Rh5BG188600

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000319
Physical Location & Seq
Forward (+)
20682664 .. 20691388
8725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG188600.1

Sequence Viewer

Length: 1569 bp
ATGGATCCTCGTCTATATGAGGCGGTAATATCCGGTGACGTTGATTTCTTGAAAAGAAAAATCGGAGATGGTGATCCTCTCAGTCAGAGAACACCTAAAGACAACAATATTCTTCACCTTGCTGCTGAATTCAAACACATAAACGTCTTCACGGAAGTACCACAAGGTGATCTATATTCTCCGCTGTTTTGGGCTGCCAACAAGTTTGGCGATACCCCTTTACACGTTGCTGCAAGAGTAGGTTGCAATGAGGTAATAACATTCCTCATCGAACACTCGAAGAAAACACTACCCGGTGGAGCAGCTGATGTGGAAAGTGGACCAGCTGATGCTGAAGCTTATAAAGAGCTGCTTCGTGTTGCTAATTTGCGGAAGGATACAGCATTTCATGTAGCTGTCCAATACGGTTACAATGAAGTGGTGAATTCGTTAATGGTGGCTGATCCCGAATTGTGCTGTTTTACTAACAGCGCCAATGAGTCACCACTCTTCATAGCTGTTCGCAAGGGCTTTTACTCCATAGCGCGTTCTATTTTAGTGAATTGTCCAATTTCTCCTTCTTTTGAAGGAACCGACGGTGTGACAGCTTTGCATGCCGCAGTAACTCGCCTGCGCGTCAGCAGAAGCCTCGTGAAGATTATGGTGTCCAAAAAAGCAGATATAATCGAAAAAACTGATGCAATTGGATGGACACCCTTGCACTACGCAGCATTGAGAGGGAACCTTAAGATAACTCGGCTACTGCTAATAGAGAATAGTTCTGTCTCTTATACTCTAGACAAGTCTGAAATGTCAGCTCTCCATATCGCGGCTTGTGCAGGAAGCACTAAAGTATTGAAAGAGTTGATTCAATGTCGGCCTGATACTTGTGATTTACTCAATGATAAGGGCCAAAACATTCTCCATGCTGCAGTCTTAGGTCGACAAAGATTTATTATCAGGTACATATTGAAGAATCCTAAGCTTTCCAGACTTATAAATGAAGCGGATAAAGAAGGGAACACTCCTTTACATCTAGCCGTCATTTATTACAAAGGAGACAGACCTCTGATTAGATGTTTGGCACTCAATCCTAGAGTTGATCAGACTGTTATAAATAACGAATTCTCACATGCGGTCGACATCCTCACTAGCCAGAATTTTAAACAGGGTTTTCTTACTTACGATCCTCGCGGTTTTGGCTTCGAGAGAAATGTTGGCGTGCCAGCTTTTCACAAAAAAATTCTTCATGAAATCATGGCATTTGAACCTAAGGAGAACAATACGTCGAGCCCACCAACAAGTAGAGAAAATTGGCAAGCTAATAATGGAGATAAAGCTTTGAAAACCTATGATACCACTGAATTGGTAGCGGTGCTTATTGCGATGGTAACATTTGCTGCAGCCTTTACCATACCTGGAGGATTTAAAAGTGACGGCACACCAGTTTTATACAGCAAAGCATTCTTCCCAGTGTTTCTGGTATCAATTATCAAACGCTTTCTCTTTCTTCACATCGATTTTGGTTCTGTGTCTTCATTTTTTGAGGGTCACTGCTGTCGACGTACCATCGATGGGACGGATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

522

Amino Acids

57.84

Weight (kDa)

8.21

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 93 8.7e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 129 - 222 4e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 163 - 252 2e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 197 - 286 1.7e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 210 - 249 4.3e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 267 - 355 4.1e-11 Ankyrin repeats (3 copies)
PGG PF13962 442 - 489 2e-10 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000319)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18590 FvH4_1g18590 FvH4_3g16140 FvH4_3g16141 FvH4_3g16160
