cut(s) 311
Bsp119I TTCGAA 1 cut(s) 358
Bsp143I GATC 5 cut(s) 4, 37, 74, 277, 445
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 2 cut(s) 408, 596
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 148
BspDI ATCGAT 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 6, 413
BspMI ACCTGC 1 cut(s) 615
BspPI GGATC 2 cut(s) 12, 440
BspT104I TTCGAA 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 411
BspTNI GGTCTC 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 210, 415
BssMI GATC 5 cut(s) 4, 37, 74, 277, 445
BssT1I CCWWGG 1 cut(s) 415
Bst2UI CCWGG 1 cut(s) 211
BstBI TTCGAA 1 cut(s) 358
BstDEI CTNAG 2 cut(s) 135, 496
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 1 cut(s) 445
BstKTI GATC 5 cut(s) 7, 40, 77, 280, 448
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 5 cut(s) 4, 37, 74, 277, 445
BstNI CCWGG 1 cut(s) 211
BstNSI RCATGY 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 209
BstV1I GCAGC 5 cut(s) 35, 38, 118, 223, 317
BstV2I GAAGAC 2 cut(s) 104, 540
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 1 cut(s) 200
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 1 cut(s) 445
BveI ACCTGC 1 cut(s) 615
Cfr13I GGNCC 2 cut(s) 198, 323
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 1 cut(s) 412
CviAII CATG 4 cut(s) 142, 226, 392, 416
CviJI RGCY 6 cut(s) 131, 200, 329, 341, 386, 500
CviKI_1 RGCY 6 cut(s) 131, 200, 329, 341, 386, 500
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 2 cut(s) 135, 496
DpnI GATC 5 cut(s) 6, 39, 76, 279, 447
DpnII GATC 5 cut(s) 4, 37, 74, 277, 445
Eco130I CCWWGG 1 cut(s) 415
Eco31I GGTCTC 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 323
Eco57I CTGAAG 2 cut(s) 357, 534
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 4 cut(s) 145, 229, 395, 419
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 1 cut(s) 629
FatI CATG 4 cut(s) 141, 225, 391, 415
FauI CCCGC 2 cut(s) 401, 603
Fnu4HI GCNGC 5 cut(s) 24, 27, 132, 237, 306
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 5 cut(s) 24, 27, 132, 237, 306
FspBI CTAG 2 cut(s) 240, 260
GluI GCNGC 5 cut(s) 24, 27, 132, 237, 306
HaeIII GGCC 1 cut(s) 200
HapII CCGG 1 cut(s) 321
Hin1II CATG 4 cut(s) 145, 229, 395, 419
HindIII AAGCTT 1 cut(s) 339
HinfI GANTC 3 cut(s) 178, 350, 482
HpaII CCGG 1 cut(s) 321
HphI GGTGA 2 cut(s) 47, 227
Hpy188I TCNGA 3 cut(s) 96, 177, 373
Hpy188III TCNNGA 2 cut(s) 449, 518
HpyAV CCTTC 1 cut(s) 18
HpyCH4V TGCA 6 cut(s) 145, 229, 305, 391, 610, 622
HpyF3I CTNAG 2 cut(s) 135, 496
Hsp92II CATG 4 cut(s) 145, 229, 395, 419
KpnI GGTACC 1 cut(s) 415
Kzo9I GATC 5 cut(s) 4, 37, 74, 277, 445
LpnPI CCDG 9 cut(s) 18, 196, 223, 334, 339, 462, 529, 531, 620
Lsp1109I GCAGC 5 cut(s) 35, 38, 118, 223, 317
LweI GCATC 5 cut(s) 38, 292, 322, 430, 563
MaeI CTAG 2 cut(s) 240, 260
MaeIII GTNAC 4 cut(s) 215, 291, 351, 583
MalI GATC 5 cut(s) 6, 39, 76, 279, 447
MboI GATC 5 cut(s) 4, 37, 74, 277, 445
MboII GAAGA 5 cut(s) 32, 109, 113, 148, 540
MflI RGATCY 1 cut(s) 4
MluCI AATT 6 cut(s) 152, 164, 367, 452, 522, 599
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 40
MnlI CCTC 4 cut(s) 13, 281, 307, 562
MseI TTAA 4 cut(s) 402, 434, 576, 634
MslI CAYNNNNRTG 1 cut(s) 224
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 211
MvaI CCWGG 1 cut(s) 211
NcoI CCATGG 1 cut(s) 415
NdeII GATC 5 cut(s) 4, 37, 74, 277, 445
NlaIII CATG 4 cut(s) 145, 229, 395, 419
NlaIV GGNNCC 2 cut(s) 6, 413
NmuCI GTSAC 3 cut(s) 215, 351, 583
NspI RCATGY 1 cut(s) 395
NspV TTCGAA 1 cut(s) 358
PfeI GAWTC 2 cut(s) 178, 482
PflMI CCANNNNNTGG 1 cut(s) 524
PkrI GCNGC 5 cut(s) 25, 28, 133, 238, 307
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
PsiI TTATAA 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 209
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 2 cut(s) 6, 413
PspPI GGNCC 2 cut(s) 198, 323
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 224
SaqAI TTAA 4 cut(s) 402, 434, 576, 634
SatI GCNGC 5 cut(s) 24, 27, 132, 237, 306
Sau3AI GATC 5 cut(s) 4, 37, 74, 277, 445
Sau96I GGNCC 2 cut(s) 198, 323
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 211
SetI ASST 9 cut(s) 37, 133, 250, 343, 388, 502, 548, 583, 609
SfaNI GCATC 5 cut(s) 38, 292, 322, 430, 563
SfuI TTCGAA 1 cut(s) 358
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 224
SpeI ACTAGT 1 cut(s) 259
Sse9I AATT 6 cut(s) 152, 164, 367, 452, 522, 599
SsiI CCGC 2 cut(s) 408, 596
SspMI CTAG 2 cut(s) 240, 260
StyD4I CCNGG 1 cut(s) 209
StyI CCWWGG 1 cut(s) 415
TaqI TCGA 3 cut(s) 276, 358, 567
TasI AATT 6 cut(s) 152, 164, 367, 452, 522, 599
TfiI GAWTC 2 cut(s) 178, 482
Tru1I TTAA 4 cut(s) 402, 434, 576, 634
Tru9I TTAA 4 cut(s) 402, 434, 576, 634
TseFI GTSAC 3 cut(s) 215, 351, 583
TseI GCWGC 5 cut(s) 23, 26, 131, 236, 305
Tsp45I GTSAC 3 cut(s) 215, 351, 583
TspDTI ATGAA 1 cut(s) 130
TspGWI ACGGA 1 cut(s) 177
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 164
XceI RCATGY 1 cut(s) 395
XspI CTAG 2 cut(s) 240, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.