malus_domestica MD02G1193600.v1.1 MD04G1020900.v1.1 MD04G1021000.v1.1 MD05G1212100.v1.1 MD05G1212300.v1.1 MD05G1213100.v1.1 MD05G1213400.v1.1 MD10G1197300.v1.1
prunus_persica Prupe.4G144500_v2.0.a1 Prupe.4G144600_v2.0.a1 Prupe.4G144800_v2.0.a1 Prupe.4G144900_v2.0.a1 Prupe.4G145100_v2.0.a1 Prupe.4G145300_v2.0.a1
pyrus_communis pycom02g15730 pycom02g15740 pycom05g19670 pycom05g19680 pycom10g17020 pycom11g27250
rosa_chinensis RchiOBHm_Chr2g0109301 RchiOBHm_Chr2g0109321 RchiOBHm_Chr2g0109341 RchiOBHm_Chr2g0109381 RchiOBHm_Chr3g0482301 RchiOBHm_Chr5g0027111 RchiOBHm_Chr5g0027121 RchiOBHm_Chr5g0027141 RchiOBHm_Chr5g0027181 RchiOBHm_Chr5g0027241
rosa_laevigata RLG00000017731 RLG00000017732 RLG00000032990 RLG00000032992 RLG00000032993 RLG00000032995 RLG00000032997 RLG00000033002
rosa_multiflora Rmu_sc0000135.1_g000002 Rmu_sc0000332.1_g000010 Rmu_sc0000882.1_g000028 Rmu_sc0000882.1_g000032 Rmu_sc0000882.1_g000033 Rmu_sc0000882.1_g000039 Rmu_sc0000882.1_g000043 Rmu_sc0000882.1_g000046 Rmu_sc0006065.1_g000003 Rmu_sc0006065.1_g000012 Rmu_sc0006065.1_g000018 Rmu_sc0006065.1_g000019 Rmu_sc0006065.1_g000022 Rmu_sc0007497.1_g000002 Rmu_sc0013610.1_g000011 Rmu_sc0015338.1_g000006 Rmu_ssc0000357.1_g000035
rosa_roxburghii Rroxscaffold_1G00052250 Rroxscaffold_1G00052280 Rroxscaffold_1G00052290 Rroxscaffold_1G00052310 Rroxscaffold_1G00052330 Rroxscaffold_1G00052360 Rroxscaffold_1G00052380 Rroxscaffold_1G00052390 Rroxscaffold_2G00134310 Rroxscaffold_2G00134330
rosa_rugosa Rorug02G0159400 Rorug05G0097300 Rorug05G0097500 Rorug05G0097600 Rorug05G0097700 Rorug05G0097700 Rorug05G0097700 Rorug05G0122900
rosa_samantha Rh2AG210900 Rh2AG211200 Rh2AG211500 Rh2BG221200 Rh2BG221800 Rh2CG213100 Rh2CG213200 Rh2CG213600 Rh3CG277900 Rh3CG278000 Rh3CG278600 Rh3DG274600 Rh5BG188600 Rh5BG188700 Rh5BG188900 Rh5BG189100 Rh5BG189200 Rh5BG189400 Rh5CG208400 Rh5CG208500 Rh5CG208700 Rh5CG209000 Rh5CG209100 Rh5CG209400 Rh5CG209700 Rh5CG209800 Rh5CG209900 Rh5CG210000 Rh5DG190500 Rh5DG190700 Rh5DG191200 Rh5DG191500
rosa_wichuraiana Rw3G022160 Rw5G017410 Rw5G017420 Rw5G017450 Rw5G017460 Rw5G017470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 342, 977, 1094
AccI GTMKAC 3 cut(s) 922, 1119, 1540
AccII CGCG 4 cut(s) 526, 615, 809, 1173
AciI CCGC 9 cut(s) 23, 182, 370, 597, 809, 986, 1115, 1173, 1352
AclWI GGATC 5 cut(s) 12, 68, 437, 1160, 1556
AcsI RAATTY 5 cut(s) 128, 424, 1103, 1138, 1221
AcuI CTGAAG 1 cut(s) 354
AdeI CACNNNGTG 1 cut(s) 167
AfaI GTAC 3 cut(s) 159, 944, 1546
AfiI CCNNNNNNNGG 2 cut(s) 808, 1554
AflII CTTAAG 1 cut(s) 725
AflIII ACRYGT 1 cut(s) 223
AgsI TTSAA 8 cut(s) 52, 133, 566, 838, 851, 952, 1247, 1324
AjnI CCWGG 1 cut(s) 1396
AjuI GAANNNNNNNTTGG 2 cut(s) 541, 573
Alw26I GTCTC 2 cut(s) 769, 1032
AlwI GGATC 5 cut(s) 12, 68, 437, 1160, 1556
AoxI GGCC 2 cut(s) 857, 889
ApeKI GCWGC 9 cut(s) 122, 194, 230, 302, 349, 707, 908, 1379, 1382
ApoI RAATTY 5 cut(s) 128, 424, 1103, 1138, 1221
AspLEI GCGC 3 cut(s) 473, 526, 615
AspS9I GGNCC 2 cut(s) 320, 889
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 6 cut(s) 47, 83, 107, 179, 433, 474
AvaII GGWCC 1 cut(s) 320
AxyI CCTNAGG 1 cut(s) 1251
BaeI ACNNNNGTAYC 2 cut(s) 141, 174
BamHI GGATCC 2 cut(s) 4, 1561
BanII GRGCYC 1 cut(s) 1274
BauI CACGAG 1 cut(s) 629
BbsI GAAGAC 2 cut(s) 139, 1506
BbvI GCAGC 9 cut(s) 109, 181, 217, 314, 336, 719, 895, 1366, 1394
BccI CCATC 5 cut(s) 62, 681, 1360, 1547, 1556
BceAI ACGGC 2 cut(s) 1004, 1432
BcgI CGANNNNNNTGC 2 cut(s) 610, 644
BciT130I CCWGG 1 cut(s) 1398
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 2 cut(s) 769, 1032
BfaI CTAG 4 cut(s) 776, 1016, 1074, 1131
BfmI CTRYAG 2 cut(s) 909, 1380
BfoI RGCGCY 1 cut(s) 474
BfrI CTTAAG 1 cut(s) 725
BfuI GTATCC 1 cut(s) 370
Bme1390I CCNGG 2 cut(s) 294, 1398
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 2 cut(s) 320, 889
BmiI GGNNCC 4 cut(s) 6, 571, 722, 1563
BmrFI CCNGG 2 cut(s) 294, 1398
BmrI ACTGGG 1 cut(s) 1445
BmsI GCATC 2 cut(s) 319, 667
BmuI ACTGGG 1 cut(s) 1445
BpiI GAAGAC 2 cut(s) 139, 1506
BpmI CTGGAG 1 cut(s) 1419
Bpu10I CCTNAGC 1 cut(s) 960
BpuMI CCSGG 1 cut(s) 294
Bsa29I ATCGAT 2 cut(s) 1497, 1551
BsaBI GATNNNNATC 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 808, 1554
Bse1I ACTGG 2 cut(s) 1424, 1451
Bse21I CCTNAGG 1 cut(s) 1251
Bse3DI GCAATG 1 cut(s) 253
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 1 cut(s) 1398
BseCI ATCGAT 2 cut(s) 1497, 1551
BseGI GGATG 2 cut(s) 692, 1122
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 2 cut(s) 808, 1554
BseMI GCAATG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 94
BseNI ACTGG 2 cut(s) 1424, 1451
BseXI GCAGC 9 cut(s) 109, 181, 217, 314, 336, 719, 895, 1366, 1394
BsgI GTGCAG 1 cut(s) 837
Bsh1236I CGCG 4 cut(s) 526, 615, 809, 1173
Bsh1285I CGRYCG 1 cut(s) 1119
BshFI GGCC 2 cut(s) 859, 891
BshVI ATCGAT 2 cut(s) 1497, 1551
BsiEI CGRYCG 1 cut(s) 1119
BsiSI CCGG 2 cut(s) 33, 294
BslI CCNNNNNNNGG 2 cut(s) 808, 1554
BsmAI GTCTC 2 cut(s) 769, 1032
BsmI GAATGC 1 cut(s) 1442
BsnI GGCC 2 cut(s) 859, 891
Bsp1286I GDGCHC 1 cut(s) 1274
Bsp143I GATC 7 cut(s) 4, 73, 169, 442, 1081, 1165, 1561
BspACI CCGC 9 cut(s) 23, 182, 370, 597, 809, 986, 1115, 1173, 1352
BspANI GGCC 2 cut(s) 859, 891
BspCNI CTCAG 1 cut(s) 93
BspDI ATCGAT 2 cut(s) 1497, 1551
BspFNI CGCG 4 cut(s) 526, 615, 809, 1173
BspHI TCATGA 1 cut(s) 1228
BspLI GGNNCC 4 cut(s) 6, 571, 722, 1563
BspMAI CTGCAG 2 cut(s) 913, 1384
BspPI GGATC 5 cut(s) 12, 68, 437, 1160, 1556
BspTI CTTAAG 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 253
BsrI ACTGG 2 cut(s) 1424, 1451
BssMI GATC 7 cut(s) 4, 73, 169, 442, 1081, 1165, 1561
BssSI CACGAG 1 cut(s) 629
Bst2BI CACGAG 1 cut(s) 629
Bst2UI CCWGG 1 cut(s) 1398
Bst4CI ACNGT 3 cut(s) 407, 578, 1090
Bst6I CTCTTC 1 cut(s) 494
BstAFI CTTAAG 1 cut(s) 725
BstC8I GCNNGC 5 cut(s) 594, 611, 1202, 1206, 1299
BstDEI CTNAG 5 cut(s) 80, 916, 960, 1251, 1566
BstF5I GGATG 2 cut(s) 692, 1122
BstFNI CGCG 4 cut(s) 526, 615, 809, 1173
BstH2I RGCGCY 1 cut(s) 474
BstHHI GCGC 3 cut(s) 473, 526, 615
BstKTI GATC 7 cut(s) 7, 76, 172, 445, 1084, 1168, 1564
BstMAI GTCTC 2 cut(s) 769, 1032
BstMBI GATC 7 cut(s) 4, 73, 169, 442, 1081, 1165, 1561
BstMCI CGRYCG 1 cut(s) 1119
BstMWI GCNNNNNNNGC 3 cut(s) 593, 815, 1179
BstNI CCWGG 1 cut(s) 1398
BstNSI RCATGY 2 cut(s) 596, 1115
BstSCI CCNGG 2 cut(s) 292, 1396
BstSFI CTRYAG 2 cut(s) 909, 1380
BstUI CGCG 4 cut(s) 526, 615, 809, 1173
BstV1I GCAGC 9 cut(s) 109, 181, 217, 314, 336, 719, 895, 1366, 1394
BstV2I GAAGAC 2 cut(s) 139, 1506
BstX2I RGATCY 2 cut(s) 4, 1561
BstXI CCANNNNNNTGG 1 cut(s) 1345
BstYI RGATCY 2 cut(s) 4, 1561
Bsu15I ATCGAT 2 cut(s) 1497, 1551
Bsu36I CCTNAGG 1 cut(s) 1251
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 2 cut(s) 859, 891
BsuTUI ATCGAT 2 cut(s) 1497, 1551
BtgZI GCGATG 1 cut(s) 1379
BtsCI GGATG 2 cut(s) 692, 1122
BtsI GCAGTG 1 cut(s) 1531
BtsIMutI CAGTG 3 cut(s) 1338, 1458, 1531
Cac8I GCNNGC 5 cut(s) 594, 611, 1202, 1206, 1299
CciI TCATGA 1 cut(s) 1228
CfoI GCGC 3 cut(s) 473, 526, 615
Cfr13I GGNCC 2 cut(s) 320, 889
ClaI ATCGAT 2 cut(s) 1497, 1551
CseI GACGC 1 cut(s) 604
Csp6I GTAC 3 cut(s) 158, 943, 1545
CviAII CATG 6 cut(s) 389, 593, 905, 1112, 1229, 1237
CviQI GTAC 3 cut(s) 158, 943, 1545
DdeI CTNAG 5 cut(s) 80, 916, 960, 1251, 1566
DpnI GATC 7 cut(s) 6, 75, 171, 444, 1083, 1167, 1563
DpnII GATC 7 cut(s) 4, 73, 169, 442, 1081, 1165, 1561
DraI TTTAAA 2 cut(s) 1144, 1408
DraIII CACNNNGTG 1 cut(s) 167
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
Eco24I GRGCYC 1 cut(s) 1274
Eco47I GGWCC 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 354
Eco81I CCTNAGG 1 cut(s) 1251
EcoRI GAATTC 3 cut(s) 128, 424, 1103
EcoRII CCWGG 1 cut(s) 1396
EcoT38I GRGCYC 1 cut(s) 1274
FaeI CATG 6 cut(s) 392, 596, 908, 1115, 1232, 1240
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FatI CATG 6 cut(s) 388, 592, 904, 1111, 1228, 1236
FbaI TGATCA 1 cut(s) 1081
FblI GTMKAC 3 cut(s) 922, 1119, 1540
FokI GGATG 2 cut(s) 699, 1109
FriOI GRGCYC 1 cut(s) 1274
FspBI CTAG 4 cut(s) 776, 1016, 1074, 1131
GlaI GCGC 3 cut(s) 472, 525, 614
GsuI CTGGAG 1 cut(s) 1419
HaeII RGCGCY 1 cut(s) 474
HaeIII GGCC 2 cut(s) 859, 891
HapII CCGG 2 cut(s) 33, 294
HgaI GACGC 1 cut(s) 604
HhaI GCGC 3 cut(s) 473, 526, 615
Hin1II CATG 6 cut(s) 392, 596, 908, 1115, 1232, 1240
Hin6I GCGC 3 cut(s) 471, 524, 613
HinP1I GCGC 3 cut(s) 471, 524, 613
HincII GTYRAC 3 cut(s) 923, 1120, 1541
HindII GTYRAC 3 cut(s) 923, 1120, 1541
HindIII AAGCTT 3 cut(s) 336, 962, 1317
HinfI GANTC 3 cut(s) 479, 847, 955
HpaII CCGG 2 cut(s) 33, 294
HphI GGTGA 6 cut(s) 47, 83, 107, 179, 433, 474
Hpy166II GTNNAC 4 cut(s) 320, 923, 1120, 1541
Hpy188I TCNGA 5 cut(s) 65, 87, 787, 1050, 1086
Hpy188III TCNNGA 7 cut(s) 49, 446, 631, 776, 969, 1186, 1229
Hpy8I GTNNAC 4 cut(s) 320, 923, 1120, 1541
Hpy99I CGWCG 3 cut(s) 578, 1270, 1545
HpyAV CCTTC 4 cut(s) 367, 560, 567, 989
HpyCH4III ACNGT 3 cut(s) 407, 578, 1090
HpyCH4IV ACGT 5 cut(s) 39, 144, 225, 1265, 1543
HpyCH4V TGCA 8 cut(s) 233, 246, 592, 680, 700, 818, 911, 1382
HpyF10VI GCNNNNNNNGC 3 cut(s) 593, 815, 1179
HpyF3I CTNAG 5 cut(s) 80, 916, 960, 1251, 1566
HpySE526I ACGT 5 cut(s) 39, 144, 225, 1265, 1543
Hsp92II CATG 6 cut(s) 392, 596, 908, 1115, 1232, 1240
HspAI GCGC 3 cut(s) 471, 524, 613
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 7 cut(s) 4, 73, 169, 442, 1081, 1165, 1561
LmnI GCTCC 1 cut(s) 299
Lsp1109I GCAGC 9 cut(s) 109, 181, 217, 314, 336, 719, 895, 1366, 1394
LweI GCATC 2 cut(s) 319, 667
MaeI CTAG 4 cut(s) 776, 1016, 1074, 1131
MaeII ACGT 5 cut(s) 39, 144, 225, 1265, 1543
MaeIII GTNAC 8 cut(s) 35, 407, 480, 580, 601, 1369, 1412, 1529
MalI GATC 7 cut(s) 6, 75, 171, 444, 1083, 1167, 1563
MboI GATC 7 cut(s) 4, 73, 169, 442, 1081, 1165, 1561
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 2 cut(s) 4, 1561
MhlI GDGCHC 1 cut(s) 1274
MlyI GAGTC 1 cut(s) 488
MseI TTAA 4 cut(s) 431, 726, 1143, 1407
MspA1I CMGCKG 3 cut(s) 184, 305, 326
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 2 cut(s) 33, 294
MspR9I CCNGG 2 cut(s) 294, 1398
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 1 cut(s) 1442
MvaI CCWGG 1 cut(s) 1398
MvnI CGCG 4 cut(s) 526, 615, 809, 1173
MwoI GCNNNNNNNGC 3 cut(s) 593, 815, 1179
NciI CCSGG 1 cut(s) 294
NdeII GATC 7 cut(s) 4, 73, 169, 442, 1081, 1165, 1561
NlaIII CATG 6 cut(s) 392, 596, 908, 1115, 1232, 1240
NlaIV GGNNCC 4 cut(s) 6, 571, 722, 1563
NmeAIII GCCGAG 1 cut(s) 715
NmuCI GTSAC 5 cut(s) 35, 480, 580, 1412, 1529
NspI RCATGY 2 cut(s) 596, 1115
PaeI GCATGC 1 cut(s) 596
PagI TCATGA 1 cut(s) 1228
PctI GAATGC 1 cut(s) 1442
PfeI GAWTC 2 cut(s) 847, 955
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PsiI TTATAA 3 cut(s) 342, 977, 1094
Psp6I CCWGG 1 cut(s) 1396
PspGI CCWGG 1 cut(s) 1396
PspN4I GGNNCC 4 cut(s) 6, 571, 722, 1563
PspPI GGNCC 2 cut(s) 320, 889
PstI CTGCAG 2 cut(s) 913, 1384
PsuI RGATCY 2 cut(s) 4, 1561
PvuII CAGCTG 2 cut(s) 305, 326
RsaI GTAC 3 cut(s) 159, 944, 1546
RsaNI GTAC 3 cut(s) 158, 943, 1545
SalI GTCGAC 3 cut(s) 921, 1118, 1539
SaqAI TTAA 4 cut(s) 431, 726, 1143, 1407
Sau3AI GATC 7 cut(s) 4, 73, 169, 442, 1081, 1165, 1561
Sau96I GGNCC 2 cut(s) 320, 889
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 2 cut(s) 294, 1398
SduI GDGCHC 1 cut(s) 1274
SfaNI GCATC 2 cut(s) 319, 667
SfcI CTRYAG 2 cut(s) 909, 1380
SinI GGWCC 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SphI GCATGC 1 cut(s) 596
SsiI CCGC 9 cut(s) 23, 182, 370, 597, 809, 986, 1115, 1173, 1352
SspI AATATT 1 cut(s) 109
SspMI CTAG 4 cut(s) 776, 1016, 1074, 1131
StyD4I CCNGG 2 cut(s) 292, 1396
TaaI ACNGT 3 cut(s) 407, 578, 1090
TaiI ACGT 5 cut(s) 42, 147, 228, 1268, 1546
TauI GCSGC 2 cut(s) 599, 812
TfiI GAWTC 2 cut(s) 847, 955
Tru1I TTAA 4 cut(s) 431, 726, 1143, 1407
Tru9I TTAA 4 cut(s) 431, 726, 1143, 1407
TscAI CASTG 3 cut(s) 1345, 1458, 1538
TseFI GTSAC 5 cut(s) 35, 480, 580, 1412, 1529
TseI GCWGC 9 cut(s) 122, 194, 230, 302, 349, 707, 908, 1379, 1382
Tsp45I GTSAC 5 cut(s) 35, 480, 580, 1412, 1529
TspDTI ATGAA 7 cut(s) 377, 429, 481, 996, 1217, 1245, 1506
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 3 cut(s) 1345, 1458, 1538
Vha464I CTTAAG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 320
XapI RAATTY 5 cut(s) 128, 424, 1103, 1138, 1221
XbaI TCTAGA 1 cut(s) 775
XceI RCATGY 2 cut(s) 596, 1115
XmiI GTMKAC 3 cut(s) 922, 1119, 1540
XspI CTAG 4 cut(s) 776, 1016, 1074, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.